AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034680.t1 MTR_1g055135 69.254 335 89 4 3 331 49 375 1.20e-155 441
MS.gene034680.t1 MTR_1g055195 55.385 325 113 7 24 331 169 478 3.51e-108 324
MS.gene034680.t1 MTR_1g055165 54.518 332 89 6 3 331 46 318 8.07e-108 317
MS.gene034680.t1 MTR_5g097210 53.354 328 138 8 3 328 46 360 3.26e-103 308
MS.gene034680.t1 MTR_4g117750 50.595 336 141 7 3 330 40 358 1.12e-94 290
MS.gene034680.t1 MTR_4g117750 50.909 110 50 3 223 329 373 481 1.40e-23 101
MS.gene034680.t1 MTR_1g055175 70.103 194 56 2 140 331 8 201 2.51e-91 271
MS.gene034680.t1 MTR_3g103020 49.408 338 147 10 3 331 56 378 5.08e-90 274
MS.gene034680.t1 MTR_4g117770 47.041 338 153 9 3 331 47 367 4.66e-88 269
MS.gene034680.t1 MTR_4g117810 47.041 338 153 9 3 331 47 367 4.66e-88 269
MS.gene034680.t1 MTR_5g070130 45.797 345 157 12 3 331 47 377 1.24e-85 263
MS.gene034680.t1 MTR_4g118370 56.504 246 84 5 84 328 34 257 1.53e-84 256
MS.gene034680.t1 MTR_4g023080 46.726 336 144 10 3 330 45 353 2.14e-82 254
MS.gene034680.t1 MTR_4g109270 48.071 337 150 12 3 331 45 364 2.23e-82 254
MS.gene034680.t1 MTR_4g117850 48.328 329 148 11 3 324 60 373 1.24e-81 253
MS.gene034680.t1 MTR_5g070088 44.571 350 162 7 3 329 43 383 2.89e-81 252
MS.gene034680.t1 MTR_4g117720 48.466 326 143 12 18 331 66 378 4.01e-81 251
MS.gene034680.t1 MTR_3g034180 45.294 340 164 11 3 330 159 488 5.80e-80 252
MS.gene034680.t1 MTR_4g117780 44.510 337 161 10 3 330 47 366 8.55e-80 248
MS.gene034680.t1 MTR_4g122850 45.217 345 156 13 3 331 37 364 4.18e-79 246
MS.gene034680.t1 MTR_5g070080 43.820 356 158 12 3 331 46 386 9.43e-79 245
MS.gene034680.t1 MTR_4g109280 49.254 335 145 14 3 331 44 359 6.18e-78 243
MS.gene034680.t1 MTR_3g034130 45.872 327 156 10 3 317 45 362 1.38e-76 239
MS.gene034680.t1 MTR_6g060650 45.821 347 151 13 3 330 44 372 1.10e-74 234
MS.gene034680.t1 MTR_4g023090 42.135 356 156 12 3 330 52 385 3.08e-74 234
MS.gene034680.t1 MTR_4g023070 43.516 347 161 11 3 330 39 369 6.83e-74 233
MS.gene034680.t1 MTR_4g094365 44.214 337 164 10 3 331 44 364 1.32e-73 231
MS.gene034680.t1 MTR_3g034160 44.868 341 164 9 3 330 45 374 2.63e-73 231
MS.gene034680.t1 MTR_4g023150 43.750 336 157 12 3 329 46 358 2.93e-73 230
MS.gene034680.t1 MTR_1g055250 62.319 207 73 4 107 311 46 249 3.55e-73 229
MS.gene034680.t1 MTR_4g021785 43.068 339 155 10 3 331 45 355 1.28e-72 229
MS.gene034680.t1 MTR_4g023190 43.750 336 156 13 3 329 46 357 1.82e-72 228
MS.gene034680.t1 MTR_4g116330 42.857 336 171 12 3 331 59 380 2.32e-72 229
MS.gene034680.t1 MTR_4g023380 40.548 365 144 12 3 331 52 379 7.54e-72 228
MS.gene034680.t1 MTR_4g023120 41.408 355 159 12 3 331 39 370 3.61e-71 225
MS.gene034680.t1 MTR_4g023200 43.155 336 159 12 3 329 46 358 4.60e-71 225
MS.gene034680.t1 MTR_4g023130 46.154 299 132 8 40 330 35 312 4.16e-69 218
MS.gene034680.t1 MTR_4g118380 43.385 325 145 12 3 301 44 355 5.94e-67 214
MS.gene034680.t1 MTR_8g433040 40.588 340 162 13 3 331 54 364 1.22e-60 198
MS.gene034680.t1 MTR_8g032380 40.588 340 162 13 3 331 54 364 1.22e-60 198
