AlfalfaGEDB | MS.gene27051

M. sativa cultivar XinJiangDaYe / MS.gene27051

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27051.t1	RHN77539.1	76.2	239	54	2	1	239	146	381	1.10E-100	376.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27051.t1	G7I5X5	76.2	239	54	2	1	239	117	352	7.7e-101	376.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene27051	MS.gene066120	PPI
MS.gene50309	MS.gene27051	PPI
MS.gene036760	MS.gene27051	PPI
MS.gene27051	MS.gene035235	PPI
MS.gene58311	MS.gene27051	PPI
MS.gene27051	MS.gene058703	PPI
MS.gene57527	MS.gene27051	PPI
MS.gene033432	MS.gene27051	PPI
MS.gene27051	MS.gene024000	PPI
MS.gene27051	MS.gene012215	PPI
MS.gene033426	MS.gene27051	PPI
MS.gene59639	MS.gene27051	PPI
MS.gene27051	MS.gene033079	PPI
MS.gene67687	MS.gene27051	PPI
MS.gene27051	MS.gene055694	PPI
MS.gene27051	MS.gene059101	PPI
MS.gene035261	MS.gene27051	PPI
MS.gene27051	MS.gene58316	PPI
MS.gene063670	MS.gene27051	PPI
MS.gene27051	MS.gene58321	PPI
MS.gene57530	MS.gene27051	PPI
MS.gene27051	MS.gene87564	PPI
MS.gene27051	MS.gene58315	PPI
MS.gene019506	MS.gene27051	PPI
MS.gene50326	MS.gene27051	PPI
MS.gene69309	MS.gene27051	PPI
MS.gene024002	MS.gene27051	PPI
MS.gene27051	MS.gene041473	PPI
MS.gene27051	MS.gene50311	PPI
MS.gene88774	MS.gene27051	PPI
MS.gene27051	MS.gene058989	PPI
MS.gene27051	MS.gene036556	PPI
MS.gene034686	MS.gene27051	PPI
MS.gene27051	MS.gene57538	PPI
MS.gene40982	MS.gene27051	PPI
MS.gene070461	MS.gene27051	PPI
MS.gene42452	MS.gene27051	PPI
MS.gene27051	MS.gene058693	PPI
MS.gene27051	MS.gene27319	PPI
MS.gene27051	MS.gene041395	PPI
MS.gene27051	MS.gene51189	PPI
MS.gene036662	MS.gene27051	PPI
MS.gene27051	MS.gene046958	PPI
MS.gene87563	MS.gene27051	PPI
MS.gene57526	MS.gene27051	PPI
MS.gene27051	MS.gene24286	PPI
MS.gene051820	MS.gene27051	PPI
MS.gene97996	MS.gene27051	PPI
MS.gene27051	MS.gene58783	PPI
MS.gene81242	MS.gene27051	PPI
MS.gene27051	MS.gene40988	PPI
MS.gene26101	MS.gene27051	PPI
MS.gene27283	MS.gene27051	PPI
MS.gene64203	MS.gene27051	PPI
MS.gene27051	MS.gene24173	PPI
MS.gene045140	MS.gene27051	PPI
MS.gene27051	MS.gene036584	PPI
MS.gene27051	MS.gene036662	PPI
MS.gene024004	MS.gene27051	PPI
MS.gene42444	MS.gene27051	PPI
MS.gene51202	MS.gene27051	PPI
MS.gene27051	MS.gene42451	PPI
MS.gene87562	MS.gene27051	PPI
MS.gene27051	MS.gene035261	PPI
MS.gene27051	MS.gene023999	PPI
MS.gene27051	MS.gene69307	PPI
MS.gene064104	MS.gene27051	PPI
MS.gene27051	MS.gene036581	PPI
MS.gene041491	MS.gene27051	PPI
MS.gene27051	MS.gene019507	PPI
MS.gene27051	MS.gene033431	PPI
MS.gene033443	MS.gene27051	PPI
MS.gene43789	MS.gene27051	PPI
MS.gene059096	MS.gene27051	PPI
MS.gene57528	MS.gene27051	PPI
MS.gene27051	MS.gene50320	PPI
MS.gene27051	MS.gene58314	PPI
MS.gene27051	MS.gene87557	PPI
MS.gene006711	MS.gene27051	PPI
MS.gene27051	MS.gene035263	PPI
MS.gene27285	MS.gene27051	PPI
MS.gene072177	MS.gene27051	PPI
