AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033441

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033441.t1 MTR_8g102520 76.220 164 39 0 3 166 149 312 1.97e-87 259
MS.gene033441.t1 MTR_8g098830 64.943 174 53 2 3 169 54 226 2.00e-74 223
MS.gene033441.t1 MTR_7g079450 80.000 130 22 1 37 166 2 127 2.89e-71 211
MS.gene033441.t1 MTR_7g079400 63.415 164 60 0 2 165 159 322 1.27e-69 214
MS.gene033441.t1 MTR_7g079370 58.182 165 68 1 2 166 183 346 3.08e-55 178
MS.gene033441.t1 MTR_7g079420 59.064 171 60 5 2 166 968 1134 3.64e-55 187
MS.gene033441.t1 MTR_2g048580 53.529 170 61 6 2 166 176 332 1.83e-47 157
MS.gene033441.t1 MTR_4g117720 45.930 172 81 5 2 166 201 367 8.35e-41 141
MS.gene033441.t1 MTR_6g060650 48.193 166 78 4 3 166 204 363 1.23e-40 140
MS.gene033441.t1 MTR_4g109280 47.647 170 77 7 2 166 187 349 1.96e-38 134
MS.gene033441.t1 MTR_4g116320 48.701 154 74 3 16 166 97 248 2.14e-37 129
MS.gene033441.t1 MTR_1g055175 40.719 167 95 3 2 166 27 191 1.51e-36 125
MS.gene033441.t1 MTR_5g070088 48.052 154 74 4 17 166 224 375 3.77e-36 129
MS.gene033441.t1 MTR_4g118380 48.936 141 63 2 17 148 217 357 4.20e-36 128
MS.gene033441.t1 MTR_4g094365 48.077 156 73 5 16 166 202 354 2.66e-35 126
MS.gene033441.t1 MTR_4g116330 48.387 155 73 4 17 166 218 370 1.33e-34 125
MS.gene033441.t1 MTR_5g070080 47.742 155 73 5 16 166 225 375 7.19e-34 123
MS.gene033441.t1 MTR_5g097210 44.048 168 85 6 2 166 192 353 1.64e-33 122
MS.gene033441.t1 MTR_1g055195 42.105 152 87 1 15 166 318 468 1.89e-33 123
MS.gene033441.t1 MTR_5g070130 47.020 151 74 4 20 166 218 366 1.92e-33 122
MS.gene033441.t1 MTR_1g055165 41.250 160 90 3 9 166 151 308 1.93e-33 120
MS.gene033441.t1 MTR_4g023130 46.497 157 70 5 16 166 155 303 6.11e-33 119
MS.gene033441.t1 MTR_4g023080 45.161 155 75 4 16 166 196 344 6.28e-33 120
MS.gene033441.t1 MTR_4g118400 41.317 167 78 5 2 166 142 290 1.41e-32 118
MS.gene033441.t1 MTR_4g122850 44.172 163 84 6 8 166 195 354 3.63e-32 119
MS.gene033441.t1 MTR_3g103020 41.420 169 89 5 2 166 206 368 4.01e-31 115
MS.gene033441.t1 MTR_1g055135 39.881 168 98 2 2 166 198 365 6.74e-31 115
MS.gene033441.t1 MTR_4g109270 40.588 170 92 5 2 166 189 354 9.41e-31 114
MS.gene033441.t1 MTR_4g021785 41.860 172 84 6 1 166 184 345 1.02e-30 114
MS.gene033441.t1 MTR_8g040935 39.535 172 93 6 2 166 31 198 2.06e-30 110
MS.gene033441.t1 MTR_4g118360 48.276 116 59 1 52 166 1 116 4.32e-30 107
MS.gene033441.t1 MTR_8g433030 46.104 154 73 6 18 166 184 332 4.56e-30 112
MS.gene033441.t1 MTR_8g032390 46.104 154 73 6 18 166 217 365 6.36e-30 112
MS.gene033441.t1 MTR_3g034180 44.937 158 73 5 15 166 330 479 1.58e-29 113
