AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1425530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1425530 MtrunA17_Chr4g0006181 99.308 289 2 0 1 289 59 347 0.0 590
Msa1425530 MtrunA17_Chr3g0113311 70.566 265 74 1 12 272 11 275 6.70e-134 380
Msa1425530 MtrunA17_Chr6g0462031 40.000 230 90 5 57 282 49 234 1.80e-44 151
Msa1425530 MtrunA17_Chr2g0301081 34.615 260 92 7 53 288 61 266 1.12e-38 137
Msa1425530 MtrunA17_Chr8g0345631 33.205 259 95 5 53 287 62 266 7.23e-35 127
Msa1425530 MtrunA17_Chr3g0133901 30.795 302 131 5 13 289 17 265 9.39e-35 126
Msa1425530 MtrunA17_Chr6g0476641 33.452 281 116 10 35 289 47 282 1.42e-34 126
Msa1425530 MtrunA17_Chr3g0092091 30.671 313 132 8 1 289 1 252 2.10e-31 117
Msa1425530 MtrunA17_Chr6g0453181 33.446 296 136 10 3 281 2 253 5.51e-31 116
Msa1425530 MtrunA17_Chr4g0051941 34.228 298 132 11 3 281 2 254 1.90e-30 115
Msa1425530 MtrunA17_Chr6g0453141 33.670 297 134 11 3 281 2 253 1.02e-29 113
Msa1425530 MtrunA17_Chr6g0453151 33.670 297 134 11 3 281 69 320 2.23e-29 114
Msa1425530 MtrunA17_Chr6g0453161 32.770 296 138 10 3 281 269 520 2.71e-29 116
Msa1425530 MtrunA17_Chr6g0453161 43.293 164 78 7 132 281 93 255 3.78e-27 110
Msa1425530 MtrunA17_Chr2g0318521 35.622 233 92 7 61 278 60 249 3.25e-29 112
Msa1425530 MtrunA17_Chr1g0157481 31.111 315 139 12 5 288 62 329 9.31e-29 112
Msa1425530 MtrunA17_Chr2g0279001 31.818 308 115 9 4 268 2 257 9.52e-29 110
Msa1425530 MtrunA17_Chr5g0447531 32.836 268 109 11 35 278 30 250 1.56e-26 104
Msa1425530 MtrunA17_Chr5g0448421 29.710 276 122 7 39 286 43 274 1.46e-25 102
Msa1425530 MtrunA17_Chr5g0448411 30.000 280 121 7 35 286 40 272 2.08e-25 102
Msa1425530 MtrunA17_Chr6g0464511 46.018 113 54 4 175 281 89 200 5.19e-20 86.3
Msa1425530 MtrunA17_Chr6g0453171 56.716 67 28 1 215 281 21 86 1.64e-18 79.0
Msa1425530 MtrunA17_Chr2g0296081 50.000 72 31 2 216 287 36 102 4.59e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1425530 AT3G08940.2 87.004 277 33 2 14 289 13 287 2.49e-178 493
Msa1425530 AT5G01530.1 87.726 277 33 1 14 289 14 290 3.49e-174 483
Msa1425530 AT2G40100.1 70.189 265 74 2 12 272 10 273 1.30e-132 377
Msa1425530 AT3G08940.1 77.326 172 36 2 15 185 14 183 4.75e-90 267
Msa1425530 AT2G40100.2 64.024 164 55 1 12 171 10 173 3.32e-71 218
Msa1425530 AT3G54890.1 41.453 234 87 6 57 285 48 236 5.05e-47 158
Msa1425530 AT1G19150.1 33.846 260 94 6 53 288 64 269 5.50e-35 127
Msa1425530 AT4G10340.1 35.573 253 102 8 53 289 66 273 9.23e-35 127
Msa1425530 AT3G61470.1 33.977 259 93 6 53 287 51 255 1.32e-34 126
Msa1425530 AT1G45474.2 32.830 265 99 5 51 289 43 254 2.13e-34 125
Msa1425530 AT1G45474.1 32.830 265 99 5 51 289 43 254 2.13e-34 125
Msa1425530 AT2G34420.1 34.333 300 128 11 3 281 2 253 8.75e-33 121
Msa1425530 AT1G29930.1 35.099 302 125 13 3 281 2 255 1.78e-32 120
Msa1425530 AT1G29920.1 35.099 302 125 13 3 281 2 255 3.71e-32 120
Msa1425530 AT1G29910.1 35.099 302 125 13 3 281 2 255 3.71e-32 120
Msa1425530 AT1G76570.1 34.304 309 126 12 5 283 61 322 4.21e-32 121
Msa1425530 AT3G47470.1 30.645 310 129 8 4 287 2 251 7.65e-32 119
Msa1425530 AT3G54890.4 37.629 194 72 5 57 246 48 196 1.53e-30 114
Msa1425530 AT5G54270.1 33.696 276 108 8 35 278 18 250 1.99e-30 115
Msa1425530 AT2G34430.1 44.785 163 74 6 134 281 93 254 6.18e-30 114
Msa1425530 AT1G15820.1 33.704 270 75 9 40 268 48 254 4.47e-29 111
Msa1425530 AT1G61520.3 30.435 276 119 7 39 286 40 270 6.34e-28 108
Msa1425530 AT1G61520.1 30.435 276 119 7 39 286 40 270 6.34e-28 108
Msa1425530 AT2G05070.1 33.108 296 125 13 7 278 4 250 2.38e-27 107
Msa1425530 AT2G05100.1 34.363 259 96 11 47 278 39 250 1.84e-26 105
Msa1425530 AT3G27690.2 33.110 299 130 13 3 278 32 283 3.14e-26 105
Msa1425530 AT3G27690.1 33.220 295 127 13 7 278 4 251 7.83e-26 103
Msa1425530 AT1G61520.2 29.388 245 102 6 70 286 14 215 2.01e-20 87.8
Msa1425530 AT2G05100.2 32.927 246 91 11 47 265 39 237 2.41e-19 86.7
Msa1425530 AT5G28450.1 45.783 83 44 1 206 287 89 171 6.29e-14 68.9

Find 178 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTTCGACCTAATGTTTA+AGG 0.157400 tig0025867:+22984 None:intergenic
CTTGATCTTGATGGTTTCTT+TGG 0.159376 tig0025867:+25236 None:intergenic
GAGTTTCTCGCGCGTCTTAT+TGG 0.196650 tig0025867:-23233 Msa1425530:three_prime_UTR
GTTGGAAGGTATGTTCATTT+TGG 0.214618 tig0025867:-22637 Msa1425530:three_prime_UTR
CTTACTGTTGAATGGCTTAC+TGG 0.215175 tig0025867:-24616 Msa1425530:CDS
CAGCCTTACAGTGAAGTATT+TGG 0.222406 tig0025867:-24706 Msa1425530:CDS
AACTTGTTACCCAAACAAAA+TGG 0.225218 tig0025867:-23008 Msa1425530:three_prime_UTR
