AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0190750


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0190750 MtrunA17_Chr2g0280471 96.988 166 5 0 1 166 1 166 1.74e-118 331
Msa0190750 MtrunA17_Chr2g0280451 86.747 166 22 0 1 166 1 166 6.79e-105 297
Msa0190750 MtrunA17_Chr7g0249341 56.627 166 53 1 1 166 1 147 4.82e-64 193
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0190750 AT5G56260.1 75.301 166 41 0 1 166 1 166 1.61e-90 261
Msa0190750 AT5G56260.2 75.301 166 41 0 1 166 1 166 1.61e-90 261
Msa0190750 AT5G56260.5 75.301 166 41 0 1 166 1 166 1.61e-90 261
Msa0190750 AT5G56260.4 75.301 166 41 0 1 166 33 198 1.07e-89 260
Msa0190750 AT5G56260.3 75.301 166 41 0 1 166 33 198 1.07e-89 260
Msa0190750 AT3G02770.1 65.060 166 58 0 1 166 1 166 9.65e-78 229
Msa0190750 AT5G16450.2 65.060 166 58 0 1 166 1 166 1.48e-77 228
Msa0190750 AT5G16450.1 65.060 166 58 0 1 166 1 166 1.48e-77 228

Find 37 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCTTGTTGGAGGGAATT+TGG 0.199853 2_1:-6271482 Msa0190750:CDS
TGGCCAGTCTCGGGCATTCT+CGG 0.281731 2_1:-6271617 Msa0190750:CDS
GCACAAAACATGGGGTGGTC+TGG 0.304278 2_1:-6271451 Msa0190750:CDS
GGCCAGTCTCGGGCATTCTC+GGG 0.351904 2_1:-6271616 Msa0190750:CDS
TGGTCTGGGATTGTTGTGAA+TGG 0.381293 2_1:-6271436 Msa0190750:CDS
AAAAGGAGAAGGAAGAGTTT+TGG 0.387441 2_1:-6271533 Msa0190750:CDS
CACAAAACATGGGGTGGTCT+GGG 0.391877 2_1:-6271450 Msa0190750:CDS
CCCGAGACTGGCCATACATC+TGG 0.415145 2_1:+6271626 None:intergenic
GCCCCGAGAATGCCCGAGAC+TGG 0.420007 2_1:+6271614 None:intergenic
TCAAGTTGGTGTTAGAGCTT+TGG 0.422544 2_1:-6271380 Msa0190750:CDS
ACCTCTAGTACTATTTCAAA+TGG 0.429193 2_1:+6271219 None:intergenic
AACGCAACACATTTAACAAA+TGG 0.449158 2_1:-6271676 Msa0190750:CDS
TCCAGATGTATGGCCAGTCT+CGG 0.458278 2_1:-6271627 Msa0190750:CDS
GGTGCAGTTGGCACAAAACA+TGG 0.464361 2_1:-6271461 Msa0190750:CDS
CATCCAGTGTTCCAGATGTA+TGG 0.472302 2_1:-6271637 Msa0190750:CDS
TCAAATGGACAACTCAAACT+TGG 0.482072 2_1:+6271234 None:intergenic
TGGAGGGAATTTGGTGCAGT+TGG 0.484334 2_1:-6271473 Msa0190750:CDS
AGAGAACTTCTTGAAACAAA+AGG 0.491488 2_1:-6271550 Msa0190750:CDS
GAGATTAATTTGTGTCAAGT+TGG 0.491931 2_1:-6271394 Msa0190750:CDS
CATGTTGCTGTTTATGTTGG+AGG 0.502059 2_1:-6271313 Msa0190750:CDS
AAACATGTTGCTGTTTATGT+TGG 0.508506 2_1:-6271316 Msa0190750:CDS
TGGCCATACATCTGGAACAC+TGG 0.512769 2_1:+6271634 None:intergenic
CCAGATGTATGGCCAGTCTC+GGG 0.532810 2_1:-6271626 Msa0190750:CDS
GTGTCACATATCTCTGCTGT+TGG 0.545822 2_1:+6271700 None:intergenic
GCCAGTCTCGGGCATTCTCG+GGG 0.553955 2_1:-6271615 Msa0190750:CDS
AGCATGAGGTGTGCTCTTGT+TGG 0.557997 2_1:-6271493 Msa0190750:CDS
ATGAGGTGTGCTCTTGTTGG+AGG 0.569564 2_1:-6271490 Msa0190750:CDS
CTTCTTGAAACAAAAGGAGA+AGG 0.585218 2_1:-6271544 Msa0190750:CDS
TGGCTCTATGCAGATAATGA+TGG 0.590987 2_1:-6271268 Msa0190750:CDS
ATTTAACAAATGGTGACTTG+AGG 0.616446 2_1:-6271666 Msa0190750:CDS
AGTGTTTGAAGACAATGTGT+TGG 0.617013 2_1:-6271575 Msa0190750:CDS
GTGCAGTTGGCACAAAACAT+GGG 0.623381 2_1:-6271460 Msa0190750:CDS
GAATGGTTGTATTAGAGATG+TGG 0.623790 2_1:-6271419 Msa0190750:CDS
TGAGGTGTGCTCTTGTTGGA+GGG 0.651891 2_1:-6271489 Msa0190750:CDS
AGTTGGCACAAAACATGGGG+TGG 0.672135 2_1:-6271456 Msa0190750:CDS
TGCAGTTGGCACAAAACATG+GGG 0.672279 2_1:-6271459 Msa0190750:CDS
TTGATGGAGGAGGAAGCATG+AGG 0.673507 2_1:-6271507 Msa0190750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGTCTTCAAACACTTTTA+CGG + chr2_1:6271367-6271386 None:intergenic 25.0%
! CCACTTAAATCCAATAAAAA+CGG - chr2_1:6271597-6271616 Msa0190750:CDS 25.0%
AACGCAACACATTTAACAAA+TGG - chr2_1:6271270-6271289 Msa0190750:CDS 30.0%
ATTTAACAAATGGTGACTTG+AGG - chr2_1:6271280-6271299 Msa0190750:CDS 30.0%