MS.gene033441.t1 MTR_4g117750 47.468 158 75 3 15 166 194 349 2.46e-29 112
MS.gene033441.t1 MTR_4g117750 43.137 102 53 3 69 166 373 473 7.58e-14 68.9
MS.gene033441.t1 MTR_3g034130 42.958 142 67 3 15 149 216 350 3.76e-29 110
MS.gene033441.t1 MTR_4g023190 42.169 166 87 4 7 166 187 349 8.12e-29 109
MS.gene033441.t1 MTR_1g055250 40.764 157 85 5 15 170 110 259 9.78e-29 108
MS.gene033441.t1 MTR_4g118370 42.105 152 68 3 15 166 119 250 2.75e-28 105
MS.gene033441.t1 MTR_4g117850 40.000 160 87 4 14 166 213 370 2.42e-27 105
MS.gene033441.t1 MTR_4g023070 39.157 166 79 5 18 166 200 360 3.31e-27 105
MS.gene033441.t1 MTR_4g023200 41.401 157 83 4 16 166 197 350 1.75e-26 103
MS.gene033441.t1 MTR_6g463430 40.828 169 89 6 2 167 33 193 7.47e-26 98.2
MS.gene033441.t1 MTR_4g023150 41.290 155 82 4 18 166 199 350 8.32e-26 101
MS.gene033441.t1 MTR_3g034160 41.401 157 80 4 15 166 216 365 3.06e-25 100
MS.gene033441.t1 MTR_4g023090 39.053 169 80 7 16 166 211 374 3.17e-25 100
MS.gene033441.t1 MTR_4g023120 39.157 166 79 6 18 166 200 360 6.05e-25 99.4
MS.gene033441.t1 MTR_8g433040 46.970 132 62 4 38 166 228 354 1.15e-24 98.6
MS.gene033441.t1 MTR_8g032380 46.970 132 62 4 38 166 228 354 1.15e-24 98.6
MS.gene033441.t1 MTR_4g117770 43.312 157 83 4 15 166 202 357 1.30e-24 98.2
MS.gene033441.t1 MTR_4g117810 43.312 157 83 4 15 166 202 357 1.30e-24 98.2
MS.gene033441.t1 MTR_4g117780 40.476 168 92 5 4 166 193 357 1.12e-23 95.9
MS.gene033441.t1 MTR_4g023380 36.723 177 81 5 16 166 196 367 1.62e-23 95.5
MS.gene033441.t1 MTR_4g117530 34.731 167 70 5 2 166 114 243 8.64e-22 89.0
MS.gene033441.t1 MTR_4g117870 45.000 120 61 4 49 166 141 257 4.16e-21 87.4
MS.gene033441.t1 MTR_5g023000 50.602 83 38 2 86 166 8 89 3.66e-18 75.5
MS.gene033441.t1 MTR_4g116330 47.170 106 51 3 66 166 226 331 4.86e-18 80.1
MS.gene033441.t1 MTR_4g023440 32.934 167 66 4 2 166 200 322 2.47e-16 75.5
MS.gene033441.t1 MTR_7g117720 48.387 93 39 4 81 166 177 267 1.31e-14 70.1
MS.gene033441.t1 MTR_4g122860 38.710 124 58 6 53 166 195 310 1.69e-14 70.1
MS.gene033441.t1 MTR_4g114700 44.444 108 52 4 70 170 114 220 4.45e-14 68.2
MS.gene033441.t1 MTR_4g117430 44.737 76 38 2 2 77 80 151 2.67e-13 64.3
MS.gene033441.t1 MTR_6g021880 32.800 125 78 4 3 124 215 336 4.85e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATTGCCTTTGATTTAA+AGG 0.116438 7.2:-25995352 MS.gene033441:CDS
ATTTATGATTTGATGGTATT+TGG 0.157846 7.2:-25995270 MS.gene033441:CDS
CTGGCATGTACTTGCCTTAT+AGG 0.174261 7.2:-25995470 None:intergenic
CTCTGTATACACCGCCATTT+TGG 0.207122 7.2:+25994996 None:intergenic
CACGATTTGTAAAGCATATT+GGG 0.232410 7.2:+25995121 None:intergenic