TATTTGGACTTCAGAGGTTT+AGG 0.232272 tig0025867:-24690 Msa1425530:CDS
CGACTCGCTTGATCAGAATT+TGG 0.240362 tig0025867:-24797 Msa1425530:CDS
AGTTTCTCGCGCGTCTTATT+GGG 0.265119 tig0025867:-23232 Msa1425530:three_prime_UTR
GAATAACTTGTTAGAGGATT+TGG 0.276097 tig0025867:-22748 Msa1425530:three_prime_UTR
TGGTGATGAATTGGTGATTA+TGG 0.278938 tig0025867:+25483 None:intergenic
GGAAGTTTGGTCGGAGATTA+CGG 0.284629 tig0025867:-24871 Msa1425530:CDS
GGCTGCAGCGGTGGCGGTTG+CGG 0.291908 tig0025867:+25352 None:intergenic
CTATATATATAAATGCTATT+TGG 0.296999 tig0025867:-23432 Msa1425530:three_prime_UTR
TGGATCGAAGTTCTAGTAAT+TGG 0.298063 tig0025867:-23759 Msa1425530:CDS
GGATTTGATCCATTTGGTTT+GGG 0.302182 tig0025867:-24850 Msa1425530:CDS
CAAAACAAGTTAGAACATTT+CGG 0.309667 tig0025867:+23199 None:intergenic
TCTCTATCACAACACTTATC+TGG 0.311866 tig0025867:-23779 Msa1425530:CDS
AGCGACGGTGGCGGAGGTTT+GGG 0.324777 tig0025867:-22844 Msa1425530:three_prime_UTR
CGGATTTGATCCATTTGGTT+TGG 0.331501 tig0025867:-24851 Msa1425530:CDS
TGGCGGTTGCGGCGGCCATT+TGG 0.332788 tig0025867:+25363 None:intergenic
AAGCGACGGTGGCGGAGGTT+TGG 0.333824 tig0025867:-22845 Msa1425530:three_prime_UTR
GTGAAATTCCTAATGTGAAT+TGG 0.346762 tig0025867:-23369 Msa1425530:three_prime_UTR
TGGTGATTATGGATAATCTT+TGG 0.353186 tig0025867:+25494 None:intergenic
ACTTCACTGTAAGGCTGAAA+TGG 0.355377 tig0025867:+24712 None:intergenic
CTTGCTATGGTTGCTTTCCT+TGG 0.358398 tig0025867:-23594 Msa1425530:CDS
TTTATTGAATAACTTGTTAG+AGG 0.360410 tig0025867:-22754 Msa1425530:three_prime_UTR
ATTTGGACTTCAGAGGTTTA+GGG 0.363642 tig0025867:-24689 Msa1425530:CDS
GTAAAGGACCACTTAACAAT+TGG 0.364076 tig0025867:-23545 Msa1425530:CDS
CACTCCGGTGACTTTGCTCC+GGG 0.367189 tig0025867:+24901 None:intergenic
TCTTCTTTCCAAAGCCGAAT+CGG 0.368119 tig0025867:+25267 None:intergenic
GGCTTGGATCCTACCCGAAT+TGG 0.370873 tig0025867:+25289 None:intergenic
AAACATTAGGTCGAAGTGTT+GGG 0.371629 tig0025867:-22980 Msa1425530:three_prime_UTR
TTTCAGGTTGAGCTAGTAGA+AGG 0.373969 tig0025867:-23831 Msa1425530:intron
CAAAATGGGCCTTAAACATT+AGG 0.374408 tig0025867:-22993 Msa1425530:three_prime_UTR
TTCCAAAGCCGAATCGGGCT+TGG 0.378185 tig0025867:+25273 None:intergenic
ATCCAAGCCCGATTCGGCTT+TGG 0.380468 tig0025867:-25275 Msa1425530:CDS
TTTGCTCCGGGGTACCATAA+TGG 0.383572 tig0025867:+24913 None:intergenic
TAACTGCTCCAATTCACATT+AGG 0.383713 tig0025867:+23361 None:intergenic
GTTCTTGATTAACTCATGTA+TGG 0.384423 tig0025867:-23463 Msa1425530:three_prime_UTR
TGAGGGCAATGAGGAACATT+AGG 0.388001 tig0025867:-23277 Msa1425530:three_prime_UTR
CTTCTTTCCAAAGCCGAATC+GGG 0.391044 tig0025867:+25268 None:intergenic
GTGAGTTGATTCATGGTAGA+TGG 0.391291 tig0025867:-24663 Msa1425530:CDS
GATTACGGATTTGATCCATT+TGG 0.397788 tig0025867:-24856 Msa1425530:CDS
AGGGAATGTGAGTTGATTCA+TGG 0.400554 tig0025867:-24670 Msa1425530:CDS
TTGATGTGGTGGTGATGAAT+TGG 0.401399 tig0025867:+25474 None:intergenic
ATGAGGAGGATGAGTGGCTT+TGG 0.404388 tig0025867:-22707 Msa1425530:three_prime_UTR
GTGGCGGAGGTTTGGGTGAA+TGG 0.404667 tig0025867:-22837 Msa1425530:three_prime_UTR
AAAGAAAGCAACACTTCAAT+TGG 0.404848 tig0025867:-23637 Msa1425530:CDS
CCGCGAAGTTCAATCCAATT+CGG 0.405011 tig0025867:-25303 Msa1425530:CDS
CGCGAAGTTCAATCCAATTC+GGG 0.412313 tig0025867:-25302 Msa1425530:CDS
AAGACGCGCGAGAAACTCAT+AGG 0.413072 tig0025867:+23238 None:intergenic
GTTTGGGTGAATGGCGAGAT+GGG 0.414370 tig0025867:-22828 Msa1425530:three_prime_UTR
TTGGTGCTCTTACTGTTGAA+TGG 0.414634 tig0025867:-24624 Msa1425530:CDS
CATCAAGCTCAGCATTTCTC+TGG 0.414677 tig0025867:+23724 None:intergenic
GTACTGGGACTTGATCTTGA+TGG 0.416331 tig0025867:+25227 None:intergenic
ATTATGGATAATCTTTGGTT+TGG 0.423165 tig0025867:+25499 None:intergenic
GCTGTTCAAGCTGCTGCTAC+TGG 0.427306 tig0025867:-23567 Msa1425530:CDS
AGGATTTGGAGGGTCATGTT+GGG 0.427442 tig0025867:-22734 Msa1425530:three_prime_UTR
GTAGAAGGATCAACATATCT+TGG 0.431470 tig0025867:-23816 Msa1425530:CDS
CCACTCCGGTGACTTTGCTC+CGG 0.432695 tig0025867:+24900 None:intergenic
TGGGCTATGCTTGCTACTCT+TGG 0.438475 tig0025867:-24643 Msa1425530:CDS
GATTCACCCTCGTGGGAATC+CGG 0.439616 tig0025867:+22514 None:intergenic
CTTTGGAAGTTAGAAGAGGA+TGG 0.439791 tig0025867:-22690 Msa1425530:three_prime_UTR
TAAATCTATGCATACTAAGT+TGG 0.441664 tig0025867:-22655 Msa1425530:three_prime_UTR
CCGGAGATGTGATCGGAACT+AGG 0.444206 tig0025867:-24762 Msa1425530:CDS
GGAGTGGCTAGACGGAAGTT+TGG 0.446982 tig0025867:-24884 Msa1425530:CDS
CAAGCTGCTGCTACTGGTAA+AGG 0.448841 tig0025867:-23561 Msa1425530:CDS
GGTAGGATCCAAGCCCGATT+CGG 0.454874 tig0025867:-25281 Msa1425530:CDS