AGAGAACTTCTTGAAACAAA+AGG - chr2_1:6271396-6271415 Msa0190750:CDS 30.0%
!! GAGATTAATTTGTGTCAAGT+TGG - chr2_1:6271552-6271571 Msa0190750:CDS 30.0%
!! CCGTTTTTATTGGATTTAAG+TGG + chr2_1:6271600-6271619 None:intergenic 30.0%
AAATCCAATAAAAACGGTAC+TGG - chr2_1:6271603-6271622 Msa0190750:CDS 30.0%
!! AAACATGTTGCTGTTTATGT+TGG - chr2_1:6271630-6271649 Msa0190750:CDS 30.0%
! AACACTTTTACGGTAACAAC+AGG + chr2_1:6271357-6271376 None:intergenic 35.0%
AGTGTTTGAAGACAATGTGT+TGG - chr2_1:6271371-6271390 Msa0190750:CDS 35.0%
CTTCTTGAAACAAAAGGAGA+AGG - chr2_1:6271402-6271421 Msa0190750:CDS 35.0%
! AAAAGGAGAAGGAAGAGTTT+TGG - chr2_1:6271413-6271432 Msa0190750:CDS 35.0%
!!! GGAAGAGTTTTGGTTATTGA+TGG - chr2_1:6271423-6271442 Msa0190750:CDS 35.0%
!!! AGAGTTTTGGTTATTGATGG+AGG - chr2_1:6271426-6271445 Msa0190750:CDS 35.0%
GAATGGTTGTATTAGAGATG+TGG - chr2_1:6271527-6271546 Msa0190750:CDS 35.0%
!! TTCACCAGTACCGTTTTTAT+TGG + chr2_1:6271610-6271629 None:intergenic 35.0%
!! CTTTTATTCGTGATGGAGAA+TGG - chr2_1:6271658-6271677 Msa0190750:CDS 35.0%
TCAAATGGACAACTCAAACT+TGG + chr2_1:6271715-6271734 None:intergenic 35.0%
!!! GTTTTGGTTATTGATGGAGG+AGG - chr2_1:6271429-6271448 Msa0190750:CDS 40.0%
!! TCAAGTTGGTGTTAGAGCTT+TGG - chr2_1:6271566-6271585 Msa0190750:CDS 40.0%
!! CATGTTGCTGTTTATGTTGG+AGG - chr2_1:6271633-6271652 Msa0190750:CDS 40.0%
! GGAGGAACTTTTATTCGTGA+TGG - chr2_1:6271651-6271670 Msa0190750:CDS 40.0%
! TGGCTCTATGCAGATAATGA+TGG - chr2_1:6271678-6271697 Msa0190750:CDS 40.0%
GTGTCACATATCTCTGCTGT+TGG + chr2_1:6271249-6271268 None:intergenic 45.0%
CATCCAGTGTTCCAGATGTA+TGG - chr2_1:6271309-6271328 Msa0190750:CDS 45.0%
TGCTCTTGTTGGAGGGAATT+TGG - chr2_1:6271464-6271483 Msa0190750:CDS 45.0%
GTGCAGTTGGCACAAAACAT+GGG - chr2_1:6271486-6271505 Msa0190750:CDS 45.0%
TGCAGTTGGCACAAAACATG+GGG - chr2_1:6271487-6271506 Msa0190750:CDS 45.0%
! TGGTCTGGGATTGTTGTGAA+TGG - chr2_1:6271510-6271529 Msa0190750:CDS 45.0%
! TGGCCATACATCTGGAACAC+TGG + chr2_1:6271315-6271334 None:intergenic 50.0%
TCCAGATGTATGGCCAGTCT+CGG - chr2_1:6271319-6271338 Msa0190750:CDS 50.0%
! TTGATGGAGGAGGAAGCATG+AGG - chr2_1:6271439-6271458 Msa0190750:CDS 50.0%
AGCATGAGGTGTGCTCTTGT+TGG - chr2_1:6271453-6271472 Msa0190750:CDS 50.0%
ATGAGGTGTGCTCTTGTTGG+AGG - chr2_1:6271456-6271475 Msa0190750:CDS 50.0%
TGAGGTGTGCTCTTGTTGGA+GGG - chr2_1:6271457-6271476 Msa0190750:CDS 50.0%
!! TGGAGGGAATTTGGTGCAGT+TGG - chr2_1:6271473-6271492 Msa0190750:CDS 50.0%
GGTGCAGTTGGCACAAAACA+TGG - chr2_1:6271485-6271504 Msa0190750:CDS 50.0%
AGTTGGCACAAAACATGGGG+TGG - chr2_1:6271490-6271509 Msa0190750:CDS 50.0%
CACAAAACATGGGGTGGTCT+GGG - chr2_1:6271496-6271515 Msa0190750:CDS 50.0%
CCAGATGTATGGCCAGTCTC+GGG - chr2_1:6271320-6271339 Msa0190750:CDS 55.0%
GCACAAAACATGGGGTGGTC+TGG - chr2_1:6271495-6271514 Msa0190750:CDS 55.0%
! CCCGAGACTGGCCATACATC+TGG + chr2_1:6271323-6271342 None:intergenic 60.0%
TGGCCAGTCTCGGGCATTCT+CGG - chr2_1:6271329-6271348 Msa0190750:CDS 60.0%
GGCCAGTCTCGGGCATTCTC+GGG - chr2_1:6271330-6271349 Msa0190750:CDS 65.0%
GCCAGTCTCGGGCATTCTCG+GGG - chr2_1:6271331-6271350 Msa0190750:CDS 65.0%
GCCCCGAGAATGCCCGAGAC+TGG + chr2_1:6271335-6271354 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 6271234 6271734 6271234 ID=Msa0190750;Name=Msa0190750
chr2_1 mRNA 6271234 6271734 6271234 ID=Msa0190750-mRNA-1;Parent=Msa0190750;Name=Msa0190750-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|-1|0|1|-1|1|1|0|166
chr2_1 exon 6271234 6271734 6271234 ID=Msa0190750-mRNA-1:exon:2844;Parent=Msa0190750-mRNA-1
chr2_1 CDS 6271234 6271734 6271234 ID=Msa0190750-mRNA-1:cds;Parent=Msa0190750-mRNA-1
Gene Sequence