CCACGATTTGTAAAGCATAT+TGG 0.239313 7.2:+25995120 None:intergenic
GGCAATAATAACATCGCTTT+TGG 0.269149 7.2:+25995365 None:intergenic
CCGACTCTGGGACCACCTTC+AGG 0.271870 7.2:+25995435 None:intergenic
TCTTAGAATTTATGATTTGA+TGG 0.287400 7.2:-25995277 MS.gene033441:CDS
TCTTGAATGGTTCTATTCAT+TGG 0.326345 7.2:-25995407 MS.gene033441:CDS
GGGCTTGCAATATTCCTATA+AGG 0.347352 7.2:+25995456 None:intergenic
TTACTTTATGAGATCCAAAA+TGG 0.350207 7.2:-25995010 MS.gene033441:CDS
GAATGGTTCTATTCATTGGC+TGG 0.382596 7.2:-25995403 MS.gene033441:CDS
AGAGTTCTAAAATTAAGATA+TGG 0.383416 7.2:-25995221 MS.gene033441:CDS
TTGGCGGTCTTATCAGTGTA+TGG 0.423905 7.2:-25995251 MS.gene033441:CDS
TGACCACTATTAAAATCATC+TGG 0.425662 7.2:+25995303 None:intergenic
CTGAAGGTGGTCCCAGAGTC+GGG 0.433638 7.2:-25995434 MS.gene033441:CDS
CGACTCTGGGACCACCTTCA+GGG 0.442516 7.2:+25995436 None:intergenic
ACAAATCGTGGTGATATAGT+TGG 0.444100 7.2:-25995108 MS.gene033441:CDS
AATACGCAGTGCATTCATCT+TGG 0.467778 7.2:-25995188 MS.gene033441:CDS
TGTCATTTCCTTTAAATCAA+AGG 0.469429 7.2:+25995344 None:intergenic
ATTCAAGAATGACCCGACTC+TGG 0.470304 7.2:+25995422 None:intergenic
GAGTTCTAAAATTAAGATAT+GGG 0.472837 7.2:-25995220 MS.gene033441:CDS
GCTAATCCACCATCAGCAAC+TGG 0.479576 7.2:+25995084 None:intergenic
TATGATTTGATGGTATTTGG+CGG 0.507303 7.2:-25995267 MS.gene033441:CDS
AGGAATATTGCAAGCCCTGA+AGG 0.512532 7.2:-25995450 MS.gene033441:CDS
CTGCAAGAGTATCATTCCTA+TGG 0.523562 7.2:-25995039 MS.gene033441:CDS
CCTGAAGGTGGTCCCAGAGT+CGG 0.527660 7.2:-25995435 MS.gene033441:CDS
CCAATATGCTTTACAAATCG+TGG 0.535731 7.2:-25995120 MS.gene033441:CDS
AGAGTCGGGTCATTCTTGAA+TGG 0.540114 7.2:-25995420 MS.gene033441:CDS
TTCAAGAATGACCCGACTCT+GGG 0.541376 7.2:+25995423 None:intergenic
GTTGGTCCAGTTGCTGATGG+TGG 0.544814 7.2:-25995090 MS.gene033441:CDS
ATAGTTGGTCCAGTTGCTGA+TGG 0.578433 7.2:-25995093 MS.gene033441:CDS
CATAAAGTAACGGTCACCAT+AGG 0.590340 7.2:+25995023 None:intergenic
ACGATTTGTAAAGCATATTG+GGG 0.609876 7.2:+25995122 None:intergenic
TCTCTGCTTTCACTCCCCGA+TGG 0.634589 7.2:-25994971 MS.gene033441:intron
TTAGCGAAGCTTAATGACAA+AGG 0.665656 7.2:-25995066 MS.gene033441:CDS
AATATTGCAAGCCCTGAAGG+TGG 0.669896 7.2:-25995447 MS.gene033441:CDS
CTTTATGAGATCCAAAATGG+CGG 0.689205 7.2:-25995007 MS.gene033441:CDS
AAGTTTGCCCAATACCATCG+GGG 0.746553 7.2:+25994957 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAGATAGATATCATTAAAA+TGG + chr7.2:25995430-25995449 None:intergenic 20.0%
!! TCTTAGAATTTATGATTTGA+TGG - chr7.2:25995029-25995048 MS.gene033441:CDS 20.0%
!!! AGAGTTCTAAAATTAAGATA+TGG - chr7.2:25995085-25995104 MS.gene033441:CDS 20.0%
!!! ATTTATGATTTGATGGTATT+TGG - chr7.2:25995036-25995055 MS.gene033441:CDS 20.0%
