AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0499950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0499950 MtrunA17_Chr3g0097891 84.783 230 6 1 9 238 1 201 2.05e-139 394
Msa0499950 MtrunA17_Chr2g0314801 54.936 233 73 3 9 238 1 204 5.28e-84 254
Msa0499950 MtrunA17_Chr5g0413851 53.648 233 73 3 9 237 8 209 6.62e-82 249
Msa0499950 MtrunA17_Chr4g0045941 45.045 222 92 2 13 234 8 199 2.89e-67 211
Msa0499950 MtrunA17_Chr2g0325481 49.500 200 72 1 39 238 50 220 3.35e-67 211
Msa0499950 MtrunA17_Chr4g0041011 49.500 200 72 1 39 238 47 217 2.06e-66 209
Msa0499950 MtrunA17_Chr2g0283581 44.017 234 94 3 11 237 7 210 4.40e-65 206
Msa0499950 MtrunA17_Chr3g0085071 48.000 200 75 1 39 238 46 216 4.99e-62 198
Msa0499950 MtrunA17_Chr4g0045951 42.017 238 94 3 13 237 8 214 2.10e-61 196
Msa0499950 MtrunA17_Chr2g0314791 47.826 207 71 5 39 237 29 206 2.57e-61 196
Msa0499950 MtrunA17_Chr3g0091031 44.105 229 88 4 12 238 11 201 2.47e-60 193
Msa0499950 MtrunA17_Chr3g0085081 44.105 229 88 4 12 238 11 201 3.37e-60 193
Msa0499950 MtrunA17_Chr1g0209921 43.946 223 90 7 18 235 22 214 6.64e-57 182
Msa0499950 MtrunA17_Chr5g0430371 46.305 203 79 2 36 237 55 228 1.85e-56 184
Msa0499950 MtrunA17_Chr3g0105301 42.791 215 86 3 24 237 35 213 7.70e-52 172
Msa0499950 MtrunA17_Chr5g0437601 34.450 209 100 4 31 231 75 254 1.88e-40 143
Msa0499950 MtrunA17_Chr8g0360521 32.524 206 102 4 36 233 68 244 2.90e-39 140
Msa0499950 MtrunA17_Chr3g0113671 34.597 211 101 4 32 234 99 280 1.16e-38 139
Msa0499950 MtrunA17_Chr3g0114101 35.821 201 92 4 39 231 89 260 2.11e-37 135
Msa0499950 MtrunA17_Chr1g0210771 35.176 199 97 4 39 235 59 227 2.40e-37 134
Msa0499950 MtrunA17_Chr1g0171171 31.707 246 121 5 7 235 6 221 2.42e-37 134
Msa0499950 MtrunA17_Chr7g0269991 35.922 206 91 5 39 232 137 313 3.54e-37 135
Msa0499950 MtrunA17_Chr2g0287901 34.300 207 93 4 38 230 96 273 1.99e-35 130
Msa0499950 MtrunA17_Chr3g0126021 33.906 233 105 6 20 233 110 312 2.14e-35 130
Msa0499950 MtrunA17_Chr4g0006991 32.524 206 102 4 34 231 81 257 4.00e-35 129
Msa0499950 MtrunA17_Chr1g0213281 34.762 210 95 5 36 232 172 352 1.34e-34 129
Msa0499950 MtrunA17_Chr7g0259711 33.495 206 94 4 39 230 91 267 3.80e-34 127
Msa0499950 MtrunA17_Chr4g0071391 32.367 207 98 5 39 232 109 286 1.17e-32 123
Msa0499950 MtrunA17_Chr7g0242701 30.952 210 108 4 34 235 81 261 2.12e-32 122
Msa0499950 MtrunA17_Chr1g0175671 34.300 207 94 5 39 232 170 347 6.55e-32 122
Msa0499950 MtrunA17_Chr5g0437291 32.524 206 99 5 39 233 165 341 2.35e-31 120
Msa0499950 MtrunA17_Chr4g0005491 29.918 244 123 7 6 230 20 234 3.75e-31 118
Msa0499950 MtrunA17_Chr7g0258851 33.816 207 95 6 39 232 248 425 6.92e-31 119
Msa0499950 MtrunA17_Chr7g0243301 32.212 208 102 5 33 230 58 236 7.27e-31 117
Msa0499950 MtrunA17_Chr1g0178081 30.802 237 115 6 10 230 30 233 3.90e-30 115
Msa0499950 MtrunA17_Chr4g0068111 32.719 217 98 6 37 236 112 297 9.80e-30 115
Msa0499950 MtrunA17_Chr5g0413271 29.146 199 107 3 39 232 145 314 1.29e-29 115
Msa0499950 MtrunA17_Chr4g0005481 30.882 204 104 4 35 230 104 278 3.25e-29 114
Msa0499950 MtrunA17_Chr8g0384151 31.481 216 98 6 39 234 90 275 2.79e-27 108
Msa0499950 MtrunA17_Chr8g0368461 27.801 241 117 6 20 232 155 366 1.59e-25 104
Msa0499950 MtrunA17_Chr1g0188191 29.907 214 104 6 28 230 223 401 1.05e-24 102
Msa0499950 MtrunA17_Chr6g0476041 36.975 119 46 1 38 156 166 255 6.72e-23 96.7
Msa0499950 MtrunA17_Chr5g0414051 38.136 118 44 1 39 156 75 163 7.40e-20 87.4
Msa0499950 MtrunA17_Chr5g0414061 28.141 199 108 4 39 231 94 263 1.07e-19 87.0
Msa0499950 MtrunA17_Chr8g0347321 37.398 123 48 1 39 161 95 188 2.44e-19 86.3
Msa0499950 MtrunA17_Chr5g0414091 29.064 203 108 4 36 231 82 255 1.24e-18 84.0
Msa0499950 MtrunA17_Chr2g0287911 52.941 68 25 1 26 93 48 108 1.34e-18 78.6
Msa0499950 MtrunA17_Chr5g0429211 52.000 75 36 0 122 196 194 268 1.59e-18 82.4
Msa0499950 MtrunA17_Chr8g0391481 29.648 199 101 5 39 230 27 193 4.85e-18 82.0
Msa0499950 MtrunA17_Chr7g0221291 35.593 118 47 1 39 156 79 167 1.27e-17 77.4
