AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1298060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1298060 MtrunA17_Chr4g0055431 84.404 327 19 2 1 297 1 325 0.0 546
Msa1298060 MtrunA17_Chr4g0055451 76.968 343 42 3 1 312 1 337 0.0 515
Msa1298060 MtrunA17_Chr4g0045371 62.342 316 87 2 23 308 35 348 1.38e-136 391
Msa1298060 MtrunA17_Chr5g0399911 58.101 358 93 7 2 312 3 350 3.06e-134 385
Msa1298060 MtrunA17_Chr1g0162301 61.386 303 86 2 23 295 36 337 1.21e-129 374
Msa1298060 MtrunA17_Chr8g0381821 62.046 303 82 3 23 295 16 315 1.67e-128 370
Msa1298060 MtrunA17_Chr8g0374571 58.654 312 95 2 23 304 35 342 2.83e-128 370
Msa1298060 MtrunA17_Chr2g0277501 60.577 312 84 4 23 301 16 321 8.61e-128 368
Msa1298060 MtrunA17_Chr8g0385281 62.630 289 73 3 23 278 17 303 8.30e-125 361
Msa1298060 MtrunA17_Chr5g0426521 56.918 318 100 4 25 312 18 328 2.60e-124 359
Msa1298060 MtrunA17_Chr5g0402661 60.345 290 85 1 23 282 30 319 5.54e-124 358
Msa1298060 MtrunA17_Chr3g0131061 61.246 289 77 3 23 278 16 302 4.70e-123 356
Msa1298060 MtrunA17_Chr3g0123791 57.595 316 95 3 1 283 1 310 3.37e-121 351
Msa1298060 MtrunA17_Chr8g0380661 50.177 283 107 3 25 276 18 297 2.00e-95 285
Msa1298060 MtrunA17_Chr2g0302431 32.707 266 128 7 13 237 20 275 2.33e-38 141
Msa1298060 MtrunA17_Chr6g0468751 37.402 254 117 5 23 242 31 276 2.97e-38 141
Msa1298060 MtrunA17_Chr6g0453681 37.008 254 118 5 23 242 31 276 1.57e-37 139
Msa1298060 MtrunA17_Chr4g0063881 34.241 257 118 7 23 240 23 267 1.32e-36 136
Msa1298060 MtrunA17_Chr5g0441011 32.319 263 125 7 26 247 28 278 6.10e-36 134
Msa1298060 MtrunA17_Chr6g0453671 34.766 256 123 7 23 243 31 277 3.89e-34 128
Msa1298060 MtrunA17_Chr1g0150321 34.646 254 114 7 28 241 30 271 4.21e-34 129
Msa1298060 MtrunA17_Chr3g0114511 34.553 246 123 5 28 241 49 288 1.40e-33 128
Msa1298060 MtrunA17_Chr2g0284011 32.400 250 121 6 28 239 29 268 2.83e-33 127
Msa1298060 MtrunA17_Chr5g0432491 31.955 266 126 7 28 257 40 286 3.18e-33 127
Msa1298060 MtrunA17_Chr3g0132671 34.894 235 114 5 39 242 80 306 3.75e-33 127
Msa1298060 MtrunA17_Chr5g0428031 31.923 260 135 6 25 249 23 275 1.41e-32 125
Msa1298060 MtrunA17_Chr3g0107051 33.083 266 126 7 28 253 38 291 2.39e-32 124
Msa1298060 MtrunA17_Chr2g0304111 31.655 278 135 8 13 247 23 288 7.12e-32 123
Msa1298060 MtrunA17_Chr4g0013131 31.855 248 121 7 37 246 38 275 2.10e-31 122
Msa1298060 MtrunA17_Chr8g0346081 33.333 249 123 7 26 239 32 272 3.55e-31 121
Msa1298060 MtrunA17_Chr4g0069301 31.148 244 122 6 48 255 67 300 7.48e-31 120
Msa1298060 MtrunA17_Chr7g0244521 32.941 255 127 7 28 246 29 275 9.99e-31 120
Msa1298060 MtrunA17_Chr8g0379091 33.202 253 114 8 27 237 42 281 1.03e-30 120
Msa1298060 MtrunA17_Chr4g0001331 34.538 249 113 8 28 237 37 274 2.50e-30 119
Msa1298060 MtrunA17_Chr8g0346071 32.800 250 125 7 25 239 31 272 8.71e-30 117
Msa1298060 MtrunA17_Chr5g0432471 33.469 245 111 7 33 237 34 266 1.21e-29 117
Msa1298060 MtrunA17_Chr2g0333821 30.738 244 127 5 27 237 34 268 3.24e-29 115
Msa1298060 MtrunA17_Chr1g0205421 32.046 259 132 7 28 250 29 279 2.12e-28 114
Msa1298060 MtrunA17_Chr3g0107011 32.941 255 127 7 23 241 26 272 2.70e-28 113
Msa1298060 MtrunA17_Chr2g0284031 30.800 250 133 5 28 243 48 291 9.81e-28 112
Msa1298060 MtrunA17_Chr1g0166101 32.143 252 118 8 34 244 47 286 3.64e-27 110
Msa1298060 MtrunA17_Chr7g0244561 32.000 250 120 7 28 237 33 272 2.37e-26 107
Msa1298060 MtrunA17_Chr1g0188031 30.242 248 123 8 37 244 47 284 3.12e-26 107
Msa1298060 MtrunA17_Chr4g0074111 33.684 190 84 4 28 179 35 220 1.31e-24 102
Msa1298060 MtrunA17_Chr8g0351491 28.571 252 137 6 23 240 28 270 1.78e-23 97.4
Msa1298060 MtrunA17_Chr3g0140631 26.190 252 143 6 23 240 49 291 3.13e-22 94.4
Msa1298060 MtrunA17_Chr8g0353621 27.778 252 139 6 23 240 22 264 4.22e-22 93.6
Msa1298060 MtrunA17_Chr4g0008561 28.279 244 138 6 28 237 36 276 1.75e-20 92.0
Msa1298060 MtrunA17_Chr3g0091281 33.862 189 80 5 28 177 29 211 4.18e-19 84.7
Msa1298060 MtrunA17_Chr3g0125381 29.858 211 110 6 61 240 6 209 6.50e-19 83.6
Msa1298060 MtrunA17_Chr5g0444171 30.315 254 131 8 23 237 104 350 1.96e-18 85.5
Msa1298060 MtrunA17_Chr8g0345801 27.848 237 123 8 28 224 32 260 2.09e-18 83.2
Msa1298060 MtrunA17_Chr4g0029241 26.800 250 135 9 52 263 168 407 3.75e-18 84.3
Msa1298060 MtrunA17_Chr2g0326531 28.452 239 127 8 52 254 170 400 1.19e-17 82.8
Msa1298060 MtrunA17_Chr1g0176751 31.579 266 138 9 12 238 123 383 1.96e-17 82.8
Msa1298060 MtrunA17_Chr4g0072831 28.700 223 126 8 25 237 24 223 1.85e-16 78.6
Msa1298060 MtrunA17_Chr7g0274941 34.507 142 84 4 101 239 141 276 1.87e-16 78.2
