AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57526

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57526.t1 MTR_8g433030 73.077 338 57 5 2 330 24 336 1.10e-165 465
MS.gene57526.t1 MTR_8g032390 71.839 348 77 6 2 330 24 369 4.77e-165 465
MS.gene57526.t1 MTR_8g433040 66.048 377 74 5 1 336 1 364 2.10e-159 450
MS.gene57526.t1 MTR_8g032380 66.048 377 74 5 1 336 1 364 2.10e-159 450
MS.gene57526.t1 MTR_3g103020 49.319 367 138 11 14 336 16 378 1.56e-97 293
MS.gene57526.t1 MTR_4g023080 49.311 363 131 12 13 335 4 353 4.21e-96 289
MS.gene57526.t1 MTR_4g094365 48.649 370 136 9 13 336 3 364 8.34e-95 286
MS.gene57526.t1 MTR_4g021785 47.826 368 136 13 11 336 2 355 7.84e-91 275
MS.gene57526.t1 MTR_4g109270 48.518 371 125 13 18 336 8 364 1.37e-90 275
MS.gene57526.t1 MTR_4g023130 50.000 336 128 11 13 335 4 312 1.97e-90 273
MS.gene57526.t1 MTR_4g122850 51.117 358 120 14 29 336 12 364 4.88e-90 275
MS.gene57526.t1 MTR_4g023070 47.170 371 139 11 18 335 3 369 3.00e-89 272
MS.gene57526.t1 MTR_3g034180 48.698 384 135 16 8 336 113 489 3.55e-89 276
MS.gene57526.t1 MTR_4g023090 44.330 388 152 13 7 336 5 386 9.44e-87 266
MS.gene57526.t1 MTR_4g117850 47.191 356 141 10 17 329 22 373 1.31e-86 265
MS.gene57526.t1 MTR_4g109280 49.171 362 133 16 17 336 7 359 8.25e-85 260
MS.gene57526.t1 MTR_3g034130 49.046 367 128 14 11 323 2 363 9.15e-85 261
MS.gene57526.t1 MTR_4g023120 45.430 372 146 11 18 336 3 370 1.19e-83 258
MS.gene57526.t1 MTR_4g116330 44.474 380 159 12 3 336 7 380 5.80e-82 254
MS.gene57526.t1 MTR_3g034160 48.011 377 136 14 13 335 4 374 4.34e-81 251
MS.gene57526.t1 MTR_6g060650 46.859 382 132 15 13 335 3 372 6.95e-80 248
MS.gene57526.t1 MTR_5g097210 45.455 363 154 10 11 333 2 360 1.19e-79 248
MS.gene57526.t1 MTR_4g117720 44.565 368 124 12 7 330 40 371 3.65e-79 246
MS.gene57526.t1 MTR_5g070080 42.494 393 154 14 9 336 1 386 6.84e-75 236
MS.gene57526.t1 MTR_4g023380 43.622 392 134 13 11 336 9 379 1.05e-73 233
MS.gene57526.t1 MTR_4g023190 44.565 368 151 11 9 334 1 357 3.65e-73 230
MS.gene57526.t1 MTR_5g070130 43.048 374 157 12 9 330 1 370 3.93e-73 231
MS.gene57526.t1 MTR_4g117780 44.173 369 144 14 18 336 11 367 4.37e-73 230
MS.gene57526.t1 MTR_4g023150 44.595 370 149 12 9 334 1 358 1.52e-71 226
MS.gene57526.t1 MTR_4g117770 44.297 377 145 14 13 336 3 367 1.06e-70 224
MS.gene57526.t1 MTR_4g117810 44.297 377 145 14 13 336 3 367 1.06e-70 224
MS.gene57526.t1 MTR_4g023200 43.902 369 153 11 9 334 1 358 1.18e-69 221
MS.gene57526.t1 MTR_1g055135 42.593 378 159 12 10 336 5 375 1.30e-69 222
MS.gene57526.t1 MTR_4g118380 45.938 357 121 13 17 309 7 355 1.68e-67 216
MS.gene57526.t1 MTR_4g116320 55.349 215 87 4 126 333 43 255 7.32e-66 208
MS.gene57526.t1 MTR_4g023440 42.440 377 131 14 1 336 1 332 1.53e-65 210
MS.gene57526.t1 MTR_4g121580 39.782 367 139 11 11 326 4 339 6.34e-65 210
MS.gene57526.t1 MTR_1g055165 41.813 342 161 10 9 336 1 318 9.88e-61 197
MS.gene57526.t1 MTR_8g040935 53.922 204 84 4 143 336 5 208 4.29e-60 192
MS.gene57526.t1 MTR_4g117750 43.052 367 153 14 16 335 1 358 3.63e-59 197
MS.gene57526.t1 MTR_4g117750 54.386 114 49 2 224 334 368 481 1.68e-29 118
MS.gene57526.t1 MTR_4g116330 38.158 380 144 12 3 336 7 341 6.05e-57 188
MS.gene57526.t1 MTR_1g055195 49.160 238 84 9 118 336 259 478 1.13e-55 189
MS.gene57526.t1 MTR_6g463430 53.886 193 77 6 151 336 15 202 1.55e-55 180