Msa0499950 MtrunA17_Chr7g0221271 35.593 118 47 1 39 156 79 167 8.04e-17 76.3
Msa0499950 MtrunA17_Chr7g0226991 28.218 202 108 5 36 231 54 224 1.21e-16 78.2
Msa0499950 MtrunA17_Chr3g0140371 29.851 201 102 5 37 231 16 183 4.22e-16 76.6
Msa0499950 MtrunA17_Chr5g0414041 27.885 208 114 5 34 234 82 260 9.06e-16 75.9
Msa0499950 MtrunA17_Chr8g0387921 33.898 118 49 1 39 156 74 162 7.19e-15 73.2
Msa0499950 MtrunA17_Chr8g0384421 27.700 213 105 8 38 230 132 315 4.52e-14 70.9
Msa0499950 MtrunA17_Chr6g0456681 31.967 122 51 2 26 147 54 143 4.23e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0499950 AT2G30900.2 50.000 230 83 4 12 238 15 215 5.91e-78 236
Msa0499950 AT2G30900.1 50.000 230 83 4 12 238 15 215 2.31e-76 235
Msa0499950 AT3G14850.2 50.980 204 71 1 35 238 30 204 1.30e-70 220
Msa0499950 AT2G42570.1 49.268 205 75 1 34 238 42 217 4.84e-66 209
Msa0499950 AT2G31110.3 45.259 232 94 2 11 238 13 215 7.39e-66 205
Msa0499950 AT1G29050.1 48.020 202 75 2 37 237 59 231 2.85e-65 207
Msa0499950 AT2G31110.2 45.259 232 94 2 11 238 13 215 2.47e-64 204
Msa0499950 AT2G34070.1 47.739 199 74 2 37 234 63 232 2.49e-62 200
Msa0499950 AT1G78710.2 50.249 201 67 4 39 237 31 200 7.41e-61 193
Msa0499950 AT1G78710.1 50.249 201 67 4 39 237 38 207 6.58e-60 192
Msa0499950 AT5G58600.2 44.118 204 83 3 36 237 63 237 1.07e-52 172
Msa0499950 AT5G58600.1 44.118 204 83 3 36 237 63 237 1.47e-51 172
Msa0499950 AT2G30010.1 43.564 202 81 3 37 234 54 226 1.28e-49 167
Msa0499950 AT2G30010.2 43.564 202 81 3 37 234 97 269 1.51e-49 168
Msa0499950 AT3G54260.1 38.693 199 90 4 39 235 51 219 7.99e-41 144
Msa0499950 AT5G19160.2 34.978 223 99 5 25 230 82 275 1.64e-40 142
Msa0499950 AT5G19160.1 34.978 223 99 5 25 230 82 275 2.10e-39 142
Msa0499950 AT3G14850.1 59.406 101 41 0 138 238 1 101 1.92e-37 132
Msa0499950 AT3G55990.1 33.019 212 105 4 30 233 131 313 2.12e-37 137
Msa0499950 AT1G73140.1 30.918 207 106 4 37 235 65 242 3.12e-37 135
Msa0499950 AT5G20590.2 34.450 209 94 5 38 232 140 319 1.74e-36 132
Msa0499950 AT2G40150.1 32.381 210 105 4 32 233 64 244 2.46e-36 133
Msa0499950 AT2G40320.1 34.112 214 103 5 31 236 73 256 4.25e-36 132
Msa0499950 AT3G06080.1 34.404 218 97 5 30 230 91 279 5.05e-36 130
Msa0499950 AT5G20590.1 34.450 209 94 5 38 232 140 319 8.03e-36 132
Msa0499950 AT3G62390.2 36.232 207 89 7 39 232 138 314 2.03e-35 130
Msa0499950 AT3G06080.2 34.404 218 97 5 30 230 91 279 2.17e-35 131
Msa0499950 AT3G62390.1 36.232 207 89 7 39 232 138 314 7.19e-35 130
Msa0499950 AT3G11030.1 31.308 214 109 5 31 236 98 281 1.56e-34 128
Msa0499950 AT2G40160.3 33.333 201 96 4 39 230 77 248 3.31e-34 125
Msa0499950 AT1G48880.1 35.577 208 89 5 39 231 115 292 9.57e-34 126
Msa0499950 AT5G01360.2 29.612 206 108 4 34 231 31 207 1.26e-33 125
Msa0499950 AT2G40160.2 33.831 201 95 4 39 230 77 248 2.26e-33 125
Msa0499950 AT2G40160.1 33.333 201 96 4 39 230 77 248 2.46e-33 125
Msa0499950 AT5G01360.1 29.612 206 108 4 34 231 87 263 2.56e-33 125
Msa0499950 AT5G49340.1 33.649 211 96 5 37 232 98 279 3.09e-33 125
Msa0499950 AT5G01620.2 32.075 212 106 4 28 230 99 281 6.93e-32 121
Msa0499950 AT5G01620.1 32.075 212 106 4 28 230 99 281 6.93e-32 121
Msa0499950 AT5G01620.3 32.075 212 106 4 28 230 107 289 1.09e-31 120
Msa0499950 AT3G12060.1 33.333 207 96 5 39 232 196 373 1.73e-31 121
Msa0499950 AT1G60790.1 31.455 213 102 5 39 236 190 373 1.23e-30 119
Msa0499950 AT5G06700.1 32.850 207 97 5 39 232 255 432 1.80e-30 119
Msa0499950 AT2G38320.1 33.488 215 102 6 28 230 46 231 2.43e-30 116
Msa0499950 AT2G34070.2 51.020 98 47 1 138 234 1 98 1.21e-27 106
Msa0499950 AT5G06230.3 32.394 213 93 6 39 230 62 244 3.69e-26 103
Msa0499950 AT5G06230.2 32.394 213 93 6 39 230 21 203 1.26e-25 103
Msa0499950 AT5G06230.1 32.394 213 93 6 39 230 62 244 1.80e-25 103
Msa0499950 AT5G51640.1 30.622 209 93 7 36 230 135 305 8.07e-23 96.7
Msa0499950 AT4G25360.1 31.100 209 94 8 35 230 171 342 1.02e-22 96.7