MS.gene27051	MS.gene67501	PPI
MS.gene064091	MS.gene27051	PPI
MS.gene27051	MS.gene96406	PPI
MS.gene064112	MS.gene27051	PPI
MS.gene27051	MS.gene035262	PPI
MS.gene045476	MS.gene27051	PPI
MS.gene24208	MS.gene27051	PPI
MS.gene50307	MS.gene27051	PPI
MS.gene27051	MS.gene028390	PPI
MS.gene43796	MS.gene27051	PPI
MS.gene034690	MS.gene27051	PPI
MS.gene27051	MS.gene40878	PPI
MS.gene58312	MS.gene27051	PPI
MS.gene96407	MS.gene27051	PPI
MS.gene77857	MS.gene27051	PPI
MS.gene51191	MS.gene27051	PPI
MS.gene27051	MS.gene047279	PPI
MS.gene27051	MS.gene50305	PPI
MS.gene27051	MS.gene032969	PPI
MS.gene27051	MS.gene68890	PPI
MS.gene033430	MS.gene27051	PPI
MS.gene50298	MS.gene27051	PPI
MS.gene27051	MS.gene42445	PPI
MS.gene27051	MS.gene86273	PPI
MS.gene27051	MS.gene26830	PPI
MS.gene036583	MS.gene27051	PPI
MS.gene27051	MS.gene22715	PPI
MS.gene88780	MS.gene27051	PPI
MS.gene27051	MS.gene51207	PPI
MS.gene27051	MS.gene63657	PPI
MS.gene27051	MS.gene059157	PPI
MS.gene27051	MS.gene36595	PPI
MS.gene71159	MS.gene27051	PPI
MS.gene96448	MS.gene27051	PPI
MS.gene27051	MS.gene024004	PPI
MS.gene77858	MS.gene27051	PPI
MS.gene058692	MS.gene27051	PPI
MS.gene041392	MS.gene27051	PPI
MS.gene27051	MS.gene070462	PPI
MS.gene27051	MS.gene061240	PPI
MS.gene033083	MS.gene27051	PPI
MS.gene63658	MS.gene27051	PPI
MS.gene033081	MS.gene27051	PPI
MS.gene031591	MS.gene27051	PPI
MS.gene27051	MS.gene064086	PPI
MS.gene27051	MS.gene035234	PPI
MS.gene035262	MS.gene27051	PPI
MS.gene27051	MS.gene041468	PPI
MS.gene51199	MS.gene27051	PPI
MS.gene57536	MS.gene27051	PPI
MS.gene019503	MS.gene27051	PPI
MS.gene27051	MS.gene50332	PPI
MS.gene27051	MS.gene42447	PPI
MS.gene27051	MS.gene024979	PPI
MS.gene87559	MS.gene27051	PPI
MS.gene033431	MS.gene27051	PPI
MS.gene27051	MS.gene24826	PPI
MS.gene27051	MS.gene044425	PPI
MS.gene041475	MS.gene27051	PPI
MS.gene31587	MS.gene27051	PPI
MS.gene033441	MS.gene27051	PPI
MS.gene27051	MS.gene59637	PPI
MS.gene063826	MS.gene27051	PPI
MS.gene060367	MS.gene27051	PPI
MS.gene27051	MS.gene27022	PPI
MS.gene44892	MS.gene27051	PPI
MS.gene58239	MS.gene27051	PPI
MS.gene27051	MS.gene87585	PPI
MS.gene27051	MS.gene024009	PPI
MS.gene000038	MS.gene27051	PPI
MS.gene27051	MS.gene036599	PPI
MS.gene57531	MS.gene27051	PPI
MS.gene064111	MS.gene27051	PPI
MS.gene26829	MS.gene27051	PPI
MS.gene39262	MS.gene27051	PPI
MS.gene27051	MS.gene006705	PPI
MS.gene053256	MS.gene27051	PPI
MS.gene27051	MS.gene035237	PPI
MS.gene75875	MS.gene27051	PPI
MS.gene041470	MS.gene27051	PPI
MS.gene058694	MS.gene27051	PPI
MS.gene54049	MS.gene27051	PPI
MS.gene27051	MS.gene000036	PPI
MS.gene27051	MS.gene50309	PPI
MS.gene27051	MS.gene041394	PPI
MS.gene006710	MS.gene27051	PPI
MS.gene58722	MS.gene27051	PPI
MS.gene27051	MS.gene036664	PPI
MS.gene27051	MS.gene053257	PPI
MS.gene27051	MS.gene58241	PPI
MS.gene27051	MS.gene77858	PPI
MS.gene27051	MS.gene51201	PPI
MS.gene27051	MS.gene058696	PPI
MS.gene64001	MS.gene27051	PPI