MS.gene57526.t1 MTR_8g013010 45.221 272 86 11 16 238 52 309 2.52e-54 181
MS.gene57526.t1 MTR_1g055175 50.256 195 85 5 151 335 8 200 2.68e-53 174
MS.gene57526.t1 MTR_1g055250 44.177 249 121 8 100 336 28 270 1.49e-51 174
MS.gene57526.t1 MTR_4g117530 37.963 324 105 8 17 336 22 253 1.49e-51 171
MS.gene57526.t1 MTR_4g118370 46.429 224 90 6 119 333 55 257 2.47e-51 171
MS.gene57526.t1 MTR_4g117870 44.749 219 84 4 120 336 84 267 2.91e-48 163
MS.gene57526.t1 MTR_7g079400 45.923 233 105 9 120 336 110 337 1.55e-47 163
MS.gene57526.t1 MTR_5g070088 45.175 228 111 7 120 336 162 386 3.67e-46 161
MS.gene57526.t1 MTR_5g070088 53.012 83 28 3 12 86 1 80 1.57e-13 71.2
MS.gene57526.t1 MTR_8g102520 44.105 229 111 8 120 336 99 322 5.57e-45 156
MS.gene57526.t1 MTR_4g122860 34.006 347 136 12 4 336 53 320 4.53e-42 149
MS.gene57526.t1 MTR_4g118400 50.000 190 70 8 149 330 122 294 6.34e-41 145
MS.gene57526.t1 MTR_7g079370 44.298 228 102 10 120 330 131 350 7.14e-41 146
MS.gene57526.t1 MTR_4g116340 39.749 239 106 10 1 204 12 247 2.83e-35 129
MS.gene57526.t1 MTR_2g048580 32.787 366 178 17 13 336 3 342 3.00e-34 129
MS.gene57526.t1 MTR_4g118360 55.469 128 48 5 214 335 1 125 3.40e-33 119
MS.gene57526.t1 MTR_8g098830 42.857 189 90 9 152 330 35 215 3.54e-33 123
MS.gene57526.t1 MTR_4g114700 37.553 237 85 8 104 327 32 218 3.96e-32 120
MS.gene57526.t1 MTR_4g117430 51.220 123 45 4 120 238 41 152 4.93e-29 109
MS.gene57526.t1 MTR_5g023000 59.596 99 37 2 241 336 1 99 1.70e-28 106
MS.gene57526.t1 MTR_7g117720 34.328 335 143 14 9 330 1 271 2.67e-28 111
MS.gene57526.t1 MTR_7g079420 35.556 225 106 9 120 336 951 1144 9.15e-26 108
MS.gene57526.t1 MTR_1g097490 29.143 350 201 14 18 328 49 390 9.33e-26 106
MS.gene57526.t1 MTR_7g079450 42.466 146 68 5 198 336 1 137 4.51e-25 98.6
MS.gene57526.t1 MTR_3g036430 29.252 294 170 12 18 289 20 297 9.17e-24 100
MS.gene57526.t1 MTR_2g055970 28.497 386 198 19 4 331 198 563 4.89e-23 100
MS.gene57526.t1 MTR_5g057690 30.986 284 156 10 18 269 46 321 6.59e-23 98.6
MS.gene57526.t1 MTR_3g007360 28.535 389 201 16 6 329 27 403 1.76e-22 97.4
MS.gene57526.t1 MTR_1g094210 25.627 359 202 13 18 328 57 398 1.80e-22 97.4
MS.gene57526.t1 MTR_4g108820 30.797 276 145 14 17 271 41 291 3.77e-22 95.5
MS.gene57526.t1 MTR_2g021150 25.479 365 218 13 17 332 13 372 5.86e-22 95.5
MS.gene57526.t1 MTR_8g101580 27.966 354 208 14 18 328 45 394 1.55e-21 94.7
MS.gene57526.t1 MTR_1g094210 26.113 337 185 12 18 307 57 376 1.58e-21 94.7
MS.gene57526.t1 MTR_6g034720 52.000 100 44 2 120 216 72 170 1.77e-21 91.3
MS.gene57526.t1 MTR_5g057020 30.389 283 154 10 18 269 46 316 4.16e-21 92.8
MS.gene57526.t1 MTR_0044s0300 29.721 323 162 13 2 273 16 324 5.76e-21 93.2
MS.gene57526.t1 MTR_5g057790 29.966 297 152 10 18 269 46 331 8.68e-21 92.4
MS.gene57526.t1 MTR_3g009310 31.293 294 150 10 18 268 42 326 1.13e-20 92.4
MS.gene57526.t1 MTR_1g097330 30.104 289 163 10 18 271 16 300 1.76e-20 92.0
MS.gene57526.t1 MTR_3g007410 27.835 388 203 16 7 329 28 403 2.28e-20 91.7
MS.gene57526.t1 MTR_3g083640 27.132 387 200 19 7 328 33 402 3.13e-20 91.3
MS.gene57526.t1 MTR_1g097560 26.966 356 203 16 18 331 36 376 4.72e-20 90.1
MS.gene57526.t1 MTR_2g046920 27.090 299 163 11 17 271 23 310 7.16e-20 89.7
MS.gene57526.t1 MTR_5g057710 29.236 301 149 11 18 269 46 331 1.33e-19 89.4
MS.gene57526.t1 MTR_4g023430 46.667 105 48 3 239 336 119 222 4.35e-19 85.1