>Msa0190750

ATGGATTCTCTACCAACAGCAGAGATATGTGACACAAACGCAACACATTTAACAAATGGTGACTTGAGGATACTTCATCCAGTGTTCCAGATGTATGGCCAGTCTCGGGCATTCTCGGGGCCTGTTGTTACCGTAAAAGTGTTTGAAGACAATGTGTTGGTAAGAGAACTTCTTGAAACAAAAGGAGAAGGAAGAGTTTTGGTTATTGATGGAGGAGGAAGCATGAGGTGTGCTCTTGTTGGAGGGAATTTGGTGCAGTTGGCACAAAACATGGGGTGGTCTGGGATTGTTGTGAATGGTTGTATTAGAGATGTGGATGAGATTAATTTGTGTCAAGTTGGTGTTAGAGCTTTGGCTTCTCATCCACTTAAATCCAATAAAAACGGTACTGGTGAAAAACATGTTGCTGTTTATGTTGGAGGAACTTTTATTCGTGATGGAGAATGGCTCTATGCAGATAATGATGGTATCATTGTATCCAAGTTTGAGTTGTCCATTTGA

Protein sequence

>Msa0190750

MDSLPTAEICDTNATHLTNGDLRILHPVFQMYGQSRAFSGPVVTVKVFEDNVLVRELLETKGEGRVLVIDGGGSMRCALVGGNLVQLAQNMGWSGIVVNGCIRDVDEINLCQVGVRALASHPLKSNKNGTGEKHVAVYVGGTFIRDGEWLYADNDGIIVSKFELSI*