MS.gene27051	MS.gene009893	PPI
MS.gene27051	MS.gene50336	PPI
MS.gene57533	MS.gene27051	PPI
MS.gene36704	MS.gene27051	PPI
MS.gene032970	MS.gene27051	PPI
MS.gene27051	MS.gene77704	PPI
MS.gene77856	MS.gene27051	PPI
MS.gene58313	MS.gene27051	PPI
MS.gene27051	MS.gene033083	PPI
MS.gene066622	MS.gene27051	PPI
MS.gene44887	MS.gene27051	PPI
MS.gene85043	MS.gene27051	PPI
MS.gene63655	MS.gene27051	PPI
MS.gene44883	MS.gene27051	PPI
MS.gene27051	MS.gene036663	PPI
MS.gene27051	MS.gene68580	PPI
MS.gene41046	MS.gene27051	PPI
MS.gene87588	MS.gene27051	PPI
MS.gene017412	MS.gene27051	PPI
MS.gene27051	MS.gene57525	PPI
MS.gene80884	MS.gene27051	PPI
MS.gene27051	MS.gene023996	PPI
MS.gene27051	MS.gene030114	PPI
MS.gene27051	MS.gene045473	PPI
MS.gene51192	MS.gene27051	PPI
MS.gene009883	MS.gene27051	PPI
MS.gene27051	MS.gene50301	PPI
MS.gene27051	MS.gene045474	PPI
MS.gene58317	MS.gene27051	PPI
MS.gene27051	MS.gene031590	PPI
MS.gene27051	MS.gene036558	PPI
MS.gene044563	MS.gene27051	PPI
MS.gene069075	MS.gene27051	PPI
MS.gene033427	MS.gene27051	PPI
MS.gene27051	MS.gene40984	PPI
MS.gene40985	MS.gene27051	PPI
MS.gene27051	MS.gene066623	PPI
MS.gene27051	MS.gene064108	PPI
MS.gene041474	MS.gene27051	PPI
MS.gene27020	MS.gene27051	PPI
MS.gene27051	MS.gene064090	PPI
MS.gene27051	MS.gene58317	PPI
MS.gene041388	MS.gene27051	PPI
MS.gene27051	MS.gene90742	PPI
MS.gene062698	MS.gene27051	PPI
MS.gene27051	MS.gene24212	PPI
MS.gene96126	MS.gene27051	PPI
MS.gene51208	MS.gene27051	PPI
MS.gene016524	MS.gene27051	PPI
MS.gene50304	MS.gene27051	PPI
MS.gene27051	MS.gene58240	PPI
MS.gene045475	MS.gene27051	PPI
MS.gene88776	MS.gene27051	PPI
MS.gene59943	MS.gene27051	PPI
MS.gene27051	MS.gene063472	PPI
MS.gene90744	MS.gene27051	PPI
MS.gene27051	MS.gene43790	PPI
MS.gene51204	MS.gene27051	PPI
MS.gene27051	MS.gene57717	PPI
MS.gene27051	MS.gene034680	PPI
MS.gene036557	MS.gene27051	PPI
MS.gene041393	MS.gene27051	PPI
MS.gene50329	MS.gene27051	PPI
MS.gene27051	MS.gene50327	PPI
MS.gene27051	MS.gene066626	PPI
MS.gene57537	MS.gene27051	PPI
MS.gene27051	MS.gene063474	PPI
MS.gene059156	MS.gene27051	PPI
MS.gene84698	MS.gene27051	PPI
MS.gene27051	MS.gene88416	PPI
MS.gene27051	MS.gene57530	PPI
MS.gene27051	MS.gene034681	PPI
MS.gene009891	MS.gene27051	PPI
MS.gene27051	MS.gene57532	PPI
MS.gene27051	MS.gene003076	PPI
MS.gene066641	MS.gene27051	PPI
MS.gene82082	MS.gene27051	PPI
MS.gene27051	MS.gene009892	PPI
MS.gene27051	MS.gene061241	PPI
MS.gene57535	MS.gene27051	PPI
MS.gene27051	MS.gene058669	PPI
MS.gene025887	MS.gene27051	PPI
MS.gene27051	MS.gene51188	PPI
MS.gene87200	MS.gene27051	PPI
MS.gene27051	MS.gene041489	PPI
MS.gene27051	MS.gene38220	PPI
MS.gene27051	MS.gene40986	PPI
MS.gene27051	MS.gene034688	PPI
MS.gene015089	MS.gene27051	PPI
MS.gene27051	MS.gene03060	PPI
MS.gene27051	MS.gene24213	PPI
MS.gene27051	MS.gene033440	PPI
MS.gene27051	MS.gene024011	PPI
MS.gene44885	MS.gene27051	PPI