Msa1298060 MtrunA17_Chr3g0129721 27.234 235 135 6 28 228 42 274 1.84e-14 72.4
Msa1298060 MtrunA17_Chr8g0355131 30.275 218 113 6 52 236 94 305 2.00e-14 73.6
Msa1298060 MtrunA17_Chr4g0045261 28.000 250 137 8 25 236 154 398 2.35e-14 73.6
Msa1298060 MtrunA17_Chr3g0131181 28.455 246 134 7 32 240 50 290 2.94e-14 73.2
Msa1298060 MtrunA17_Chr1g0190131 27.053 207 113 5 64 239 67 266 3.99e-13 68.6
Msa1298060 MtrunA17_Chr3g0135951 35.156 128 72 5 113 237 282 401 5.41e-13 69.3
Msa1298060 MtrunA17_Chr1g0187581 37.736 106 59 4 135 237 315 416 2.93e-12 67.0
Msa1298060 MtrunA17_Chr1g0167311 30.612 196 120 10 50 237 227 414 5.64e-12 66.2
Msa1298060 MtrunA17_Chr1g0167351 28.972 214 131 9 35 237 207 410 8.24e-12 65.9
Msa1298060 MtrunA17_Chr1g0153321 25.850 294 134 12 23 237 120 408 9.16e-12 65.5
Msa1298060 MtrunA17_Chr4g0059751 27.854 219 112 9 54 237 145 352 1.88e-11 64.3
Msa1298060 MtrunA17_Chr3g0127571 27.519 258 137 10 23 237 150 400 2.64e-11 64.3
Msa1298060 MtrunA17_Chr1g0183651 27.684 177 111 6 50 219 232 398 8.94e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1298060 AT4G33950.1 62.338 308 84 2 23 300 33 338 8.12e-135 387
Msa1298060 AT4G33950.2 61.967 305 86 1 23 297 33 337 1.53e-133 383
Msa1298060 AT3G50500.1 60.738 298 87 1 23 290 35 332 1.14e-128 371
Msa1298060 AT5G08590.1 60.535 299 88 1 23 291 16 314 7.79e-127 366
Msa1298060 AT5G66880.1 60.884 294 85 1 23 286 34 327 9.98e-127 366
Msa1298060 AT5G66880.2 60.884 294 85 1 23 286 34 327 9.98e-127 366
Msa1298060 AT3G50500.2 59.344 305 87 2 23 290 35 339 7.73e-126 364
Msa1298060 AT5G63650.1 62.081 298 78 3 23 287 16 311 8.02e-126 364
Msa1298060 AT1G10940.1 62.630 289 73 3 23 278 16 302 2.82e-125 363
Msa1298060 AT1G60940.1 60.066 303 85 4 23 291 16 316 5.19e-124 359
Msa1298060 AT1G60940.2 60.066 303 85 4 23 291 16 316 5.19e-124 359
Msa1298060 AT1G10940.2 60.943 297 73 3 23 278 16 310 6.09e-124 360
Msa1298060 AT1G78290.3 61.324 287 74 3 23 278 16 296 3.19e-116 339
Msa1298060 AT1G78290.2 61.324 287 74 3 23 278 16 296 3.19e-116 339
Msa1298060 AT4G40010.1 58.804 301 88 3 23 287 16 316 8.24e-114 333
Msa1298060 AT2G23030.1 54.517 321 107 4 23 308 16 332 1.59e-113 332
Msa1298060 AT5G08590.2 56.723 238 73 1 84 291 1 238 8.05e-91 272
Msa1298060 AT5G35410.2 35.798 257 110 7 25 240 25 267 6.06e-39 141
Msa1298060 AT4G24400.2 34.717 265 121 8 25 249 23 275 6.27e-39 142
Msa1298060 AT3G29160.3 37.008 254 118 5 23 242 32 277 9.64e-39 140
Msa1298060 AT3G01090.3 37.008 254 118 5 23 242 31 276 1.08e-38 143
Msa1298060 AT3G01090.1 37.008 254 118 5 23 242 31 276 1.08e-38 143
Msa1298060 AT3G01090.2 37.008 254 118 5 23 242 54 299 1.75e-38 143
Msa1298060 AT4G24400.1 34.717 265 121 8 25 249 23 275 1.78e-38 141
Msa1298060 AT5G35410.1 35.798 257 110 7 25 240 25 267 1.79e-38 141
Msa1298060 AT5G25110.1 34.035 285 132 9 1 241 29 301 2.17e-38 142
Msa1298060 AT3G29160.1 37.008 254 118 5 23 242 32 277 1.15e-37 140
Msa1298060 AT3G29160.2 37.008 254 118 5 23 242 32 277 1.15e-37 140
Msa1298060 AT5G10930.1 35.341 249 108 7 34 241 35 271 4.53e-37 137
Msa1298060 AT5G21326.1 34.601 263 119 7 26 247 28 278 1.33e-36 136
Msa1298060 AT1G48260.1 33.459 266 126 7 13 237 11 266 6.86e-35 131
Msa1298060 AT3G17510.1 32.707 266 128 7 13 237 20 275 5.94e-34 129
Msa1298060 AT2G25090.1 32.296 257 117 6 32 241 36 282 6.56e-34 129
Msa1298060 AT2G26980.1 31.955 266 124 8 25 247 28 279 1.37e-33 127
Msa1298060 AT5G39440.1 35.039 254 118 7 25 242 33 275 3.33e-33 128
Msa1298060 AT2G26980.5 31.955 266 124 8 25 247 28 279 3.95e-33 126
Msa1298060 AT1G30270.1 29.412 306 149 9 13 275 31 312 3.99e-33 127
Msa1298060 AT1G30270.2 29.210 291 141 8 26 275 46 312 4.35e-33 127
Msa1298060 AT2G26980.3 31.716 268 126 8 23 247 26 279 4.68e-33 127
Msa1298060 AT2G26980.4 31.955 266 124 8 25 247 38 289 4.72e-33 127
Msa1298060 AT1G01140.3 32.222 270 128 8 13 239 19 276 8.74e-33 126
Msa1298060 AT1G01140.1 32.222 270 128 8 13 239 19 276 9.11e-33 126
Msa1298060 AT1G01140.2 31.852 270 131 8 13 239 19 278 2.14e-32 125
Msa1298060 AT2G30360.1 31.496 254 120 7 28 239 38 279 2.21e-32 125
Msa1298060 AT5G21222.1 31.379 290 150 6 23 277 25 300 3.74e-32 126
Msa1298060 AT5G21222.2 31.379 290 150 6 23 277 25 300 3.74e-32 126
Msa1298060 AT5G21222.4 31.379 290 150 6 23 277 25 300 3.74e-32 126
Msa1298060 AT5G21222.3 31.379 290 150 6 23 277 25 300 3.74e-32 126
Msa1298060 AT5G45820.1 30.903 288 137 8 28 275 29 294 2.84e-31 122
Msa1298060 AT5G58380.1 32.932 249 115 8 33 241 34 270 4.44e-31 122