Msa0499950 AT4G25360.2 31.100 209 94 8 35 230 171 342 1.02e-22 96.7
Msa0499950 AT3G11570.1 32.461 191 84 6 55 230 98 258 8.49e-22 93.6
Msa0499950 AT5G15890.1 27.919 197 108 3 39 230 187 354 3.70e-19 86.3
Msa0499950 AT1G01430.1 38.983 118 43 1 39 156 99 187 1.03e-18 84.7
Msa0499950 AT1G70230.1 27.723 202 111 4 39 234 79 251 3.34e-18 83.2
Msa0499950 AT2G38320.2 29.379 177 87 4 63 230 1 148 1.79e-17 80.5
Msa0499950 AT2G37720.1 36.441 118 45 2 39 155 143 231 5.24e-17 80.1
Msa0499950 AT3G28150.1 35.833 120 48 1 39 158 70 160 1.52e-16 78.6
Msa0499950 AT5G64020.1 29.717 212 100 9 39 230 57 239 9.83e-16 75.9
Msa0499950 AT2G31110.1 58.621 58 24 0 181 238 10 67 1.00e-15 73.9
Msa0499950 AT4G11090.1 29.577 142 70 2 16 156 56 168 1.65e-14 72.4
Msa0499950 AT4G01080.1 33.607 122 52 1 35 156 87 179 4.09e-14 71.2

Find 114 sgRNAs with CRISPR-Local

Find 326 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTCCTTTCCAATTTC+TGG 0.179085 3_4:+39505033 None:intergenic
AACATCAAATGGATTTAAAT+TGG 0.188867 3_4:+39509397 None:intergenic
ATTTGTTTGTATATATCTTA+TGG 0.255186 3_4:-39504931 Msa0499950:three_prime_UTR
CTATTCAATCTGCCAGAAAT+TGG 0.256808 3_4:-39505045 Msa0499950:CDS
GATGGTCACCCATCAGTTTA+TGG 0.263279 3_4:-39503863 Msa0499950:three_prime_UTR
GTTCTCACGCAATGCGCTTC+TGG 0.267121 3_4:-39505113 Msa0499950:CDS
TTTGTAATAATGTGGGAGTT+AGG 0.295273 3_4:+39504868 None:intergenic
CATGTTCATGGGAAGACAAT+AGG 0.302907 3_4:-39509192 Msa0499950:CDS
ATGTGTTGATATTTGATTCT+TGG 0.304240 3_4:-39505012 Msa0499950:CDS
GTGATTTGTTTCAAGGGAAA+TGG 0.305553 3_4:-39509152 Msa0499950:CDS
CTTCTCATGATTCATGTTCA+TGG 0.309693 3_4:-39509204 Msa0499950:CDS
AGAGGACCAGTTGCAGAATA+TGG 0.317576 3_4:-39504801 Msa0499950:three_prime_UTR
TCTAACAGTGAAGGTGAAAA+TGG 0.328813 3_4:+39506176 None:intergenic
ATTGAATAGAGTCTAATTTC+AGG 0.342278 3_4:+39505059 None:intergenic
AAAAGCTGATTCCAAGTATC+AGG 0.342301 3_4:+39503788 None:intergenic
CCTTGGTAAGTTACAATTTG+TGG 0.350102 3_4:+39509011 None:intergenic
GAAAATATTCATATTTAGTA+AGG 0.353364 3_4:+39503709 None:intergenic
GAATTTGATTGTCAGAATAA+TGG 0.359636 3_4:-39509072 Msa0499950:CDS
TACTTAAGATAGAATTTATC+TGG 0.361064 3_4:+39509045 None:intergenic
TTGTAAAGGATGATGAAATA+AGG 0.361443 3_4:-39503660 Msa0499950:three_prime_UTR
TGACATGATTTGCAGACTTA+TGG 0.362689 3_4:-39505148 Msa0499950:intron
TCTTATTATACCCTCACATT+TGG 0.366557 3_4:-39504894 Msa0499950:three_prime_UTR
GTTATTTCAATAGACGTGTA+TGG 0.376680 3_4:-39507501 Msa0499950:intron
AGATTTATTTAAGTGGCTAT+TGG 0.377602 3_4:+39507444 None:intergenic
GACATTGTTAGTGAAAATAT+TGG 0.378386 3_4:-39505088 Msa0499950:CDS
CATTGAAAACATGGGCCAAA+TGG 0.378510 3_4:-39504664 Msa0499950:three_prime_UTR
CTTCACAAAAGTTTGCTGTT+GGG 0.379102 3_4:+39504023 None:intergenic
TTGTAATAATGTGGGAGTTA+GGG 0.383841 3_4:+39504869 None:intergenic
GGATACATGGATCCTGATAA+TGG 0.391224 3_4:+39503989 None:intergenic
AACTCCCACATTATTACAAA+TGG 0.392539 3_4:-39504865 Msa0499950:three_prime_UTR
ACTCTTTGAGTTTGAATCAA+TGG 0.394001 3_4:-39506272 Msa0499950:CDS
TGATTTGTTTCAAGGGAAAT+GGG 0.396652 3_4:-39509151 Msa0499950:CDS
TGCTAGTGAGTCACATGAAC+AGG 0.397889 3_4:+39509348 None:intergenic
GGAGAAGAGTTAACATCAAA+TGG 0.405650 3_4:+39509386 None:intergenic
CCTTCACAAAAGTTTGCTGT+TGG 0.406070 3_4:+39504022 None:intergenic
GATGATCTGGAGAGATTCCT+TGG 0.413038 3_4:+39504600 None:intergenic
AACATTGTCATCAACCCATT+TGG 0.413736 3_4:+39504649 None:intergenic
ATGTTCATGGGAAGACAATA+GGG 0.420069 3_4:-39509191 Msa0499950:CDS
AGTGGTTGTGATTTGTTTCA+AGG 0.421938 3_4:-39509159 Msa0499950:CDS
AATACTTACTTAAGATGATC+TGG 0.424664 3_4:+39504587 None:intergenic
AAAGCCAACTGTGCTGGAAC+TGG 0.426171 3_4:+39503959 None:intergenic
AATTCTATCTTAAGTATAGA+TGG 0.430323 3_4:-39509038 Msa0499950:CDS
TATTTCCAGCTCAAGGCAAT+GGG 0.432847 3_4:-39504050 Msa0499950:intron
AATTTATCATACAGATTCAA+TGG 0.435675 3_4:-39506345 Msa0499950:intron
GAATAACACATTCAGAGATA+TGG 0.437278 3_4:-39504711 Msa0499950:three_prime_UTR
CTTGGTAAGTTACAATTTGT+GGG 0.438766 3_4:+39509012 None:intergenic