!!! GAGTTCTAAAATTAAGATAT+GGG - chr7.2:25995086-25995105 MS.gene033441:CDS 20.0%
!!! TATTATTGCCTTTGATTTAA+AGG - chr7.2:25994954-25994973 MS.gene033441:intron 20.0%
! ACAATCAAATCTTTCTTGTT+TGG + chr7.2:25995391-25995410 None:intergenic 25.0%
! TATCTTGTATTATGAACTCT+TGG - chr7.2:25995443-25995462 MS.gene033441:CDS 25.0%
! TTACTTTATGAGATCCAAAA+TGG - chr7.2:25995296-25995315 MS.gene033441:CDS 25.0%
!! TGTCATTTCCTTTAAATCAA+AGG + chr7.2:25994965-25994984 None:intergenic 25.0%
!! TTTTGGATCTCATAAAGTAA+CGG + chr7.2:25995296-25995315 None:intergenic 25.0%
ACGATTTGTAAAGCATATTG+GGG + chr7.2:25995187-25995206 None:intergenic 30.0%
CACGATTTGTAAAGCATATT+GGG + chr7.2:25995188-25995207 None:intergenic 30.0%
TGACCACTATTAAAATCATC+TGG + chr7.2:25995006-25995025 None:intergenic 30.0%
! TATGATTTGATGGTATTTGG+CGG - chr7.2:25995039-25995058 MS.gene033441:CDS 30.0%
! TCTTGAATGGTTCTATTCAT+TGG - chr7.2:25994899-25994918 MS.gene033441:intron 30.0%
!! CTTCCAGATGATTTTAATAG+TGG - chr7.2:25995000-25995019 MS.gene033441:CDS 30.0%
AAGCATATTGGGGAAAAATC+CGG + chr7.2:25995177-25995196 None:intergenic 35.0%
ACAAATCGTGGTGATATAGT+TGG - chr7.2:25995198-25995217 MS.gene033441:CDS 35.0%
CCAATATGCTTTACAAATCG+TGG - chr7.2:25995186-25995205 MS.gene033441:CDS 35.0%
CCACGATTTGTAAAGCATAT+TGG + chr7.2:25995189-25995208 None:intergenic 35.0%
CTTTATGAGATCCAAAATGG+CGG - chr7.2:25995299-25995318 MS.gene033441:CDS 35.0%
TTAAGTTTGCCCAATACCAT+CGG + chr7.2:25995354-25995373 None:intergenic 35.0%
TTAGCGAAGCTTAATGACAA+AGG - chr7.2:25995240-25995259 MS.gene033441:CDS 35.0%
! GGCAATAATAACATCGCTTT+TGG + chr7.2:25994944-25994963 None:intergenic 35.0%
AATACGCAGTGCATTCATCT+TGG - chr7.2:25995118-25995137 MS.gene033441:CDS 40.0%
ACTCTTGGTATCATGAAGCA+TGG - chr7.2:25995458-25995477 MS.gene033441:CDS 40.0%
CATAAAGTAACGGTCACCAT+AGG + chr7.2:25995286-25995305 None:intergenic 40.0%
GGGCTTGCAATATTCCTATA+AGG + chr7.2:25994853-25994872 None:intergenic 40.0%
TAAGTTTGCCCAATACCATC+GGG + chr7.2:25995353-25995372 None:intergenic 40.0%
! GAATGGTTCTATTCATTGGC+TGG - chr7.2:25994903-25994922 MS.gene033441:intron 40.0%
! TTTTTCTTTTCATCCCGCTC+CGG - chr7.2:25995155-25995174 MS.gene033441:CDS 40.0%
!! CTGCAAGAGTATCATTCCTA+TGG - chr7.2:25995267-25995286 MS.gene033441:CDS 40.0%
AAGTTTGCCCAATACCATCG+GGG + chr7.2:25995352-25995371 None:intergenic 45.0%
AATATTGCAAGCCCTGAAGG+TGG - chr7.2:25994859-25994878 MS.gene033441:intron 45.0%
AGAGTCGGGTCATTCTTGAA+TGG - chr7.2:25994886-25994905 MS.gene033441:intron 45.0%
AGGAATATTGCAAGCCCTGA+AGG - chr7.2:25994856-25994875 MS.gene033441:intron 45.0%