MS.gene57526.t1 MTR_4g023430 57.143 84 26 3 9 84 32 113 2.44e-16 77.4
MS.gene57526.t1 MTR_2g029990 26.301 365 200 14 19 328 52 402 9.59e-19 86.7
MS.gene57526.t1 MTR_3g007330 29.730 296 157 11 18 273 48 332 9.97e-19 86.7
MS.gene57526.t1 MTR_3g014570 30.034 293 157 9 18 268 29 315 1.17e-18 86.3
MS.gene57526.t1 MTR_1g058190 29.286 280 165 11 16 269 33 305 1.53e-18 85.9
MS.gene57526.t1 MTR_3g014540 30.537 298 159 10 18 273 29 320 2.70e-18 85.1
MS.gene57526.t1 MTR_4g061340 30.519 308 139 14 18 268 26 315 5.01e-18 84.3
MS.gene57526.t1 MTR_8g467140 28.146 302 160 12 14 269 28 318 9.65e-18 83.2
MS.gene57526.t1 MTR_7g013590 30.903 288 157 10 22 269 40 325 2.25e-17 82.8
MS.gene57526.t1 MTR_6g021880 28.612 353 197 13 29 328 31 381 3.51e-17 82.0
MS.gene57526.t1 MTR_4g055060 29.252 294 155 11 18 264 30 317 4.48e-17 81.6
MS.gene57526.t1 MTR_3g007430 29.655 290 155 10 21 268 46 328 4.74e-17 81.6
MS.gene57526.t1 MTR_3g036335 26.810 373 192 17 18 328 41 394 5.10e-17 81.6
MS.gene57526.t1 MTR_7g070440 27.213 305 170 13 12 266 1 303 6.54e-17 81.3
MS.gene57526.t1 MTR_4g122840 40.741 135 43 4 205 336 164 264 1.03e-16 79.3
MS.gene57526.t1 MTR_1g007410 24.937 397 207 14 9 325 13 398 1.08e-16 80.9
MS.gene57526.t1 MTR_3g023930 25.915 355 198 14 18 328 34 367 1.60e-16 80.1
MS.gene57526.t1 MTR_1g068835 25.517 290 175 9 18 268 4 291 4.02e-16 79.0
MS.gene57526.t1 MTR_7g407070 27.692 325 171 12 18 281 32 353 4.23e-16 79.0
MS.gene57526.t1 MTR_2g021050 25.298 336 210 11 18 315 5 337 4.96e-16 78.6
MS.gene57526.t1 MTR_3g009240 28.772 285 157 10 18 264 36 312 5.06e-16 78.6
MS.gene57526.t1 MTR_7g007300 23.545 378 221 11 1 328 86 445 5.10e-16 79.0
MS.gene57526.t1 MTR_2g006360 26.259 278 178 7 1 268 29 289 6.54e-16 78.2
MS.gene57526.t1 MTR_4g030830 24.557 395 204 17 18 328 9 393 7.27e-16 78.2
MS.gene57526.t1 MTR_0760s0010 27.714 350 197 13 23 328 52 389 8.01e-16 78.2
MS.gene57526.t1 MTR_2g021155 27.673 318 158 15 18 313 671 938 1.08e-15 78.6
MS.gene57526.t1 MTR_4g109290 40.741 135 55 5 17 135 8 133 1.15e-15 74.7
MS.gene57526.t1 MTR_3g013770 28.247 308 157 9 18 273 33 328 1.59e-15 77.4
MS.gene57526.t1 MTR_3g010390 26.211 351 193 14 18 306 33 379 1.65e-15 77.4
MS.gene57526.t1 MTR_3g006670 29.866 298 152 13 18 268 49 336 2.95e-15 76.6
MS.gene57526.t1 MTR_3g030350 25.620 363 211 13 18 328 35 390 4.36e-15 75.9
MS.gene57526.t1 MTR_7g028660 26.708 322 165 11 16 275 9 321 9.29e-15 74.7
MS.gene57526.t1 MTR_3g010870 25.485 361 206 16 18 328 37 384 1.79e-14 73.9
MS.gene57526.t1 MTR_3g036290 27.931 290 152 10 18 268 22 293 1.86e-14 73.9
MS.gene57526.t1 MTR_5g043605 25.602 332 204 13 17 327 24 333 2.96e-14 73.2
MS.gene57526.t1 MTR_3g464500 25.360 347 190 12 8 289 17 359 3.26e-14 73.6
MS.gene57526.t1 MTR_3g024110 28.041 296 159 13 18 273 101 382 4.05e-14 73.2
MS.gene57526.t1 MTR_3g010970 25.208 361 195 15 22 328 52 391 5.22e-14 72.8
MS.gene57526.t1 MTR_2g031680 23.529 357 224 10 18 330 6 357 5.92e-14 72.4
MS.gene57526.t1 MTR_3g030240 25.886 367 187 15 18 328 40 377 8.56e-14 72.0
MS.gene57526.t1 MTR_8g464770 25.330 379 214 15 13 330 7 377 1.11e-13 71.6
MS.gene57526.t1 MTR_2g025960 28.641 206 133 6 120 317 68 267 1.69e-13 70.5
MS.gene57526.t1 MTR_0667s0010 23.810 357 213 10 18 318 6 359 2.59e-13 70.9
MS.gene57526.t1 MTR_7g022670 27.239 268 155 8 18 264 23 271 2.75e-13 70.5