Msa1298060 AT2G38490.1 34.866 261 122 7 31 253 49 299 5.64e-31 120
Msa1298060 AT4G14580.1 31.765 255 124 7 27 241 37 281 1.13e-30 120
Msa1298060 AT5G45810.1 31.727 249 130 5 27 241 44 286 1.24e-30 120
Msa1298060 AT4G18700.1 31.325 249 131 5 27 241 42 284 3.54e-30 119
Msa1298060 AT3G23000.1 32.171 258 118 9 27 241 41 284 1.23e-29 117
Msa1298060 AT2G34180.1 32.129 249 129 6 27 241 73 315 1.73e-29 117
Msa1298060 AT5G01810.3 31.890 254 121 7 28 241 29 270 1.87e-29 115
Msa1298060 AT5G01810.1 31.890 254 121 7 28 241 29 270 1.90e-29 116
Msa1298060 AT5G01810.2 31.890 254 121 7 28 241 29 270 1.90e-29 116
Msa1298060 AT2G26980.2 32.365 241 106 8 25 222 28 254 5.81e-29 112
Msa1298060 AT2G45490.1 30.556 252 132 6 23 240 34 276 4.36e-28 110
Msa1298060 AT1G29230.1 30.924 249 132 5 27 241 90 332 5.15e-27 110
Msa1298060 AT4G30960.1 32.018 228 103 7 50 239 67 280 6.30e-27 110
Msa1298060 AT5G07070.1 29.644 253 130 7 27 241 28 270 2.71e-26 108
Msa1298060 AT5G57630.2 30.894 246 128 7 26 237 27 264 1.41e-25 105
Msa1298060 AT5G57630.1 30.894 246 128 7 26 237 27 264 1.99e-25 105
Msa1298060 AT2G25880.2 27.928 222 145 5 23 240 37 247 2.29e-25 102
Msa1298060 AT4G11373.1 66.667 72 24 0 39 110 1 72 3.17e-25 97.8
Msa1298060 AT3G17510.2 32.836 201 90 5 72 237 5 195 4.58e-24 100
Msa1298060 AT1G30270.3 28.452 239 110 7 74 275 1 215 2.48e-23 99.4
Msa1298060 AT1G48260.2 33.508 191 82 5 82 237 1 181 3.16e-23 98.6
Msa1298060 AT1G48260.4 33.508 191 82 5 82 237 1 181 3.16e-23 98.6
Msa1298060 AT1G48260.3 33.508 191 82 5 82 237 1 181 3.16e-23 98.6
Msa1298060 AT1G48260.5 33.508 191 82 5 82 237 1 181 3.16e-23 98.6
Msa1298060 AT4G32830.1 26.190 252 143 6 23 240 43 285 1.16e-22 95.9
Msa1298060 AT5G01820.1 31.452 248 122 7 28 237 39 276 4.14e-22 96.3
Msa1298060 AT2G25880.1 25.000 252 146 6 23 240 37 279 9.77e-22 93.2
Msa1298060 AT3G53930.2 26.909 275 156 8 34 268 43 312 1.05e-21 95.9
Msa1298060 AT3G53930.1 26.909 275 156 8 34 268 43 312 1.10e-21 95.9
Msa1298060 AT2G25880.4 25.000 252 146 6 23 240 50 292 1.51e-21 93.2
Msa1298060 AT2G25880.3 25.000 252 146 6 23 240 98 340 5.99e-21 92.0
Msa1298060 AT3G61960.1 28.409 264 145 10 27 253 26 282 8.53e-21 93.2
Msa1298060 AT3G61960.2 28.409 264 145 9 27 253 26 282 9.70e-21 92.8
Msa1298060 AT2G37840.1 27.347 245 139 7 28 237 29 269 1.46e-19 89.7
Msa1298060 AT5G62310.1 26.710 307 149 10 23 260 766 1065 1.27e-18 87.0
Msa1298060 AT5G23580.1 28.464 267 147 8 13 240 22 283 2.16e-18 85.9
Msa1298060 AT1G12580.1 26.966 267 150 7 23 253 56 313 4.30e-18 85.1
Msa1298060 AT2G38910.1 30.588 255 127 10 23 236 146 391 5.76e-18 84.7
Msa1298060 AT2G31500.1 31.818 242 123 9 32 236 87 323 2.02e-17 83.2
Msa1298060 AT1G48490.3 25.503 298 146 12 23 254 840 1127 2.33e-17 83.2
Msa1298060 AT1G48490.2 25.503 298 146 12 23 254 840 1127 2.33e-17 83.2
Msa1298060 AT1G48490.1 25.503 298 146 12 23 254 840 1127 2.33e-17 83.2
Msa1298060 AT1G48490.4 25.503 298 146 12 23 254 841 1128 2.38e-17 83.2
Msa1298060 AT3G45240.3 28.372 215 116 8 52 236 159 365 4.75e-17 81.3
Msa1298060 AT4G04740.2 29.719 249 131 7 27 237 85 327 8.53e-17 81.3
Msa1298060 AT4G04740.4 29.719 249 131 7 27 237 85 327 8.59e-17 81.3
Msa1298060 AT4G04740.6 29.719 249 131 7 27 237 85 327 8.59e-17 81.3
Msa1298060 AT4G04740.5 29.719 249 131 7 27 237 85 327 8.59e-17 81.3
Msa1298060 AT4G04740.3 29.719 249 131 7 27 237 85 327 8.59e-17 81.3
Msa1298060 AT4G04740.1 29.719 249 131 7 27 237 85 327 8.59e-17 81.3
Msa1298060 AT4G04740.7 29.719 249 131 7 27 237 85 327 8.59e-17 81.3
Msa1298060 AT3G45240.2 28.440 218 115 9 52 236 159 368 2.73e-16 79.3
Msa1298060 AT3G45240.1 28.440 218 115 9 52 236 159 368 2.73e-16 79.3
Msa1298060 AT4G09570.1 30.075 266 140 9 13 238 25 284 4.34e-16 79.0
Msa1298060 AT1G35670.1 29.811 265 142 8 13 238 26 285 5.27e-16 78.6
Msa1298060 AT5G60550.1 26.070 257 129 10 52 257 158 404 8.33e-16 77.8
Msa1298060 AT5G57630.3 31.875 160 96 5 79 237 29 176 1.05e-14 73.9
Msa1298060 AT2G17890.1 29.457 258 128 9 23 236 120 367 2.22e-14 73.9
Msa1298060 AT2G20470.2 27.835 194 126 7 50 237 236 421 8.67e-12 66.2
Msa1298060 AT2G20470.1 27.835 194 126 7 50 237 236 421 8.67e-12 66.2
Msa1298060 AT2G20470.3 27.835 194 126 7 50 237 236 421 1.02e-11 65.9
Msa1298060 AT5G66210.3 26.877 253 141 8 23 236 74 321 2.63e-11 64.7
Msa1298060 AT5G66210.5 26.877 253 141 8 23 236 74 321 3.09e-11 64.3
Msa1298060 AT5G66210.1 26.877 253 141 8 23 236 74 321 3.85e-11 63.9
Msa1298060 AT5G66210.2 26.877 253 141 8 23 236 74 321 3.85e-11 63.9
Msa1298060 AT5G66210.6 26.877 253 141 8 23 236 74 321 4.41e-11 63.9
