AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31637


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31637.t1 MTR_1g008000 74.708 257 20 2 6 262 58 269 2.52e-129 367
MS.gene31637.t1 MTR_1g037540 43.333 270 123 15 8 261 145 400 2.41e-46 159
MS.gene31637.t1 MTR_1g023400 42.366 262 122 12 7 261 113 352 9.92e-42 146
MS.gene31637.t1 MTR_4g080200 40.000 260 136 11 7 261 105 349 5.79e-41 144
MS.gene31637.t1 MTR_1g022200 40.146 274 130 13 3 262 117 370 2.14e-38 138
MS.gene31637.t1 MTR_1g009110 38.060 268 143 13 2 262 128 379 2.00e-32 122
MS.gene31637.t1 MTR_1g023430 44.056 143 70 5 123 262 38 173 1.88e-24 96.7
MS.gene31637.t1 MTR_1g022105 44.068 118 55 7 80 195 168 276 4.23e-15 73.9
MS.gene31637.t1 MTR_7g090810 27.962 211 117 9 5 199 155 346 8.25e-13 68.2
MS.gene31637.t1 MTR_1g024125 40.336 119 61 6 130 244 38 150 1.10e-12 64.7
MS.gene31637.t1 MTR_7g090810 27.619 210 117 9 6 199 12 202 2.35e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 58 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATTCTAATCTCATTAAT+TGG 0.178899 1.1:+11593323 None:intergenic
GAAGATGATGAAGCATTTAT+TGG 0.198974 1.1:-11593746 MS.gene31637:CDS
GGTGTTTATCATTGGCTTAT+AGG 0.230766 1.1:-11593509 MS.gene31637:CDS
CAATTAATGAGATTAGAATT+TGG 0.234969 1.1:-11593322 MS.gene31637:CDS
ATGTACAATACTCTTGTTAA+TGG 0.263447 1.1:-11593530 MS.gene31637:CDS
TTGTTAGATTACATTGAAAT+TGG 0.271300 1.1:+11593129 None:intergenic
AATTAATGAGATTAGAATTT+GGG 0.326552 1.1:-11593321 MS.gene31637:CDS
ATATCTTCATTAGATCTTGT+TGG 0.350920 1.1:+11593177 None:intergenic
GATGAAGCATTTATTGGTTT+CGG 0.366469 1.1:-11593740 MS.gene31637:CDS
CATGATATCGCGGAGATTAA+CGG 0.370428 1.1:-11593386 MS.gene31637:CDS
GACAATGTCATAAGAAGGTT+GGG 0.376207 1.1:+11593775 None:intergenic
TTGTTAATGGTGTTTATCAT+TGG 0.389185 1.1:-11593517 MS.gene31637:CDS
ACTTTGAATTCGTTGGTGTT+GGG 0.418527 1.1:+11593710 None:intergenic
AAATCGTGATCATCATGGTT+GGG 0.422524 1.1:+11593485 None:intergenic
GGTTAAAGATCAACAAGGTT+GGG 0.438242 1.1:-11593300 MS.gene31637:CDS
ATCGTCCTTTACATTGGTCT+CGG 0.439450 1.1:-11593670 MS.gene31637:CDS
GTTGGACCTCTCTAGTGGTT+GGG 0.443076 1.1:+11593804 None:intergenic
GACTTTGAATTCGTTGGTGT+TGG 0.447818 1.1:+11593709 None:intergenic
ATTGAACGCATTCTTTCAAA+GGG 0.454335 1.1:-11593233 MS.gene31637:CDS
ATAGTTTCTGAAGGAGGAAC+AGG 0.455791 1.1:+11593581 None:intergenic
TATTGAACGCATTCTTTCAA+AGG 0.464379 1.1:-11593234 MS.gene31637:CDS
CTTGTTGGAACATTCTATCA+AGG 0.472063 1.1:+11593192 None:intergenic
CGACAATGTCATAAGAAGGT+TGG 0.488384 1.1:+11593774 None:intergenic
TCTGAAGGAGGAACAGGTAA+AGG 0.489798 1.1:+11593587 None:intergenic
AGGAACAGGTAAAGGGATAA+TGG 0.497272 1.1:+11593595 None:intergenic
