AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0190710


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0190710 MtrunA17_Chr2g0280471 96.988 166 5 0 1 166 1 166 1.74e-118 331
Msa0190710 MtrunA17_Chr2g0280451 86.747 166 22 0 1 166 1 166 6.79e-105 297
Msa0190710 MtrunA17_Chr7g0249341 56.627 166 53 1 1 166 1 147 4.82e-64 193
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0190710 AT5G56260.1 75.301 166 41 0 1 166 1 166 1.61e-90 261
Msa0190710 AT5G56260.2 75.301 166 41 0 1 166 1 166 1.61e-90 261
Msa0190710 AT5G56260.5 75.301 166 41 0 1 166 1 166 1.61e-90 261
Msa0190710 AT5G56260.4 75.301 166 41 0 1 166 33 198 1.07e-89 260
Msa0190710 AT5G56260.3 75.301 166 41 0 1 166 33 198 1.07e-89 260
Msa0190710 AT3G02770.1 65.060 166 58 0 1 166 1 166 9.65e-78 229
Msa0190710 AT5G16450.2 65.060 166 58 0 1 166 1 166 1.48e-77 228
Msa0190710 AT5G16450.1 65.060 166 58 0 1 166 1 166 1.48e-77 228

Find 37 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCTTGTTGGAGGGAATT+TGG 0.199853 2_1:+6240120 Msa0190710:CDS
TGGCCAGTCTCGGGCATTCT+CGG 0.281731 2_1:+6239985 Msa0190710:CDS
GCACAAAACATGGGGTGGTC+TGG 0.304278 2_1:+6240151 Msa0190710:CDS
GGCCAGTCTCGGGCATTCTC+GGG 0.351904 2_1:+6239986 Msa0190710:CDS
TGGTCTGGGATTGTTGTGAA+TGG 0.381293 2_1:+6240166 Msa0190710:CDS
AAAAGGAGAAGGAAGAGTTT+TGG 0.387441 2_1:+6240069 Msa0190710:CDS
CACAAAACATGGGGTGGTCT+GGG 0.391877 2_1:+6240152 Msa0190710:CDS
CCCGAGACTGGCCATACATC+TGG 0.415145 2_1:-6239976 None:intergenic
GCCCCGAGAATGCCCGAGAC+TGG 0.420007 2_1:-6239988 None:intergenic
TCAAGTTGGTGTTAGAGCTT+TGG 0.422544 2_1:+6240222 Msa0190710:CDS
ACCTCTAGTACTATTTCAAA+TGG 0.429193 2_1:-6240383 None:intergenic
AACGCAACACATTTAACAAA+TGG 0.449158 2_1:+6239926 Msa0190710:CDS
TCCAGATGTATGGCCAGTCT+CGG 0.458278 2_1:+6239975 Msa0190710:CDS
GGTGCAGTTGGCACAAAACA+TGG 0.464361 2_1:+6240141 Msa0190710:CDS
CATCCAGTGTTCCAGATGTA+TGG 0.472302 2_1:+6239965 Msa0190710:CDS
TCAAATGGACAACTCAAACT+TGG 0.482072 2_1:-6240368 None:intergenic
TGGAGGGAATTTGGTGCAGT+TGG 0.484334 2_1:+6240129 Msa0190710:CDS
AGAGAACTTCTTGAAACAAA+AGG 0.491488 2_1:+6240052 Msa0190710:CDS
GAGATTAATTTGTGTCAAGT+TGG 0.491931 2_1:+6240208 Msa0190710:CDS
CATGTTGCTGTTTATGTTGG+AGG 0.502059 2_1:+6240289 Msa0190710:CDS
AAACATGTTGCTGTTTATGT+TGG 0.508506 2_1:+6240286 Msa0190710:CDS
TGGCCATACATCTGGAACAC+TGG 0.512769 2_1:-6239968 None:intergenic
CCAGATGTATGGCCAGTCTC+GGG 0.532810 2_1:+6239976 Msa0190710:CDS
GTGTCACATATCTCTGCTGT+TGG 0.545822 2_1:-6239902 None:intergenic
GCCAGTCTCGGGCATTCTCG+GGG 0.553955 2_1:+6239987 Msa0190710:CDS
AGCATGAGGTGTGCTCTTGT+TGG 0.557997 2_1:+6240109 Msa0190710:CDS
ATGAGGTGTGCTCTTGTTGG+AGG 0.569564 2_1:+6240112 Msa0190710:CDS
CTTCTTGAAACAAAAGGAGA+AGG 0.585218 2_1:+6240058 Msa0190710:CDS
TGGCTCTATGCAGATAATGA+TGG 0.590987 2_1:+6240334 Msa0190710:CDS
ATTTAACAAATGGTGACTTG+AGG 0.616446 2_1:+6239936 Msa0190710:CDS
AGTGTTTGAAGACAATGTGT+TGG 0.617013 2_1:+6240027 Msa0190710:CDS
GTGCAGTTGGCACAAAACAT+GGG 0.623381 2_1:+6240142 Msa0190710:CDS
GAATGGTTGTATTAGAGATG+TGG 0.623790 2_1:+6240183 Msa0190710:CDS
TGAGGTGTGCTCTTGTTGGA+GGG 0.651891 2_1:+6240113 Msa0190710:CDS
AGTTGGCACAAAACATGGGG+TGG 0.672135 2_1:+6240146 Msa0190710:CDS
TGCAGTTGGCACAAAACATG+GGG 0.672279 2_1:+6240143 Msa0190710:CDS
TTGATGGAGGAGGAAGCATG+AGG 0.673507 2_1:+6240095 Msa0190710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGTCTTCAAACACTTTTA+CGG - chr2_1:6240023-6240042 None:intergenic 25.0%
! CCACTTAAATCCAATAAAAA+CGG + chr2_1:6240253-6240272 Msa0190710:CDS 25.0%
AACGCAACACATTTAACAAA+TGG + chr2_1:6239926-6239945 Msa0190710:CDS 30.0%
ATTTAACAAATGGTGACTTG+AGG + chr2_1:6239936-6239955 Msa0190710:CDS 30.0%