MS.gene034680.t1 MTR_4g023440 38.988 336 139 10 3 330 54 331 5.33e-60 196
MS.gene034680.t1 MTR_4g117870 39.130 299 139 7 35 331 10 267 1.08e-58 190
MS.gene034680.t1 MTR_7g079400 42.336 274 148 5 65 331 67 337 1.49e-56 187
MS.gene034680.t1 MTR_4g118400 44.000 275 113 14 57 325 55 294 4.74e-55 181
MS.gene034680.t1 MTR_4g116330 38.438 333 151 12 3 331 59 341 7.27e-55 182
MS.gene034680.t1 MTR_7g079370 37.798 336 190 7 3 326 23 351 1.00e-53 180
MS.gene034680.t1 MTR_8g032390 46.863 271 116 14 69 325 113 369 3.54e-53 179
MS.gene034680.t1 MTR_4g116320 47.465 217 107 5 115 328 43 255 1.36e-51 171
MS.gene034680.t1 MTR_8g433030 50.000 234 92 13 103 325 117 336 3.54e-48 165
MS.gene034680.t1 MTR_8g102520 36.716 335 169 10 3 331 25 322 4.54e-47 162
MS.gene034680.t1 MTR_8g013010 43.882 237 109 8 3 232 90 309 7.27e-45 155
MS.gene034680.t1 MTR_8g040935 46.829 205 97 7 135 331 8 208 1.79e-43 149
MS.gene034680.t1 MTR_4g121580 36.686 338 164 17 4 327 48 349 1.20e-40 146
MS.gene034680.t1 MTR_6g463430 46.500 200 93 9 137 331 12 202 1.67e-40 141
MS.gene034680.t1 MTR_4g117530 41.333 225 86 7 109 331 73 253 1.12e-38 138
MS.gene034680.t1 MTR_2g048580 32.934 334 184 14 3 331 44 342 1.39e-37 137
MS.gene034680.t1 MTR_7g079420 35.842 279 123 10 70 331 905 1144 1.36e-35 137
MS.gene034680.t1 MTR_4g118360 56.140 114 48 2 219 331 14 126 1.40e-35 125
MS.gene034680.t1 MTR_4g114700 35.417 240 97 9 67 299 5 193 3.51e-31 117
MS.gene034680.t1 MTR_5g023000 61.053 95 34 2 239 331 6 99 1.56e-29 109
MS.gene034680.t1 MTR_4g117430 42.945 163 79 4 71 232 3 152 1.83e-28 108
MS.gene034680.t1 MTR_1g055200 63.855 83 28 1 67 149 5 85 4.69e-28 105
MS.gene034680.t1 MTR_7g117720 39.623 159 85 6 9 162 32 184 2.24e-27 108
MS.gene034680.t1 MTR_7g117720 33.540 161 92 4 171 326 122 272 4.01e-17 80.5
MS.gene034680.t1 MTR_7g079450 41.606 137 78 2 195 331 3 137 5.17e-25 98.6
MS.gene034680.t1 MTR_1g055145 62.195 82 31 0 167 248 121 202 3.09e-23 97.4
MS.gene034680.t1 MTR_1g055145 77.612 67 10 1 3 69 69 130 2.17e-22 95.1
MS.gene034680.t1 MTR_4g122860 35.751 193 88 8 145 330 156 319 7.61e-23 97.4
MS.gene034680.t1 MTR_8g098830 37.349 166 97 4 143 305 37 198 2.17e-22 94.0
MS.gene034680.t1 MTR_4g116340 42.069 145 64 5 52 186 100 234 8.74e-22 92.8
MS.gene034680.t1 MTR_1g068835 30.035 283 154 13 42 297 67 332 1.17e-20 92.0
MS.gene034680.t1 MTR_4g133170 29.114 237 142 8 63 281 85 313 1.41e-20 92.0
MS.gene034680.t1 MTR_2g026010 30.952 210 127 6 68 261 90 297 3.75e-20 90.9
MS.gene034680.t1 MTR_3g013740 29.603 277 163 15 3 261 80 342 3.69e-19 87.8
MS.gene034680.t1 MTR_5g069120 40.127 157 78 6 3 149 27 177 5.62e-19 83.6
MS.gene034680.t1 MTR_4g061340 31.707 246 134 11 33 261 87 315 1.39e-18 85.9
MS.gene034680.t1 MTR_2g025420 29.204 226 119 8 68 261 106 322 1.40e-18 86.3
MS.gene034680.t1 MTR_5g057790 30.579 242 133 11 34 261 110 330 1.77e-18 85.9
MS.gene034680.t1 MTR_0667s0010 28.862 246 140 9 42 261 70 306 5.88e-18 84.3
MS.gene034680.t1 MTR_4g070550 30.667 225 115 9 64 257 97 311 9.38e-18 84.0
MS.gene034680.t1 MTR_3g083010 27.602 221 128 6 68 262 108 322 5.71e-17 81.6