CTCTCTAAAGCCAACTGTGC+TGG 0.439711 3_4:+39503953 None:intergenic
CACTGTGAGGTTTGATAAAG+AGG 0.440798 3_4:+39509111 None:intergenic
CATGAACAGGAAATACTATA+AGG 0.444186 3_4:+39509361 None:intergenic
ATAATAGAGATTTATTTAAG+TGG 0.448647 3_4:+39507437 None:intergenic
TGCAGCCATTGGTGTCTAGC+TGG 0.453171 3_4:-39503815 Msa0499950:three_prime_UTR
AACTGGACCTCCTGGATACA+TGG 0.456004 3_4:+39503976 None:intergenic
AAACAAATGAAGAATGTGTA+TGG 0.457392 3_4:+39504946 None:intergenic
GAAAGAACTACTCTTGGTAA+TGG 0.464536 3_4:-39507479 Msa0499950:CDS
TGATATTTGATTCTTGGCAT+TGG 0.464845 3_4:-39505006 Msa0499950:CDS
TAGCTGGTGTTCCTGATACT+TGG 0.468340 3_4:-39503799 Msa0499950:three_prime_UTR
TTTGCAGACTTATGGTGTTA+AGG 0.471271 3_4:-39505140 Msa0499950:intron
ATTGAAAACATGGGCCAAAT+GGG 0.475975 3_4:-39504663 Msa0499950:three_prime_UTR
TGTGCTGGAACTGGACCTCC+TGG 0.478514 3_4:+39503968 None:intergenic
ATGGTGAAGATTTCTTAAGA+AGG 0.480677 3_4:-39506326 Msa0499950:CDS
TTCTCATGATTCATGTTCAT+GGG 0.480734 3_4:-39509203 Msa0499950:CDS
TTTGTCCATTTGTAATAATG+TGG 0.482099 3_4:+39504860 None:intergenic
AAATCAATCAATATTTGTAA+AGG 0.501587 3_4:-39503674 Msa0499950:three_prime_UTR
GTGGTTGTGATTTGTTTCAA+GGG 0.506551 3_4:-39509158 Msa0499950:CDS
CACCCATCAGTTTATGGACA+TGG 0.508815 3_4:-39503857 Msa0499950:three_prime_UTR
AGTGGCTATTGGCTAACCAT+TGG 0.514275 3_4:+39507455 None:intergenic
AACTACACCTTAGTGAGGAT+TGG 0.516648 3_4:-39506207 Msa0499950:CDS
TTATCACAGCTGAGAAAAGA+TGG 0.516839 3_4:-39503881 Msa0499950:three_prime_UTR
TTGTTAGTGAAAATATTGGT+AGG 0.519394 3_4:-39505084 Msa0499950:CDS
ATCAATATTGTGTATGCAGA+TGG 0.522452 3_4:-39504751 Msa0499950:three_prime_UTR
GTAATGTCAAGCAAGTATAC+AGG 0.523286 3_4:+39503908 None:intergenic
ATATTTCCAGCTCAAGGCAA+TGG 0.526486 3_4:-39504051 Msa0499950:intron
TATTTGATTCTTGGCATTGG+TGG 0.527974 3_4:-39505003 Msa0499950:CDS
TTGTCCATTTGTAATAATGT+GGG 0.528867 3_4:+39504861 None:intergenic
AGATGGGATTTCATTCAAGA+AGG 0.534284 3_4:-39504734 Msa0499950:three_prime_UTR
ATGAGAAAGCATTGAAAACA+TGG 0.535255 3_4:-39504673 Msa0499950:three_prime_UTR
TACTCTTGGTAATGGACCAA+TGG 0.541057 3_4:-39507471 Msa0499950:CDS
TGTAATAATGTGGGAGTTAG+GGG 0.546845 3_4:+39504870 None:intergenic
GGGACATGGATTGCAGCCAT+TGG 0.548321 3_4:-39503826 Msa0499950:three_prime_UTR
TGTATGGAAAGAACTACTCT+TGG 0.548582 3_4:-39507485 Msa0499950:CDS
ATTTCTTAAGAAGGCATAGA+GGG 0.548849 3_4:-39506317 Msa0499950:CDS
CTCACGCAATGCGCTTCTGG+TGG 0.553358 3_4:-39505110 Msa0499950:CDS
TTATCAGGATCCATGTATCC+AGG 0.553563 3_4:-39503986 Msa0499950:three_prime_UTR
AGGTCCAGTTCCAGCACAGT+TGG 0.553722 3_4:-39503963 Msa0499950:three_prime_UTR
CCTCCATGTCCATAAACTGA+TGG 0.556684 3_4:+39503854 None:intergenic
TTATGGACATGGAGGACATA+GGG 0.558140 3_4:-39503846 Msa0499950:three_prime_UTR
GATGGGATTTCATTCAAGAA+GGG 0.559009 3_4:-39504733 Msa0499950:three_prime_UTR
TATGATGATAGATTTCATGC+TGG 0.559751 3_4:+39509248 None:intergenic
GATTTCTTAAGAAGGCATAG+AGG 0.560528 3_4:-39506318 Msa0499950:CDS
ACAATAGGGTTTGCAAAGAG+TGG 0.562019 3_4:-39509177 Msa0499950:CDS
GAGATCACCAATCCTCACTA+AGG 0.570198 3_4:+39506200 None:intergenic
TTTATGGACATGGAGGACAT+AGG 0.578389 3_4:-39503847 Msa0499950:three_prime_UTR
TGTTAGTGAAAATATTGGTA+GGG 0.582185 3_4:-39505083 Msa0499950:CDS
AACAGGAAATACTATAAGGA+GGG 0.590528 3_4:+39509365 None:intergenic
TAGCTTGTGGTACAGCTATG+TGG 0.590974 3_4:+39506229 None:intergenic
TTCTATAAAGGGACACTGTG+AGG 0.595117 3_4:+39509098 None:intergenic
GAACAGGAAATACTATAAGG+AGG 0.598443 3_4:+39509364 None:intergenic
TGAGAAAGCATTGAAAACAT+GGG 0.607788 3_4:-39504672 Msa0499950:three_prime_UTR
CTCCATGTCCATAAACTGAT+GGG 0.611052 3_4:+39503855 None:intergenic
GAGTTAGGGGACCAAATGTG+AGG 0.611255 3_4:+39504883 None:intergenic
ACTAAGGTGTAGTTAGCTTG+TGG 0.614706 3_4:+39506216 None:intergenic
CAATCTGCCAGAAATTGGAA+AGG 0.615695 3_4:-39505040 Msa0499950:CDS
GGATAGAGATCTAACAGTGA+AGG 0.619088 3_4:+39506167 None:intergenic
TGGCATTGGTGGCTTCACAC+AGG 0.631401 3_4:-39504992 Msa0499950:CDS
CCACAAATTGTAACTTACCA+AGG 0.640371 3_4:-39509011 Msa0499950:intron