GGAAAATTCTTTGACCCACT+TGG 0.458494 tig0025867:-23678 Msa1425530:CDS
CGATAACGCCACTGCCTTCG+AGG 0.458569 tig0025867:+22554 None:intergenic
GGGTGAATGGCGAGATGGGA+AGG 0.460424 tig0025867:-22824 Msa1425530:three_prime_UTR
AGGGCGCGCAAGAAATCAAT+AGG 0.461474 tig0025867:-23124 Msa1425530:three_prime_UTR
AATTTGGCGAAGAATCTTGC+CGG 0.462568 tig0025867:-24781 Msa1425530:CDS
TTGACTAAGCATGAAATTTG+AGG 0.467015 tig0025867:-23295 Msa1425530:three_prime_UTR
AATGTGAATTGGAGCAGTTA+AGG 0.470078 tig0025867:-23358 Msa1425530:three_prime_UTR
CAGGTACTGATCGTCCATTA+TGG 0.471652 tig0025867:-24927 Msa1425530:CDS
ATATAAATGCTATTTGGCAT+TGG 0.474581 tig0025867:-23426 Msa1425530:three_prime_UTR
TTATATAATCTGAACTACAT+AGG 0.475676 tig0025867:-23144 Msa1425530:three_prime_UTR
TGGATTGAACTTCGCGGAGG+CGG 0.476462 tig0025867:+25309 None:intergenic
ACTTGTTACCCAAACAAAAT+GGG 0.477726 tig0025867:-23007 Msa1425530:three_prime_UTR
ATAAGTGTTGTGATAGAGAA+TGG 0.479242 tig0025867:+23783 None:intergenic
GAGGATTTGGAGGGTCATGT+TGG 0.481391 tig0025867:-22735 Msa1425530:three_prime_UTR
TGGTTTATGCTAATGTGAGC+AGG 0.482884 tig0025867:+25383 None:intergenic
GGAAGAAAAGGTATCAATGA+TGG 0.486648 tig0025867:+23491 None:intergenic
TGCAGCGGTGGCGGTTGCGG+CGG 0.492945 tig0025867:+25355 None:intergenic
TAAACATTAGGTCGAAGTGT+TGG 0.493781 tig0025867:-22981 Msa1425530:three_prime_UTR
ATCAATAGGATGAGAAAAGG+AGG 0.493891 tig0025867:-23110 Msa1425530:three_prime_UTR
TGACTAAGCATGAAATTTGA+GGG 0.498032 tig0025867:-23294 Msa1425530:three_prime_UTR
ATGGCGAGATGGGAAGGGAG+TGG 0.499769 tig0025867:-22818 Msa1425530:three_prime_UTR
CAAGAACTAAGAGGAAGAAA+AGG 0.500205 tig0025867:+23479 None:intergenic
GAAATCAATAGGATGAGAAA+AGG 0.501625 tig0025867:-23113 Msa1425530:three_prime_UTR
TAGACAAGTGTGGAAGAGTC+CGG 0.503085 tig0025867:-22580 Msa1425530:three_prime_UTR
GTTACATAATAATGTTAAGA+TGG 0.504422 tig0025867:+23028 None:intergenic
GCTGGTTTCCCCAAACCAAA+TGG 0.504445 tig0025867:+24841 None:intergenic
GGATTGAACTTCGCGGAGGC+GGG 0.511648 tig0025867:+25310 None:intergenic
TAGTGTTGTATTTGATGTGG+TGG 0.521205 tig0025867:+25463 None:intergenic
AAAAGAAGCGACGGTGGCGG+AGG 0.523348 tig0025867:-22850 Msa1425530:three_prime_UTR
CCTAGTTCCGATCACATCTC+CGG 0.525368 tig0025867:+24762 None:intergenic
TAAAGGACCACTTAACAATT+GGG 0.525889 tig0025867:-23544 Msa1425530:CDS
GGTGTTACATGGCAAGATGC+TGG 0.526008 tig0025867:-24595 Msa1425530:CDS
GGAAGTTAGAAGAGGATGGG+AGG 0.526262 tig0025867:-22686 Msa1425530:three_prime_UTR
TAACTTGTTAGAGGATTTGG+AGG 0.528716 tig0025867:-22745 Msa1425530:three_prime_UTR
TTTGGATAACAAGTGGGGTA+CGG 0.530717 tig0025867:-22619 Msa1425530:three_prime_UTR
AGTCCAAATACTTCACTGTA+AGG 0.530759 tig0025867:+24703 None:intergenic
TGAAGTGGCTGCAGCGGTGG+CGG 0.531682 tig0025867:+25346 None:intergenic
TATATAATCTGAACTACATA+GGG 0.533030 tig0025867:-23143 Msa1425530:three_prime_UTR
TTTGGAAGTTAGAAGAGGAT+GGG 0.534072 tig0025867:-22689 Msa1425530:three_prime_UTR
TGAGTAGCCCAATTGTTAAG+TGG 0.534802 tig0025867:+23537 None:intergenic
GCCACTGCCTTCGAGGGTGC+CGG 0.535719 tig0025867:+22561 None:intergenic
TCACATTAGCATAAACCAAA+TGG 0.537826 tig0025867:-25378 Msa1425530:exon
TGAGTTAATCAAGAACTAAG+AGG 0.538848 tig0025867:+23470 None:intergenic
GGTTTGGGTGAATGGCGAGA+TGG 0.539811 tig0025867:-22829 Msa1425530:three_prime_UTR
TCCGGCACCCTCGAAGGCAG+TGG 0.540281 tig0025867:-22562 Msa1425530:three_prime_UTR
AATGGCTTACTGGTGTTACA+TGG 0.542824 tig0025867:-24606 Msa1425530:CDS
GGTGAATGGCGAGATGGGAA+GGG 0.543748 tig0025867:-22823 Msa1425530:three_prime_UTR
CAAACATGCTCGTCTTGCTA+TGG 0.544135 tig0025867:-23607 Msa1425530:CDS
GAAGGGAGTGGAGGTTTATG+TGG 0.545521 tig0025867:-22806 Msa1425530:three_prime_UTR
GGGTCATGTTGGGTCTCATG+AGG 0.550259 tig0025867:-22724 Msa1425530:three_prime_UTR
CCGAATTGGATTGAACTTCG+CGG 0.551574 tig0025867:+25303 None:intergenic
TCAAATTGCAAATACTCCGC+TGG 0.554662 tig0025867:+24823 None:intergenic
GAAGAGTCCGGCACCCTCGA+AGG 0.556658 tig0025867:-22568 Msa1425530:three_prime_UTR
AACATTAGGTCGAAGTGTTG+GGG 0.556714 tig0025867:-22979 Msa1425530:three_prime_UTR
TGGCTAGACGGAAGTTTGGT+CGG 0.562317 tig0025867:-24880 Msa1425530:CDS
AGATGTGATCGGAACTAGGA+CGG 0.563578 tig0025867:-24758 Msa1425530:CDS
GATTTGATCCATTTGGTTTG+GGG 0.565681 tig0025867:-24849 Msa1425530:CDS
GTGGCTTTGGAAGTTAGAAG+AGG 0.565781 tig0025867:-22694 Msa1425530:three_prime_UTR
GTGAAGTATTTGGACTTCAG+AGG 0.565781 tig0025867:-24696 Msa1425530:CDS
TGATAGTGTTGTATTTGATG+TGG 0.569485 tig0025867:+25460 None:intergenic
CGAAGGCAGTGGCGTTATCG+TGG 0.572000 tig0025867:-22551 Msa1425530:three_prime_UTR
GCGAGATGGGAAGGGAGTGG+AGG 0.579451 tig0025867:-22815 Msa1425530:three_prime_UTR