MS.gene034680.t1 MTR_2g028130 28.916 249 145 9 36 261 76 315 8.12e-17 81.3
MS.gene034680.t1 MTR_2g031680 29.134 254 153 7 64 297 87 333 9.37e-17 80.9
MS.gene034680.t1 MTR_8g099460 29.864 221 126 8 63 261 88 301 1.04e-16 80.9
MS.gene034680.t1 MTR_2g021050 29.412 204 121 6 68 261 95 285 1.46e-16 80.1
MS.gene034680.t1 MTR_6g034720 42.623 122 60 3 94 210 53 169 2.88e-16 77.0
MS.gene034680.t1 MTR_2g028140 27.798 277 151 11 68 312 107 366 6.78e-16 78.6
MS.gene034680.t1 MTR_6g021880 27.715 267 171 10 3 257 56 312 3.00e-15 76.3
MS.gene034680.t1 MTR_2g024100 27.397 219 122 6 68 257 96 306 3.08e-15 76.3
MS.gene034680.t1 MTR_5g057710 30.738 244 130 13 34 261 110 330 3.48e-15 76.3
MS.gene034680.t1 MTR_4g055060 31.557 244 137 16 33 261 93 321 4.19e-15 75.9
MS.gene034680.t1 MTR_2g031640 28.571 210 128 6 68 261 92 295 4.69e-15 75.9
MS.gene034680.t1 MTR_3g014570 27.509 269 157 12 9 261 69 315 5.26e-15 75.5
MS.gene034680.t1 MTR_3g009310 30.085 236 133 10 33 257 108 322 5.76e-15 75.5
MS.gene034680.t1 MTR_1g068860 29.536 237 140 11 42 261 67 293 6.42e-15 75.1
MS.gene034680.t1 MTR_3g014540 28.676 272 150 15 9 261 69 315 6.65e-15 75.1
MS.gene034680.t1 MTR_1g068875 25.417 240 143 9 42 261 67 290 7.71e-15 75.1
MS.gene034680.t1 MTR_2g031720 29.048 210 127 6 68 261 96 299 8.55e-15 75.1
MS.gene034680.t1 MTR_2g028160 29.032 217 126 7 68 261 106 317 9.12e-15 75.1
MS.gene034680.t1 MTR_7g070440 29.130 230 124 12 65 267 94 311 9.75e-15 74.7
MS.gene034680.t1 MTR_5g097680 29.817 218 116 9 77 261 152 365 1.26e-14 74.7
MS.gene034680.t1 MTR_2g426090 25.524 286 169 11 3 261 42 310 1.35e-14 74.3
MS.gene034680.t1 MTR_3g006670 28.767 219 122 11 68 273 146 343 1.71e-14 74.3
MS.gene034680.t1 MTR_2g028110 28.829 222 126 9 63 261 85 297 1.72e-14 73.9
MS.gene034680.t1 MTR_3g007360 27.138 269 146 13 33 277 112 354 2.12e-14 73.9
MS.gene034680.t1 MTR_3g007410 26.792 265 152 12 33 277 112 354 3.34e-14 73.2
MS.gene034680.t1 MTR_2g425980 27.881 269 148 9 23 261 60 312 5.12e-14 72.8
MS.gene034680.t1 MTR_3g024130 28.571 245 140 12 33 261 25 250 6.21e-14 72.0
MS.gene034680.t1 MTR_5g057020 27.969 261 149 12 3 256 82 310 1.29e-13 71.2
MS.gene034680.t1 MTR_2g024110 26.293 232 132 9 50 262 83 294 2.64e-13 70.5
MS.gene034680.t1 MTR_3g036290 28.626 262 150 11 3 257 58 289 3.73e-13 69.7
MS.gene034680.t1 MTR_4g023430 46.341 82 40 2 254 331 141 222 3.98e-13 68.2
MS.gene034680.t1 MTR_3g009300 29.249 253 133 13 33 269 69 291 4.04e-13 69.7
MS.gene034680.t1 MTR_8g013005 52.459 61 26 2 272 331 1 59 4.34e-13 63.5
MS.gene034680.t1 MTR_1g094210 25.449 334 208 11 3 323 93 398 4.44e-13 69.7
MS.gene034680.t1 MTR_5g046280 30.337 178 107 7 82 257 20 182 5.32e-13 68.2
MS.gene034680.t1 MTR_5g046300 30.337 178 107 7 82 257 117 279 5.74e-13 69.3
MS.gene034680.t1 MTR_5g046290 30.337 178 107 7 82 257 117 279 5.74e-13 69.3
MS.gene034680.t1 MTR_3g007430 28.980 245 139 12 33 261 103 328 8.33e-13 68.9
MS.gene034680.t1 MTR_1g094210 25.552 317 197 10 3 306 93 383 1.35e-12 68.6
MS.gene034680.t1 MTR_3g013770 30.288 208 113 11 68 261 134 323 1.38e-12 68.2