MS.gene011679	MS.gene27051	PPI
MS.gene27051	MS.gene034677	PPI
MS.gene060370	MS.gene27051	PPI
MS.gene27051	MS.gene064109	PPI
MS.gene50327	MS.gene27051	PPI
MS.gene066635	MS.gene27051	PPI
MS.gene27051	MS.gene035238	PPI
MS.gene27051	MS.gene85043	PPI
MS.gene27051	MS.gene064114	PPI
MS.gene010758	MS.gene27051	PPI
MS.gene27051	MS.gene87584	PPI
MS.gene87586	MS.gene27051	PPI
MS.gene27051	MS.gene80610	PPI
MS.gene51200	MS.gene27051	PPI
MS.gene40887	MS.gene27051	PPI
MS.gene50339	MS.gene27051	PPI
MS.gene046958	MS.gene27051	PPI
MS.gene27051	MS.gene90743	PPI
MS.gene27051	MS.gene40886	PPI
MS.gene060366	MS.gene27051	PPI
MS.gene27051	MS.gene85454	PPI
MS.gene27051	MS.gene041391	PPI
MS.gene27051	MS.gene033443	PPI
MS.gene57529	MS.gene27051	PPI
MS.gene063414	MS.gene27051	PPI
MS.gene40902	MS.gene27051	PPI
MS.gene27051	MS.gene058701	PPI
MS.gene27051	MS.gene87554	PPI
MS.gene27051	MS.gene009891	PPI
MS.gene27051	MS.gene064083	PPI
MS.gene021553	MS.gene27051	PPI
MS.gene26957	MS.gene27051	PPI
MS.gene060368	MS.gene27051	PPI
MS.gene033875	MS.gene27051	PPI
MS.gene27051	MS.gene048193	PPI
MS.gene036582	MS.gene27051	PPI
MS.gene27021	MS.gene27051	PPI
MS.gene27051	MS.gene024420	PPI
MS.gene066620	MS.gene27051	PPI
MS.gene27051	MS.gene016220	PPI
MS.gene035236	MS.gene27051	PPI
MS.gene58320	MS.gene27051	PPI
MS.gene27051	MS.gene024008	PPI
MS.gene27051	MS.gene50337	PPI
MS.gene27051	MS.gene54541	PPI
MS.gene27051	MS.gene39265	PPI
MS.gene27051	MS.gene060368	PPI
MS.gene58781	MS.gene27051	PPI
MS.gene27051	MS.gene053299	PPI
MS.gene028390	MS.gene27051	PPI
MS.gene011106	MS.gene27051	PPI
MS.gene033440	MS.gene27051	PPI
MS.gene27051	MS.gene28758	PPI
MS.gene62398	MS.gene27051	PPI
MS.gene27051	MS.gene044429	PPI
MS.gene60027	MS.gene27051	PPI
MS.gene44886	MS.gene27051	PPI
MS.gene038756	MS.gene27051	PPI
MS.gene40904	MS.gene27051	PPI
MS.gene27051	MS.gene27284	PPI
MS.gene35068	MS.gene27051	PPI
MS.gene041390	MS.gene27051	PPI
MS.gene24286	MS.gene27051	PPI
MS.gene92376	MS.gene27051	PPI
MS.gene000035	MS.gene27051	PPI
MS.gene27051	MS.gene48221	PPI
MS.gene87569	MS.gene27051	PPI
MS.gene31637	MS.gene27051	PPI
MS.gene87573	MS.gene27051	PPI
MS.gene019504	MS.gene27051	PPI
MS.gene034762	MS.gene27051	PPI
MS.gene27051	MS.gene51190	PPI
MS.gene27051	MS.gene58319	PPI
MS.gene27051	MS.gene034762	PPI
MS.gene27051	MS.gene59943	PPI
MS.gene27051	MS.gene044426	PPI
MS.gene50297	MS.gene27051	PPI
MS.gene58716	MS.gene27051	PPI
MS.gene27051	MS.gene033442	PPI
MS.gene033444	MS.gene27051	PPI
MS.gene019505	MS.gene27051	PPI
MS.gene50306	MS.gene27051	PPI
MS.gene58785	MS.gene27051	PPI
MS.gene066639	MS.gene27051	PPI
MS.gene26949	MS.gene27051	PPI
MS.gene27051	MS.gene44428	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27051.t1	MTR_1g023400	76.151	239	54	2	1	239	117	352	5.19e-129	369
MS.gene27051.t1	MTR_1g022200	64.591	257	76	7	3	247	127	380	3.05e-105	310
MS.gene27051.t1	MTR_1g024125	92.373	118	8	1	110	226	38	155	3.63e-78	233