TGTTGATATTACCAAAACAA+AGG 0.642467 3_4:-39504630 Msa0499950:three_prime_UTR
TCAATATTGTGTATGCAGAT+GGG 0.645753 3_4:-39504750 Msa0499950:three_prime_UTR
GAACACCAGCTAGACACCAA+TGG 0.667103 3_4:+39503810 None:intergenic
AGTTAGGGGACCAAATGTGA+GGG 0.672404 3_4:+39504884 None:intergenic
ACATGGAGGACATAGGGACA+TGG 0.684758 3_4:-39503840 Msa0499950:three_prime_UTR
CCATCAGTTTATGGACATGG+AGG 0.696347 3_4:-39503854 Msa0499950:three_prime_UTR
AAGCTAACTACACCTTAGTG+AGG 0.700717 3_4:-39506212 Msa0499950:CDS
ATTTCCAGCTCAAGGCAATG+GGG 0.709934 3_4:-39504049 Msa0499950:intron
TCAGGATCCATGTATCCAGG+AGG 0.744104 3_4:-39503983 Msa0499950:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTAATATTAATAGTAAG+AGG + chr3_4:39505292-39505311 None:intergenic 10.0%
!!! AAAATATATAATCACTATTT+TGG + chr3_4:39505316-39505335 None:intergenic 10.0%
!!! AAAAAGATACTTTTAAATTT+AGG - chr3_4:39505583-39505602 Msa0499950:intron 10.0%
!!! GTTCTTTTTTTTTTTTTTTT+TGG - chr3_4:39506305-39506324 Msa0499950:CDS 10.0%
!!! CACTTTTTTTTTTTTTTTTT+CGG - chr3_4:39506842-39506861 Msa0499950:intron 10.0%
!! AAACTTATTTAAGAATAATT+TGG + chr3_4:39507184-39507203 None:intergenic 10.0%
!!! ATCAAAATCATTATATTTTT+AGG - chr3_4:39507523-39507542 Msa0499950:intron 10.0%
!!! TCAAAATCATTATATTTTTA+GGG - chr3_4:39507524-39507543 Msa0499950:intron 10.0%
!! AAAAAAAAAAACTTCAAATT+AGG + chr3_4:39508464-39508483 None:intergenic 10.0%
!!! CCAAAAAATTTAAAATAAAT+AGG + chr3_4:39508500-39508519 None:intergenic 10.0%
!!! CCTATTTATTTTAAATTTTT+TGG - chr3_4:39508497-39508516 Msa0499950:intron 10.0%
!!! AACAAATTTTTTTTTTTTGA+AGG + chr3_4:39508695-39508714 None:intergenic 10.0%
!!! TTTTTATTTGATCTATATAT+AGG - chr3_4:39509229-39509248 Msa0499950:CDS 10.0%
!!! AATATATATGATGAAATTTT+AGG - chr3_4:39509270-39509289 Msa0499950:CDS 10.0%
!!! TCAAATTCTTTTTCTATAAA+GGG + chr3_4:39503756-39503775 None:intergenic 15.0%
!!! ATCAAATTCTTTTTCTATAA+AGG + chr3_4:39503757-39503776 None:intergenic 15.0%
!!! AATTAATAACAGTTCTAAAT+TGG - chr3_4:39504145-39504164 Msa0499950:intron 15.0%
!!! CACAAATTTTTTTTAAGTTA+GGG + chr3_4:39504558-39504577 None:intergenic 15.0%
!! CTAACTTAAAAAAAATTTGT+GGG - chr3_4:39504557-39504576 Msa0499950:intron 15.0%
!!! TCTCTAATGATTTTTTTTTT+AGG - chr3_4:39504615-39504634 Msa0499950:three_prime_UTR 15.0%
!! AACAAAAATGAAGTATAATA+AGG + chr3_4:39504662-39504681 None:intergenic 15.0%
!! TTTATGAAATTAAAACCATA+GGG - chr3_4:39504680-39504699 Msa0499950:three_prime_UTR 15.0%
!! AATCTAAAGGAAATAAAAAT+TGG - chr3_4:39504895-39504914 Msa0499950:three_prime_UTR 15.0%
!! ACAAAAAAAATAAAAATCGA+AGG + chr3_4:39504936-39504955 None:intergenic 15.0%
!! ATATTTCATATTATATGCAA+GGG + chr3_4:39505030-39505049 None:intergenic 15.0%
!! ATAATAGAGATTTATTTAAG+TGG + chr3_4:39505406-39505425 None:intergenic 15.0%
!! TTTATCTATCACAAAATAAA+GGG - chr3_4:39505662-39505681 Msa0499950:intron 15.0%
!!! CTTACACTTTTTATTTTTTT+TGG + chr3_4:39505826-39505845 None:intergenic 15.0%
!!! CAAAAAAAATAAAAAGTGTA+AGG - chr3_4:39505824-39505843 Msa0499950:intron 15.0%
!!! TTGTTTGTTGAATAATTATT+TGG + chr3_4:39505957-39505976 None:intergenic 15.0%
!!! TTGTAATTTTGATACATTTA+AGG + chr3_4:39505996-39506015 None:intergenic 15.0%
!! TTGATTTAGTCTATAAAATT+AGG - chr3_4:39506107-39506126 Msa0499950:intron 15.0%
!! TGATTTAGTCTATAAAATTA+GGG - chr3_4:39506108-39506127 Msa0499950:intron 15.0%
!! TTTCATTCAAAAATATTAAG+TGG + chr3_4:39506477-39506496 None:intergenic 15.0%
!! AAGAAATGAAAAAATGTTAT+AGG + chr3_4:39506697-39506716 None:intergenic 15.0%
!!! TAAAACTACTTGTTATTTAA+CGG - chr3_4:39506893-39506912 Msa0499950:intron 15.0%
!! TAAAAAAGAAACAAATGATT+TGG + chr3_4:39507061-39507080 None:intergenic 15.0%
!!! TCTTTTTTATAAAACGAAAT+TGG - chr3_4:39507072-39507091 Msa0499950:intron 15.0%
!! ATGTTAAAATTATGTTCAAT+AGG + chr3_4:39507310-39507329 None:intergenic 15.0%
!! TCTTGAACATAATAATAATA+TGG - chr3_4:39507332-39507351 Msa0499950:intron 15.0%
!! AAAATGAAACAAAAAATTTG+AGG - chr3_4:39507461-39507480 Msa0499950:CDS 15.0%