Msa1298060 AT5G66210.4 26.877 253 141 8 23 236 74 321 4.41e-11 63.9
Msa1298060 AT3G04530.1 27.404 208 114 8 64 239 69 271 7.32e-11 62.4

Find 91 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAATTTCTAGGATCTTC+TGG 0.188809 8_3:-58710940 None:intergenic
GCAGGACCAGGTTCCTTTAA+TGG 0.222418 8_3:-58709124 None:intergenic
ATAGTCTTTCTGAAATTTCT+AGG 0.223677 8_3:-58710949 None:intergenic
TCTTTGAATCTGATAATATT+AGG 0.251887 8_3:-58709475 None:intergenic
TTGTCATGCAGGTCTGAAAT+TGG 0.254905 8_3:+58710412 Msa1298060:intron
CTCGTCATCTTCGATGTTCA+TGG 0.271720 8_3:-58711634 None:intergenic
GAAATTTCAGAAAGACTATT+GGG 0.306280 8_3:+58710953 Msa1298060:CDS
TCTGGTTGGTAGAGAATAAT+AGG 0.320796 8_3:+58711055 Msa1298060:intron
TCAGCTTCAATTATCTCTTC+AGG 0.323000 8_3:-58711477 None:intergenic
AGAAATTTCAGAAAGACTAT+TGG 0.328126 8_3:+58710952 Msa1298060:CDS
GTACATAGAGAGGGGTAAAA+AGG 0.349105 8_3:+58709192 Msa1298060:CDS
CTTCTACACTTTGGCTTGGT+TGG 0.363648 8_3:-58711530 None:intergenic
ACTATGAAAATGTGTGATTC+AGG 0.382583 8_3:+58711676 Msa1298060:exon
GGATCTTCTGGTTCTTCAAA+TGG 0.399417 8_3:-58710928 None:intergenic
ATTTCTTCTACACTTTGGCT+TGG 0.419242 8_3:-58711534 None:intergenic
AAATACAATGCCAACAGAAA+AGG 0.425652 8_3:-58711728 None:intergenic
ATAAAGAGGATCACCATTCC+AGG 0.431331 8_3:+58711420 Msa1298060:intron
ACACTTTATGTTATGCTAGT+TGG 0.432447 8_3:+58710898 Msa1298060:CDS
TAATTAACCACAGATCATTA+AGG 0.432582 8_3:+58709449 Msa1298060:CDS
GAAATTGGAAAACACTCTCT+TGG 0.437175 8_3:+58710427 Msa1298060:CDS
GGAGATCCAACTGTTGCCTT+TGG 0.441401 8_3:-58710614 None:intergenic
ATCTTCGATGTTCATGGAAC+CGG 0.442792 8_3:-58711628 None:intergenic
TTCTCATCTAGTCATTGTCT+TGG 0.446749 8_3:+58710083 Msa1298060:CDS
TCAGGCCTTCATTTGGTGAA+TGG 0.449521 8_3:+58711694 Msa1298060:three_prime_UTR
TCATGATTTCTTCTACACTT+TGG 0.458463 8_3:-58711539 None:intergenic
ATGACGAGGAAATTGATGTC+AGG 0.467505 8_3:+58711649 Msa1298060:CDS
CAACAAAGATGCGAGATAGA+AGG 0.472933 8_3:-58711129 None:intergenic
GGCACATACCTTGGCAGGAT+CGG 0.473924 8_3:-58711152 None:intergenic
ATAATTGAAGCTGAAAGAAA+AGG 0.475849 8_3:+58711486 Msa1298060:CDS
ACATTGGCACATACCTTGGC+AGG 0.476610 8_3:-58711157 None:intergenic
GCTGGCGATGGTGGTCAAGC+TGG 0.479663 8_3:+58711603 Msa1298060:CDS
CAGGGATAAAACAGGACCCT+TGG 0.480753 8_3:+58711439 Msa1298060:CDS
TCCCGAGGTTCTGTTACAAA+AGG 0.485145 8_3:+58710649 Msa1298060:CDS
AATACAATGCCAACAGAAAA+GGG 0.490195 8_3:-58711727 None:intergenic
AATATGTACATCGTCGGGTA+TGG 0.497651 8_3:-58711089 None:intergenic
CACCATTCCAGGGATAAAAC+AGG 0.500844 8_3:+58711431 Msa1298060:CDS
AGAACGTTATGAGCCATTAG+AGG 0.507137 8_3:+58709078 Msa1298060:CDS
ACTGAAATATGTACATCGTC+GGG 0.507583 8_3:-58711094 None:intergenic
TTGTCTTTAGCCAACCTAGC+AGG 0.512029 8_3:-58709142 None:intergenic
TTGGAAAACACTCTCTTGGA+TGG 0.513601 8_3:+58710431 Msa1298060:CDS
TTCAAGAGGATACGCACTGC+TGG 0.528814 8_3:+58710129 Msa1298060:CDS
GGATCAAAAGCTGGCGATGG+TGG 0.530656 8_3:+58711594 Msa1298060:CDS
GTGTGATTCAGGCCTTCATT+TGG 0.532432 8_3:+58711687 Msa1298060:exon
ATTGTCTTGGAGTATGCTGC+TGG 0.535131 8_3:+58710096 Msa1298060:CDS
GGAACCTGGTCCTGCTAGGT+TGG 0.541285 8_3:+58709132 Msa1298060:CDS
ATACAAGAAGCGAGGGTACC+GGG 0.542520 8_3:+58711567 Msa1298060:CDS
AGCTGAAAGAAAAGGTTATG+TGG 0.543791 8_3:+58711494 Msa1298060:CDS
GATGGTGGTCAAGCTGGCAC+CGG 0.547959 8_3:+58711609 Msa1298060:CDS
TTAGCCAACCTAGCAGGACC+AGG 0.548718 8_3:-58709136 None:intergenic
GACATCCATTCACCAAATGA+AGG 0.553740 8_3:-58711699 None:intergenic
GACTGAAATATGTACATCGT+CGG 0.553919 8_3:-58711095 None:intergenic
CTTTGTTGCCGATCCTGCCA+AGG 0.554698 8_3:+58711144 Msa1298060:CDS
AACATAAAGTGTCACACCAC+AGG 0.555054 8_3:-58710887 None:intergenic
CATACAAGAAGCGAGGGTAC+CGG 0.556086 8_3:+58711566 Msa1298060:CDS
TAAAGGAACCTGGTCCTGCT+AGG 0.558021 8_3:+58709128 Msa1298060:CDS
TTTCAACTTTGATTACTCCA+AGG 0.567788 8_3:+58710478 Msa1298060:CDS
ACCTCGGGAGTAATGTATGA+AGG 0.570992 8_3:-58710635 None:intergenic
AGGTTATGTGGAAACAAAGA+AGG 0.571294 8_3:+58711506 Msa1298060:CDS
TCTCAACCAAAGGCAACAGT+TGG 0.573037 8_3:+58710608 Msa1298060:CDS
AATATTGCATTCTCAACCAA+AGG 0.573545 8_3:+58710598 Msa1298060:CDS
TTGTATAGATTGCAGATGTG+TGG 0.578652 8_3:+58710863 Msa1298060:intron
CCGGGACAAGGATCAAAAGC+TGG 0.579489 8_3:+58711585 Msa1298060:CDS
TGACGAGGAAATTGATGTCA+GGG 0.585447 8_3:+58711650 Msa1298060:CDS
AAGACAATGACTAGATGAGA+AGG 0.585565 8_3:-58710081 None:intergenic
TGCTCTCAAGTACATAGAGA+GGG 0.588141 8_3:+58709183 Msa1298060:CDS
GAAGCGAGGGTACCGGGACA+AGG 0.594784 8_3:+58711573 Msa1298060:CDS
AGACAATGACTAGATGAGAA+GGG 0.596168 8_3:-58710080 None:intergenic