MS.gene27051.t1	MTR_1g023430	69.677	155	43	2	94	247	29	180	3.92e-72	218
MS.gene27051.t1	MTR_4g080200	48.400	250	109	8	1	239	109	349	2.43e-64	204
MS.gene27051.t1	MTR_1g009110	46.850	254	110	9	1	240	137	379	1.59e-63	203
MS.gene27051.t1	MTR_1g037540	43.893	262	115	12	1	239	148	400	4.98e-48	163
MS.gene27051.t1	MTR_1g022105	49.375	160	73	3	62	221	169	320	7.69e-44	150
MS.gene27051.t1	MTR_1g008000	35.918	245	106	11	7	240	65	269	2.01e-25	101
MS.gene27051.t1	MTR_5g027330	28.959	221	127	12	1	214	173	370	7.69e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 70 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGATGATGTTGCCGAGATTA+AGG	0.171278	1.1:-825491	MS.gene27051:CDS
CTCCTGTCGTTTCGAACATT+TGG	0.229411	1.1:-825432	MS.gene27051:CDS
TATGAACATCAATTTGAAAT+TGG	0.244562	1.1:+825254	None:intergenic
AACTCACGCTTGCATGGTTT+TGG	0.294349	1.1:-825838	MS.gene27051:CDS
TAAGACTAAATATTGGATTC+AGG	0.310121	1.1:-825233	MS.gene27051:CDS
GAACTCCTTGGAGGAAAGTT+TGG	0.311876	1.1:-825319	MS.gene27051:CDS
ATATAAGTTATGATGGAATT+AGG	0.312979	1.1:-825771	MS.gene27051:CDS
GTTTGGTGTCCTAGCTATCT+TGG	0.313901	1.1:+825180	None:intergenic
TCACTCTACTATAGCAATAA+TGG	0.344231	1.1:-825607	MS.gene27051:CDS
ATGTTCATAAGACTAAATAT+TGG	0.349506	1.1:-825240	MS.gene27051:CDS
CAAATATATTGATCCTAGTT+TGG	0.355534	1.1:+825163	None:intergenic
AGGCGTCTGATCTTGGTAAA+AGG	0.359916	1.1:+825688	None:intergenic
GATGATGTTGCCGAGATTAA+GGG	0.361061	1.1:-825490	MS.gene27051:CDS
AGATATAACACTCTTGTTAA+CGG	0.387900	1.1:-825655	MS.gene27051:CDS
TCCTGTCGTTTCGAACATTT+GGG	0.389526	1.1:-825431	MS.gene27051:CDS
CGTTTCGAACATTTGGGTAA+TGG	0.394618	1.1:-825425	MS.gene27051:CDS
TTGTTAACGGCGTTTATCAT+TGG	0.397348	1.1:-825642	MS.gene27051:CDS
TGTCTCGTATGCTCGGCCTT+TGG	0.408788	1.1:-825354	MS.gene27051:CDS
GGCTAAGTGAGTTGCCTTGA+TGG	0.411411	1.1:+825275	None:intergenic
AATCAGTTTCACGAACTTAG+AGG	0.414322	1.1:-825550	MS.gene27051:CDS
ATAATCTAAGTACCAACTCT+TGG	0.426515	1.1:-825732	MS.gene27051:CDS
CGGTGGACCTCTCTAGTTGT+TGG	0.437420	1.1:+825889	None:intergenic
AGGACACAGGCGTCTGATCT+TGG	0.439056	1.1:+825681	None:intergenic
GATTCTGTGTAACATAAGTA+AGG	0.458247	1.1:+825464	None:intergenic
ACAAGAGTGTTATATCTAGA+AGG	0.467432	1.1:+825661	None:intergenic
AAACTCTATATAAGTTATGA+TGG	0.468261	1.1:-825778	MS.gene27051:CDS
GACAACAACTCACGCTTGCA+TGG	0.471974	1.1:-825844	MS.gene27051:CDS
GGTGGACCTCTCTAGTTGTT+GGG	0.479668	1.1:+825890	None:intergenic
AAGGAGGAGGATGTTCAGTT+GGG	0.484768	1.1:+825707	None:intergenic
CCTAGCTATCTTGGTTGACA+AGG	0.485062	1.1:+825189	None:intergenic
ATGCTCGGCCTTTGGAAGAA+TGG	0.492025	1.1:-825346	MS.gene27051:CDS
AACTTTGAAGCTGATGTTGT+TGG	0.493018	1.1:+825804	None:intergenic
AAAGGAGGAGGATGTTCAGT+TGG	0.498063	1.1:+825706	None:intergenic
ACTTTGAAGCTGATGTTGTT+GGG	0.507643	1.1:+825805	None:intergenic
GTATCAATGTCTCGTATGCT+CGG	0.516361	1.1:-825361	MS.gene27051:CDS