TGATGAAGTGGCTGCAGCGG+TGG 0.581298 tig0025867:+25343 None:intergenic
GGTCTCATGAGGAGGATGAG+TGG 0.584966 tig0025867:-22713 Msa1425530:three_prime_UTR
GATAACGCCACTGCCTTCGA+GGG 0.585897 tig0025867:+22555 None:intergenic
AATCTTGCCGGAGATGTGAT+CGG 0.588189 tig0025867:-24769 Msa1425530:CDS
AGGATGAGAAAAGGAGGAGT+CGG 0.592384 tig0025867:-23104 Msa1425530:three_prime_UTR
CACCTTAAAAGAAGCGACGG+TGG 0.597352 tig0025867:-22856 Msa1425530:three_prime_UTR
CTTAAAAGAAGCGACGGTGG+CGG 0.597860 tig0025867:-22853 Msa1425530:three_prime_UTR
TGAGTTGATTCATGGTAGAT+GGG 0.603160 tig0025867:-24662 Msa1425530:CDS
ATTTGGTTTGGGGAAACCAG+CGG 0.603481 tig0025867:-24839 Msa1425530:CDS
TCTATGCATACTAAGTTGGA+AGG 0.603716 tig0025867:-22651 Msa1425530:three_prime_UTR
TACATGGCAAGATGCTGGAA+AGG 0.603751 tig0025867:-24590 Msa1425530:intron
CATGAAATTTGAGGGCAATG+AGG 0.607187 tig0025867:-23286 Msa1425530:three_prime_UTR
AGTGGAGGTTTATGTGGCGG+CGG 0.609678 tig0025867:-22800 Msa1425530:three_prime_UTR
AAGTCACCGGAGTGGCTAGA+CGG 0.609847 tig0025867:-24892 Msa1425530:CDS
CGCGCGAGAAACTCATAGGG+TGG 0.610162 tig0025867:+23242 None:intergenic
GGTTTATGCTAATGTGAGCA+GGG 0.610931 tig0025867:+25384 None:intergenic
GCAGCCACTTCATCATTCAT+CGG 0.611200 tig0025867:-25335 Msa1425530:CDS
GTTGTGATAGAGAATGGAAG+TGG 0.611749 tig0025867:+23789 None:intergenic
AAACTTCCGTCTAGCCACTC+CGG 0.616068 tig0025867:+24886 None:intergenic
GTACCCCGGAGCAAAGTCAC+CGG 0.620014 tig0025867:-24905 Msa1425530:CDS
TCTTGATGGTTTCTTTGGTG+CGG 0.621232 tig0025867:+25241 None:intergenic
GGGAGTGGAGGTTTATGTGG+CGG 0.622216 tig0025867:-22803 Msa1425530:three_prime_UTR
GTGGGGTACGGAGGAGAAAG+CGG 0.624525 tig0025867:-22607 Msa1425530:three_prime_UTR
TCATGTTGGGTCTCATGAGG+AGG 0.628294 tig0025867:-22721 Msa1425530:three_prime_UTR
TCAATGATGGTAGTGTGAAG+AGG 0.631393 tig0025867:+23504 None:intergenic
GGTCAGATGCTAAGCCAAGT+GGG 0.631916 tig0025867:+23664 None:intergenic
GCATTGGTTTGTAAACATTG+TGG 0.633055 tig0025867:-23410 Msa1425530:three_prime_UTR
GGGTCAGATGCTAAGCCAAG+TGG 0.635931 tig0025867:+23663 None:intergenic
CAGCTTGAACAGCAAAACCA+AGG 0.638361 tig0025867:+23577 None:intergenic
GGATAACAAGTGGGGTACGG+AGG 0.640840 tig0025867:-22616 Msa1425530:three_prime_UTR
AGACGCGCGAGAAACTCATA+GGG 0.640977 tig0025867:+23239 None:intergenic
TACTTGACCGGATTCCCACG+AGG 0.641748 tig0025867:-22521 None:intergenic
ACTTGACCGGATTCCCACGA+GGG 0.643385 tig0025867:-22520 None:intergenic
AAACACCTTAAAAGAAGCGA+CGG 0.648870 tig0025867:-22859 Msa1425530:three_prime_UTR
GAATGATGAAGTGGCTGCAG+CGG 0.651301 tig0025867:+25340 None:intergenic
AAGTTCAATCCAATTCGGGT+AGG 0.656773 tig0025867:-25298 Msa1425530:CDS
GGTGCCGATGAATGATGAAG+TGG 0.658626 tig0025867:+25331 None:intergenic
AAGCGGTGTTTAGACAAGTG+TGG 0.666298 tig0025867:-22590 Msa1425530:three_prime_UTR
GATCGTCCATTATGGTACCC+CGG 0.673771 tig0025867:-24919 Msa1425530:CDS
CCGGAGCAAAGTCACCGGAG+TGG 0.687601 tig0025867:-24900 Msa1425530:CDS
AACTTGTTAGAGGATTTGGA+GGG 0.691679 tig0025867:-22744 Msa1425530:three_prime_UTR
ACTCCGGTGACTTTGCTCCG+GGG 0.699763 tig0025867:+24902 None:intergenic
AATTGGATTGAACTTCGCGG+AGG 0.719272 tig0025867:+25306 None:intergenic
GAAAAGAGACTATACCCAGG+TGG 0.723166 tig0025867:-23699 Msa1425530:CDS
GCAGAAAAGAGACTATACCC+AGG 0.746677 tig0025867:-23702 Msa1425530:CDS
TTATGCTAATGTGAGCAGGG+TGG 0.828916 tig0025867:+25387 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATTTTTTTTATTTTT+AGG - tig0025867:23646-23665 Msa1425530:CDS 0.0%
!!! TTTTATTTTTTTTATTTTTA+GGG - tig0025867:23647-23666 Msa1425530:CDS 0.0%
!!! TTTTTCTTTTTACATTATAT+TGG - tig0025867:23984-24003 Msa1425530:intron 10.0%
!! AAAGAAGATTAAAAAATTAT+TGG - tig0025867:24018-24037 Msa1425530:intron 10.0%
!! TATTAGTTATAATTAGTTGA+TGG - tig0025867:22890-22909 Msa1425530:three_prime_UTR 15.0%
!! AATCTTTAATTATATCTAAC+AGG - tig0025867:23082-23101 Msa1425530:three_prime_UTR 15.0%
!!! CATGTTTTATGTTTTTTTTA+TGG - tig0025867:23698-23717 Msa1425530:CDS 15.0%
!!! AAATCTTACTAAACTATTTT+AGG + tig0025867:23844-23863 None:intergenic 15.0%
!! TTTCATTATCATATAATATG+AGG - tig0025867:24100-24119 Msa1425530:intron 15.0%
!!! TTCACTAAATTATGAAATTT+TGG - tig0025867:24167-24186 Msa1425530:intron 15.0%
!!! AAATTTTGGTATAATTTTTC+AGG - tig0025867:24181-24200 Msa1425530:intron 15.0%
!! CTATATATATAAATGCTATT+TGG - tig0025867:24596-24615 Msa1425530:CDS 15.0%
!! TTAATTATATCTAACGTACT+GGG + tig0025867:22819-22838 None:intergenic 20.0%
!! TTTAATTATATCTAACGTAC+TGG + tig0025867:22820-22839 None:intergenic 20.0%
