AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1265310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1265310 MtrunA17_Chr4g0072591 81.522 184 6 1 1 156 1 184 1.25e-103 294
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1265310 AT4G25830.1 33.889 180 82 3 4 155 3 173 8.76e-29 104
Msa1265310 AT4G16442.1 31.818 176 95 4 1 152 4 178 7.55e-15 68.6

Find 32 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCAATTGGAGATCATTTA+TGG 0.186265 8_2:-69520393 None:intergenic
TAGTGTGCTGTCACCCATTT+TGG 0.253259 8_2:+69520342 Msa1265310:five_prime_UTR
CATATACAGTGCCTCCAAAA+TGG 0.268682 8_2:-69520356 None:intergenic
TTCAAACGCAAGAACCTCTT+TGG 0.298843 8_2:-69521557 None:intergenic
ATAATAATGCCCCTGCAATT+TGG 0.318489 8_2:-69521460 None:intergenic
TGATATCATAGCCATTAAAA+TGG 0.349665 8_2:-69521498 None:intergenic
TGCAATTTGGTAGCAGAATC+TGG 0.357591 8_2:-69521447 None:intergenic
CCATTGAAATTCCGTATTGT+AGG 0.365530 8_2:-69520791 None:intergenic
TATGTGACATCTGCAGCTGC+TGG 0.381099 8_2:+69520725 Msa1265310:CDS
AGATTCTGCTACCAAATTGC+AGG 0.391434 8_2:+69521449 Msa1265310:CDS
CATTTATGGAATGTAAGATA+TGG 0.418755 8_2:-69520379 None:intergenic
AAAGAGGTTCTTGCGTTTGA+AGG 0.419436 8_2:+69521559 Msa1265310:CDS
TTCTAAATTTCAGTGCCAAT+TGG 0.422565 8_2:-69520406 None:intergenic
CCTACAATACGGAATTTCAA+TGG 0.448706 8_2:+69520791 Msa1265310:CDS
CTTATTATATGCACATGGCT+TGG 0.454545 8_2:+69520816 Msa1265310:CDS
GATTAGTTTCTTATTAGATC+AGG 0.461935 8_2:+69520838 Msa1265310:CDS
TTCCATAAATGATCTCCAAT+TGG 0.472474 8_2:+69520391 Msa1265310:five_prime_UTR
TCTCAGCTTGTCCTACAATA+CGG 0.494365 8_2:+69520780 Msa1265310:CDS
AAACTTCTCCTCTAGCCATT+CGG 0.500585 8_2:-69520470 None:intergenic
ATATACAGTGCCTCCAAAAT+GGG 0.500643 8_2:-69520355 None:intergenic
GAAAGCTACTTACAAGGACT+TGG 0.522093 8_2:+69520587 Msa1265310:CDS
AAGAGGTTCTTGCGTTTGAA+GGG 0.528514 8_2:+69521560 Msa1265310:CDS
AATGGGTGACAGCACACTAC+TGG 0.532835 8_2:-69520338 None:intergenic
TAAGAATGTACTCTCCAAAG+AGG 0.537196 8_2:+69521543 Msa1265310:CDS
ATGTGACATCTGCAGCTGCT+GGG 0.542822 8_2:+69520726 Msa1265310:CDS
GATTCTGCTACCAAATTGCA+GGG 0.554481 8_2:+69521450 Msa1265310:CDS
AGAAGCATTCCAGTGGATGA+AGG 0.566952 8_2:+69521409 Msa1265310:intron
TAAGAAGAAAGCTACTTACA+AGG 0.592857 8_2:+69520581 Msa1265310:CDS
TGGTTCTTATTATATGCACA+TGG 0.599744 8_2:+69520811 Msa1265310:CDS