AATCAGCCATTAAAGGAACC+TGG 0.597077 8_3:+58709118 Msa1298060:CDS
ATTGCAGATGTGTGGTCCTG+TGG 0.607065 8_3:+58710871 Msa1298060:CDS
CAAGGATCAAAAGCTGGCGA+TGG 0.610976 8_3:+58711591 Msa1298060:CDS
TCCTTCATACATTACTCCCG+AGG 0.611395 8_3:+58710634 Msa1298060:CDS
TACATATTTCAGTCGAGTGT+AGG 0.615992 8_3:+58711104 Msa1298060:CDS
GTCTTGGAGTATGCTGCTGG+TGG 0.616092 8_3:+58710099 Msa1298060:CDS
TAGGATGCCTTAATGATCTG+TGG 0.616104 8_3:-58709456 None:intergenic
GACAATGACTAGATGAGAAG+GGG 0.619538 8_3:-58710079 None:intergenic
TGGATGGAAATCCATCCCCT+CGG 0.620502 8_3:+58710447 Msa1298060:CDS
TAATATTATCAGATTCAAAG+AGG 0.622110 8_3:+58709477 Msa1298060:CDS
ACAAAAGGAGTACGACGGCA+AGG 0.624488 8_3:+58710664 Msa1298060:CDS
GAGGATCATACAAGAAGCGA+GGG 0.631597 8_3:+58711560 Msa1298060:CDS
TTGCTCTCAAGTACATAGAG+AGG 0.636123 8_3:+58709182 Msa1298060:CDS
AGATTGATGAGAGTGTCCAC+AGG 0.637758 8_3:+58709422 Msa1298060:intron
CATGAACATCGAAGATGACG+AGG 0.642337 8_3:+58711635 Msa1298060:CDS
TGAGGATCATACAAGAAGCG+AGG 0.644704 8_3:+58711559 Msa1298060:CDS
TAAAGAGGATCACCATTCCA+GGG 0.653139 8_3:+58711421 Msa1298060:intron
CTGGTGGTGAACTCTTCAAG+AGG 0.659293 8_3:+58710115 Msa1298060:CDS
AAATTTCAGAAAGACTATTG+GGG 0.660159 8_3:+58710954 Msa1298060:CDS
AAAGTGTAGAAGAAATCATG+AGG 0.660784 8_3:+58711541 Msa1298060:CDS
GATTGATGAGAGTGTCCACA+GGG 0.673045 8_3:+58709423 Msa1298060:intron
GCTCTCAAGTACATAGAGAG+GGG 0.692576 8_3:+58709184 Msa1298060:CDS
TGTGGTTAATTATGTCCCTG+TGG 0.711515 8_3:-58709438 None:intergenic
CTGTTACAAAAGGAGTACGA+CGG 0.728092 8_3:+58710659 Msa1298060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATTTCAATATATATTTT+TGG - chr8_3:58711776-58711795 None:intergenic 10.0%
!! AACATTAATTAATGTTACAT+TGG - chr8_3:58711176-58711195 None:intergenic 15.0%
!! ATTTGTAATTCTAATGTTAT+AGG - chr8_3:58711239-58711258 None:intergenic 15.0%
!!! TAAATTATTTTCCATATCTT+AGG - chr8_3:58711276-58711295 None:intergenic 15.0%
!! AAATAAAAAAGTTCCTCTAA+TGG - chr8_3:58709094-58709113 None:intergenic 20.0%
!! TCTTTGAATCTGATAATATT+AGG - chr8_3:58709478-58709497 None:intergenic 20.0%
!! TAATATTATCAGATTCAAAG+AGG + chr8_3:58709477-58709496 Msa1298060:CDS 20.0%
!!! TGTATACGATTTTCATTAAA+AGG - chr8_3:58710213-58710232 None:intergenic 20.0%
!!! AAATTTCCTATATTTGTTAC+AGG + chr8_3:58710259-58710278 Msa1298060:intron 20.0%
!! GATATCTTATATTATATCCA+AGG + chr8_3:58710521-58710540 Msa1298060:intron 20.0%
!! TTAACAAATACTATTAACCT+TGG - chr8_3:58710541-58710560 None:intergenic 20.0%
!! ATTAGAATTACAAATGTTCA+TGG + chr8_3:58711244-58711263 Msa1298060:intron 20.0%
!! AAAAGAATTAATGCAAATAC+AGG + chr8_3:58711335-58711354 Msa1298060:intron 20.0%
!!! TTTTTTGAATCAGCCATTAA+AGG + chr8_3:58709111-58709130 Msa1298060:CDS 25.0%
! AGCAAAAAATCAATTCAATG+AGG - chr8_3:58709236-58709255 None:intergenic 25.0%
! TTACCTTAAACTCTTCATTA+AGG + chr8_3:58709338-58709357 Msa1298060:intron 25.0%
! TATCCTTAATGAAGAGTTTA+AGG - chr8_3:58709344-58709363 None:intergenic 25.0%
! TAATTAACCACAGATCATTA+AGG + chr8_3:58709449-58709468 Msa1298060:CDS 25.0%
!! TCTTAAACTGTTTCTTCTTT+AGG + chr8_3:58709515-58709534 Msa1298060:intron 25.0%
!!! TTTCTTCTTTAGGTTTTGTA+GGG + chr8_3:58709525-58709544 Msa1298060:intron 25.0%
!!! AAAGAGCTTTTGGAAAAATT+GGG - chr8_3:58709858-58709877 None:intergenic 25.0%
! AGAATGATCAAATTTCGATT+CGG - chr8_3:58709903-58709922 None:intergenic 25.0%
! CATCATTACTAAAAGAAAGA+AGG + chr8_3:58709948-58709967 Msa1298060:intron 25.0%
!!! CATTTTGTTATAACTTCTGA+AGG - chr8_3:58710025-58710044 None:intergenic 25.0%
! ATATGAAATACTGTTAACCT+TGG - chr8_3:58710498-58710517 None:intergenic 25.0%
! ATTTGTCAATTGTTAAACCA+TGG - chr8_3:58710762-58710781 None:intergenic 25.0%
!! TGATTAAAAAGTTCACGTTT+TGG + chr8_3:58710803-58710822 Msa1298060:intron 25.0%
!!! ATAGTCTTTCTGAAATTTCT+AGG - chr8_3:58710952-58710971 None:intergenic 25.0%
! AGAAATTTCAGAAAGACTAT+TGG + chr8_3:58710952-58710971 Msa1298060:CDS 25.0%
! GAAATTTCAGAAAGACTATT+GGG + chr8_3:58710953-58710972 Msa1298060:CDS 25.0%
! AAATTTCAGAAAGACTATTG+GGG + chr8_3:58710954-58710973 Msa1298060:CDS 25.0%
! ATAATTTAACTAATCAACCG+TGG + chr8_3:58711288-58711307 Msa1298060:intron 25.0%
!! AATTCTTTTAGTGATGTTAG+AGG - chr8_3:58711324-58711343 None:intergenic 25.0%
!!! GCAAATTTTTCAAAAACCAA+GGG - chr8_3:58711458-58711477 None:intergenic 25.0%
! ATAATTGAAGCTGAAAGAAA+AGG + chr8_3:58711486-58711505 Msa1298060:CDS 25.0%
TTGGCTAAAGACAAAAATAC+TGG + chr8_3:58709151-58709170 Msa1298060:CDS 30.0%
!! GTTTCTTCTTTAGGTTTTGT+AGG + chr8_3:58709524-58709543 Msa1298060:intron 30.0%
!! TTCTTCTTTAGGTTTTGTAG+GGG + chr8_3:58709526-58709545 Msa1298060:intron 30.0%