AGAGAACTTCTTGAAACAAA+AGG + chr2_1:6240052-6240071 Msa0190710:CDS 30.0%
!! GAGATTAATTTGTGTCAAGT+TGG + chr2_1:6240208-6240227 Msa0190710:CDS 30.0%
!! CCGTTTTTATTGGATTTAAG+TGG - chr2_1:6240256-6240275 None:intergenic 30.0%
AAATCCAATAAAAACGGTAC+TGG + chr2_1:6240259-6240278 Msa0190710:CDS 30.0%
!! AAACATGTTGCTGTTTATGT+TGG + chr2_1:6240286-6240305 Msa0190710:CDS 30.0%
! AACACTTTTACGGTAACAAC+AGG - chr2_1:6240013-6240032 None:intergenic 35.0%
AGTGTTTGAAGACAATGTGT+TGG + chr2_1:6240027-6240046 Msa0190710:CDS 35.0%
CTTCTTGAAACAAAAGGAGA+AGG + chr2_1:6240058-6240077 Msa0190710:CDS 35.0%
! AAAAGGAGAAGGAAGAGTTT+TGG + chr2_1:6240069-6240088 Msa0190710:CDS 35.0%
!!! GGAAGAGTTTTGGTTATTGA+TGG + chr2_1:6240079-6240098 Msa0190710:CDS 35.0%
!!! AGAGTTTTGGTTATTGATGG+AGG + chr2_1:6240082-6240101 Msa0190710:CDS 35.0%
GAATGGTTGTATTAGAGATG+TGG + chr2_1:6240183-6240202 Msa0190710:CDS 35.0%
!! TTCACCAGTACCGTTTTTAT+TGG - chr2_1:6240266-6240285 None:intergenic 35.0%
!! CTTTTATTCGTGATGGAGAA+TGG + chr2_1:6240314-6240333 Msa0190710:CDS 35.0%
TCAAATGGACAACTCAAACT+TGG - chr2_1:6240371-6240390 None:intergenic 35.0%
!!! GTTTTGGTTATTGATGGAGG+AGG + chr2_1:6240085-6240104 Msa0190710:CDS 40.0%
!! TCAAGTTGGTGTTAGAGCTT+TGG + chr2_1:6240222-6240241 Msa0190710:CDS 40.0%
!! CATGTTGCTGTTTATGTTGG+AGG + chr2_1:6240289-6240308 Msa0190710:CDS 40.0%
! GGAGGAACTTTTATTCGTGA+TGG + chr2_1:6240307-6240326 Msa0190710:CDS 40.0%
! TGGCTCTATGCAGATAATGA+TGG + chr2_1:6240334-6240353 Msa0190710:CDS 40.0%
GTGTCACATATCTCTGCTGT+TGG - chr2_1:6239905-6239924 None:intergenic 45.0%
CATCCAGTGTTCCAGATGTA+TGG + chr2_1:6239965-6239984 Msa0190710:CDS 45.0%
TGCTCTTGTTGGAGGGAATT+TGG + chr2_1:6240120-6240139 Msa0190710:CDS 45.0%
GTGCAGTTGGCACAAAACAT+GGG + chr2_1:6240142-6240161 Msa0190710:CDS 45.0%
TGCAGTTGGCACAAAACATG+GGG + chr2_1:6240143-6240162 Msa0190710:CDS 45.0%
! TGGTCTGGGATTGTTGTGAA+TGG + chr2_1:6240166-6240185 Msa0190710:CDS 45.0%
! TGGCCATACATCTGGAACAC+TGG - chr2_1:6239971-6239990 None:intergenic 50.0%
TCCAGATGTATGGCCAGTCT+CGG + chr2_1:6239975-6239994 Msa0190710:CDS 50.0%
! TTGATGGAGGAGGAAGCATG+AGG + chr2_1:6240095-6240114 Msa0190710:CDS 50.0%
AGCATGAGGTGTGCTCTTGT+TGG + chr2_1:6240109-6240128 Msa0190710:CDS 50.0%
ATGAGGTGTGCTCTTGTTGG+AGG + chr2_1:6240112-6240131 Msa0190710:CDS 50.0%
TGAGGTGTGCTCTTGTTGGA+GGG + chr2_1:6240113-6240132 Msa0190710:CDS 50.0%
!! TGGAGGGAATTTGGTGCAGT+TGG + chr2_1:6240129-6240148 Msa0190710:CDS 50.0%
GGTGCAGTTGGCACAAAACA+TGG + chr2_1:6240141-6240160 Msa0190710:CDS 50.0%
AGTTGGCACAAAACATGGGG+TGG + chr2_1:6240146-6240165 Msa0190710:CDS 50.0%
CACAAAACATGGGGTGGTCT+GGG + chr2_1:6240152-6240171 Msa0190710:CDS 50.0%
CCAGATGTATGGCCAGTCTC+GGG + chr2_1:6239976-6239995 Msa0190710:CDS 55.0%
GCACAAAACATGGGGTGGTC+TGG + chr2_1:6240151-6240170 Msa0190710:CDS 55.0%
! CCCGAGACTGGCCATACATC+TGG - chr2_1:6239979-6239998 None:intergenic 60.0%
TGGCCAGTCTCGGGCATTCT+CGG + chr2_1:6239985-6240004 Msa0190710:CDS 60.0%
GGCCAGTCTCGGGCATTCTC+GGG + chr2_1:6239986-6240005 Msa0190710:CDS 65.0%
GCCAGTCTCGGGCATTCTCG+GGG + chr2_1:6239987-6240006 Msa0190710:CDS 65.0%
GCCCCGAGAATGCCCGAGAC+TGG - chr2_1:6239991-6240010 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 6239890 6240390 6239890 ID=Msa0190710;Name=Msa0190710
chr2_1 mRNA 6239890 6240390 6239890 ID=Msa0190710-mRNA-1;Parent=Msa0190710;Name=Msa0190710-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|-1|0|1|-1|1|1|0|166
chr2_1 exon 6239890 6240390 6239890 ID=Msa0190710-mRNA-1:exon:2814;Parent=Msa0190710-mRNA-1
chr2_1 CDS 6239890 6240390 6239890 ID=Msa0190710-mRNA-1:cds;Parent=Msa0190710-mRNA-1
Gene Sequence