TGAAGAAATGGAATTCCGAA+TGG 0.602583 8_2:+69520455 Msa1265310:exon
ATGGAATTCCGAATGGCTAG+AGG 0.629083 8_2:+69520462 Msa1265310:CDS
ATTCTGCTACCAAATTGCAG+GGG 0.739306 8_2:+69521451 Msa1265310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATTTTTGTATGTTTTTTT+AGG + chr8_2:69520693-69520712 Msa1265310:intron 15.0%
!! AAAAATTAAAATTGAATGAC+AGG + chr8_2:69521072-69521091 Msa1265310:intron 15.0%
!! AAAATTAAAATTGAATGACA+GGG + chr8_2:69521073-69521092 Msa1265310:intron 15.0%
!!! GAATTTTTGTATGTTTTTTT+AGG + chr8_2:69520693-69520712 Msa1265310:intron 15.0%
!! AAAAATTAAAATTGAATGAC+AGG + chr8_2:69521072-69521091 Msa1265310:intron 15.0%
!! AAAATTAAAATTGAATGACA+GGG + chr8_2:69521073-69521092 Msa1265310:intron 15.0%
!! TACTTCTACATGATAATATA+AGG + chr8_2:69520999-69521018 Msa1265310:intron 20.0%
!!! TTTTTTATGTCAAGAATATG+AGG - chr8_2:69521057-69521076 None:intergenic 20.0%
!!! AAAAAGACAATCATTTTGTT+TGG + chr8_2:69521109-69521128 Msa1265310:intron 20.0%
!! TACTTCTACATGATAATATA+AGG + chr8_2:69520999-69521018 Msa1265310:intron 20.0%
!!! TTTTTTATGTCAAGAATATG+AGG - chr8_2:69521057-69521076 None:intergenic 20.0%
!!! AAAAAGACAATCATTTTGTT+TGG + chr8_2:69521109-69521128 Msa1265310:intron 20.0%
! CATTTATGGAATGTAAGATA+TGG - chr8_2:69520382-69520401 None:intergenic 25.0%
!!! AATTTTCATCTCTGAAGAAA+TGG + chr8_2:69520443-69520462 Msa1265310:exon 25.0%
! AGAGAACAAACAAATAAAGA+GGG - chr8_2:69520633-69520652 None:intergenic 25.0%
! ATATACCATGATCTTGAATT+AGG + chr8_2:69520671-69520690 Msa1265310:intron 25.0%
! AAATTCCTAATTCAAGATCA+TGG - chr8_2:69520679-69520698 None:intergenic 25.0%
!!! GATTAGTTTCTTATTAGATC+AGG + chr8_2:69520838-69520857 Msa1265310:CDS 25.0%
! TACTCGAATCAAAATAAACT+TGG + chr8_2:69520973-69520992 Msa1265310:intron 25.0%
! TCATTTCCTCTTCAAAAATT+AGG + chr8_2:69521026-69521045 Msa1265310:intron 25.0%
! CCAAATGATTATTTCAATCA+AGG - chr8_2:69521294-69521313 None:intergenic 25.0%
! CCTTGATTGAAATAATCATT+TGG + chr8_2:69521291-69521310 Msa1265310:intron 25.0%
! AATAATCATTTGGCAAATCT+TGG + chr8_2:69521301-69521320 Msa1265310:intron 25.0%
!! CTATATAGCATCCATTTTAA+TGG + chr8_2:69521487-69521506 Msa1265310:CDS 25.0%
! TGATATCATAGCCATTAAAA+TGG - chr8_2:69521501-69521520 None:intergenic 25.0%
! CATTTATGGAATGTAAGATA+TGG - chr8_2:69520382-69520401 None:intergenic 25.0%
!!! AATTTTCATCTCTGAAGAAA+TGG + chr8_2:69520443-69520462 Msa1265310:exon 25.0%