!!! AACATCTCATTTTAATTTTT+AGG - chr3_4:39507493-39507512 Msa0499950:intron 15.0%
!!! ACATCTCATTTTAATTTTTA+GGG - chr3_4:39507494-39507513 Msa0499950:intron 15.0%
!!! TTCAAATGAAAATTTTAACT+TGG - chr3_4:39507594-39507613 Msa0499950:intron 15.0%
!! ATTTGTTTGTATATATCTTA+TGG - chr3_4:39507909-39507928 Msa0499950:intron 15.0%
!! TCATTAAATTACTTTCAAAA+TGG + chr3_4:39508349-39508368 None:intergenic 15.0%
!! GAAAATATTCATATTTAGTA+AGG + chr3_4:39509134-39509153 None:intergenic 15.0%
!! AAATCAATCAATATTTGTAA+AGG - chr3_4:39509166-39509185 Msa0499950:CDS 15.0%
!!! AACATCAAATGGATTTAAAT+TGG + chr3_4:39503446-39503465 None:intergenic 20.0%
!! TACTTAAGATAGAATTTATC+TGG + chr3_4:39503798-39503817 None:intergenic 20.0%
!! AATTCTATCTTAAGTATAGA+TGG - chr3_4:39503802-39503821 Msa0499950:three_prime_UTR 20.0%
!! ATACTAGGAAATATTAATGT+TGG + chr3_4:39504174-39504193 None:intergenic 20.0%
!! AGTTTATTCTGACATATAAT+AGG - chr3_4:39504274-39504293 Msa0499950:intron 20.0%
!!! TTTTGTAAAGATTTCTGATA+TGG - chr3_4:39504335-39504354 Msa0499950:intron 20.0%
!!! CCACAAATTTTTTTTAAGTT+AGG + chr3_4:39504559-39504578 None:intergenic 20.0%
!! CCTAACTTAAAAAAAATTTG+TGG - chr3_4:39504556-39504575 Msa0499950:intron 20.0%
!! GTTTATGAAATTAAAACCAT+AGG - chr3_4:39504679-39504698 Msa0499950:three_prime_UTR 20.0%
!!! TTTTTTTGTTTTTTTCCCTA+TGG + chr3_4:39504698-39504717 None:intergenic 20.0%
!! GGGAAAAAAACAAAAAAAAT+AGG - chr3_4:39504700-39504719 Msa0499950:three_prime_UTR 20.0%
!! GATATTTCATATTATATGCA+AGG + chr3_4:39505031-39505050 None:intergenic 20.0%
!!! TTATAACGGTTTTGTTATTT+TGG + chr3_4:39505180-39505199 None:intergenic 20.0%
!!! ATTTAAAAGTATCTTTTTGC+TGG + chr3_4:39505581-39505600 None:intergenic 20.0%
!! ACAAATATATACTTGTCAAA+TGG + chr3_4:39505631-39505650 None:intergenic 20.0%
!! ATTTGACAAGTATATATTTG+TGG - chr3_4:39505630-39505649 Msa0499950:intron 20.0%
!! TTTGACAAGTATATATTTGT+GGG - chr3_4:39505631-39505650 Msa0499950:intron 20.0%
!! GTTTATCTATCACAAAATAA+AGG - chr3_4:39505661-39505680 Msa0499950:intron 20.0%
!! TTATCTATCACAAAATAAAG+GGG - chr3_4:39505663-39505682 Msa0499950:intron 20.0%
!! TAAATAAACAACTTCTAACT+TGG - chr3_4:39505703-39505722 Msa0499950:intron 20.0%
!! AAATAAACAACTTCTAACTT+GGG - chr3_4:39505704-39505723 Msa0499950:intron 20.0%
!! AACAACCTTTAATTGATATT+GGG + chr3_4:39506210-39506229 None:intergenic 20.0%
!! TAACAACCTTTAATTGATAT+TGG + chr3_4:39506211-39506230 None:intergenic 20.0%
!! AATATCAATTAAAGGTTGTT+AGG - chr3_4:39506210-39506229 Msa0499950:CDS 20.0%
!!! TTTTTTTTTGGAAATATGTG+TGG - chr3_4:39506317-39506336 Msa0499950:CDS 20.0%
!!! CATTAAATGGTAATTTTGTT+CGG + chr3_4:39506425-39506444 None:intergenic 20.0%
!! TTACCATTTAATGATGATTA+AGG - chr3_4:39506432-39506451 Msa0499950:intron 20.0%
!! AATTTATCATACAGATTCAA+TGG - chr3_4:39506495-39506514 Msa0499950:intron 20.0%
!! CACATAAAATGAAAAATAGA+TGG - chr3_4:39506919-39506938 Msa0499950:intron 20.0%
!! ATAAAATGAAAAATAGATGG+AGG - chr3_4:39506922-39506941 Msa0499950:intron 20.0%
!!! CAAAACAAATTTTTTTGTGA+GGG + chr3_4:39507279-39507298 None:intergenic 20.0%
!!! TCAAAACAAATTTTTTTGTG+AGG + chr3_4:39507280-39507299 None:intergenic 20.0%
!! CATACATAGAACATTATAAA+TGG - chr3_4:39507356-39507375 Msa0499950:intron 20.0%
!! TGAACATAAATTTGTCTTTA+TGG - chr3_4:39507434-39507453 Msa0499950:CDS 20.0%
!!! AAATCATTATATTTTTAGGG+AGG - chr3_4:39507527-39507546 Msa0499950:intron 20.0%
!!! ATGTGTATGGATTTTAATTA+CGG + chr3_4:39507884-39507903 None:intergenic 20.0%
!! ATTAGGATGTAAATTTCTTT+AGG - chr3_4:39508317-39508336 Msa0499950:intron 20.0%
!! TTAGGATGTAAATTTCTTTA+GGG - chr3_4:39508318-39508337 Msa0499950:intron 20.0%
!!! TTTAAATTTTTTGGGACAAA+AGG - chr3_4:39508506-39508525 Msa0499950:intron 20.0%
!!! AAAATGTTATCTCCTTTTAA+TGG + chr3_4:39508660-39508679 None:intergenic 20.0%
! GACAATCAATAAAAAAGAAG+CGG + chr3_4:39503553-39503572 None:intergenic 25.0%
! GAATTTGATTGTCAGAATAA+TGG - chr3_4:39503768-39503787 Msa0499950:three_prime_UTR 25.0%