GGCATTATGAATGTAGTATT+TGG + chr8_3:58709547-58709566 Msa1298060:intron 30.0%
! ACATGATTTTCCTTATGAGT+CGG - chr8_3:58709666-58709685 None:intergenic 30.0%
CAATGAGTTATTATCGTTGA+CGG + chr8_3:58709708-58709727 Msa1298060:intron 30.0%
AATGAGTTATTATCGTTGAC+GGG + chr8_3:58709709-58709728 Msa1298060:intron 30.0%
GCTAATAGCTAAAATACTCA+TGG - chr8_3:58709804-58709823 None:intergenic 30.0%
!! AAGAGCTTTTGGAAAAATTG+GGG - chr8_3:58709857-58709876 None:intergenic 30.0%
!! GAAAGAGCTTTTGGAAAAAT+TGG - chr8_3:58709859-58709878 None:intergenic 30.0%
! AAGCTAAATGAAAGAGCTTT+TGG - chr8_3:58709868-58709887 None:intergenic 30.0%
ATTCTGCGCAAAAAGTTATA+CGG + chr8_3:58709918-58709937 Msa1298060:intron 30.0%
ATCTTGCCTGTAACAAATAT+AGG - chr8_3:58710268-58710287 None:intergenic 30.0%
TTATTCCAACAGCTAATATC+TGG + chr8_3:58710290-58710309 Msa1298060:intron 30.0%
TATAATATGCTTTGTGCTGT+AGG + chr8_3:58710385-58710404 Msa1298060:intron 30.0%
! AGTTGAAAATTTTAAGCCGA+GGG - chr8_3:58710466-58710485 None:intergenic 30.0%
! AAGTTGAAAATTTTAAGCCG+AGG - chr8_3:58710467-58710486 None:intergenic 30.0%
TTTCAACTTTGATTACTCCA+AGG + chr8_3:58710478-58710497 Msa1298060:CDS 30.0%
AATATTGCATTCTCAACCAA+AGG + chr8_3:58710598-58710617 Msa1298060:CDS 30.0%
! GTCAATTGAGTTAGTGTTTT+TGG + chr8_3:58710835-58710854 Msa1298060:intron 30.0%
ACACTTTATGTTATGCTAGT+TGG + chr8_3:58710898-58710917 Msa1298060:CDS 30.0%
TATGAATGATTTCTGATACC+AGG + chr8_3:58711012-58711031 Msa1298060:intron 30.0%
!!! ATTTTAACTCTGATTCTCTC+TGG + chr8_3:58711037-58711056 Msa1298060:intron 30.0%
ATTTCTGATCCTTGATAAAG+AGG + chr8_3:58711406-58711425 Msa1298060:intron 30.0%
!! GGCAAATTTTTCAAAAACCA+AGG - chr8_3:58711459-58711478 None:intergenic 30.0%
TCATGATTTCTTCTACACTT+TGG - chr8_3:58711542-58711561 None:intergenic 30.0%
AAAGTGTAGAAGAAATCATG+AGG + chr8_3:58711541-58711560 Msa1298060:CDS 30.0%
ACTATGAAAATGTGTGATTC+AGG + chr8_3:58711676-58711695 Msa1298060:exon 30.0%
AATACAATGCCAACAGAAAA+GGG - chr8_3:58711730-58711749 None:intergenic 30.0%
AAATACAATGCCAACAGAAA+AGG - chr8_3:58711731-58711750 None:intergenic 30.0%
GAAATGTGTAAAATGAGTGC+AGG - chr8_3:58709595-58709614 None:intergenic 35.0%
ATAATAACTCATTGCGCCAA+GGG - chr8_3:58709702-58709721 None:intergenic 35.0%
AGGCTCTCAATAGTACAAAT+TGG - chr8_3:58710005-58710024 None:intergenic 35.0%
AGACAATGACTAGATGAGAA+GGG - chr8_3:58710083-58710102 None:intergenic 35.0%
AAGACAATGACTAGATGAGA+AGG - chr8_3:58710084-58710103 None:intergenic 35.0%
TTCTCATCTAGTCATTGTCT+TGG + chr8_3:58710083-58710102 Msa1298060:CDS 35.0%
TAACACCAGATATTAGCTGT+TGG - chr8_3:58710298-58710317 None:intergenic 35.0%
TGTTAGCTACTGTCATTCAA+TGG + chr8_3:58710313-58710332 Msa1298060:intron 35.0%
GAAATTGGAAAACACTCTCT+TGG + chr8_3:58710427-58710446 Msa1298060:CDS 35.0%
! GTTGAAAATTTTAAGCCGAG+GGG - chr8_3:58710465-58710484 None:intergenic 35.0%
TGTTATGAGCCATAACAAGT+GGG - chr8_3:58710738-58710757 None:intergenic 35.0%
TTGTTATGAGCCATAACAAG+TGG - chr8_3:58710739-58710758 None:intergenic 35.0%
TTGTATAGATTGCAGATGTG+TGG + chr8_3:58710863-58710882 Msa1298060:intron 35.0%
!! CTGAAATTTCTAGGATCTTC+TGG - chr8_3:58710943-58710962 None:intergenic 35.0%
GAATCAGAGTTAAAATTGCC+TGG - chr8_3:58711033-58711052 None:intergenic 35.0%
!! TCTGGTTGGTAGAGAATAAT+AGG + chr8_3:58711055-58711074 Msa1298060:intron 35.0%
ACTGAAATATGTACATCGTC+GGG - chr8_3:58711097-58711116 None:intergenic 35.0%
GACTGAAATATGTACATCGT+CGG - chr8_3:58711098-58711117 None:intergenic 35.0%
TACATATTTCAGTCGAGTGT+AGG + chr8_3:58711104-58711123 Msa1298060:CDS 35.0%
CATGGTAATTGCCTAAGATA+TGG + chr8_3:58711262-58711281 Msa1298060:intron 35.0%
!! TTAGAGGCATTTCATTACCA+CGG - chr8_3:58711308-58711327 None:intergenic 35.0%
AATGGTGATCCTCTTTATCA+AGG - chr8_3:58711418-58711437 None:intergenic 35.0%
TCAGCTTCAATTATCTCTTC+AGG - chr8_3:58711480-58711499 None:intergenic 35.0%
AGCTGAAAGAAAAGGTTATG+TGG + chr8_3:58711494-58711513 Msa1298060:CDS 35.0%
AGGTTATGTGGAAACAAAGA+AGG + chr8_3:58711506-58711525 Msa1298060:CDS 35.0%
ATTTCTTCTACACTTTGGCT+TGG - chr8_3:58711537-58711556 None:intergenic 35.0%
AGAACGTTATGAGCCATTAG+AGG + chr8_3:58709078-58709097 Msa1298060:CDS 40.0%
AATCAGCCATTAAAGGAACC+TGG + chr8_3:58709118-58709137 Msa1298060:CDS 40.0%
TTGCTCTCAAGTACATAGAG+AGG + chr8_3:58709182-58709201 Msa1298060:CDS 40.0%
TGCTCTCAAGTACATAGAGA+GGG + chr8_3:58709183-58709202 Msa1298060:CDS 40.0%
GTACATAGAGAGGGGTAAAA+AGG + chr8_3:58709192-58709211 Msa1298060:CDS 40.0%
TGTGGTTAATTATGTCCCTG+TGG - chr8_3:58709441-58709460 None:intergenic 40.0%