MS.gene034680.t1 MTR_7g013590 29.907 214 133 7 54 257 114 320 1.47e-12 68.2
MS.gene034680.t1 MTR_2g021155 30.037 273 154 14 3 261 72 321 1.47e-12 68.9
MS.gene034680.t1 MTR_2g021155 30.978 184 105 10 84 257 711 882 7.74e-11 63.5
MS.gene034680.t1 MTR_2g104340 26.871 294 164 14 72 328 117 396 1.50e-12 68.2
MS.gene034680.t1 MTR_3g009240 29.614 233 140 10 33 257 96 312 1.59e-12 68.2
MS.gene034680.t1 MTR_3g023930 27.027 259 151 12 3 257 70 294 1.65e-12 68.2
MS.gene034680.t1 MTR_1g054725 26.609 233 148 7 35 257 87 306 2.26e-12 67.8
MS.gene034680.t1 MTR_5g009060 27.193 228 130 10 54 262 133 343 2.56e-12 67.4
MS.gene034680.t1 MTR_2g104280 26.117 291 153 13 82 329 135 406 2.70e-12 67.4
MS.gene034680.t1 MTR_2g021770 28.102 274 161 12 84 328 141 407 2.87e-12 67.4
MS.gene034680.t1 MTR_4g014370 25.087 287 175 14 70 324 127 405 3.97e-12 67.0
MS.gene034680.t1 MTR_2g029990 30.151 199 116 10 68 257 150 334 4.62e-12 67.0
MS.gene034680.t1 MTR_2g038680 24.286 280 167 10 80 324 138 407 3.51e-11 64.3
MS.gene034680.t1 MTR_2g046900 26.908 249 163 10 28 268 89 326 3.71e-11 63.9
MS.gene034680.t1 MTR_3g030250 23.774 265 169 11 3 261 73 310 4.12e-11 63.9
MS.gene034680.t1 MTR_3g011020 31.288 163 87 9 110 261 157 305 4.17e-11 63.9
MS.gene034680.t1 MTR_1g097560 24.324 259 166 9 47 293 106 346 4.88e-11 63.5
MS.gene034680.t1 MTR_7g070880 23.885 314 178 13 66 324 114 421 5.54e-11 63.5
MS.gene034680.t1 MTR_2g021252 26.562 256 148 13 13 257 90 316 5.69e-11 63.5
MS.gene034680.t1 MTR_3g011060 30.189 212 109 14 64 261 138 324 6.50e-11 63.2
MS.gene034680.t1 MTR_5g046260 28.249 177 99 7 81 257 101 249 9.22e-11 62.4
MS.gene034680.t1 MTR_3g011100 29.817 218 125 12 52 257 320 521 9.89e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034680.t1 AT4G12560 32.342 269 153 14 14 261 43 303 7.60e-18 84.3
MS.gene034680.t1 AT4G12560 32.342 269 153 14 14 261 43 303 7.60e-18 84.3
MS.gene034680.t1 AT4G12560 32.342 269 153 14 14 261 43 303 7.60e-18 84.3
MS.gene034680.t1 AT3G06240 26.523 279 164 12 64 323 163 419 4.06e-15 76.3
MS.gene034680.t1 AT3G23880 26.667 240 158 8 25 259 73 299 1.98e-13 70.9
MS.gene034680.t1 AT4G22390 24.000 325 208 13 2 299 31 343 8.60e-12 66.2
MS.gene034680.t1 AT4G22390 24.000 325 208 13 2 299 31 343 8.90e-12 65.9
MS.gene034680.t1 AT3G07870 27.636 275 150 12 66 298 119 386 2.90e-11 64.3

Find 73 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTGAAAATTTGTAGTTTA+TGG 0.101383 1.4:+46432031 MS.gene034680:CDS
TGTTGTTGCCTTTGATTTAA+TGG 0.147144 1.4:+46431966 MS.gene034680:CDS
TTAAGATGAAACTCAGATTT+AGG 0.217156 1.4:-46431553 None:intergenic
TTGGCTATTTGTCCTGTTAC+AGG 0.219966 1.4:+46431934 MS.gene034680:CDS
ACGAGGATAAAGTTGATATA+TGG 0.231752 1.4:+46432091 MS.gene034680:CDS
ATTTCTCAGTCTATGGGTTT+GGG 0.250885 1.4:+46432065 MS.gene034680:CDS
TAAAACTCTTGTTCTTGATT+TGG 0.263623 1.4:+46432149 MS.gene034680:CDS
GATTTCTCAGTCTATGGGTT+TGG 0.272519 1.4:+46432064 MS.gene034680:CDS
TTAGAGGATTTCTCAGTCTA+TGG 0.277856 1.4:+46432058 MS.gene034680:CDS
TTTGAAAATTTGTAGTTTAT+GGG 0.289443 1.4:+46432032 MS.gene034680:CDS
GAGTTTCATCTTAAAGTTAG+AGG 0.318745 1.4:+46431562 MS.gene034680:CDS