! AGAGAACAAACAAATAAAGA+GGG - chr8_2:69520633-69520652 None:intergenic 25.0%
! ATATACCATGATCTTGAATT+AGG + chr8_2:69520671-69520690 Msa1265310:intron 25.0%
! AAATTCCTAATTCAAGATCA+TGG - chr8_2:69520679-69520698 None:intergenic 25.0%
!!! GATTAGTTTCTTATTAGATC+AGG + chr8_2:69520838-69520857 Msa1265310:CDS 25.0%
! TACTCGAATCAAAATAAACT+TGG + chr8_2:69520973-69520992 Msa1265310:intron 25.0%
! TCATTTCCTCTTCAAAAATT+AGG + chr8_2:69521026-69521045 Msa1265310:intron 25.0%
! CCAAATGATTATTTCAATCA+AGG - chr8_2:69521294-69521313 None:intergenic 25.0%
! CCTTGATTGAAATAATCATT+TGG + chr8_2:69521291-69521310 Msa1265310:intron 25.0%
! AATAATCATTTGGCAAATCT+TGG + chr8_2:69521301-69521320 Msa1265310:intron 25.0%
!! CTATATAGCATCCATTTTAA+TGG + chr8_2:69521487-69521506 Msa1265310:CDS 25.0%
! TGATATCATAGCCATTAAAA+TGG - chr8_2:69521501-69521520 None:intergenic 25.0%
TTCCATAAATGATCTCCAAT+TGG + chr8_2:69520391-69520410 Msa1265310:five_prime_UTR 30.0%
TTCTAAATTTCAGTGCCAAT+TGG - chr8_2:69520409-69520428 None:intergenic 30.0%
! TGCTTTTGATACTGAAACAA+AGG + chr8_2:69520542-69520561 Msa1265310:CDS 30.0%
TAAGAAGAAAGCTACTTACA+AGG + chr8_2:69520581-69520600 Msa1265310:CDS 30.0%
GAGAGAACAAACAAATAAAG+AGG - chr8_2:69520634-69520653 None:intergenic 30.0%
TGGTTCTTATTATATGCACA+TGG + chr8_2:69520811-69520830 Msa1265310:CDS 30.0%
AAGTAAGCTCAAATCCAATT+AGG + chr8_2:69520950-69520969 Msa1265310:intron 30.0%
!! TTTTGATTCGAGTACCTAAT+TGG - chr8_2:69520967-69520986 None:intergenic 30.0%
!! GAGATACCTAATTTTTGAAG+AGG - chr8_2:69521035-69521054 None:intergenic 30.0%
! ATTGTCTTTTTCTTCCTACT+GGG - chr8_2:69521100-69521119 None:intergenic 30.0%
GCATAACTCTTAAAGTAGTT+AGG + chr8_2:69521201-69521220 Msa1265310:intron 30.0%
TTGATACTTAACAGTGCATA+TGG + chr8_2:69521261-69521280 Msa1265310:intron 30.0%
TTCCATAAATGATCTCCAAT+TGG + chr8_2:69520391-69520410 Msa1265310:five_prime_UTR 30.0%
TTCTAAATTTCAGTGCCAAT+TGG - chr8_2:69520409-69520428 None:intergenic 30.0%
! TGCTTTTGATACTGAAACAA+AGG + chr8_2:69520542-69520561 Msa1265310:CDS 30.0%
TAAGAAGAAAGCTACTTACA+AGG + chr8_2:69520581-69520600 Msa1265310:CDS 30.0%
GAGAGAACAAACAAATAAAG+AGG - chr8_2:69520634-69520653 None:intergenic 30.0%
TGGTTCTTATTATATGCACA+TGG + chr8_2:69520811-69520830 Msa1265310:CDS 30.0%
AAGTAAGCTCAAATCCAATT+AGG + chr8_2:69520950-69520969 Msa1265310:intron 30.0%