MS.gene57526.t1 MTR_6g007135 24.685 397 222 15 1 328 1 389 4.06e-13 70.1
MS.gene57526.t1 MTR_5g046260 27.037 270 150 12 18 264 4 249 4.52e-13 69.3
MS.gene57526.t1 MTR_1g068875 28.025 157 104 6 118 268 137 290 4.56e-13 69.7
MS.gene57526.t1 MTR_0831s0010 24.010 404 221 15 3 334 33 422 5.31e-13 69.7
MS.gene57526.t1 MTR_2g024110 24.739 287 175 9 18 264 4 289 8.16e-13 68.9
MS.gene57526.t1 MTR_3g024130 28.095 210 124 6 121 314 105 303 1.35e-12 67.8
MS.gene57526.t1 MTR_6g027910 28.279 244 150 11 96 328 145 374 1.52e-12 68.2
MS.gene57526.t1 MTR_1g068860 24.569 232 155 9 120 336 141 367 1.55e-12 68.2
MS.gene57526.t1 MTR_5g097350 26.697 221 145 9 120 332 175 386 1.96e-12 67.8
MS.gene57526.t1 MTR_7g056200 25.733 307 168 9 7 268 16 307 2.29e-12 67.8
MS.gene57526.t1 MTR_6g090180 26.496 234 146 8 118 329 205 434 2.35e-12 67.8
MS.gene57526.t1 MTR_7g055830 26.257 358 203 15 18 328 13 356 2.71e-12 67.4
MS.gene57526.t1 MTR_4g129190 27.103 321 158 15 18 272 6 316 3.15e-12 67.4
MS.gene57526.t1 MTR_6g092560 25.126 398 201 19 18 330 12 397 3.29e-12 67.4
MS.gene57526.t1 MTR_2g078870 29.560 159 98 6 116 272 95 241 5.17e-12 66.2
MS.gene57526.t1 MTR_2g026010 27.950 161 103 5 120 268 138 297 6.23e-12 66.6
MS.gene57526.t1 MTR_8g013005 60.714 56 20 1 282 335 3 58 6.88e-12 60.5
MS.gene57526.t1 MTR_8g062660 30.065 153 94 4 118 269 176 316 7.39e-12 66.6
MS.gene57526.t1 MTR_4g023100 60.000 55 22 0 282 336 65 119 1.02e-11 61.6
MS.gene57526.t1 MTR_7g009265 25.410 244 143 9 121 329 85 324 1.35e-11 65.1
MS.gene57526.t1 MTR_3g007270 29.193 161 105 4 120 277 200 354 1.53e-11 65.5
MS.gene57526.t1 MTR_6g017305 27.801 241 138 11 121 328 44 281 1.83e-11 64.3
MS.gene57526.t1 MTR_1g055145 45.161 93 44 4 167 255 113 202 2.98e-11 63.5
MS.gene57526.t1 MTR_5g056990 31.579 171 94 6 108 270 22 177 4.50e-11 62.4
MS.gene57526.t1 MTR_1g097370 25.556 180 119 5 122 289 135 311 8.05e-11 63.2
MS.gene57526.t1 MTR_0129s0050 25.410 244 145 6 120 329 164 404 8.44e-11 63.2
MS.gene57526.t1 MTR_8g099460 24.667 150 111 2 120 268 153 301 8.47e-11 63.2
MS.gene57526.t1 MTR_3g006500 26.852 216 134 8 122 328 169 369 9.70e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57526.t1 AT3G06240 29.545 220 129 9 121 328 214 419 3.17e-13 70.5
MS.gene57526.t1 AT4G12560 23.585 318 178 11 18 278 4 313 2.13e-11 65.1
MS.gene57526.t1 AT4G12560 23.585 318 178 11 18 278 4 313 2.13e-11 65.1
MS.gene57526.t1 AT4G12560 23.585 318 178 11 18 278 4 313 2.13e-11 65.1

Find 77 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTTGTCTTTGATTTAA+TGG 0.084690 8.3:-61979428 MS.gene57526:CDS
AAAGAAGGATTATTCTTTAA+TGG 0.186590 8.3:-61979499 MS.gene57526:CDS
AACCATGATCCTAAGTATTA+TGG 0.203715 8.3:-61979367 MS.gene57526:CDS
TATTATGGTTTGTGGGTATT+TGG 0.206725 8.3:-61979352 MS.gene57526:CDS
ATAATCCGGCACTTGAAATA+TGG 0.211797 8.3:-61979297 MS.gene57526:CDS
ATTAAAGAATAATCCTTCTT+TGG 0.223496 8.3:+61979501 None:intergenic
CTTCACATTATCATGATATA+CGG 0.241723 8.3:-61979459 MS.gene57526:CDS
TTAAAGAATAATCCTTCTTT+GGG 0.279731 8.3:+61979502 None:intergenic
AAGAAGGATTATTCTTTAAT+GGG 0.280888 8.3:-61979498 MS.gene57526:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.288501 8.3:-61979333 MS.gene57526:CDS
CCAAGTGATCTATATGGTTT+TGG 0.298158 8.3:-61979679 MS.gene57526:CDS
TCAAATCCTGCTGAGGTTGC+CGG 0.380355 8.3:-61980082 MS.gene57526:CDS