TCATACCCAAAGCCATAAAA+AGG 0.326987 1.4:-46431703 None:intergenic
GATGACAAAGGAGAGTTTCT+AGG 0.338641 1.4:+46432258 MS.gene034680:CDS
TTCAAACGACCAATATCATA+TGG 0.346728 1.4:-46432015 None:intergenic
TTCATGTAGAGGGTTTATAT+TGG 0.349285 1.4:+46431585 MS.gene034680:CDS
TGAGCAAGATTATCACAAAA+AGG 0.360361 1.4:-46431862 None:intergenic
TGTAGTTTATGGGTATTTAG+AGG 0.364817 1.4:+46432042 MS.gene034680:CDS
TTAAGCTTGCTCATTGTCAC+TGG 0.372258 1.4:-46432355 None:intergenic
TGGTAGAGTCGGTCTTCAAT+GGG 0.399762 1.4:+46431899 MS.gene034680:CDS
ATTCCTCTCCCATATGATAT+TGG 0.400405 1.4:+46432006 MS.gene034680:CDS
ACAAGATGGTCACCTGTAAC+AGG 0.408447 1.4:-46431946 None:intergenic
AAAGGTATACTTGAATGATT+AGG 0.418298 1.4:-46431685 None:intergenic
CGAGGATAAAGTTGATATAT+GGG 0.422656 1.4:+46432092 MS.gene034680:CDS
TCTTCAATGGGGCTATTCAC+TGG 0.429378 1.4:+46431911 MS.gene034680:CDS
GTGGTAGAGTCGGTCTTCAA+TGG 0.432995 1.4:+46431898 MS.gene034680:CDS
AATACAAAGTGAAGTCATCT+TGG 0.433399 1.4:+46432124 MS.gene034680:CDS
AAATTAGGGTTCAGTAGAAC+AGG 0.443041 1.4:-46431523 None:intergenic
ATGTAGAGGGTTTATATTGG+TGG 0.455379 1.4:+46431588 MS.gene034680:CDS
GGATTCCATAAACACATGAT+TGG 0.457528 1.4:-46431619 None:intergenic
TACAAGGGAAATGTGAGAAA+TGG 0.461687 1.4:-46432175 None:intergenic
CGAGTCAACAGATGATTACT+TGG 0.478220 1.4:+46431726 MS.gene034680:CDS
CTTTCTTTCCATTAAATCAA+AGG 0.482406 1.4:-46431974 None:intergenic
CAATGGGGCTATTCACTGGT+TGG 0.483990 1.4:+46431915 MS.gene034680:CDS
TAAGCTTGCTCATTGTCACT+GGG 0.485254 1.4:-46432354 None:intergenic
TAGAGGATTTCTCAGTCTAT+GGG 0.489737 1.4:+46432059 MS.gene034680:CDS
ATGATGTGCTATCTCACATT+GGG 0.491270 1.4:+46431785 MS.gene034680:CDS
TGCTTATACTGTGGTAGAGT+CGG 0.504361 1.4:+46431888 MS.gene034680:CDS
ACGAAAAGTGGTGATATTGT+TGG 0.511464 1.4:+46432204 MS.gene034680:CDS
ATGACAAAGGAGAGTTTCTA+GGG 0.519234 1.4:+46432259 MS.gene034680:CDS
TTTCTCAGTCTATGGGTTTG+GGG 0.519639 1.4:+46432066 MS.gene034680:CDS
TCAAACGACCAATATCATAT+GGG 0.522119 1.4:-46432014 None:intergenic
AAATACCTTCGCCTCCGTCT+TGG 0.525982 1.4:+46431662 MS.gene034680:CDS
TTCTCAGTCTATGGGTTTGG+GGG 0.526208 1.4:+46432067 MS.gene034680:CDS
TGCTCACTTTGCTTATACTG+TGG 0.526532 1.4:+46431879 MS.gene034680:CDS
AAGTTAGAGGTTCATGTAGA+GGG 0.527722 1.4:+46431575 MS.gene034680:CDS
CTTGTATGTTGTACGAAAAG+TGG 0.529124 1.4:+46432192 MS.gene034680:CDS
AAAGCTATAGATCGAAATTG+AGG 0.533439 1.4:-46431472 None:intergenic
TGTCTATGAACTCCGGTGGA+TGG 0.541552 1.4:-46431640 None:intergenic
GTCAACAGATGATTACTTGG+TGG 0.548203 1.4:+46431729 MS.gene034680:CDS
AAAGTTAGAGGTTCATGTAG+AGG 0.561749 1.4:+46431574 MS.gene034680:CDS
TAATGTGTGGAACGAAATTG+TGG 0.563473 1.4:+46431825 MS.gene034680:CDS
GATGATGTGCTATCTCACAT+TGG 0.571631 1.4:+46431784 MS.gene034680:CDS
TCATATCTCACCAACCCACT+AGG 0.582987 1.4:-46432237 None:intergenic
TCTCTTTGAGAGCTAATGTG+TGG 0.587579 1.4:+46431812 MS.gene034680:CDS
AATCAAAGGCAACAACAAGA+TGG 0.590227 1.4:-46431960 None:intergenic
CTTGAATGATTAGGCCAAGA+CGG 0.590727 1.4:-46431676 None:intergenic
CGACCAATATCATATGGGAG+AGG 0.597534 1.4:-46432009 None:intergenic