>Msa0190710

ATGGATTCTCTACCAACAGCAGAGATATGTGACACAAACGCAACACATTTAACAAATGGTGACTTGAGGATACTTCATCCAGTGTTCCAGATGTATGGCCAGTCTCGGGCATTCTCGGGGCCTGTTGTTACCGTAAAAGTGTTTGAAGACAATGTGTTGGTAAGAGAACTTCTTGAAACAAAAGGAGAAGGAAGAGTTTTGGTTATTGATGGAGGAGGAAGCATGAGGTGTGCTCTTGTTGGAGGGAATTTGGTGCAGTTGGCACAAAACATGGGGTGGTCTGGGATTGTTGTGAATGGTTGTATTAGAGATGTGGATGAGATTAATTTGTGTCAAGTTGGTGTTAGAGCTTTGGCTTCTCATCCACTTAAATCCAATAAAAACGGTACTGGTGAAAAACATGTTGCTGTTTATGTTGGAGGAACTTTTATTCGTGATGGAGAATGGCTCTATGCAGATAATGATGGTATCATTGTATCCAAGTTTGAGTTGTCCATTTGA

Protein sequence

>Msa0190710

MDSLPTAEICDTNATHLTNGDLRILHPVFQMYGQSRAFSGPVVTVKVFEDNVLVRELLETKGEGRVLVIDGGGSMRCALVGGNLVQLAQNMGWSGIVVNGCIRDVDEINLCQVGVRALASHPLKSNKNGTGEKHVAVYVGGTFIRDGEWLYADNDGIIVSKFELSI*