ATAGTTGGTCCAGTTGCTGA+TGG - chr7.2:25995213-25995232 MS.gene033441:CDS 45.0%
ATTCAAGAATGACCCGACTC+TGG + chr7.2:25994887-25994906 None:intergenic 45.0%
TATTGGGGAAAAATCCGGAG+CGG + chr7.2:25995172-25995191 None:intergenic 45.0%
TTCAAGAATGACCCGACTCT+GGG + chr7.2:25994886-25994905 None:intergenic 45.0%
TTTCACTCCCCGATGGTATT+GGG - chr7.2:25995342-25995361 MS.gene033441:CDS 45.0%
! CTCTGTATACACCGCCATTT+TGG + chr7.2:25995313-25995332 None:intergenic 45.0%
! TTGGCGGTCTTATCAGTGTA+TGG - chr7.2:25995055-25995074 MS.gene033441:CDS 45.0%
ATTGGGGAAAAATCCGGAGC+GGG + chr7.2:25995171-25995190 None:intergenic 50.0%
CTTTCACTCCCCGATGGTAT+TGG - chr7.2:25995341-25995360 MS.gene033441:CDS 50.0%
GCTAATCCACCATCAGCAAC+TGG + chr7.2:25995225-25995244 None:intergenic 50.0%
GTTGGTCCAGTTGCTGATGG+TGG - chr7.2:25995216-25995235 MS.gene033441:CDS 55.0%
TCTCTGCTTTCACTCCCCGA+TGG - chr7.2:25995335-25995354 MS.gene033441:CDS 55.0%
CCTGAAGGTGGTCCCAGAGT+CGG - chr7.2:25994871-25994890 MS.gene033441:intron 60.0%
CTGAAGGTGGTCCCAGAGTC+GGG - chr7.2:25994872-25994891 MS.gene033441:intron 60.0%
!! CGACTCTGGGACCACCTTCA+GGG + chr7.2:25994873-25994892 None:intergenic 60.0%
!! CCGACTCTGGGACCACCTTC+AGG + chr7.2:25994874-25994893 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 25994841 25995487 25994841 ID=MS.gene033441
chr7.2 mRNA 25994841 25995487 25994841 ID=MS.gene033441.t1;Parent=MS.gene033441
chr7.2 exon 25994972 25995487 25994972 ID=MS.gene033441.t1.exon1;Parent=MS.gene033441.t1
chr7.2 CDS 25994972 25995487 25994972 ID=cds.MS.gene033441.t1;Parent=MS.gene033441.t1
chr7.2 exon 25994841 25994852 25994841 ID=MS.gene033441.t1.exon2;Parent=MS.gene033441.t1
chr7.2 CDS 25994841 25994852 25994841 ID=cds.MS.gene033441.t1;Parent=MS.gene033441.t1
Gene Sequence

>MS.gene033441

ATGTACTTGCCTTATAGGAATATTGCAAGCCCTGAAGGTGGTCCCAGAGTCGGGTCATTCTTGAATGGTTCTATTCATTGGCTGGTTTATAATTATGAGACCAAAAGCGATGTTATTATTGCCTTTGATTTAAAGGAAATGACACTGTTAGAGATAGCTCTTCCAGATGATTTTAATAGTGGTCACTCTCTTAGAATTTATGATTTGATGGTATTTGGCGGTCTTATCAGTGTATGGATTGTGAAGAGTTCTAAAATTAAGATATGGGTAATGCAAGAATACGCAGTGCATTCATCTTGGACTAAGACTCTTGATTTTTCTTTTCATCCCGCTCCGGATTTTTCCCCAATATGCTTTACAAATCGTGGTGATATAGTTGGTCCAGTTGCTGATGGTGGATTAGCGAAGCTTAATGACAAAGGACAACTGCAAGAGTATCATTCCTATGGTGACCGTTACTTTATGAGATCCAAAATGGCGGTGTATACAGAGTCTCTCTGCTTTCACTCCCCGATGGTATTGGGCAAACTTAAGATAATTAGTGAAGCCAAACAAGAAAGATTTGATTGTGAAATATTTGTATTTACCATTTTAATGATATCTATCTTGTATTATGAACTCTTGGTATCATGAAGCATGGAAAGTGA
Protein sequence

>MS.gene033441.t1

MYLPYRNIASPEGGPRVGSFLNGSIHWLVYNYETKSDVIIAFDLKEMTLLEIALPDDFNSGHSLRIYDLMVFGGLISVWIVKSSKIKIWVMQEYAVHSSWTKTLDFSFHPAPDFSPICFTNRGDIVGPVADGGLAKLNDKGQLQEYHSYGDRYFMRSKMAVYTESLCFHSPMHGK