! ATCGTTAAAAGACATACTTA+AGG - chr3_4:39503910-39503929 Msa0499950:three_prime_UTR 25.0%
! AAAATCATGCATCTTTACAA+TGG + chr3_4:39504086-39504105 None:intergenic 25.0%
!! GTCAGAATAAACTAATTTTG+TGG + chr3_4:39504267-39504286 None:intergenic 25.0%
! AATAGATTAAAGTAACTGCT+AGG + chr3_4:39504300-39504319 None:intergenic 25.0%
! CTTTAATCTATTTACTCTGA+AGG - chr3_4:39504308-39504327 Msa0499950:intron 25.0%
!! TTTAAGTTAGGGAGTAAAAA+AGG + chr3_4:39504547-39504566 None:intergenic 25.0%
!! AGTAAAATATCACATGGTTT+TGG + chr3_4:39504584-39504603 None:intergenic 25.0%
! TCACTAAGTAAAATATCACA+TGG + chr3_4:39504590-39504609 None:intergenic 25.0%
! GTATAATAAGGTAACATCAA+TGG + chr3_4:39504650-39504669 None:intergenic 25.0%
!! AGCTTGATCTTTTAATCTAA+AGG - chr3_4:39504882-39504901 Msa0499950:three_prime_UTR 25.0%
! ATATGTGTGTGAGTTTATAA+CGG + chr3_4:39505194-39505213 None:intergenic 25.0%
! AGATTTATTTAAGTGGCTAT+TGG + chr3_4:39505399-39505418 None:intergenic 25.0%
!! GATAAGTATGCTTTCATTTT+CGG - chr3_4:39505434-39505453 Msa0499950:intron 25.0%
! TTGTTGAGTAATGTTAACTA+GGG + chr3_4:39505769-39505788 None:intergenic 25.0%
! ATTGTTGAGTAATGTTAACT+AGG + chr3_4:39505770-39505789 None:intergenic 25.0%
! TAGCAAACAAAGTAATGATT+TGG + chr3_4:39505909-39505928 None:intergenic 25.0%
!! AAATCATTACTTTGTTTGCT+AGG - chr3_4:39505908-39505927 Msa0499950:intron 25.0%
!!! TCTTTGAATGTGTTTTTTGT+TGG - chr3_4:39506084-39506103 Msa0499950:intron 25.0%
!! AATTTTGTTCGGATCTAATA+TGG + chr3_4:39506414-39506433 None:intergenic 25.0%
!!! GGAAAAGTATTTTGTTTGTT+GGG - chr3_4:39506544-39506563 Msa0499950:intron 25.0%
!!! GAAAAGTATTTTGTTTGTTG+GGG - chr3_4:39506545-39506564 Msa0499950:intron 25.0%
!! TTATGATAATGAGACAGAAT+TGG + chr3_4:39506728-39506747 None:intergenic 25.0%
!!! ACTTGTTATCCTAATAAATG+TGG - chr3_4:39506990-39507009 Msa0499950:intron 25.0%
! TCATTTGATCCACATTTATT+AGG + chr3_4:39507002-39507021 None:intergenic 25.0%
! AAATGTGGATCAAATGAATA+TGG - chr3_4:39507005-39507024 Msa0499950:intron 25.0%
! TTATAAAACGAAATTGGTGA+AGG - chr3_4:39507078-39507097 Msa0499950:intron 25.0%
!! AAATTCGCTATATTTTCAGA+AGG + chr3_4:39507214-39507233 None:intergenic 25.0%
! TGAAACAAAAAATTTGAGGA+AGG - chr3_4:39507465-39507484 Msa0499950:CDS 25.0%
!! TTAACTTGGAGTTCATTTAT+TGG - chr3_4:39507608-39507627 Msa0499950:intron 25.0%
! GACATTGTTAGTGAAAATAT+TGG - chr3_4:39507752-39507771 Msa0499950:intron 25.0%
! TTGTTAGTGAAAATATTGGT+AGG - chr3_4:39507756-39507775 Msa0499950:intron 25.0%
! TGTTAGTGAAAATATTGGTA+GGG - chr3_4:39507757-39507776 Msa0499950:intron 25.0%
! ATTGAATAGAGTCTAATTTC+AGG + chr3_4:39507784-39507803 None:intergenic 25.0%
!! ATGTGTTGATATTTGATTCT+TGG - chr3_4:39507828-39507847 Msa0499950:intron 25.0%
! AAACAAATGAAGAATGTGTA+TGG + chr3_4:39507897-39507916 None:intergenic 25.0%
! TTGTCCATTTGTAATAATGT+GGG + chr3_4:39507982-39508001 None:intergenic 25.0%
! TTTGTCCATTTGTAATAATG+TGG + chr3_4:39507983-39508002 None:intergenic 25.0%
! TGTTGATATTACCAAAACAA+AGG - chr3_4:39508210-39508229 Msa0499950:intron 25.0%
! AATACTTACTTAAGATGATC+TGG + chr3_4:39508256-39508275 None:intergenic 25.0%
! GAGATGAAAATTGCATAATT+AGG - chr3_4:39508300-39508319 Msa0499950:intron 25.0%
! GCATGTACAAAATTTAAAAG+TGG + chr3_4:39508437-39508456 None:intergenic 25.0%
!! AATGGTAAAAGTACAACAAT+GGG + chr3_4:39508642-39508661 None:intergenic 25.0%
!! TAATGGTAAAAGTACAACAA+TGG + chr3_4:39508643-39508662 None:intergenic 25.0%
!! GTTGTACTTTTACCATTAAA+AGG - chr3_4:39508645-39508664 Msa0499950:intron 25.0%
! TTGTAAAGGATGATGAAATA+AGG - chr3_4:39509180-39509199 Msa0499950:CDS 25.0%
!!! TCAAATTTTTGGTGGTATAT+TGG + chr3_4:39509379-39509398 None:intergenic 25.0%
!! AATATGCCTTGTCAAATTTT+TGG + chr3_4:39509390-39509409 None:intergenic 25.0%
AACAGGAAATACTATAAGGA+GGG + chr3_4:39503478-39503497 None:intergenic 30.0%
CATGAACAGGAAATACTATA+AGG + chr3_4:39503482-39503501 None:intergenic 30.0%
! GGTTTGCAATTTTTCTGATA+TGG + chr3_4:39503532-39503551 None:intergenic 30.0%
TATGATGATAGATTTCATGC+TGG + chr3_4:39503595-39503614 None:intergenic 30.0%