TGGAAAGGACTATATCGAAC+TGG 0.498828 1.1:+11593410 None:intergenic
GGGTTAAAGATCAACAAGGT+TGG 0.505393 1.1:-11593301 MS.gene31637:CDS
AAGAAGGTTGGGGAAGAAGT+TGG 0.507507 1.1:+11593786 None:intergenic
AGTTGGACCTCTCTAGTGGT+TGG 0.517562 1.1:+11593803 None:intergenic
ATAATCATAGCACTAAATCA+TGG 0.517780 1.1:-11593619 MS.gene31637:CDS
CTGAAGGAGGAACAGGTAAA+GGG 0.518344 1.1:+11593588 None:intergenic
ACGCATTCTTTCAAAGGGGA+AGG 0.532718 1.1:-11593228 MS.gene31637:CDS
GTAATCTAACAAGTGATATG+TGG 0.542533 1.1:-11593118 MS.gene31637:CDS
TGTTAAAATCGTGATCATCA+TGG 0.545146 1.1:+11593480 None:intergenic
AAAATCGTGATCATCATGGT+TGG 0.549224 1.1:+11593484 None:intergenic
ATTTCGATTAACATAAGCAA+GGG 0.557921 1.1:+11593361 None:intergenic
GTATCCATCGTCCTTTACAT+TGG 0.559670 1.1:-11593676 MS.gene31637:CDS
AATCGTGATCATCATGGTTG+GGG 0.560502 1.1:+11593486 None:intergenic
TTTGACGACTTTGAATTCGT+TGG 0.564086 1.1:+11593703 None:intergenic
TCTACGACAATGTCATAAGA+AGG 0.569668 1.1:+11593770 None:intergenic
TAATCTCCGCGATATCATGA+TGG 0.585641 1.1:+11593390 None:intergenic
GTGTCGTAATTACGTTGACA+AGG 0.594721 1.1:+11593067 None:intergenic
CTAGACCGAGACCAATGTAA+AGG 0.611930 1.1:+11593665 None:intergenic
AATTTCGATTAACATAAGCA+AGG 0.613905 1.1:+11593360 None:intergenic
TCCGCGATATCATGATGGAA+AGG 0.614688 1.1:+11593395 None:intergenic
TCAGAAACTATCATATATCA+CGG 0.615345 1.1:-11593569 MS.gene31637:CDS
GTGGAACCCAACCACTAGAG+AGG 0.618329 1.1:-11593810 MS.gene31637:CDS
AAGAAGTTGGACCTCTCTAG+TGG 0.621541 1.1:+11593799 None:intergenic
TCCTTTCCATCATGATATCG+CGG 0.629162 1.1:-11593396 MS.gene31637:CDS
TGTCGTAATTACGTTGACAA+GGG 0.635110 1.1:+11593068 None:intergenic
TGATATATGATAGTTTCTGA+AGG 0.639815 1.1:+11593572 None:intergenic
ACAATGTCATAAGAAGGTTG+GGG 0.640082 1.1:+11593776 None:intergenic
TATATGATAGTTTCTGAAGG+AGG 0.646456 1.1:+11593575 None:intergenic
GTAGTTGAAACTGATTCGTG+TGG 0.653741 1.1:+11593441 None:intergenic
GAGACCAATGTAAAGGACGA+TGG 0.665459 1.1:+11593672 None:intergenic
ATTTGGGTTAAAGATCAACA+AGG 0.669261 1.1:-11593305 MS.gene31637:CDS
GAATGCGTTCAATATCAAGA+CGG 0.697697 1.1:+11593243 None:intergenic
TTGAACGCATTCTTTCAAAG+GGG 0.725587 1.1:-11593232 MS.gene31637:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATGAGATTAGAATTT+GGG - chr1.1:11593597-11593616 MS.gene31637:CDS 15.0%
!! CAAATTCTAATCTCATTAAT+TGG + chr1.1:11593598-11593617 None:intergenic 20.0%
!! CAATTAATGAGATTAGAATT+TGG - chr1.1:11593596-11593615 MS.gene31637:CDS 20.0%
!! TTGTTAGATTACATTGAAAT+TGG + chr1.1:11593792-11593811 None:intergenic 20.0%
! AATTTCGATTAACATAAGCA+AGG + chr1.1:11593561-11593580 None:intergenic 25.0%
! ATAATCATAGCACTAAATCA+TGG - chr1.1:11593299-11593318 MS.gene31637:CDS 25.0%