TCTTTAATGGGGCTATTCAT+TGG 0.381874 8.3:-61979486 MS.gene57526:CDS
ATGGCCACTTCAAATGCCTT+TGG 0.385262 8.3:+61979088 None:intergenic
TCCCCACTTCACTTATTTGA+AGG 0.387526 8.3:-61979527 MS.gene57526:CDS
CAAGGTATGTCATTGTCAAT+AGG 0.400804 8.3:+61979223 None:intergenic
TACCGTCCAAGTGATCTATA+TGG 0.403657 8.3:-61979685 MS.gene57526:CDS
CCTTTATCTGCAGAATTACA+TGG 0.405305 8.3:-61979709 MS.gene57526:intron
GGATCATGGTTGAAATCATC+TGG 0.411154 8.3:+61979379 None:intergenic
ATTTGAATGATTAATTTAGA+AGG 0.416433 8.3:+61980099 None:intergenic
ATCCTAAGTATTATGGTTTG+TGG 0.419854 8.3:-61979360 MS.gene57526:CDS
TAATGGGGCTATTCATTGGT+TGG 0.427301 8.3:-61979482 MS.gene57526:CDS
CGAATTTCAGCAAGAGGTCG+AGG 0.428388 8.3:+61979952 None:intergenic
CAACCATGATATTGTTTCAC+TGG 0.436168 8.3:-61979905 MS.gene57526:CDS
TAATCCGGCACTTGAAATAT+GGG 0.448185 8.3:-61979296 MS.gene57526:CDS
AGTGGTGATATTATTGGAAC+CGG 0.459631 8.3:-61979175 MS.gene57526:CDS
AATACAAACTGAAATCATCT+TGG 0.466347 8.3:-61979264 MS.gene57526:CDS
ATCACCCATATTTCAAGTGC+CGG 0.473975 8.3:+61979292 None:intergenic
TGAAACAATATCATGGTTGA+TGG 0.474061 8.3:+61979909 None:intergenic
TCAAATGCGTTTGTAAGTCA+TGG 0.482109 8.3:-61980041 MS.gene57526:CDS
TCCTAAGTATTATGGTTTGT+GGG 0.484785 8.3:-61979359 MS.gene57526:CDS
CCAAAACCATATAGATCACT+TGG 0.486613 8.3:+61979679 None:intergenic
CCGATCAAGAGATGATTACT+TGG 0.491735 8.3:-61979650 MS.gene57526:CDS
TATTTGAAGGTCCCCAAAGA+AGG 0.495324 8.3:-61979514 MS.gene57526:CDS
ACTTCACCGGCAACCTCAGC+AGG 0.499391 8.3:+61980076 None:intergenic
AACCATAATACTTAGGATCA+TGG 0.502897 8.3:+61979365 None:intergenic
CTATTGACAATGACATACCT+TGG 0.516935 8.3:-61979222 MS.gene57526:CDS
AAATTAAAGGTTCATGCAGA+GGG 0.521334 8.3:-61979844 MS.gene57526:intron
TTGTCTTTGATTTAATGGAA+AGG 0.526787 8.3:-61979423 MS.gene57526:CDS
ATCAAGAGATGATTACTTGG+TGG 0.531322 8.3:-61979647 MS.gene57526:CDS
TTTCATTGAGAGATAATACA+TGG 0.531728 8.3:-61979564 MS.gene57526:CDS
CACCAATCCATGACGACCAC+CGG 0.535909 8.3:+61979156 None:intergenic
GACCTTCAAATAAGTGAAGT+GGG 0.541109 8.3:+61979525 None:intergenic
TTCACATTATCATGATATAC+GGG 0.547983 8.3:-61979458 MS.gene57526:CDS
ATTGGAACCGGTGGTCGTCA+TGG 0.554919 8.3:-61979163 MS.gene57526:CDS
AGAAGGATTATTCTTTAATG+GGG 0.561686 8.3:-61979497 MS.gene57526:CDS
AACCGGTGGTCGTCATGGAT+TGG 0.561896 8.3:-61979158 MS.gene57526:CDS
CCTCGCGAAGAAGACATGTA+TGG 0.567307 8.3:+61979881 None:intergenic
GAAATTAAAGGTTCATGCAG+AGG 0.568478 8.3:-61979845 MS.gene57526:CDS
ACCCACAAACCATAATACTT+AGG 0.570029 8.3:+61979358 None:intergenic
TGATATGGACACAATTCTTG+CGG 0.574680 8.3:+61979978 None:intergenic
AAACATAAAAGAGACTTCAC+CGG 0.574747 8.3:+61980063 None:intergenic
TGGTTGAAATCATCTGGAAG+AGG 0.588065 8.3:+61979385 None:intergenic
GGTCCAGTGAAACAATATCA+TGG 0.591390 8.3:+61979902 None:intergenic
CCAAGTAATCATCTCTTGAT+CGG 0.593246 8.3:+61979650 None:intergenic
GGACCTTCAAATAAGTGAAG+TGG 0.596698 8.3:+61979524 None:intergenic
CATCTTGTTAAACTTGCTCA+CGG 0.601761 8.3:+61979032 None:intergenic
ATAGAGCGAATTTCAGCAAG+AGG 0.601938 8.3:+61979946 None:intergenic
TAATCATTCAAATCCTGCTG+AGG 0.603328 8.3:-61980089 MS.gene57526:CDS
AAGCAGAGACTCTTTATACA+TGG 0.605588 8.3:+61979069 None:intergenic
GAATCCAAAGGCATTTGAAG+TGG 0.606386 8.3:-61979092 MS.gene57526:CDS
TAAAGAATAATCCTTCTTTG+GGG 0.607481 8.3:+61979503 None:intergenic
GCATCTCAAAATGAATCCAA+AGG 0.611920 8.3:-61979104 MS.gene57526:CDS