!! TTTTGATTCGAGTACCTAAT+TGG - chr8_2:69520967-69520986 None:intergenic 30.0%
!! GAGATACCTAATTTTTGAAG+AGG - chr8_2:69521035-69521054 None:intergenic 30.0%
! ATTGTCTTTTTCTTCCTACT+GGG - chr8_2:69521100-69521119 None:intergenic 30.0%
GCATAACTCTTAAAGTAGTT+AGG + chr8_2:69521201-69521220 Msa1265310:intron 30.0%
TTGATACTTAACAGTGCATA+TGG + chr8_2:69521261-69521280 Msa1265310:intron 30.0%
ATATACAGTGCCTCCAAAAT+GGG - chr8_2:69520358-69520377 None:intergenic 35.0%
TGCCAATTGGAGATCATTTA+TGG - chr8_2:69520396-69520415 None:intergenic 35.0%
TGAAGAAATGGAATTCCGAA+TGG + chr8_2:69520455-69520474 Msa1265310:exon 35.0%
CCATTGAAATTCCGTATTGT+AGG - chr8_2:69520794-69520813 None:intergenic 35.0%
CCTACAATACGGAATTTCAA+TGG + chr8_2:69520791-69520810 Msa1265310:CDS 35.0%
CTTATTATATGCACATGGCT+TGG + chr8_2:69520816-69520835 Msa1265310:CDS 35.0%
! GATTGTCTTTTTCTTCCTAC+TGG - chr8_2:69521101-69521120 None:intergenic 35.0%
! TTCAAATGCTGAAGTGTTTG+TGG - chr8_2:69521363-69521382 None:intergenic 35.0%
ATAATAATGCCCCTGCAATT+TGG - chr8_2:69521463-69521482 None:intergenic 35.0%
TAAGAATGTACTCTCCAAAG+AGG + chr8_2:69521543-69521562 Msa1265310:CDS 35.0%
ATATACAGTGCCTCCAAAAT+GGG - chr8_2:69520358-69520377 None:intergenic 35.0%
TGCCAATTGGAGATCATTTA+TGG - chr8_2:69520396-69520415 None:intergenic 35.0%
TGAAGAAATGGAATTCCGAA+TGG + chr8_2:69520455-69520474 Msa1265310:exon 35.0%
CCATTGAAATTCCGTATTGT+AGG - chr8_2:69520794-69520813 None:intergenic 35.0%
CCTACAATACGGAATTTCAA+TGG + chr8_2:69520791-69520810 Msa1265310:CDS 35.0%
CTTATTATATGCACATGGCT+TGG + chr8_2:69520816-69520835 Msa1265310:CDS 35.0%
! GATTGTCTTTTTCTTCCTAC+TGG - chr8_2:69521101-69521120 None:intergenic 35.0%
! TTCAAATGCTGAAGTGTTTG+TGG - chr8_2:69521363-69521382 None:intergenic 35.0%
ATAATAATGCCCCTGCAATT+TGG - chr8_2:69521463-69521482 None:intergenic 35.0%
TAAGAATGTACTCTCCAAAG+AGG + chr8_2:69521543-69521562 Msa1265310:CDS 35.0%
CATATACAGTGCCTCCAAAA+TGG - chr8_2:69520359-69520378 None:intergenic 40.0%
AAACTTCTCCTCTAGCCATT+CGG - chr8_2:69520473-69520492 None:intergenic 40.0%
GAAAGCTACTTACAAGGACT+TGG + chr8_2:69520587-69520606 Msa1265310:CDS 40.0%
TCTCAGCTTGTCCTACAATA+CGG + chr8_2:69520780-69520799 Msa1265310:CDS 40.0%
TCCAGTAAGTGCAAAAAGAC+AGG - chr8_2:69521387-69521406 None:intergenic 40.0%
! TGCAATTTGGTAGCAGAATC+TGG - chr8_2:69521450-69521469 None:intergenic 40.0%