!! AAAAAAGAATATCATACTAG+TGG - tig0025867:22982-23001 Msa1425530:three_prime_UTR 20.0%
!!! TTTATGTTTGTTTTGTATAG+AGG - tig0025867:23766-23785 Msa1425530:CDS 20.0%
!! ATTACAAACATTATATCACA+TGG - tig0025867:23892-23911 Msa1425530:intron 20.0%
!!! AAGTAAGAAAATAGGTTTTA+TGG - tig0025867:23917-23936 Msa1425530:intron 20.0%
!!! TTAATATTTTTTCTGCTAGA+TGG - tig0025867:24693-24712 Msa1425530:CDS 20.0%
!!! TAATATTTTTTCTGCTAGAT+GGG - tig0025867:24694-24713 Msa1425530:CDS 20.0%
!! TTATATAATCTGAACTACAT+AGG - tig0025867:24884-24903 Msa1425530:CDS 20.0%
!! TATATAATCTGAACTACATA+GGG - tig0025867:24885-24904 Msa1425530:CDS 20.0%
!! GTTACATAATAATGTTAAGA+TGG + tig0025867:25003-25022 None:intergenic 20.0%
!!! TTTATTGAATAACTTGTTAG+AGG - tig0025867:25274-25293 Msa1425530:CDS 20.0%
! ATTATGGATAATCTTTGGTT+TGG + tig0025867:22532-22551 None:intergenic 25.0%
!! GTTTGTGAGTTTTTGAATTT+GGG + tig0025867:22599-22618 None:intergenic 25.0%
!! TGTTTGTGAGTTTTTGAATT+TGG + tig0025867:22600-22619 None:intergenic 25.0%
!! AGTAACTAACTTGTGTAAAA+AGG - tig0025867:23006-23025 Msa1425530:three_prime_UTR 25.0%
! AATCAAAATGCTTCAAGATA+TGG + tig0025867:23957-23976 None:intergenic 25.0%
!! ATTTCTAAGAGTTTATAGCA+AGG + tig0025867:24127-24146 None:intergenic 25.0%
!! ATATAAATGCTATTTGGCAT+TGG - tig0025867:24602-24621 Msa1425530:CDS 25.0%
! CAAAACAAGTTAGAACATTT+CGG + tig0025867:24832-24851 None:intergenic 25.0%
!!! AATGTTCTAACTTGTTTTGA+AGG - tig0025867:24833-24852 Msa1425530:CDS 25.0%
!!! CTTGTTTTGAAGGTTTTTAT+TGG - tig0025867:24843-24862 Msa1425530:CDS 25.0%
! TAAATCTATGCATACTAAGT+TGG - tig0025867:25373-25392 Msa1425530:exon 25.0%
TGGTGATTATGGATAATCTT+TGG + tig0025867:22537-22556 None:intergenic 30.0%
! TGATAGTGTTGTATTTGATG+TGG + tig0025867:22571-22590 None:intergenic 30.0%
TCACATTAGCATAAACCAAA+TGG - tig0025867:22650-22669 Msa1425530:three_prime_UTR 30.0%
GTCTTTAATTCAAGTGTGTT+TGG - tig0025867:23564-23583 Msa1425530:CDS 30.0%
GTATAGAGGATAAAAGATAG+TGG - tig0025867:23780-23799 Msa1425530:CDS 30.0%
ACATGGTCAAGTAAGAAAAT+AGG - tig0025867:23909-23928 Msa1425530:intron 30.0%
! ATAAGTGTTGTGATAGAGAA+TGG + tig0025867:24248-24267 None:intergenic 30.0%
AAAGAAAGCAACACTTCAAT+TGG - tig0025867:24391-24410 Msa1425530:intron 30.0%
TAAAGGACCACTTAACAATT+GGG - tig0025867:24484-24503 Msa1425530:intron 30.0%
TGAGTTAATCAAGAACTAAG+AGG + tig0025867:24561-24580 None:intergenic 30.0%
GTTCTTGATTAACTCATGTA+TGG - tig0025867:24565-24584 Msa1425530:intron 30.0%
GTGAAATTCCTAATGTGAAT+TGG - tig0025867:24659-24678 Msa1425530:CDS 30.0%
TTGACTAAGCATGAAATTTG+AGG - tig0025867:24733-24752 Msa1425530:CDS 30.0%
TGACTAAGCATGAAATTTGA+GGG - tig0025867:24734-24753 Msa1425530:CDS 30.0%
GAAATCAATAGGATGAGAAA+AGG - tig0025867:24915-24934 Msa1425530:CDS 30.0%
!! AACTTGTTACCCAAACAAAA+TGG - tig0025867:25020-25039 Msa1425530:intron 30.0%
! ACTTGTTACCCAAACAAAAT+GGG - tig0025867:25021-25040 Msa1425530:intron 30.0%
!! GAATAACTTGTTAGAGGATT+TGG - tig0025867:25280-25299 Msa1425530:CDS 30.0%
! TGTTCATTTTGGATAACAAG+TGG - tig0025867:25402-25421 Msa1425530:five_prime_UTR 30.0%
! GTTCATTTTGGATAACAAGT+GGG - tig0025867:25403-25422 Msa1425530:five_prime_UTR 30.0%
! TTCATTTTGGATAACAAGTG+GGG - tig0025867:25404-25423 Msa1425530:five_prime_UTR 30.0%
! TGGTGATGAATTGGTGATTA+TGG + tig0025867:22548-22567 None:intergenic 35.0%
TAGTGTTGTATTTGATGTGG+TGG + tig0025867:22568-22587 None:intergenic 35.0%
! CTTGATCTTGATGGTTTCTT+TGG + tig0025867:22795-22814 None:intergenic 35.0%
GATTACGGATTTGATCCATT+TGG - tig0025867:23172-23191 Msa1425530:three_prime_UTR 35.0%
! GGATTTGATCCATTTGGTTT+GGG - tig0025867:23178-23197 Msa1425530:three_prime_UTR 35.0%
!! GATTTGATCCATTTGGTTTG+GGG - tig0025867:23179-23198 Msa1425530:three_prime_UTR 35.0%
AGTCCAAATACTTCACTGTA+AGG + tig0025867:23328-23347 None:intergenic 35.0%
TATTTGGACTTCAGAGGTTT+AGG - tig0025867:23338-23357 Msa1425530:three_prime_UTR 35.0%
ATTTGGACTTCAGAGGTTTA+GGG - tig0025867:23339-23358 Msa1425530:three_prime_UTR 35.0%
!! TGAGTTGATTCATGGTAGAT+GGG - tig0025867:23366-23385 Msa1425530:three_prime_UTR 35.0%
TAATTCAAGTGTGTTTGGCT+CGG - tig0025867:23569-23588 Msa1425530:CDS 35.0%
GTAGAAGGATCAACATATCT+TGG - tig0025867:24212-24231 Msa1425530:intron 35.0%
TCTCTATCACAACACTTATC+TGG - tig0025867:24249-24268 Msa1425530:intron 35.0%
! TGGATCGAAGTTCTAGTAAT+TGG - tig0025867:24269-24288 Msa1425530:intron 35.0%
!!! GAAGTGTTGCTTTCTTTTCT+GGG + tig0025867:24388-24407 None:intergenic 35.0%
!!! TGAAGTGTTGCTTTCTTTTC+TGG + tig0025867:24389-24408 None:intergenic 35.0%
GTAAAGGACCACTTAACAAT+TGG - tig0025867:24483-24502 Msa1425530:intron 35.0%