TAAACTTGCTCACGGTCACG+AGG 0.626552 8.3:+61979040 None:intergenic
AGAGAAGAAATACACTCGCA+TGG 0.629992 8.3:-61980148 MS.gene57526:CDS
GGTGATATTATTGGAACCGG+TGG 0.633051 8.3:-61979172 MS.gene57526:CDS
TTGGTGAAGTATAATAACAA+AGG 0.635362 8.3:-61979139 MS.gene57526:CDS
TATGGACACAATTCTTGCGG+CGG 0.636506 8.3:+61979981 None:intergenic
AACCATATAGATCACTTGGA+CGG 0.636692 8.3:+61979683 None:intergenic
GGCGGAAACATCAACATGCG+AGG 0.638106 8.3:+61979999 None:intergenic
CCATACATGTCTTCTTCGCG+AGG 0.647704 8.3:-61979881 MS.gene57526:CDS
GCGGAAACATCAACATGCGA+GGG 0.648491 8.3:+61980000 None:intergenic
ACATTATCATGATATACGGG+TGG 0.671143 8.3:-61979455 MS.gene57526:CDS
ACCTTCAAATAAGTGAAGTG+GGG 0.674819 8.3:+61979526 None:intergenic
AAACTTGCTCACGGTCACGA+GGG 0.700858 8.3:+61979041 None:intergenic
GAGTATATAGGGGAAAACCA+AGG 0.732405 8.3:+61979205 None:intergenic
TAAGGTTGGAATTCGACACA+AGG 0.741160 8.3:+61979615 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGAATGATTAATTTAGA+AGG + chr8.3:61979096-61979115 None:intergenic 15.0%
!! AAAGAAGGATTATTCTTTAA+TGG - chr8.3:61979693-61979712 MS.gene57526:CDS 20.0%
!! AAGAAGGATTATTCTTTAAT+GGG - chr8.3:61979694-61979713 MS.gene57526:CDS 20.0%
!! ATTAAAGAATAATCCTTCTT+TGG + chr8.3:61979694-61979713 None:intergenic 20.0%
!! TTAAAGAATAATCCTTCTTT+GGG + chr8.3:61979693-61979712 None:intergenic 20.0%
!!! GAATATGAATTTTCATAATC+CGG - chr8.3:61979881-61979900 MS.gene57526:CDS 20.0%
!!! GCTTGATTTTTTTATGTTTA+CGG + chr8.3:61979412-61979431 None:intergenic 20.0%
!!! TATTGTTGTCTTTGATTTAA+TGG - chr8.3:61979764-61979783 MS.gene57526:intron 20.0%
! AATACAAACTGAAATCATCT+TGG - chr8.3:61979928-61979947 MS.gene57526:CDS 25.0%
! ACAAAAAGTGGTGATATTAT+TGG - chr8.3:61980011-61980030 MS.gene57526:CDS 25.0%
! AGAAGGATTATTCTTTAATG+GGG - chr8.3:61979695-61979714 MS.gene57526:CDS 25.0%
! CTTCACATTATCATGATATA+CGG - chr8.3:61979733-61979752 MS.gene57526:intron 25.0%
! TAAAGAATAATCCTTCTTTG+GGG + chr8.3:61979692-61979711 None:intergenic 25.0%
! TTCACATTATCATGATATAC+GGG - chr8.3:61979734-61979753 MS.gene57526:intron 25.0%
! TTGGTGAAGTATAATAACAA+AGG - chr8.3:61980053-61980072 MS.gene57526:CDS 25.0%
! TTTCATTGAGAGATAATACA+TGG - chr8.3:61979628-61979647 MS.gene57526:CDS 25.0%
!! ACTTTTTGTAGAGTATATAG+GGG + chr8.3:61980000-61980019 None:intergenic 25.0%
!! ATATGATGAAGTTTTCACTA+AGG + chr8.3:61979598-61979617 None:intergenic 25.0%
!! TTGTCTTTGATTTAATGGAA+AGG - chr8.3:61979769-61979788 MS.gene57526:intron 25.0%
!!! CACTTTTTGTAGAGTATATA+GGG + chr8.3:61980001-61980020 None:intergenic 25.0%
AAAAATCAAGCCTCTTTGTA+TGG - chr8.3:61979421-61979440 MS.gene57526:CDS 30.0%
AAACATAAAAGAGACTTCAC+CGG + chr8.3:61979132-61979151 None:intergenic 30.0%
AAATTAAAGGTTCATGCAGA+GGG - chr8.3:61979348-61979367 MS.gene57526:CDS 30.0%
AACCATAATACTTAGGATCA+TGG + chr8.3:61979830-61979849 None:intergenic 30.0%
AACCATGATCCTAAGTATTA+TGG - chr8.3:61979825-61979844 MS.gene57526:intron 30.0%
ATCCTAAGTATTATGGTTTG+TGG - chr8.3:61979832-61979851 MS.gene57526:intron 30.0%
CCTATATACTCTACAAAAAG+TGG - chr8.3:61979999-61980018 MS.gene57526:CDS 30.0%
TAGATAGATTCCATACAAAG+AGG + chr8.3:61979434-61979453 None:intergenic 30.0%
TGAAACAATATCATGGTTGA+TGG + chr8.3:61979286-61979305 None:intergenic 30.0%
! TATTATGGTTTGTGGGTATT+TGG - chr8.3:61979840-61979859 MS.gene57526:intron 30.0%
! TCCTAAGTATTATGGTTTGT+GGG - chr8.3:61979833-61979852 MS.gene57526:intron 30.0%