AGATTCTGCTACCAAATTGC+AGG + chr8_2:69521449-69521468 Msa1265310:CDS 40.0%
GATTCTGCTACCAAATTGCA+GGG + chr8_2:69521450-69521469 Msa1265310:CDS 40.0%
ATTCTGCTACCAAATTGCAG+GGG + chr8_2:69521451-69521470 Msa1265310:CDS 40.0%
TTCAAACGCAAGAACCTCTT+TGG - chr8_2:69521560-69521579 None:intergenic 40.0%
AAAGAGGTTCTTGCGTTTGA+AGG + chr8_2:69521559-69521578 Msa1265310:CDS 40.0%
AAGAGGTTCTTGCGTTTGAA+GGG + chr8_2:69521560-69521579 Msa1265310:CDS 40.0%
CATATACAGTGCCTCCAAAA+TGG - chr8_2:69520359-69520378 None:intergenic 40.0%
AAACTTCTCCTCTAGCCATT+CGG - chr8_2:69520473-69520492 None:intergenic 40.0%
GAAAGCTACTTACAAGGACT+TGG + chr8_2:69520587-69520606 Msa1265310:CDS 40.0%
TCTCAGCTTGTCCTACAATA+CGG + chr8_2:69520780-69520799 Msa1265310:CDS 40.0%
TCCAGTAAGTGCAAAAAGAC+AGG - chr8_2:69521387-69521406 None:intergenic 40.0%
! TGCAATTTGGTAGCAGAATC+TGG - chr8_2:69521450-69521469 None:intergenic 40.0%
AGATTCTGCTACCAAATTGC+AGG + chr8_2:69521449-69521468 Msa1265310:CDS 40.0%
GATTCTGCTACCAAATTGCA+GGG + chr8_2:69521450-69521469 Msa1265310:CDS 40.0%
ATTCTGCTACCAAATTGCAG+GGG + chr8_2:69521451-69521470 Msa1265310:CDS 40.0%
TTCAAACGCAAGAACCTCTT+TGG - chr8_2:69521560-69521579 None:intergenic 40.0%
AAAGAGGTTCTTGCGTTTGA+AGG + chr8_2:69521559-69521578 Msa1265310:CDS 40.0%
AAGAGGTTCTTGCGTTTGAA+GGG + chr8_2:69521560-69521579 Msa1265310:CDS 40.0%
! TAGTGTGCTGTCACCCATTT+TGG + chr8_2:69520342-69520361 Msa1265310:five_prime_UTR 45.0%
ATGGAATTCCGAATGGCTAG+AGG + chr8_2:69520462-69520481 Msa1265310:CDS 45.0%
! GCCTGTCTTTTTGCACTTAC+TGG + chr8_2:69521383-69521402 Msa1265310:intron 45.0%
!! AGAAGCATTCCAGTGGATGA+AGG + chr8_2:69521409-69521428 Msa1265310:intron 45.0%
TGTTGCATACCTTCATCCAC+TGG - chr8_2:69521421-69521440 None:intergenic 45.0%
! TAGTGTGCTGTCACCCATTT+TGG + chr8_2:69520342-69520361 Msa1265310:five_prime_UTR 45.0%
ATGGAATTCCGAATGGCTAG+AGG + chr8_2:69520462-69520481 Msa1265310:CDS 45.0%
! GCCTGTCTTTTTGCACTTAC+TGG + chr8_2:69521383-69521402 Msa1265310:intron 45.0%
!! AGAAGCATTCCAGTGGATGA+AGG + chr8_2:69521409-69521428 Msa1265310:intron 45.0%
TGTTGCATACCTTCATCCAC+TGG - chr8_2:69521421-69521440 None:intergenic 45.0%
!! ATATAATTATATACAATTAT+AGG + chr8_2:69520902-69520921 Msa1265310:intron 5.0%
!! ATATAATTATATACAATTAT+AGG + chr8_2:69520902-69520921 Msa1265310:intron 5.0%
! TGTGCTGTCACCCATTTTGG+AGG + chr8_2:69520345-69520364 Msa1265310:five_prime_UTR 50.0%