!! GGAAGAAAAGGTATCAATGA+TGG + tig0025867:24540-24559 None:intergenic 35.0%
CAAGAACTAAGAGGAAGAAA+AGG + tig0025867:24552-24571 None:intergenic 35.0%
!! GCATTGGTTTGTAAACATTG+TGG - tig0025867:24618-24637 Msa1425530:CDS 35.0%
TAACTGCTCCAATTCACATT+AGG + tig0025867:24670-24689 None:intergenic 35.0%
AATGTGAATTGGAGCAGTTA+AGG - tig0025867:24670-24689 Msa1425530:CDS 35.0%
TCATAGGGTGGAAAAAGTAA+TGG + tig0025867:24777-24796 None:intergenic 35.0%
ATCAATAGGATGAGAAAAGG+AGG - tig0025867:24918-24937 Msa1425530:CDS 35.0%
!! GTTTAAGGCCCATTTTGTTT+GGG + tig0025867:25032-25051 None:intergenic 35.0%
!! TGTTTAAGGCCCATTTTGTT+TGG + tig0025867:25033-25052 None:intergenic 35.0%
CAAAATGGGCCTTAAACATT+AGG - tig0025867:25035-25054 Msa1425530:intron 35.0%
ACACTTCGACCTAATGTTTA+AGG + tig0025867:25047-25066 None:intergenic 35.0%
TAAACATTAGGTCGAAGTGT+TGG - tig0025867:25047-25066 Msa1425530:intron 35.0%
! AAACATTAGGTCGAAGTGTT+GGG - tig0025867:25048-25067 Msa1425530:intron 35.0%
AAACACCTTAAAAGAAGCGA+CGG - tig0025867:25169-25188 Msa1425530:intron 35.0%
!!! GAATTTTTTCGTTTGGGAAG+AGG - tig0025867:25250-25269 Msa1425530:CDS 35.0%
!! TAACTTGTTAGAGGATTTGG+AGG - tig0025867:25283-25302 Msa1425530:CDS 35.0%
!! AACTTGTTAGAGGATTTGGA+GGG - tig0025867:25284-25303 Msa1425530:CDS 35.0%
TTTGGAAGTTAGAAGAGGAT+GGG - tig0025867:25339-25358 Msa1425530:CDS 35.0%
! TCTATGCATACTAAGTTGGA+AGG - tig0025867:25377-25396 Msa1425530:exon 35.0%
!! GTTGGAAGGTATGTTCATTT+TGG - tig0025867:25391-25410 Msa1425530:five_prime_UTR 35.0%
! TTGATGTGGTGGTGATGAAT+TGG + tig0025867:22557-22576 None:intergenic 40.0%
GGTTTATGCTAATGTGAGCA+GGG + tig0025867:22647-22666 None:intergenic 40.0%
TGGTTTATGCTAATGTGAGC+AGG + tig0025867:22648-22667 None:intergenic 40.0%
AAGTTCAATCCAATTCGGGT+AGG - tig0025867:22730-22749 Msa1425530:three_prime_UTR 40.0%
TCTTCTTTCCAAAGCCGAAT+CGG + tig0025867:22764-22783 None:intergenic 40.0%
! TCTTGATGGTTTCTTTGGTG+CGG + tig0025867:22790-22809 None:intergenic 40.0%
CGGATTTGATCCATTTGGTT+TGG - tig0025867:23177-23196 Msa1425530:three_prime_UTR 40.0%
TCAAATTGCAAATACTCCGC+TGG + tig0025867:23208-23227 None:intergenic 40.0%
AATTTGGCGAAGAATCTTGC+CGG - tig0025867:23247-23266 Msa1425530:three_prime_UTR 40.0%
ACTTCACTGTAAGGCTGAAA+TGG + tig0025867:23319-23338 None:intergenic 40.0%
! CAGCCTTACAGTGAAGTATT+TGG - tig0025867:23322-23341 Msa1425530:three_prime_UTR 40.0%
! GTGAAGTATTTGGACTTCAG+AGG - tig0025867:23332-23351 Msa1425530:three_prime_UTR 40.0%
!! AGGGAATGTGAGTTGATTCA+TGG - tig0025867:23358-23377 Msa1425530:three_prime_UTR 40.0%
!! GTGAGTTGATTCATGGTAGA+TGG - tig0025867:23365-23384 Msa1425530:three_prime_UTR 40.0%
! TTGGTGCTCTTACTGTTGAA+TGG - tig0025867:23404-23423 Msa1425530:three_prime_UTR 40.0%
CTTACTGTTGAATGGCTTAC+TGG - tig0025867:23412-23431 Msa1425530:three_prime_UTR 40.0%
!! AATGGCTTACTGGTGTTACA+TGG - tig0025867:23422-23441 Msa1425530:three_prime_UTR 40.0%
!! TTTCAGGTTGAGCTAGTAGA+AGG - tig0025867:24197-24216 Msa1425530:intron 40.0%
GTTGTGATAGAGAATGGAAG+TGG + tig0025867:24242-24261 None:intergenic 40.0%
! GGTCAAAGAATTTTCCACCT+GGG + tig0025867:24346-24365 None:intergenic 40.0%
GGAAAATTCTTTGACCCACT+TGG - tig0025867:24350-24369 Msa1425530:intron 40.0%
TGAGTAGCCCAATTGTTAAG+TGG + tig0025867:24494-24513 None:intergenic 40.0%
! TCAATGATGGTAGTGTGAAG+AGG + tig0025867:24527-24546 None:intergenic 40.0%
CATGAAATTTGAGGGCAATG+AGG - tig0025867:24742-24761 Msa1425530:CDS 40.0%
!! AACATTAGGTCGAAGTGTTG+GGG - tig0025867:25049-25068 Msa1425530:intron 40.0%
! CTTTGGAAGTTAGAAGAGGA+TGG - tig0025867:25338-25357 Msa1425530:CDS 40.0%
TTTGGATAACAAGTGGGGTA+CGG - tig0025867:25409-25428 Msa1425530:five_prime_UTR 40.0%
TTATGCTAATGTGAGCAGGG+TGG + tig0025867:22644-22663 None:intergenic 45.0%
GCAGCCACTTCATCATTCAT+CGG - tig0025867:22693-22712 Msa1425530:three_prime_UTR 45.0%
AATTGGATTGAACTTCGCGG+AGG + tig0025867:22725-22744 None:intergenic 45.0%
CCGAATTGGATTGAACTTCG+CGG + tig0025867:22728-22747 None:intergenic 45.0%
CCGCGAAGTTCAATCCAATT+CGG - tig0025867:22725-22744 Msa1425530:three_prime_UTR 45.0%
CGCGAAGTTCAATCCAATTC+GGG - tig0025867:22726-22745 Msa1425530:three_prime_UTR 45.0%
CTTCTTTCCAAAGCCGAATC+GGG + tig0025867:22763-22782 None:intergenic 45.0%
GTACTGGGACTTGATCTTGA+TGG + tig0025867:22804-22823 None:intergenic 45.0%
!! CAGGTACTGATCGTCCATTA+TGG - tig0025867:23101-23120 Msa1425530:three_prime_UTR 45.0%
GGAAGTTTGGTCGGAGATTA+CGG - tig0025867:23157-23176 Msa1425530:three_prime_UTR 45.0%
!! ATTTGGTTTGGGGAAACCAG+CGG - tig0025867:23189-23208 Msa1425530:three_prime_UTR 45.0%
CGACTCGCTTGATCAGAATT+TGG - tig0025867:23231-23250 Msa1425530:three_prime_UTR 45.0%