AAGAATGGCGATGAACTCCT+TGG	0.520103	1.1:-825331	MS.gene27051:CDS
CTAGCTATCTTGGTTGACAA+GGG	0.528799	1.1:+825190	None:intergenic
CCTTGTCAACCAAGATAGCT+AGG	0.532417	1.1:-825189	MS.gene27051:CDS
AGCATACGAGACATTGATAC+AGG	0.533749	1.1:+825364	None:intergenic
TTCAGTTGGGGTCCAAGAGT+TGG	0.536792	1.1:+825720	None:intergenic
GATAGCTAGGACACCAAACT+AGG	0.547828	1.1:-825176	None:intergenic
AGTAAGGGAACCCTTAATCT+CGG	0.554521	1.1:+825480	None:intergenic
CTGATCTTGGTAAAAGGAGG+AGG	0.555515	1.1:+825694	None:intergenic
ATCAGTTTCACGAACTTAGA+GGG	0.563965	1.1:-825549	MS.gene27051:CDS
GTTCATCGCCATTCTTCCAA+AGG	0.565148	1.1:+825338	None:intergenic
ACCCAAATGTTCGAAACGAC+AGG	0.568868	1.1:+825430	None:intergenic
AATGGCGATGAACTCCTTGG+AGG	0.569060	1.1:-825328	MS.gene27051:CDS
GTTCTCCAAACTTTCCTCCA+AGG	0.571961	1.1:+825314	None:intergenic
GTTGCGGTCTTCAACTGAGA+AGG	0.574764	1.1:+825522	None:intergenic
CTCAAATCATATGACCATCA+AGG	0.583706	1.1:-825289	MS.gene27051:CDS
CGGTCTTCAACTGAGAAGGT+TGG	0.586128	1.1:+825526	None:intergenic
TAGCTATCTTGGTTGACAAG+GGG	0.591109	1.1:+825191	None:intergenic
AGCTATCTTGGTTGACAAGG+GGG	0.593652	1.1:+825192	None:intergenic
TGATGGTCATATGATTTGAG+CGG	0.609744	1.1:+825292	None:intergenic
ATTCTGTGTAACATAAGTAA+GGG	0.613076	1.1:+825465	None:intergenic
TTGTTGTCAAGGCAGAGACT+CGG	0.617430	1.1:+825859	None:intergenic
GGGTAATGGATCAAAGTGGA+TGG	0.622462	1.1:-825411	MS.gene27051:CDS
GGCAGAGACTCGGAGGAACA+CGG	0.631574	1.1:+825869	None:intergenic
CGTCTGATCTTGGTAAAAGG+AGG	0.635316	1.1:+825691	None:intergenic
GCAAGCGTGAGTTGTTGTCA+AGG	0.635753	1.1:+825848	None:intergenic
TACGAGACATTGATACAGGT+GGG	0.641469	1.1:+825368	None:intergenic
TGTTATATCTAGAAGGACAC+AGG	0.641742	1.1:+825668	None:intergenic
ATACGAGACATTGATACAGG+TGG	0.649476	1.1:+825367	None:intergenic
ATTTGGGTAATGGATCAAAG+TGG	0.662912	1.1:-825415	MS.gene27051:CDS
CATCATCGCGTAGAAAGTTG+CGG	0.667366	1.1:+825506	None:intergenic
GTGGAACCCAACAACTAGAG+AGG	0.673963	1.1:-825896	MS.gene27051:CDS
AGGAGGAGGATGTTCAGTTG+GGG	0.674344	1.1:+825708	None:intergenic
TGATGGAATTAGGAGATCAG+CGG	0.680423	1.1:-825761	MS.gene27051:CDS
TTGTCAAGGCAGAGACTCGG+AGG	0.701974	1.1:+825862	None:intergenic
AGAGACTCGGAGGAACACGG+TGG	0.812172	1.1:+825872	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAACTCTATATAAGTTATGA+TGG	-	chr1.1:825297-825316	MS.gene27051:CDS	20.0%
!!	ATGTTCATAAGACTAAATAT+TGG	-	chr1.1:825835-825854	MS.gene27051:CDS	20.0%
!!!	ATATAAGTTATGATGGAATT+AGG	-	chr1.1:825304-825323	MS.gene27051:CDS	20.0%
!!!	TATGAACATCAATTTGAAAT+TGG	+	chr1.1:825824-825843	None:intergenic	20.0%
!	AGATATAACACTCTTGTTAA+CGG	-	chr1.1:825420-825439	MS.gene27051:CDS	25.0%
!	ATTCTGTGTAACATAAGTAA+GGG	+	chr1.1:825613-825632	None:intergenic	25.0%
!	TAAGACTAAATATTGGATTC+AGG	-	chr1.1:825842-825861	MS.gene27051:CDS	25.0%
	ATAATCTAAGTACCAACTCT+TGG	-	chr1.1:825343-825362	MS.gene27051:CDS	30.0%
	GATTCTGTGTAACATAAGTA+AGG	+	chr1.1:825614-825633	None:intergenic	30.0%