TAGGATGCCTTAATGATCTG+TGG - chr8_3:58709459-58709478 None:intergenic 40.0%
TGTATCATCTGTAGTTCCCT+TGG + chr8_3:58709683-58709702 Msa1298060:intron 40.0%
GATAATAACTCATTGCGCCA+AGG - chr8_3:58709703-58709722 None:intergenic 40.0%
GAATGAGATATCTGTTCTGC+AGG + chr8_3:58710047-58710066 Msa1298060:intron 40.0%
GACAATGACTAGATGAGAAG+GGG - chr8_3:58710082-58710101 None:intergenic 40.0%
! TGGTTGATTTAGTGAAGACG+AGG + chr8_3:58710149-58710168 Msa1298060:intron 40.0%
CTGTAGGAAATTTGTCATGC+AGG + chr8_3:58710401-58710420 Msa1298060:intron 40.0%
! TTGTCATGCAGGTCTGAAAT+TGG + chr8_3:58710412-58710431 Msa1298060:intron 40.0%
TTGGAAAACACTCTCTTGGA+TGG + chr8_3:58710431-58710450 Msa1298060:CDS 40.0%
! AAAATTTTAAGCCGAGGGGA+TGG - chr8_3:58710461-58710480 None:intergenic 40.0%
! TCCTTTTGTAACAGAACCTC+GGG - chr8_3:58710653-58710672 None:intergenic 40.0%
! CTCCTTTTGTAACAGAACCT+CGG - chr8_3:58710654-58710673 None:intergenic 40.0%
! CTGTTACAAAAGGAGTACGA+CGG + chr8_3:58710659-58710678 Msa1298060:CDS 40.0%
! GTTTCAAACCCCACTTGTTA+TGG + chr8_3:58710726-58710745 Msa1298060:intron 40.0%
GTTATGAGCCATAACAAGTG+GGG - chr8_3:58710737-58710756 None:intergenic 40.0%
GTTATGGCTCATAACAACCA+TGG + chr8_3:58710742-58710761 Msa1298060:intron 40.0%
! AACATAAAGTGTCACACCAC+AGG - chr8_3:58710890-58710909 None:intergenic 40.0%
!! GGATCTTCTGGTTCTTCAAA+TGG - chr8_3:58710931-58710950 None:intergenic 40.0%
TAACTCTGATTCTCTCTGGT+TGG + chr8_3:58711041-58711060 Msa1298060:intron 40.0%
AATATGTACATCGTCGGGTA+TGG - chr8_3:58711092-58711111 None:intergenic 40.0%
CAACAAAGATGCGAGATAGA+AGG - chr8_3:58711132-58711151 None:intergenic 40.0%
TGTTACATTGGCACATACCT+TGG - chr8_3:58711164-58711183 None:intergenic 40.0%
ATAAAGAGGATCACCATTCC+AGG + chr8_3:58711420-58711439 Msa1298060:intron 40.0%
TAAAGAGGATCACCATTCCA+GGG + chr8_3:58711421-58711440 Msa1298060:intron 40.0%
ATCTTCGATGTTCATGGAAC+CGG - chr8_3:58711631-58711650 None:intergenic 40.0%
ATGACGAGGAAATTGATGTC+AGG + chr8_3:58711649-58711668 Msa1298060:CDS 40.0%
TGACGAGGAAATTGATGTCA+GGG + chr8_3:58711650-58711669 Msa1298060:CDS 40.0%
GACATCCATTCACCAAATGA+AGG - chr8_3:58711702-58711721 None:intergenic 40.0%
! TGTCATTCTCCCTTTTCTGT+TGG + chr8_3:58711718-58711737 Msa1298060:three_prime_UTR 40.0%
TTGTCTTTAGCCAACCTAGC+AGG - chr8_3:58709145-58709164 None:intergenic 45.0%
GCTCTCAAGTACATAGAGAG+GGG + chr8_3:58709184-58709203 Msa1298060:CDS 45.0%
! TAGTGCTGCTGAGTGTGATT+AGG - chr8_3:58709287-58709306 None:intergenic 45.0%
TGTGGACACTCTCATCAATC+TGG - chr8_3:58709423-58709442 None:intergenic 45.0%
!! AGATTGATGAGAGTGTCCAC+AGG + chr8_3:58709422-58709441 Msa1298060:intron 45.0%
!! GATTGATGAGAGTGTCCACA+GGG + chr8_3:58709423-58709442 Msa1298060:intron 45.0%
! TGAAATGCGACCGACTCATA+AGG + chr8_3:58709653-58709672 Msa1298060:intron 45.0%
TCGTTGACGGGAAGTTAGTT+TGG + chr8_3:58709721-58709740 Msa1298060:intron 45.0%
ATTGTCTTGGAGTATGCTGC+TGG + chr8_3:58710096-58710115 Msa1298060:CDS 45.0%
TCTCAACCAAAGGCAACAGT+TGG + chr8_3:58710608-58710627 Msa1298060:CDS 45.0%
TCCTTCATACATTACTCCCG+AGG + chr8_3:58710634-58710653 Msa1298060:CDS 45.0%
ACCTCGGGAGTAATGTATGA+AGG - chr8_3:58710638-58710657 None:intergenic 45.0%
TCCCGAGGTTCTGTTACAAA+AGG + chr8_3:58710649-58710668 Msa1298060:CDS 45.0%
! AACAAGTGGGGTTTGAAACG+TGG - chr8_3:58710725-58710744 None:intergenic 45.0%
CACCATTCCAGGGATAAAAC+AGG + chr8_3:58711431-58711450 Msa1298060:CDS 45.0%
! GTCCTGTTTTATCCCTGGAA+TGG - chr8_3:58711436-58711455 None:intergenic 45.0%
!! CTTCTACACTTTGGCTTGGT+TGG - chr8_3:58711533-58711552 None:intergenic 45.0%
TGAGGATCATACAAGAAGCG+AGG + chr8_3:58711559-58711578 Msa1298060:CDS 45.0%
GAGGATCATACAAGAAGCGA+GGG + chr8_3:58711560-58711579 Msa1298060:CDS 45.0%
CTCGTCATCTTCGATGTTCA+TGG - chr8_3:58711637-58711656 None:intergenic 45.0%
CATGAACATCGAAGATGACG+AGG + chr8_3:58711635-58711654 Msa1298060:CDS 45.0%
GTGTGATTCAGGCCTTCATT+TGG + chr8_3:58711687-58711706 Msa1298060:exon 45.0%
! TCAGGCCTTCATTTGGTGAA+TGG + chr8_3:58711694-58711713 Msa1298060:three_prime_UTR 45.0%
GCAGGACCAGGTTCCTTTAA+TGG - chr8_3:58709127-58709146 None:intergenic 50.0%
TAAAGGAACCTGGTCCTGCT+AGG + chr8_3:58709128-58709147 Msa1298060:CDS 50.0%
! CTGGTGGTGAACTCTTCAAG+AGG + chr8_3:58710115-58710134 Msa1298060:CDS 50.0%
TTCAAGAGGATACGCACTGC+TGG + chr8_3:58710129-58710148 Msa1298060:CDS 50.0%
TGGATGGAAATCCATCCCCT+CGG + chr8_3:58710447-58710466 Msa1298060:CDS 50.0%
GGAGATCCAACTGTTGCCTT+TGG - chr8_3:58710617-58710636 None:intergenic 50.0%
! ACAAAAGGAGTACGACGGCA+AGG + chr8_3:58710664-58710683 Msa1298060:CDS 50.0%
ATTGCAGATGTGTGGTCCTG+TGG + chr8_3:58710871-58710890 Msa1298060:CDS 50.0%