TTCACAACATGCATTCCGAC+TGG 0.604293 1.4:+46432302 MS.gene034680:CDS
TTAGGCCAAGACGGAGGCGA+AGG 0.605451 1.4:-46431667 None:intergenic
TGTTTATGGAATCCATCCAC+CGG 0.612831 1.4:+46431628 MS.gene034680:CDS
AACAAATAGACCTAGTGGGT+TGG 0.613192 1.4:+46432227 MS.gene034680:CDS
AGGTATTTGTCTATGAACTC+CGG 0.618231 1.4:-46431647 None:intergenic
TCTCAGTCTATGGGTTTGGG+GGG 0.619882 1.4:+46432068 MS.gene034680:CDS
TAGAACAGGAGCAGAATCAG+CGG 0.621200 1.4:-46431509 None:intergenic
GTTGGAACAAATAGACCTAG+TGG 0.630759 1.4:+46432222 MS.gene034680:CDS
TATTTGTCTATGAACTCCGG+TGG 0.633571 1.4:-46431644 None:intergenic
TTGGAACAAATAGACCTAGT+GGG 0.639900 1.4:+46432223 MS.gene034680:CDS
TCTCTGTATACATAGCCAGT+CGG 0.674489 1.4:-46432317 None:intergenic
AAGCTTGCTCATTGTCACTG+GGG 0.678198 1.4:-46432353 None:intergenic
GGTAGAGTCGGTCTTCAATG+GGG 0.689885 1.4:+46431900 MS.gene034680:CDS
AATGTGTGGAACGAAATTGT+GGG 0.717821 1.4:+46431826 MS.gene034680:CDS
GAATGATTAGGCCAAGACGG+AGG 0.740832 1.4:-46431673 None:intergenic
TTGGTGAGATATGATGACAA+AGG 0.758777 1.4:+46432246 MS.gene034680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGAAAATTTGTAGTTTAT+GGG + chr1.4:46432032-46432051 MS.gene034680:CDS 15.0%
!! GTTTGAAAATTTGTAGTTTA+TGG + chr1.4:46432031-46432050 MS.gene034680:CDS 20.0%
! ATTTAGGAAGCGAAAAATTA+GGG - chr1.4:46431540-46431559 None:intergenic 25.0%
! CTTTCTTTCCATTAAATCAA+AGG - chr1.4:46431977-46431996 None:intergenic 25.0%
! TAAAACTCTTGTTCTTGATT+TGG + chr1.4:46432149-46432168 MS.gene034680:CDS 25.0%
! TTAAGATGAAACTCAGATTT+AGG - chr1.4:46431556-46431575 None:intergenic 25.0%
!! AAAGGTATACTTGAATGATT+AGG - chr1.4:46431688-46431707 None:intergenic 25.0%
!! CATTCAAGTATACCTTTTTA+TGG + chr1.4:46431691-46431710 MS.gene034680:CDS 25.0%
AAAGCTATAGATCGAAATTG+AGG - chr1.4:46431475-46431494 None:intergenic 30.0%
AATACAAAGTGAAGTCATCT+TGG + chr1.4:46432124-46432143 MS.gene034680:CDS 30.0%
GAGTTTCATCTTAAAGTTAG+AGG + chr1.4:46431562-46431581 MS.gene034680:CDS 30.0%
GATTTAGGAAGCGAAAAATT+AGG - chr1.4:46431541-46431560 None:intergenic 30.0%
TCAAACGACCAATATCATAT+GGG - chr1.4:46432017-46432036 None:intergenic 30.0%
TGAGCAAGATTATCACAAAA+AGG - chr1.4:46431865-46431884 None:intergenic 30.0%
TGTAGTTTATGGGTATTTAG+AGG + chr1.4:46432042-46432061 MS.gene034680:CDS 30.0%
TTCAAACGACCAATATCATA+TGG - chr1.4:46432018-46432037 None:intergenic 30.0%
TTCATGTAGAGGGTTTATAT+TGG + chr1.4:46431585-46431604 MS.gene034680:CDS 30.0%
! ACTTTTCGTACAACATACAA+GGG - chr1.4:46432193-46432212 None:intergenic 30.0%
! AGTATACCTTTTTATGGCTT+TGG + chr1.4:46431697-46431716 MS.gene034680:CDS 30.0%
! CTTTTCCAATCATGTGTTTA+TGG + chr1.4:46431614-46431633 MS.gene034680:CDS 30.0%
! GTATACCTTTTTATGGCTTT+GGG + chr1.4:46431698-46431717 MS.gene034680:CDS 30.0%
! TGTTGTTGCCTTTGATTTAA+TGG + chr1.4:46431966-46431985 MS.gene034680:CDS 30.0%
!! ACGAGGATAAAGTTGATATA+TGG + chr1.4:46432091-46432110 MS.gene034680:CDS 30.0%
!! CGAGGATAAAGTTGATATAT+GGG + chr1.4:46432092-46432111 MS.gene034680:CDS 30.0%
AAAGTTAGAGGTTCATGTAG+AGG + chr1.4:46431574-46431593 MS.gene034680:CDS 35.0%
AAATTAGGGTTCAGTAGAAC+AGG - chr1.4:46431526-46431545 None:intergenic 35.0%