	TCACTCTACTATAGCAATAA+TGG	-	chr1.1:825468-825487	MS.gene27051:CDS	30.0%
!!	ACAAGAGTGTTATATCTAGA+AGG	+	chr1.1:825417-825436	None:intergenic	30.0%
	AATCAGTTTCACGAACTTAG+AGG	-	chr1.1:825525-825544	MS.gene27051:CDS	35.0%
	ATCAGTTTCACGAACTTAGA+GGG	-	chr1.1:825526-825545	MS.gene27051:CDS	35.0%
	CTCAAATCATATGACCATCA+AGG	-	chr1.1:825786-825805	MS.gene27051:CDS	35.0%
	TGATGGTCATATGATTTGAG+CGG	+	chr1.1:825786-825805	None:intergenic	35.0%
	TGTTATATCTAGAAGGACAC+AGG	+	chr1.1:825410-825429	None:intergenic	35.0%
	TTGTTAACGGCGTTTATCAT+TGG	-	chr1.1:825433-825452	MS.gene27051:CDS	35.0%
!	TGGCACAAAAAGTACAACAT+TGG	-	chr1.1:825684-825703	MS.gene27051:CDS	35.0%
!!	AACTTTGAAGCTGATGTTGT+TGG	+	chr1.1:825274-825293	None:intergenic	35.0%
!!	ACTTTGAAGCTGATGTTGTT+GGG	+	chr1.1:825273-825292	None:intergenic	35.0%
!!	ATTTGGGTAATGGATCAAAG+TGG	-	chr1.1:825660-825679	MS.gene27051:CDS	35.0%
	AGCATACGAGACATTGATAC+AGG	+	chr1.1:825714-825733	None:intergenic	40.0%
	AGTAAGGGAACCCTTAATCT+CGG	+	chr1.1:825598-825617	None:intergenic	40.0%
	ATACGAGACATTGATACAGG+TGG	+	chr1.1:825711-825730	None:intergenic	40.0%
	CGTTTCGAACATTTGGGTAA+TGG	-	chr1.1:825650-825669	MS.gene27051:CDS	40.0%
	CTAGCTATCTTGGTTGACAA+GGG	+	chr1.1:825888-825907	None:intergenic	40.0%
	GATGATGTTGCCGAGATTAA+GGG	-	chr1.1:825585-825604	MS.gene27051:CDS	40.0%
	GTATCAATGTCTCGTATGCT+CGG	-	chr1.1:825714-825733	MS.gene27051:CDS	40.0%
	TACGAGACATTGATACAGGT+GGG	+	chr1.1:825710-825729	None:intergenic	40.0%
	TAGCTATCTTGGTTGACAAG+GGG	+	chr1.1:825887-825906	None:intergenic	40.0%
	TGATGGAATTAGGAGATCAG+CGG	-	chr1.1:825314-825333	MS.gene27051:CDS	40.0%
!	TCCTGTCGTTTCGAACATTT+GGG	-	chr1.1:825644-825663	MS.gene27051:CDS	40.0%
	AAAGGAGGAGGATGTTCAGT+TGG	+	chr1.1:825372-825391	None:intergenic	45.0%
	AAGAATGGCGATGAACTCCT+TGG	-	chr1.1:825744-825763	MS.gene27051:CDS	45.0%
	AAGGAGGAGGATGTTCAGTT+GGG	+	chr1.1:825371-825390	None:intergenic	45.0%
	ACCCAAATGTTCGAAACGAC+AGG	+	chr1.1:825648-825667	None:intergenic	45.0%
	AGCTATCTTGGTTGACAAGG+GGG	+	chr1.1:825886-825905	None:intergenic	45.0%
	AGGCGTCTGATCTTGGTAAA+AGG	+	chr1.1:825390-825409	None:intergenic	45.0%
	CATCATCGCGTAGAAAGTTG+CGG	+	chr1.1:825572-825591	None:intergenic	45.0%
	CCTAGCTATCTTGGTTGACA+AGG	+	chr1.1:825889-825908	None:intergenic	45.0%
	CCTTGTCAACCAAGATAGCT+AGG	-	chr1.1:825886-825905	MS.gene27051:CDS	45.0%
	CGATGATGTTGCCGAGATTA+AGG	-	chr1.1:825584-825603	MS.gene27051:CDS	45.0%
	CGTCTGATCTTGGTAAAAGG+AGG	+	chr1.1:825387-825406	None:intergenic	45.0%
	CTGATCTTGGTAAAAGGAGG+AGG	+	chr1.1:825384-825403	None:intergenic	45.0%
	GAACTCCTTGGAGGAAAGTT+TGG	-	chr1.1:825756-825775	MS.gene27051:CDS	45.0%
	GTTCATCGCCATTCTTCCAA+AGG	+	chr1.1:825740-825759	None:intergenic	45.0%
	GTTCTCCAAACTTTCCTCCA+AGG	+	chr1.1:825764-825783	None:intergenic	45.0%
	TTGTTGTCAAGGCAGAGACT+CGG	+	chr1.1:825219-825238	None:intergenic	45.0%
!	AACTCACGCTTGCATGGTTT+TGG	-	chr1.1:825237-825256	MS.gene27051:CDS	45.0%