AATCTTGCCGGAGATGTGAT+CGG - tig0025867:23259-23278 Msa1425530:three_prime_UTR 45.0%
AGATGTGATCGGAACTAGGA+CGG - tig0025867:23270-23289 Msa1425530:three_prime_UTR 45.0%
TACATGGCAAGATGCTGGAA+AGG - tig0025867:23438-23457 Msa1425530:three_prime_UTR 45.0%
CATCAAGCTCAGCATTTCTC+TGG + tig0025867:24307-24326 None:intergenic 45.0%
GCAGAAAAGAGACTATACCC+AGG - tig0025867:24326-24345 Msa1425530:intron 45.0%
GAAAAGAGACTATACCCAGG+TGG - tig0025867:24329-24348 Msa1425530:intron 45.0%
! GGGTCAAAGAATTTTCCACC+TGG + tig0025867:24347-24366 None:intergenic 45.0%
CAAACATGCTCGTCTTGCTA+TGG - tig0025867:24421-24440 Msa1425530:intron 45.0%
! CTTGCTATGGTTGCTTTCCT+TGG - tig0025867:24434-24453 Msa1425530:intron 45.0%
CAGCTTGAACAGCAAAACCA+AGG + tig0025867:24454-24473 None:intergenic 45.0%
TGAGGGCAATGAGGAACATT+AGG - tig0025867:24751-24770 Msa1425530:CDS 45.0%
! AGTTTCTCGCGCGTCTTATT+GGG - tig0025867:24796-24815 Msa1425530:CDS 45.0%
AGGATGAGAAAAGGAGGAGT+CGG - tig0025867:24924-24943 Msa1425530:CDS 45.0%
!!! GCGGCGGAATTTTTTCGTTT+GGG - tig0025867:25244-25263 Msa1425530:CDS 45.0%
AGGATTTGGAGGGTCATGTT+GGG - tig0025867:25294-25313 Msa1425530:CDS 45.0%
!! GTGGCTTTGGAAGTTAGAAG+AGG - tig0025867:25334-25353 Msa1425530:CDS 45.0%
! AGAAGAGGATGGGAGGTTTT+CGG - tig0025867:25349-25368 Msa1425530:CDS 45.0%
AAGCGGTGTTTAGACAAGTG+TGG - tig0025867:25438-25457 Msa1425530:five_prime_UTR 45.0%
TAGACAAGTGTGGAAGAGTC+CGG - tig0025867:25448-25467 Msa1425530:five_prime_UTR 45.0%
! CGTGGAAGCTTTTACTTGAC+CGG - tig0025867:25495-25514 Msa1425530:five_prime_UTR 45.0%
GAATGATGAAGTGGCTGCAG+CGG + tig0025867:22691-22710 None:intergenic 50.0%
!! GGTGCCGATGAATGATGAAG+TGG + tig0025867:22700-22719 None:intergenic 50.0%
GATCGTCCATTATGGTACCC+CGG - tig0025867:23109-23128 Msa1425530:three_prime_UTR 50.0%
! TTTGCTCCGGGGTACCATAA+TGG + tig0025867:23118-23137 None:intergenic 50.0%
AAACTTCCGTCTAGCCACTC+CGG + tig0025867:23145-23164 None:intergenic 50.0%
TGGCTAGACGGAAGTTTGGT+CGG - tig0025867:23148-23167 Msa1425530:three_prime_UTR 50.0%
! GCTGGTTTCCCCAAACCAAA+TGG + tig0025867:23190-23209 None:intergenic 50.0%
CCTAGTTCCGATCACATCTC+CGG + tig0025867:23269-23288 None:intergenic 50.0%
! TGGGCTATGCTTGCTACTCT+TGG - tig0025867:23385-23404 Msa1425530:three_prime_UTR 50.0%
! GGTGTTACATGGCAAGATGC+TGG - tig0025867:23433-23452 Msa1425530:three_prime_UTR 50.0%
GGTCAGATGCTAAGCCAAGT+GGG + tig0025867:24367-24386 None:intergenic 50.0%
CAAGCTGCTGCTACTGGTAA+AGG - tig0025867:24467-24486 Msa1425530:intron 50.0%
AGACGCGCGAGAAACTCATA+GGG + tig0025867:24792-24811 None:intergenic 50.0%
AAGACGCGCGAGAAACTCAT+AGG + tig0025867:24793-24812 None:intergenic 50.0%
GAGTTTCTCGCGCGTCTTAT+TGG - tig0025867:24795-24814 Msa1425530:CDS 50.0%
AGGGCGCGCAAGAAATCAAT+AGG - tig0025867:24904-24923 Msa1425530:CDS 50.0%
CACCTTAAAAGAAGCGACGG+TGG - tig0025867:25172-25191 Msa1425530:intron 50.0%
CTTAAAAGAAGCGACGGTGG+CGG - tig0025867:25175-25194 Msa1425530:intron 50.0%
GTTTGGGTGAATGGCGAGAT+GGG - tig0025867:25200-25219 Msa1425530:intron 50.0%
GAAGGGAGTGGAGGTTTATG+TGG - tig0025867:25222-25241 Msa1425530:intron 50.0%
!!! GGCGGCGGAATTTTTTCGTT+TGG - tig0025867:25243-25262 Msa1425530:CDS 50.0%
GAGGATTTGGAGGGTCATGT+TGG - tig0025867:25293-25312 Msa1425530:CDS 50.0%
TCATGTTGGGTCTCATGAGG+AGG - tig0025867:25307-25326 Msa1425530:CDS 50.0%
ATGAGGAGGATGAGTGGCTT+TGG - tig0025867:25321-25340 Msa1425530:CDS 50.0%
GGAAGTTAGAAGAGGATGGG+AGG - tig0025867:25342-25361 Msa1425530:CDS 50.0%
TGGATTGAACTTCGCGGAGG+CGG + tig0025867:22722-22741 None:intergenic 55.0%
GGCTTGGATCCTACCCGAAT+TGG + tig0025867:22742-22761 None:intergenic 55.0%
GGTAGGATCCAAGCCCGATT+CGG - tig0025867:22747-22766 Msa1425530:three_prime_UTR 55.0%
!! ATCCAAGCCCGATTCGGCTT+TGG - tig0025867:22753-22772 Msa1425530:three_prime_UTR 55.0%
TTCCAAAGCCGAATCGGGCT+TGG + tig0025867:22758-22777 None:intergenic 55.0%
AAGTCACCGGAGTGGCTAGA+CGG - tig0025867:23136-23155 Msa1425530:three_prime_UTR 55.0%
GGAGTGGCTAGACGGAAGTT+TGG - tig0025867:23144-23163 Msa1425530:three_prime_UTR 55.0%
CCGGAGATGTGATCGGAACT+AGG - tig0025867:23266-23285 Msa1425530:three_prime_UTR 55.0%
GGGTCAGATGCTAAGCCAAG+TGG + tig0025867:24368-24387 None:intergenic 55.0%
GCTGTTCAAGCTGCTGCTAC+TGG - tig0025867:24461-24480 Msa1425530:intron 55.0%
! CGCCACCGTCGCTTCTTTTA+AGG + tig0025867:25177-25196 None:intergenic 55.0%
GGTTTGGGTGAATGGCGAGA+TGG - tig0025867:25199-25218 Msa1425530:intron 55.0%
GGTGAATGGCGAGATGGGAA+GGG - tig0025867:25205-25224 Msa1425530:intron 55.0%
GGGAGTGGAGGTTTATGTGG+CGG - tig0025867:25225-25244 Msa1425530:intron 55.0%