AAGTTAGAGGTTCATGTAGA+GGG + chr1.4:46431575-46431594 MS.gene034680:CDS 35.0%
AATCAAAGGCAACAACAAGA+TGG - chr1.4:46431963-46431982 None:intergenic 35.0%
AATGTGTGGAACGAAATTGT+GGG + chr1.4:46431826-46431845 MS.gene034680:CDS 35.0%
ACGAAAAGTGGTGATATTGT+TGG + chr1.4:46432204-46432223 MS.gene034680:CDS 35.0%
AGGTATTTGTCTATGAACTC+CGG - chr1.4:46431650-46431669 None:intergenic 35.0%
ATGACAAAGGAGAGTTTCTA+GGG + chr1.4:46432259-46432278 MS.gene034680:CDS 35.0%
ATGATGTGCTATCTCACATT+GGG + chr1.4:46431785-46431804 MS.gene034680:CDS 35.0%
ATGTAGAGGGTTTATATTGG+TGG + chr1.4:46431588-46431607 MS.gene034680:CDS 35.0%
ATTCCTCTCCCATATGATAT+TGG + chr1.4:46432006-46432025 MS.gene034680:CDS 35.0%
ATTTCTCAGTCTATGGGTTT+GGG + chr1.4:46432065-46432084 MS.gene034680:CDS 35.0%
CTTGTATGTTGTACGAAAAG+TGG + chr1.4:46432192-46432211 MS.gene034680:CDS 35.0%
GGATTCCATAAACACATGAT+TGG - chr1.4:46431622-46431641 None:intergenic 35.0%
TAATGTGTGGAACGAAATTG+TGG + chr1.4:46431825-46431844 MS.gene034680:CDS 35.0%
TACAAGGGAAATGTGAGAAA+TGG - chr1.4:46432178-46432197 None:intergenic 35.0%
TAGAGGATTTCTCAGTCTAT+GGG + chr1.4:46432059-46432078 MS.gene034680:CDS 35.0%
TCACAAAAAGGCAAATGTGT+AGG - chr1.4:46431853-46431872 None:intergenic 35.0%
TCATACCCAAAGCCATAAAA+AGG - chr1.4:46431706-46431725 None:intergenic 35.0%
TTAGAGGATTTCTCAGTCTA+TGG + chr1.4:46432058-46432077 MS.gene034680:CDS 35.0%
TTGGAACAAATAGACCTAGT+GGG + chr1.4:46432223-46432242 MS.gene034680:CDS 35.0%
TTGGTGAGATATGATGACAA+AGG + chr1.4:46432246-46432265 MS.gene034680:CDS 35.0%
! CACTTTTCGTACAACATACA+AGG - chr1.4:46432194-46432213 None:intergenic 35.0%
CGAGTCAACAGATGATTACT+TGG + chr1.4:46431726-46431745 MS.gene034680:CDS 40.0%
CTTGAATGATTAGGCCAAGA+CGG - chr1.4:46431679-46431698 None:intergenic 40.0%
GATGACAAAGGAGAGTTTCT+AGG + chr1.4:46432258-46432277 MS.gene034680:CDS 40.0%
GATGATGTGCTATCTCACAT+TGG + chr1.4:46431784-46431803 MS.gene034680:CDS 40.0%
GATTTCTCAGTCTATGGGTT+TGG + chr1.4:46432064-46432083 MS.gene034680:CDS 40.0%
GTCAACAGATGATTACTTGG+TGG + chr1.4:46431729-46431748 MS.gene034680:CDS 40.0%
GTTGGAACAAATAGACCTAG+TGG + chr1.4:46432222-46432241 MS.gene034680:CDS 40.0%
TATTTGTCTATGAACTCCGG+TGG - chr1.4:46431647-46431666 None:intergenic 40.0%
TCTCTGTATACATAGCCAGT+CGG - chr1.4:46432320-46432339 None:intergenic 40.0%
TGCTCACTTTGCTTATACTG+TGG + chr1.4:46431879-46431898 MS.gene034680:CDS 40.0%
TGCTTATACTGTGGTAGAGT+CGG + chr1.4:46431888-46431907 MS.gene034680:CDS 40.0%
TGTTTATGGAATCCATCCAC+CGG + chr1.4:46431628-46431647 MS.gene034680:CDS 40.0%
TTGGCTATTTGTCCTGTTAC+AGG + chr1.4:46431934-46431953 MS.gene034680:CDS 40.0%
TTTCTCAGTCTATGGGTTTG+GGG + chr1.4:46432066-46432085 MS.gene034680:CDS 40.0%
! AACAAATAGACCTAGTGGGT+TGG + chr1.4:46432227-46432246 MS.gene034680:CDS 40.0%
! TAAGCTTGCTCATTGTCACT+GGG - chr1.4:46432357-46432376 None:intergenic 40.0%
! TCTCTTTGAGAGCTAATGTG+TGG + chr1.4:46431812-46431831 MS.gene034680:CDS 40.0%
! TTAAGCTTGCTCATTGTCAC+TGG - chr1.4:46432358-46432377 None:intergenic 40.0%
ACAAGATGGTCACCTGTAAC+AGG - chr1.4:46431949-46431968 None:intergenic 45.0%