ACATTGGCACATACCTTGGC+AGG - chr8_3:58711160-58711179 None:intergenic 50.0%
! CAAGGGTCCTGTTTTATCCC+TGG - chr8_3:58711441-58711460 None:intergenic 50.0%
CAGGGATAAAACAGGACCCT+TGG + chr8_3:58711439-58711458 Msa1298060:CDS 50.0%
! CATACAAGAAGCGAGGGTAC+CGG + chr8_3:58711566-58711585 Msa1298060:CDS 50.0%
!! ATACAAGAAGCGAGGGTACC+GGG + chr8_3:58711567-58711586 Msa1298060:CDS 50.0%
! CCAGCTTTTGATCCTTGTCC+CGG - chr8_3:58711588-58711607 None:intergenic 50.0%
CAAGGATCAAAAGCTGGCGA+TGG + chr8_3:58711591-58711610 Msa1298060:CDS 50.0%
TTAGCCAACCTAGCAGGACC+AGG - chr8_3:58709139-58709158 None:intergenic 55.0%
GTCTTGGAGTATGCTGCTGG+TGG + chr8_3:58710099-58710118 Msa1298060:CDS 55.0%
CTTTGTTGCCGATCCTGCCA+AGG + chr8_3:58711144-58711163 Msa1298060:CDS 55.0%
GGCACATACCTTGGCAGGAT+CGG - chr8_3:58711155-58711174 None:intergenic 55.0%
CCGGGACAAGGATCAAAAGC+TGG + chr8_3:58711585-58711604 Msa1298060:CDS 55.0%
GGATCAAAAGCTGGCGATGG+TGG + chr8_3:58711594-58711613 Msa1298060:CDS 55.0%
GGAACCTGGTCCTGCTAGGT+TGG + chr8_3:58709132-58709151 Msa1298060:CDS 60.0%
! GATGGTGGTCAAGCTGGCAC+CGG + chr8_3:58711609-58711628 Msa1298060:CDS 60.0%
!! GAAGCGAGGGTACCGGGACA+AGG + chr8_3:58711573-58711592 Msa1298060:CDS 65.0%
! GCTGGCGATGGTGGTCAAGC+TGG + chr8_3:58711603-58711622 Msa1298060:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 58709073 58711799 58709073 ID=Msa1298060;Name=Msa1298060
chr8_3 mRNA 58709073 58711799 58709073 ID=Msa1298060-mRNA-1;Parent=Msa1298060;Name=Msa1298060-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|0.85|0.62|1|1|1|8|107|312
chr8_3 exon 58709073 58709213 58709073 ID=Msa1298060-mRNA-1:exon:16880;Parent=Msa1298060-mRNA-1
chr8_3 exon 58709424 58709498 58709424 ID=Msa1298060-mRNA-1:exon:16881;Parent=Msa1298060-mRNA-1
chr8_3 exon 58710069 58710150 58710069 ID=Msa1298060-mRNA-1:exon:16882;Parent=Msa1298060-mRNA-1
chr8_3 exon 58710423 58710499 58710423 ID=Msa1298060-mRNA-1:exon:16883;Parent=Msa1298060-mRNA-1
chr8_3 exon 58710593 58710685 58710593 ID=Msa1298060-mRNA-1:exon:16884;Parent=Msa1298060-mRNA-1
chr8_3 exon 58710871 58710975 58710871 ID=Msa1298060-mRNA-1:exon:16885;Parent=Msa1298060-mRNA-1
chr8_3 exon 58711067 58711165 58711067 ID=Msa1298060-mRNA-1:exon:16886;Parent=Msa1298060-mRNA-1
chr8_3 exon 58711426 58711799 58711426 ID=Msa1298060-mRNA-1:exon:16887;Parent=Msa1298060-mRNA-1
chr8_3 CDS 58709073 58709213 58709073 ID=Msa1298060-mRNA-1:cds;Parent=Msa1298060-mRNA-1
chr8_3 CDS 58709424 58709498 58709424 ID=Msa1298060-mRNA-1:cds;Parent=Msa1298060-mRNA-1
chr8_3 CDS 58710069 58710150 58710069 ID=Msa1298060-mRNA-1:cds;Parent=Msa1298060-mRNA-1
chr8_3 CDS 58710423 58710499 58710423 ID=Msa1298060-mRNA-1:cds;Parent=Msa1298060-mRNA-1
chr8_3 CDS 58710593 58710685 58710593 ID=Msa1298060-mRNA-1:cds;Parent=Msa1298060-mRNA-1
chr8_3 CDS 58710871 58710975 58710871 ID=Msa1298060-mRNA-1:cds;Parent=Msa1298060-mRNA-1
chr8_3 CDS 58711067 58711165 58711067 ID=Msa1298060-mRNA-1:cds;Parent=Msa1298060-mRNA-1
chr8_3 CDS 58711426 58711692 58711426 ID=Msa1298060-mRNA-1:cds;Parent=Msa1298060-mRNA-1
chr8_3 three_prime_UTR 58711693 58711799 58711693 ID=Msa1298060-mRNA-1:three_prime_utr;Parent=Msa1298060-mRNA-1
Gene Sequence

>Msa1298060

ATGGAAGAACGTTATGAGCCATTAGAGGAACTTTTTTATTTTTTGAATCAGCCATTAAAGGAACCTGGTCCTGCTAGGTTGGCTAAAGACAAAAATACTGGCGAGCTTGTTGCTCTCAAGTACATAGAGAGGGGTAAAAAGATTGATGAGAGTGTCCACAGGGACATAATTAACCACAGATCATTAAGGCATCCTAATATTATCAGATTCAAAGAGGTCTTGCTCACCCCTTCTCATCTAGTCATTGTCTTGGAGTATGCTGCTGGTGGTGAACTCTTCAAGAGGATACGCACTGCTGGTCTGAAATTGGAAAACACTCTCTTGGATGGAAATCCATCCCCTCGGCTTAAAATTTTCAACTTTGATTACTCCAAGTCAACAATATTGCATTCTCAACCAAAGGCAACAGTTGGATCTCCTTCATACATTACTCCCGAGGTTCTGTTACAAAAGGAGTACGACGGCAAGATTGCAGATGTGTGGTCCTGTGGTGTGACACTTTATGTTATGCTAGTTGGTGCATATCCATTTGAAGAACCAGAAGATCCTAGAAATTTCAGAAAGACTATTGGGAGAATAATAGGTGTTCAATACTCCATACCCGACGATGTACATATTTCAGTCGAGTGTAGGAACCTTCTATCTCGCATCTTTGTTGCCGATCCTGCCAAGAGGATCACCATTCCAGGGATAAAACAGGACCCTTGGTTTTTGAAAAATTTGCCTGAAGAGATAATTGAAGCTGAAAGAAAAGGTTATGTGGAAACAAAGAAGGACCAACCAAGCCAAAGTGTAGAAGAAATCATGAGGATCATACAAGAAGCGAGGGTACCGGGACAAGGATCAAAAGCTGGCGATGGTGGTCAAGCTGGCACCGGTTCCATGAACATCGAAGATGACGAGGAAATTGATGTCAGGGATGACTATGAAAATGTGTGA
Protein sequence

>Msa1298060

MEERYEPLEELFYFLNQPLKEPGPARLAKDKNTGELVALKYIERGKKIDESVHRDIINHRSLRHPNIIRFKEVLLTPSHLVIVLEYAAGGELFKRIRTAGLKLENTLLDGNPSPRLKIFNFDYSKSTILHSQPKATVGSPSYITPEVLLQKEYDGKIADVWSCGVTLYVMLVGAYPFEEPEDPRNFRKTIGRIIGVQYSIPDDVHISVECRNLLSRIFVADPAKRITIPGIKQDPWFLKNLPEEIIEAERKGYVETKKDQPSQSVEEIMRIIQEARVPGQGSKAGDGGQAGTGSMNIEDDEEIDVRDDYENV*