!!! CCACTTTTTGTAGAGTATAT+AGG + chr8.3:61980002-61980021 None:intergenic 30.0%
AACCATATAGATCACTTGGA+CGG + chr8.3:61979512-61979531 None:intergenic 35.0%
AAGCAGAGACTCTTTATACA+TGG + chr8.3:61980126-61980145 None:intergenic 35.0%
ACATTATCATGATATACGGG+TGG - chr8.3:61979737-61979756 MS.gene57526:intron 35.0%
ACCCACAAACCATAATACTT+AGG + chr8.3:61979837-61979856 None:intergenic 35.0%
ACCTTCAAATAAGTGAAGTG+GGG + chr8.3:61979669-61979688 None:intergenic 35.0%
AGTGGTGATATTATTGGAAC+CGG - chr8.3:61980017-61980036 MS.gene57526:CDS 35.0%
ATAATCCGGCACTTGAAATA+TGG - chr8.3:61979895-61979914 MS.gene57526:CDS 35.0%
ATCAAGAGATGATTACTTGG+TGG - chr8.3:61979545-61979564 MS.gene57526:CDS 35.0%
CAACCATGATATTGTTTCAC+TGG - chr8.3:61979287-61979306 MS.gene57526:CDS 35.0%
CAAGGTATGTCATTGTCAAT+AGG + chr8.3:61979972-61979991 None:intergenic 35.0%
CCAAAACCATATAGATCACT+TGG + chr8.3:61979516-61979535 None:intergenic 35.0%
CCAAGTAATCATCTCTTGAT+CGG + chr8.3:61979545-61979564 None:intergenic 35.0%
CCATGTAATTCTGCAGATAA+AGG + chr8.3:61979486-61979505 None:intergenic 35.0%
CCTTTATCTGCAGAATTACA+TGG - chr8.3:61979483-61979502 MS.gene57526:CDS 35.0%
CTATTGACAATGACATACCT+TGG - chr8.3:61979970-61979989 MS.gene57526:CDS 35.0%
GAAATTAAAGGTTCATGCAG+AGG - chr8.3:61979347-61979366 MS.gene57526:CDS 35.0%
GACCTTCAAATAAGTGAAGT+GGG + chr8.3:61979670-61979689 None:intergenic 35.0%
GCATCTCAAAATGAATCCAA+AGG - chr8.3:61980088-61980107 MS.gene57526:CDS 35.0%
TAATCATTCAAATCCTGCTG+AGG - chr8.3:61979103-61979122 MS.gene57526:CDS 35.0%
TAATCCGGCACTTGAAATAT+GGG - chr8.3:61979896-61979915 MS.gene57526:CDS 35.0%
TCAAATGCGTTTGTAAGTCA+TGG - chr8.3:61979151-61979170 MS.gene57526:CDS 35.0%
TCTTTAATGGGGCTATTCAT+TGG - chr8.3:61979706-61979725 MS.gene57526:intron 35.0%
TGATATGGACACAATTCTTG+CGG + chr8.3:61979217-61979236 None:intergenic 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr8.3:61979859-61979878 MS.gene57526:CDS 35.0%
! CCAAGTGATCTATATGGTTT+TGG - chr8.3:61979513-61979532 MS.gene57526:CDS 35.0%
! GATGAAGTTTTCACTAAGGT+TGG + chr8.3:61979594-61979613 None:intergenic 35.0%
AGAGAAGAAATACACTCGCA+TGG - chr8.3:61979044-61979063 MS.gene57526:CDS 40.0%
ATAGAGCGAATTTCAGCAAG+AGG + chr8.3:61979249-61979268 None:intergenic 40.0%
CCGATCAAGAGATGATTACT+TGG - chr8.3:61979542-61979561 MS.gene57526:CDS 40.0%
GAGTATATAGGGGAAAACCA+AGG + chr8.3:61979990-61980009 None:intergenic 40.0%
GGACCTTCAAATAAGTGAAG+TGG + chr8.3:61979671-61979690 None:intergenic 40.0%
GGATCATGGTTGAAATCATC+TGG + chr8.3:61979816-61979835 None:intergenic 40.0%
GGTCCAGTGAAACAATATCA+TGG + chr8.3:61979293-61979312 None:intergenic 40.0%
TAAGGTTGGAATTCGACACA+AGG + chr8.3:61979580-61979599 None:intergenic 40.0%
TAATGGGGCTATTCATTGGT+TGG - chr8.3:61979710-61979729 MS.gene57526:intron 40.0%
TACCGTCCAAGTGATCTATA+TGG - chr8.3:61979507-61979526 MS.gene57526:CDS 40.0%
TATTTGAAGGTCCCCAAAGA+AGG - chr8.3:61979678-61979697 MS.gene57526:CDS 40.0%
TCCCCACTTCACTTATTTGA+AGG - chr8.3:61979665-61979684 MS.gene57526:CDS 40.0%
TCTGCAGATAAAGGAGACAA+AGG + chr8.3:61979477-61979496 None:intergenic 40.0%
TGGTTGAAATCATCTGGAAG+AGG + chr8.3:61979810-61979829 None:intergenic 40.0%
! ATCACCCATATTTCAAGTGC+CGG + chr8.3:61979903-61979922 None:intergenic 40.0%
!! AAGAGGTCGAGGTTTTGATA+TGG + chr8.3:61979232-61979251 None:intergenic 40.0%