CGACCAATATCATATGGGAG+AGG - chr1.4:46432012-46432031 None:intergenic 45.0%
TAGAACAGGAGCAGAATCAG+CGG - chr1.4:46431512-46431531 None:intergenic 45.0%
TCATATCTCACCAACCCACT+AGG - chr1.4:46432240-46432259 None:intergenic 45.0%
TCTTCAATGGGGCTATTCAC+TGG + chr1.4:46431911-46431930 MS.gene034680:CDS 45.0%
TGGTAGAGTCGGTCTTCAAT+GGG + chr1.4:46431899-46431918 MS.gene034680:CDS 45.0%
TTCACAACATGCATTCCGAC+TGG + chr1.4:46432302-46432321 MS.gene034680:CDS 45.0%
TTCTCAGTCTATGGGTTTGG+GGG + chr1.4:46432067-46432086 MS.gene034680:CDS 45.0%
! AAGCTTGCTCATTGTCACTG+GGG - chr1.4:46432356-46432375 None:intergenic 45.0%
AAATACCTTCGCCTCCGTCT+TGG + chr1.4:46431662-46431681 MS.gene034680:CDS 50.0%
CAATGGGGCTATTCACTGGT+TGG + chr1.4:46431915-46431934 MS.gene034680:CDS 50.0%
GAATGATTAGGCCAAGACGG+AGG - chr1.4:46431676-46431695 None:intergenic 50.0%
GGTAGAGTCGGTCTTCAATG+GGG + chr1.4:46431900-46431919 MS.gene034680:CDS 50.0%
GTGGTAGAGTCGGTCTTCAA+TGG + chr1.4:46431898-46431917 MS.gene034680:CDS 50.0%
TCTCAGTCTATGGGTTTGGG+GGG + chr1.4:46432068-46432087 MS.gene034680:CDS 50.0%
TGTCTATGAACTCCGGTGGA+TGG - chr1.4:46431643-46431662 None:intergenic 50.0%
TCTATGGGTTTGGGGGGACG+AGG + chr1.4:46432074-46432093 MS.gene034680:CDS 60.0%
TTAGGCCAAGACGGAGGCGA+AGG - chr1.4:46431670-46431689 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 46431382 46432377 46431382 ID=MS.gene034680
chr1.4 mRNA 46431382 46432377 46431382 ID=MS.gene034680.t1;Parent=MS.gene034680
chr1.4 exon 46431382 46432377 46431382 ID=MS.gene034680.t1.exon1;Parent=MS.gene034680.t1
chr1.4 CDS 46431382 46432377 46431382 ID=cds.MS.gene034680.t1;Parent=MS.gene034680.t1
Gene Sequence

>MS.gene034680

ATGTGTTTTGCAAATTCACAGTTTCAGATCACTGCTGCAACACACACTCGTCGAATTCTGTTCGTAACAGAACTGTTTGTAACAGAAGCTCCTCAATTTCGATCTATAGCTTTAGATTCATTATTCACCGCTGATTCTGCTCCTGTTCTACTGAACCCTAATTTTTCGCTTCCTAAATCTGAGTTTCATCTTAAAGTTAGAGGTTCATGTAGAGGGTTTATATTGGTGGAATCTTTTCCAATCATGTGTTTATGGAATCCATCCACCGGAGTTCATAGACAAATACCTTCGCCTCCGTCTTGGCCTAATCATTCAAGTATACCTTTTTATGGCTTTGGGTATGACGAGTCAACAGATGATTACTTGGTGGTTTTAGTATTCTATCATTTTATTCAAAACTCAGATGATGTGCTATCTCACATTGGGATTTTCTCTTTGAGAGCTAATGTGTGGAACGAAATTGTGGGTCCTACACATTTGCCTTTTTGTGATAATCTTGCTCACTTTGCTTATACTGTGGTAGAGTCGGTCTTCAATGGGGCTATTCACTGGTTGGCTATTTGTCCTGTTACAGGTGACCATCTTGTTGTTGCCTTTGATTTAATGGAAAGAAAGCTTGTAGAGATTCCTCTCCCATATGATATTGGTCGTTTGAAAATTTGTAGTTTATGGGTATTTAGAGGATTTCTCAGTCTATGGGTTTGGGGGGACGAGGATAAAGTTGATATATGGGTGATGAAAGAATACAAAGTGAAGTCATCTTGGACTAAAACTCTTGTTCTTGATTTGGATACCATTTCTCACATTTCCCTTGTATGTTGTACGAAAAGTGGTGATATTGTTGGAACAAATAGACCTAGTGGGTTGGTGAGATATGATGACAAAGGAGAGTTTCTAGGGCATAACTATTATAGTGAATCTTCACAACATGCATTCCGACTGGCTATGTATACAGAGACTCTGCTTTCACTCCCCAGTGACAATGAGCAAGCTTAA
Protein sequence

>MS.gene034680.t1

MCFANSQFQITAATHTRRILFVTELFVTEAPQFRSIALDSLFTADSAPVLLNPNFSLPKSEFHLKVRGSCRGFILVESFPIMCLWNPSTGVHRQIPSPPSWPNHSSIPFYGFGYDESTDDYLVVLVFYHFIQNSDDVLSHIGIFSLRANVWNEIVGPTHLPFCDNLAHFAYTVVESVFNGAIHWLAICPVTGDHLVVAFDLMERKLVEIPLPYDIGRLKICSLWVFRGFLSLWVWGDEDKVDIWVMKEYKVKSSWTKTLVLDLDTISHISLVCCTKSGDIVGTNRPSGLVRYDDKGEFLGHNYYSESSQHAFRLAMYTETLLSLPSDNEQA