! ATATCTTCATTAGATCTTGT+TGG + chr1.1:11593744-11593763 None:intergenic 25.0%
! ATGTACAATACTCTTGTTAA+TGG - chr1.1:11593388-11593407 MS.gene31637:CDS 25.0%
! ATTTCGATTAACATAAGCAA+GGG + chr1.1:11593560-11593579 None:intergenic 25.0%
! TCAGAAACTATCATATATCA+CGG - chr1.1:11593349-11593368 MS.gene31637:CDS 25.0%
! TGATATATGATAGTTTCTGA+AGG + chr1.1:11593349-11593368 None:intergenic 25.0%
! TTGTTAATGGTGTTTATCAT+TGG - chr1.1:11593401-11593420 MS.gene31637:CDS 25.0%
ATTGAACGCATTCTTTCAAA+GGG - chr1.1:11593685-11593704 MS.gene31637:CDS 30.0%
GTAATCTAACAAGTGATATG+TGG - chr1.1:11593800-11593819 MS.gene31637:CDS 30.0%
TATTGAACGCATTCTTTCAA+AGG - chr1.1:11593684-11593703 MS.gene31637:CDS 30.0%
TGTTAAAATCGTGATCATCA+TGG + chr1.1:11593441-11593460 None:intergenic 30.0%
! ATTTGGGTTAAAGATCAACA+AGG - chr1.1:11593613-11593632 MS.gene31637:CDS 30.0%
! GAAGATGATGAAGCATTTAT+TGG - chr1.1:11593172-11593191 MS.gene31637:CDS 30.0%
! TATATGATAGTTTCTGAAGG+AGG + chr1.1:11593346-11593365 None:intergenic 30.0%
! TTCAAGACGACTTAATTTTG+TGG - chr1.1:11593089-11593108 MS.gene31637:CDS 30.0%
!! GATGAAGCATTTATTGGTTT+CGG - chr1.1:11593178-11593197 MS.gene31637:CDS 30.0%
AAAATCGTGATCATCATGGT+TGG + chr1.1:11593437-11593456 None:intergenic 35.0%
AAATCGTGATCATCATGGTT+GGG + chr1.1:11593436-11593455 None:intergenic 35.0%
ACAATGTCATAAGAAGGTTG+GGG + chr1.1:11593145-11593164 None:intergenic 35.0%
CTTGTTGGAACATTCTATCA+AGG + chr1.1:11593729-11593748 None:intergenic 35.0%
GAATGCGTTCAATATCAAGA+CGG + chr1.1:11593678-11593697 None:intergenic 35.0%
GACAATGTCATAAGAAGGTT+GGG + chr1.1:11593146-11593165 None:intergenic 35.0%
GGTTAAAGATCAACAAGGTT+GGG - chr1.1:11593618-11593637 MS.gene31637:CDS 35.0%
TCTACGACAATGTCATAAGA+AGG + chr1.1:11593151-11593170 None:intergenic 35.0%
TTGAACGCATTCTTTCAAAG+GGG - chr1.1:11593686-11593705 MS.gene31637:CDS 35.0%
TTTGACGACTTTGAATTCGT+TGG + chr1.1:11593218-11593237 None:intergenic 35.0%
! ACTTTGAATTCGTTGGTGTT+GGG + chr1.1:11593211-11593230 None:intergenic 35.0%
!! GGTGTTTATCATTGGCTTAT+AGG - chr1.1:11593409-11593428 MS.gene31637:CDS 35.0%
AATCGTGATCATCATGGTTG+GGG + chr1.1:11593435-11593454 None:intergenic 40.0%
AGGAACAGGTAAAGGGATAA+TGG + chr1.1:11593326-11593345 None:intergenic 40.0%
CATGATATCGCGGAGATTAA+CGG - chr1.1:11593532-11593551 MS.gene31637:CDS 40.0%
CGACAATGTCATAAGAAGGT+TGG + chr1.1:11593147-11593166 None:intergenic 40.0%
GACTTTGAATTCGTTGGTGT+TGG + chr1.1:11593212-11593231 None:intergenic 40.0%
GGGTTAAAGATCAACAAGGT+TGG - chr1.1:11593617-11593636 MS.gene31637:CDS 40.0%
GTATCCATCGTCCTTTACAT+TGG - chr1.1:11593242-11593261 MS.gene31637:CDS 40.0%
TAATCTCCGCGATATCATGA+TGG + chr1.1:11593531-11593550 None:intergenic 40.0%