!	CTCCTGTCGTTTCGAACATT+TGG	-	chr1.1:825643-825662	MS.gene27051:CDS	45.0%
!!	GGGTAATGGATCAAAGTGGA+TGG	-	chr1.1:825664-825683	MS.gene27051:CDS	45.0%
!!	GTTTGGTGTCCTAGCTATCT+TGG	+	chr1.1:825898-825917	None:intergenic	45.0%
	AATGGCGATGAACTCCTTGG+AGG	-	chr1.1:825747-825766	MS.gene27051:CDS	50.0%
	AGGAGGAGGATGTTCAGTTG+GGG	+	chr1.1:825370-825389	None:intergenic	50.0%
	CGGTCTTCAACTGAGAAGGT+TGG	+	chr1.1:825552-825571	None:intergenic	50.0%
	GACAACAACTCACGCTTGCA+TGG	-	chr1.1:825231-825250	MS.gene27051:CDS	50.0%
	GCAAGCGTGAGTTGTTGTCA+AGG	+	chr1.1:825230-825249	None:intergenic	50.0%
	GGCTAAGTGAGTTGCCTTGA+TGG	+	chr1.1:825803-825822	None:intergenic	50.0%
	GGTGGACCTCTCTAGTTGTT+GGG	+	chr1.1:825188-825207	None:intergenic	50.0%
	GTGGAACCCAACAACTAGAG+AGG	-	chr1.1:825179-825198	MS.gene27051:CDS	50.0%
	GTTGCGGTCTTCAACTGAGA+AGG	+	chr1.1:825556-825575	None:intergenic	50.0%
	TTCAGTTGGGGTCCAAGAGT+TGG	+	chr1.1:825358-825377	None:intergenic	50.0%
!	ATGCTCGGCCTTTGGAAGAA+TGG	-	chr1.1:825729-825748	MS.gene27051:CDS	50.0%
	AGGACACAGGCGTCTGATCT+TGG	+	chr1.1:825397-825416	None:intergenic	55.0%
	CGGTGGACCTCTCTAGTTGT+TGG	+	chr1.1:825189-825208	None:intergenic	55.0%
	GTTGACAAGGGGGCTATGCT+TGG	+	chr1.1:825876-825895	None:intergenic	55.0%
	TGTCTCGTATGCTCGGCCTT+TGG	-	chr1.1:825721-825740	MS.gene27051:CDS	55.0%
	TTGTCAAGGCAGAGACTCGG+AGG	+	chr1.1:825216-825235	None:intergenic	55.0%
	AGAGACTCGGAGGAACACGG+TGG	+	chr1.1:825206-825225	None:intergenic	60.0%
	GGCAGAGACTCGGAGGAACA+CGG	+	chr1.1:825209-825228	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	825177	825920	825177	ID=MS.gene27051
chr1.1	mRNA	825177	825920	825177	ID=MS.gene27051.t1;Parent=MS.gene27051
chr1.1	exon	825177	825920	825177	ID=MS.gene27051.t1.exon1;Parent=MS.gene27051.t1
chr1.1	CDS	825177	825920	825177	ID=cds.MS.gene27051.t1;Parent=MS.gene27051.t1

Gene Sequence

>MS.gene27051

ATGTGGAACCCAACAACTAGAGAGGTCCACCGTGTTCCTCCGAGTCTCTGCCTTGACAACAACTCACGCTTGCATGGTTTTGGTGCTGATGATCCCAACAACATCAGCTTCAAAGTTGTGAAACTCTATATAAGTTATGATGGAATTAGGAGATCAGCGGAAGTTTATAATCTAAGTACCAACTCTTGGACCCCAACTGAACATCCTCCTCCTTTTACCAAGATCAGACGCCTGTGTCCTTCTAGATATAACACTCTTGTTAACGGCGTTTATCATTGGATTACAAACAATTCACTCTACTATAGCAATAATGGTGTTGCAAATATTCTATGCTTTGACTTTCGCAACAATCAGTTTCACGAACTTAGAGGGCCAACCTTCTCAGTTGAAGACCGCAACTTTCTACGCGATGATGTTGCCGAGATTAAGGGTTCCCTTACTTATGTTACACAGAATCGCTTCGATTCTCCTGTCGTTTCGAACATTTGGGTAATGGATCAAAGTGGATGGCACAAAAAGTACAACATTGGCCCACCTGTATCAATGTCTCGTATGCTCGGCCTTTGGAAGAATGGCGATGAACTCCTTGGAGGAAAGTTTGGAGAACCGCTCAAATCATATGACCATCAAGGCAACTCACTTAGCCAATTTCAAATTGATGTTCATAAGACTAAATATTGGATTCAGGAGTATGTGCCAAGCATAGCCCCCTTGTCAACCAAGATAGCTAGGACACCAAACTAG

Protein sequence

>MS.gene27051.t1

MWNPTTREVHRVPPSLCLDNNSRLHGFGADDPNNISFKVVKLYISYDGIRRSAEVYNLSTNSWTPTEHPPPFTKIRRLCPSRYNTLVNGVYHWITNNSLYYSNNGVANILCFDFRNNQFHELRGPTFSVEDRNFLRDDVAEIKGSLTYVTQNRFDSPVVSNIWVMDQSGWHKKYNIGPPVSMSRMLGLWKNGDELLGGKFGEPLKSYDHQGNSLSQFQIDVHKTKYWIQEYVPSIAPLSTKIARTPN