TATGTGACATCTGCAGCTGC+TGG + chr8_2:69520725-69520744 Msa1265310:CDS 50.0%
ATGTGACATCTGCAGCTGCT+GGG + chr8_2:69520726-69520745 Msa1265310:CDS 50.0%
TTGAATGACAGGGACCCAGT+AGG + chr8_2:69521083-69521102 Msa1265310:intron 50.0%
!! CTGGATCAGAAGCATTCCAG+TGG + chr8_2:69521402-69521421 Msa1265310:intron 50.0%
! TGTGCTGTCACCCATTTTGG+AGG + chr8_2:69520345-69520364 Msa1265310:five_prime_UTR 50.0%
TATGTGACATCTGCAGCTGC+TGG + chr8_2:69520725-69520744 Msa1265310:CDS 50.0%
ATGTGACATCTGCAGCTGCT+GGG + chr8_2:69520726-69520745 Msa1265310:CDS 50.0%
TTGAATGACAGGGACCCAGT+AGG + chr8_2:69521083-69521102 Msa1265310:intron 50.0%
!! CTGGATCAGAAGCATTCCAG+TGG + chr8_2:69521402-69521421 Msa1265310:intron 50.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 69520339 69521589 69520339 ID=Msa1265310;Name=Msa1265310
chr8_2 mRNA 69520339 69521589 69520339 ID=Msa1265310-mRNA-1;Parent=Msa1265310;Name=Msa1265310-mRNA-1;_AED=0.30;_eAED=0.30;_QI=123|0.5|0.66|1|0.5|0.66|3|0|156
chr8_2 exon 69520339 69520619 69520339 ID=Msa1265310-mRNA-1:exon:22998;Parent=Msa1265310-mRNA-1
chr8_2 exon 69520715 69520859 69520715 ID=Msa1265310-mRNA-1:exon:22999;Parent=Msa1265310-mRNA-1
chr8_2 exon 69521422 69521589 69521422 ID=Msa1265310-mRNA-1:exon:23000;Parent=Msa1265310-mRNA-1
chr8_2 five_prime_UTR 69520339 69520461 69520339 ID=Msa1265310-mRNA-1:five_prime_utr;Parent=Msa1265310-mRNA-1
chr8_2 CDS 69520462 69520619 69520462 ID=Msa1265310-mRNA-1:cds;Parent=Msa1265310-mRNA-1
chr8_2 CDS 69520715 69520859 69520715 ID=Msa1265310-mRNA-1:cds;Parent=Msa1265310-mRNA-1
chr8_2 CDS 69521422 69521589 69521422 ID=Msa1265310-mRNA-1:cds;Parent=Msa1265310-mRNA-1
Gene Sequence

>Msa1265310

ATGGAATTCCGAATGGCTAGAGGAGAAGTTTTGCTAAGAGTTTCAGCAATATTGTTCTTAGTATCAACAGCTTGTATAGTTGCTTTTGATACTGAAACAAAGGTTGTAATATTAACGATTAAGAAGAAAGCTACTTACAAGGACTTGGATGCTCTCAAGATCTTGTTGTATGTGACATCTGCAGCTGCTGGGTATAACATGCTTCAATTATGCAAACACTCTTTCTCAGCTTGTCCTACAATACGGAATTTCAATGGTTCTTATTATATGCACATGGCTTGGATTAGTTTCTTATTAGATCAGTGGATGAAGGTATGCAACAAATTCACCAGATTCTGCTACCAAATTGCAGGGGCATTATTATGCTGCTATATAGCATCCATTTTAATGGCTATGATATCAACTATCTCAGCTTACAAAGTTTTAAGAATGTACTCTCCAAAGAGGTTCTTGCGTTTGAAGGGAAAATGA

Protein sequence

>Msa1265310

MEFRMARGEVLLRVSAILFLVSTACIVAFDTETKVVILTIKKKATYKDLDALKILLYVTSAAAGYNMLQLCKHSFSACPTIRNFNGSYYMHMAWISFLLDQWMKVCNKFTRFCYQIAGALLCCYIASILMAMISTISAYKVLRMYSPKRFLRLKGK*