TCCTTTCCATCATGATATCG+CGG - chr1.1:11593522-11593541 MS.gene31637:CDS 40.0%
TGGAAAGGACTATATCGAAC+TGG + chr1.1:11593511-11593530 None:intergenic 40.0%
! ATAGTTTCTGAAGGAGGAAC+AGG + chr1.1:11593340-11593359 None:intergenic 40.0%
! ATCGTCCTTTACATTGGTCT+CGG - chr1.1:11593248-11593267 MS.gene31637:CDS 40.0%
!! GTAGTTGAAACTGATTCGTG+TGG + chr1.1:11593480-11593499 None:intergenic 40.0%
AAGAAGGTTGGGGAAGAAGT+TGG + chr1.1:11593135-11593154 None:intergenic 45.0%
AAGAAGTTGGACCTCTCTAG+TGG + chr1.1:11593122-11593141 None:intergenic 45.0%
ACGCATTCTTTCAAAGGGGA+AGG - chr1.1:11593690-11593709 MS.gene31637:CDS 45.0%
CTAGACCGAGACCAATGTAA+AGG + chr1.1:11593256-11593275 None:intergenic 45.0%
CTGAAGGAGGAACAGGTAAA+GGG + chr1.1:11593333-11593352 None:intergenic 45.0%
GAGACCAATGTAAAGGACGA+TGG + chr1.1:11593249-11593268 None:intergenic 45.0%
TCTGAAGGAGGAACAGGTAA+AGG + chr1.1:11593334-11593353 None:intergenic 45.0%
! TCCGCGATATCATGATGGAA+AGG + chr1.1:11593526-11593545 None:intergenic 45.0%
AGTTGGACCTCTCTAGTGGT+TGG + chr1.1:11593118-11593137 None:intergenic 50.0%
GTTGGACCTCTCTAGTGGTT+GGG + chr1.1:11593117-11593136 None:intergenic 50.0%
GTGGAACCCAACCACTAGAG+AGG - chr1.1:11593108-11593127 MS.gene31637:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 11593076 11593864 11593076 ID=MS.gene31637
chr1.1 mRNA 11593076 11593864 11593076 ID=MS.gene31637.t1;Parent=MS.gene31637
chr1.1 exon 11593076 11593864 11593076 ID=MS.gene31637.t1.exon1;Parent=MS.gene31637.t1
chr1.1 CDS 11593076 11593864 11593076 ID=cds.MS.gene31637.t1;Parent=MS.gene31637.t1
Gene Sequence

>MS.gene31637

ATGGAGAATCAAATTCAAGACGACTTAATTTTGTGGAACCCAACCACTAGAGAGGTCCAACTTCTTCCCCAACCTTCTTATGACATTGTCGTAGATGAAGATGATGAAGCATTTATTGGTTTCGGTGCTGATCCCAACACCAACGAATTCAAAGTCGTCAAAGTCAGTATCCATCGTCCTTTACATTGGTCTCGGTCTAGTTCTTTTTCACTTGCTGAACTTTATAATCATAGCACTAAATCATGGACCATTATCCCTTTACCTGTTCCTCCTTCAGAAACTATCATATATCACGGTCATAGACGTTTTAACATGTACAATACTCTTGTTAATGGTGTTTATCATTGGCTTATAGGCCCCAACCATGATGATCACGATTTTAACATTCTCTGCTTTGACTTCCACACGAATCAGTTTCAACTACTCGAGTCTCCAGTTCGATATAGTCCTTTCCATCATGATATCGCGGAGATTAACGGTTCCCTTGCTTATGTTAATCGAAATTTTTATGCTTTTAATCCAATTAATGAGATTAGAATTTGGGTTAAAGATCAACAAGGTTGGGTCAAGAAATACAACATTAATATCTCACTTGATCGCCGTCTTGATATTGAACGCATTCTTTCAAAGGGGAAGGATGATGTTCAAGTCCTTGATAGAATGTTCCAACAAGATCTAATGAAGATATACGATAAAGATTGCAACTTGCTTCACCAATTTCAATGTAATCTAACAAGTGATATGTGGATTCATGAGTATGTGCAAAGTATAGTTCCCTTGTCAACGTAA

Protein sequence

>MS.gene31637.t1

MENQIQDDLILWNPTTREVQLLPQPSYDIVVDEDDEAFIGFGADPNTNEFKVVKVSIHRPLHWSRSSSFSLAELYNHSTKSWTIIPLPVPPSETIIYHGHRRFNMYNTLVNGVYHWLIGPNHDDHDFNILCFDFHTNQFQLLESPVRYSPFHHDIAEINGSLAYVNRNFYAFNPINEIRIWVKDQQGWVKKYNINISLDRRLDIERILSKGKDDVQVLDRMFQQDLMKIYDKDCNLLHQFQCNLTSDMWIHEYVQSIVPLST