AGTGGAGGTTTATGTGGCGG+CGG - tig0025867:25228-25247 Msa1425530:intron 55.0%
GGGTCATGTTGGGTCTCATG+AGG - tig0025867:25304-25323 Msa1425530:CDS 55.0%
GGTCTCATGAGGAGGATGAG+TGG - tig0025867:25315-25334 Msa1425530:CDS 55.0%
GGATAACAAGTGGGGTACGG+AGG - tig0025867:25412-25431 Msa1425530:five_prime_UTR 55.0%
GATAACGCCACTGCCTTCGA+GGG + tig0025867:25476-25495 None:intergenic 55.0%
TGATGAAGTGGCTGCAGCGG+TGG + tig0025867:22688-22707 None:intergenic 60.0%
GGATTGAACTTCGCGGAGGC+GGG + tig0025867:22721-22740 None:intergenic 60.0%
GTACCCCGGAGCAAAGTCAC+CGG - tig0025867:23123-23142 Msa1425530:three_prime_UTR 60.0%
ACTCCGGTGACTTTGCTCCG+GGG + tig0025867:23129-23148 None:intergenic 60.0%
CACTCCGGTGACTTTGCTCC+GGG + tig0025867:23130-23149 None:intergenic 60.0%
CCACTCCGGTGACTTTGCTC+CGG + tig0025867:23131-23150 None:intergenic 60.0%
CGCGCGAGAAACTCATAGGG+TGG + tig0025867:24789-24808 None:intergenic 60.0%
! AAAAGAAGCGACGGTGGCGG+AGG - tig0025867:25178-25197 Msa1425530:intron 60.0%
! GTGGCGGAGGTTTGGGTGAA+TGG - tig0025867:25191-25210 Msa1425530:intron 60.0%
GGGTGAATGGCGAGATGGGA+AGG - tig0025867:25204-25223 Msa1425530:intron 60.0%
ATGGCGAGATGGGAAGGGAG+TGG - tig0025867:25210-25229 Msa1425530:intron 60.0%
GTGGGGTACGGAGGAGAAAG+CGG - tig0025867:25421-25440 Msa1425530:five_prime_UTR 60.0%
CGATAACGCCACTGCCTTCG+AGG + tig0025867:25477-25496 None:intergenic 60.0%
CGAAGGCAGTGGCGTTATCG+TGG - tig0025867:25477-25496 Msa1425530:five_prime_UTR 60.0%
TGAAGTGGCTGCAGCGGTGG+CGG + tig0025867:22685-22704 None:intergenic 65.0%
CCGGAGCAAAGTCACCGGAG+TGG - tig0025867:23128-23147 Msa1425530:three_prime_UTR 65.0%
!! AAGCGACGGTGGCGGAGGTT+TGG - tig0025867:25183-25202 Msa1425530:intron 65.0%
!! AGCGACGGTGGCGGAGGTTT+GGG - tig0025867:25184-25203 Msa1425530:intron 65.0%
GCGAGATGGGAAGGGAGTGG+AGG - tig0025867:25213-25232 Msa1425530:intron 65.0%
GAAGAGTCCGGCACCCTCGA+AGG - tig0025867:25460-25479 Msa1425530:five_prime_UTR 65.0%
TGGCGGTTGCGGCGGCCATT+TGG + tig0025867:22668-22687 None:intergenic 70.0%
TCCGGCACCCTCGAAGGCAG+TGG - tig0025867:25466-25485 Msa1425530:five_prime_UTR 70.0%
! GCCACTGCCTTCGAGGGTGC+CGG + tig0025867:25470-25489 None:intergenic 70.0%
! TGCAGCGGTGGCGGTTGCGG+CGG + tig0025867:22676-22695 None:intergenic 75.0%
! GGCTGCAGCGGTGGCGGTTG+CGG + tig0025867:22679-22698 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0025867 gene 22523 25527 22523 ID=Msa1425530;Name=Msa1425530
tig0025867 mRNA 22523 25527 22523 ID=Msa1425530-mRNA-1;Parent=Msa1425530;Name=Msa1425530-mRNA-1;_AED=0.06;_eAED=0.06;_QI=146|1|1|1|1|1|3|962|289
tig0025867 exon 22523 23847 22523 ID=Msa1425530-mRNA-1:exon:4367;Parent=Msa1425530-mRNA-1
tig0025867 exon 24591 24946 24591 ID=Msa1425530-mRNA-1:exon:4366;Parent=Msa1425530-mRNA-1
tig0025867 exon 25231 25527 25231 ID=Msa1425530-mRNA-1:exon:4365;Parent=Msa1425530-mRNA-1
tig0025867 five_prime_UTR 25382 25527 25382 ID=Msa1425530-mRNA-1:five_prime_utr;Parent=Msa1425530-mRNA-1
tig0025867 CDS 25231 25381 25231 ID=Msa1425530-mRNA-1:cds;Parent=Msa1425530-mRNA-1
tig0025867 CDS 24591 24946 24591 ID=Msa1425530-mRNA-1:cds;Parent=Msa1425530-mRNA-1
tig0025867 CDS 23485 23847 23485 ID=Msa1425530-mRNA-1:cds;Parent=Msa1425530-mRNA-1
tig0025867 three_prime_UTR 22523 23484 22523 ID=Msa1425530-mRNA-1:three_prime_utr;Parent=Msa1425530-mRNA-1
Gene Sequence

>Msa1425530

ATGGCCGCCGCAACCGCCACCGCTGCAGCCACTTCATCATTCATCGGCACCCGCCTCCGCGAAGTTCAATCCAATTCGGGTAGGATCCAAGCCCGATTCGGCTTTGGAAAGAAGAAAGCCGCACCAAAGAAACCATCAAGATCAAGTCCCAGTACTGATCGTCCATTATGGTACCCCGGAGCAAAGTCACCGGAGTGGCTAGACGGAAGTTTGGTCGGAGATTACGGATTTGATCCATTTGGTTTGGGGAAACCAGCGGAGTATTTGCAATTTGATCTCGACTCGCTTGATCAGAATTTGGCGAAGAATCTTGCCGGAGATGTGATCGGAACTAGGACGGAGTTTGCTGATGTGAAATCAACTCCATTTCAGCCTTACAGTGAAGTATTTGGACTTCAGAGGTTTAGGGAATGTGAGTTGATTCATGGTAGATGGGCTATGCTTGCTACTCTTGGTGCTCTTACTGTTGAATGGCTTACTGGTGTTACATGGCAAGATGCTGGAAAGGTTGAGCTAGTAGAAGGATCAACATATCTTGGACAACCACTTCCATTCTCTATCACAACACTTATCTGGATCGAAGTTCTAGTAATTGGCTACATTGAATTCCAGAGAAATGCTGAGCTTGATGCAGAAAAGAGACTATACCCAGGTGGAAAATTCTTTGACCCACTTGGCTTAGCATCTGACCCAGAAAAGAAAGCAACACTTCAATTGGCAGAAATCAAACATGCTCGTCTTGCTATGGTTGCTTTCCTTGGTTTTGCTGTTCAAGCTGCTGCTACTGGTAAAGGACCACTTAACAATTGGGCTACTCATTTGAGTGATCCTCTTCACACTACCATCATTGATACCTTTTCTTCCTCTTAG
Protein sequence

>Msa1425530

MAAATATAAATSSFIGTRLREVQSNSGRIQARFGFGKKKAAPKKPSRSSPSTDRPLWYPGAKSPEWLDGSLVGDYGFDPFGLGKPAEYLQFDLDSLDQNLAKNLAGDVIGTRTEFADVKSTPFQPYSEVFGLQRFRECELIHGRWAMLATLGALTVEWLTGVTWQDAGKVELVEGSTYLGQPLPFSITTLIWIEVLVIGYIEFQRNAELDAEKRLYPGGKFFDPLGLASDPEKKATLQLAEIKHARLAMVAFLGFAVQAAATGKGPLNNWATHLSDPLHTTIIDTFSSS*