!! GAATCCAAAGGCATTTGAAG+TGG - chr8.3:61980100-61980119 MS.gene57526:CDS 40.0%
ATGGCCACTTCAAATGCCTT+TGG + chr8.3:61980107-61980126 None:intergenic 45.0%
GGTGATATTATTGGAACCGG+TGG - chr8.3:61980020-61980039 MS.gene57526:CDS 45.0%
TATGGACACAATTCTTGCGG+CGG + chr8.3:61979214-61979233 None:intergenic 45.0%
!!! TAATTTTTTTATGAAATTAA+AGG - chr8.3:61979335-61979354 MS.gene57526:CDS 5.0%
CCATACATGTCTTCTTCGCG+AGG - chr8.3:61979311-61979330 MS.gene57526:CDS 50.0%
CCTCGCGAAGAAGACATGTA+TGG + chr8.3:61979314-61979333 None:intergenic 50.0%
CGAATTTCAGCAAGAGGTCG+AGG + chr8.3:61979243-61979262 None:intergenic 50.0%
GCGGAAACATCAACATGCGA+GGG + chr8.3:61979195-61979214 None:intergenic 50.0%
TCAAATCCTGCTGAGGTTGC+CGG - chr8.3:61979110-61979129 MS.gene57526:CDS 50.0%
! AAACTTGCTCACGGTCACGA+GGG + chr8.3:61980154-61980173 None:intergenic 50.0%
! TAAACTTGCTCACGGTCACG+AGG + chr8.3:61980155-61980174 None:intergenic 50.0%
AACCGGTGGTCGTCATGGAT+TGG - chr8.3:61980034-61980053 MS.gene57526:CDS 55.0%
ATTGGAACCGGTGGTCGTCA+TGG - chr8.3:61980029-61980048 MS.gene57526:CDS 55.0%
CACCAATCCATGACGACCAC+CGG + chr8.3:61980039-61980058 None:intergenic 55.0%
GGCGGAAACATCAACATGCG+AGG + chr8.3:61979196-61979215 None:intergenic 55.0%
ACTTCACCGGCAACCTCAGC+AGG + chr8.3:61979119-61979138 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 61979039 61980175 61979039 ID=MS.gene57526
chr8.3 mRNA 61979039 61980175 61979039 ID=MS.gene57526.t1;Parent=MS.gene57526
chr8.3 exon 61979845 61980175 61979845 ID=MS.gene57526.t1.exon1;Parent=MS.gene57526.t1
chr8.3 CDS 61979845 61980175 61979845 ID=cds.MS.gene57526.t1;Parent=MS.gene57526.t1
chr8.3 exon 61979039 61979718 61979039 ID=MS.gene57526.t1.exon2;Parent=MS.gene57526.t1
chr8.3 CDS 61979039 61979718 61979039 ID=cds.MS.gene57526.t1;Parent=MS.gene57526.t1
Gene Sequence

>MS.gene57526

ATGGCAGAGAAGAAATACACTCGCATGGCGAAGATGAAAGAGACGTTATATCTTCCTTCTAAATTAATCATTCAAATCCTGCTGAGGTTGCCGGTGAAGTCTCTTTTATGTTTCAAATGCGTTTGTAAGTCATGGTTTTCTCTTATCTCTGATCCCTCGCATGTTGATGTTTCCGCCGCAAGAATTGTGTCCATATCAAAACCTCGACCTCTTGCTGAAATTCGCTCTATAGATTTTGAAACATCCATCAACCATGATATTGTTTCACTGGACCATACATGTCTTCTTCGCGAGGATAATTTTTTTATGAAATTAAAGGTTCATGCAGAGGGTTTATAATTTTGCGAGATTTCACAAACGACTATGTATTCCGTAAACATAAAAAAATCAAGCCTCTTTGTATGGAATCTATCTACTAGATTTCACAAAGAAATACCTTTGTCTCCTTTATCTGCAGAATTACATGGATACCGTCCAAGTGATCTATATGGTTTTGGTTATGACCGATCAAGAGATGATTACTTGGTGGTTTTATTGTCCTTGTGTCGAATTCCAACCTTAGTGAAAACTTCATCATATTTAGAGTTTTTTTCATTGAGAGATAATACATGGAATGAAATTAAAGATCCCCACTTCACTTATTTGAAGGTCCCCAAAGAAGGATTATTCTTTAATGGGGCTATTCATTGGTTGGCTTCACATTATCATGATATACGGGTGGAAGTTATTGTTGTCTTTGATTTAATGGAAAGGAAACTGTTAGAGATACCTCTTCCAGATGATTTCAACCATGATCCTAAGTATTATGGTTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGAATATGAATTTTCATAATCCGGCACTTGAAATATGGGTGATGAAAGAATACAAACTGAAATCATCTTGGACTAAGACTCTTGTAATTCCTATTGACAATGACATACCTTGGTTTTCCCCTATATACTCTACAAAAAGTGGTGATATTATTGGAACCGGTGGTCGTCATGGATTGGTGAAGTATAATAACAAAGGACAACTTTTAGAGCATCTCAAAATGAATCCAAAGGCATTTGAAGTGGCCATGTATAAAGAGTCTCTGCTTTCACTCCCTCGTGACCGTGAGCAAGTTTAA
Protein sequence

>MS.gene57526.t1

MAEKKYTRMAKMKETLYLPSKLIIQILLRLPVKSLLCFKCVCKSWFSLISDPSHVDVSAARIVSISKPRPLAEIRSIDFETSINHDIVSLDHTCLLREDNFFMKLKVHAEELHGYRPSDLYGFGYDRSRDDYLVVLLSLCRIPTLVKTSSYLEFFSLRDNTWNEIKDPHFTYLKVPKEGLFFNGAIHWLASHYHDIRVEVIVVFDLMERKLLEIPLPDDFNHDPKYYGLWVFGEFLSLWNMNFHNPALEIWVMKEYKLKSSWTKTLVIPIDNDIPWFSPIYSTKSGDIIGTGGRHGLVKYNNKGQLLEHLKMNPKAFEVAMYKESLLSLPRDREQV