TTCTCATGATTCATGTTCAT+GGG - chr3_4:39503637-39503656 Msa0499950:three_prime_UTR 30.0%
!! TGATTTGTTTCAAGGGAAAT+GGG - chr3_4:39503689-39503708 Msa0499950:three_prime_UTR 30.0%
CTTGGTAAGTTACAATTTGT+GGG + chr3_4:39503831-39503850 None:intergenic 30.0%
TAAAAGAAATGTGTGTACCT+TGG + chr3_4:39503849-39503868 None:intergenic 30.0%
TTAAGGTATGAATCGTGATT+AGG - chr3_4:39503927-39503946 Msa0499950:three_prime_UTR 30.0%
TTATGTCACCATCTAAATTG+TGG - chr3_4:39504038-39504057 Msa0499950:three_prime_UTR 30.0%
!! GGTGGCTAAGATTTTTAAAA+CGG - chr3_4:39504059-39504078 Msa0499950:intron 30.0%
!! TAAAGATGCATGATTTTAGC+TGG - chr3_4:39504089-39504108 Msa0499950:intron 30.0%
TCCTAGTATAATTGCATATG+TGG - chr3_4:39504185-39504204 Msa0499950:intron 30.0%
TCCACATATGCAATTATACT+AGG + chr3_4:39504189-39504208 None:intergenic 30.0%
AAGAGACATCATCTATTACT+TGG + chr3_4:39504499-39504518 None:intergenic 30.0%
GAAGATTTCACAATCTATCA+TGG - chr3_4:39504781-39504800 Msa0499950:three_prime_UTR 30.0%
AGAACTCACACAATTAAATG+TGG - chr3_4:39504973-39504992 Msa0499950:exon 30.0%
ACAATTAAATGTGGAGAAGT+GGG - chr3_4:39504982-39505001 Msa0499950:CDS 30.0%
ATGAACCTTCGTTCTTAATT+TGG - chr3_4:39505078-39505097 Msa0499950:CDS 30.0%
TGAACCTTCGTTCTTAATTT+GGG - chr3_4:39505079-39505098 Msa0499950:CDS 30.0%
TATAAACTCACACACATATG+TGG - chr3_4:39505195-39505214 Msa0499950:intron 30.0%
GCATACACTATGATTGATTA+AGG + chr3_4:39505242-39505261 None:intergenic 30.0%
! GTTATTTCAATAGACGTGTA+TGG - chr3_4:39505339-39505358 Msa0499950:intron 30.0%
! CAAAGTGTCTTGATGATAAT+TGG + chr3_4:39505868-39505887 None:intergenic 30.0%
CACTCACACATTCAAATAAA+TGG - chr3_4:39506182-39506201 Msa0499950:CDS 30.0%
! TGGCCTTAATCATCATTAAA+TGG + chr3_4:39506438-39506457 None:intergenic 30.0%
ATGGTGAAGATTTCTTAAGA+AGG - chr3_4:39506514-39506533 Msa0499950:intron 30.0%
! ATTTCTTAAGAAGGCATAGA+GGG - chr3_4:39506523-39506542 Msa0499950:intron 30.0%
!!! GGGAAAAGTATTTTGTTTGT+TGG - chr3_4:39506543-39506562 Msa0499950:intron 30.0%
! ACTCTTTGAGTTTGAATCAA+TGG - chr3_4:39506568-39506587 Msa0499950:intron 30.0%
! TGAATATGGTCTCTTTTACA+TGG - chr3_4:39507019-39507038 Msa0499950:intron 30.0%
ATTTGGCATTGCAATGTTAT+TGG + chr3_4:39507167-39507186 None:intergenic 30.0%
!!! AACAAATTTTTTTGTGAGGG+TGG + chr3_4:39507276-39507295 None:intergenic 30.0%
!!! CTAGTCGTAGTTTTGATTTA+GGG + chr3_4:39507567-39507586 None:intergenic 30.0%
!!! TCTAGTCGTAGTTTTGATTT+AGG + chr3_4:39507568-39507587 None:intergenic 30.0%
ATCAATTCCTTTCCAATTTC+TGG + chr3_4:39507810-39507829 None:intergenic 30.0%
TGATATTTGATTCTTGGCAT+TGG - chr3_4:39507834-39507853 Msa0499950:intron 30.0%
TCTTATTATACCCTCACATT+TGG - chr3_4:39507946-39507965 Msa0499950:intron 30.0%
TTGTAATAATGTGGGAGTTA+GGG + chr3_4:39507974-39507993 None:intergenic 30.0%
TTTGTAATAATGTGGGAGTT+AGG + chr3_4:39507975-39507994 None:intergenic 30.0%
AACTCCCACATTATTACAAA+TGG - chr3_4:39507975-39507994 Msa0499950:intron 30.0%
ATCAATATTGTGTATGCAGA+TGG - chr3_4:39508089-39508108 Msa0499950:intron 30.0%
TCAATATTGTGTATGCAGAT+GGG - chr3_4:39508090-39508109 Msa0499950:intron 30.0%
GAATAACACATTCAGAGATA+TGG - chr3_4:39508129-39508148 Msa0499950:intron 30.0%
!! ATGAGAAAGCATTGAAAACA+TGG - chr3_4:39508167-39508186 Msa0499950:intron 30.0%
!! TGAGAAAGCATTGAAAACAT+GGG - chr3_4:39508168-39508187 Msa0499950:intron 30.0%
!! TTGGAAAAAGACCTTTGTTT+TGG + chr3_4:39508224-39508243 None:intergenic 30.0%
! AAAACAAAGGTCTTTTTCCA+AGG - chr3_4:39508223-39508242 Msa0499950:intron 30.0%
ACAAAAGGATCCTTTCAATA+TGG - chr3_4:39508521-39508540 Msa0499950:intron 30.0%
CAAAAGGATCCTTTCAATAT+GGG - chr3_4:39508522-39508541 Msa0499950:intron 30.0%
TTCAAAATATTTCCAGCTCA+AGG - chr3_4:39508783-39508802 Msa0499950:intron 30.0%
ATACCACCAAAAATTTGACA+AGG - chr3_4:39509381-39509400 Msa0499950:five_prime_UTR 30.0%
GGAGAAGAGTTAACATCAAA+TGG + chr3_4:39503457-39503476 None:intergenic 35.0%
GAACAGGAAATACTATAAGG+AGG + chr3_4:39503479-39503498 None:intergenic 35.0%
CTTCTCATGATTCATGTTCA+TGG - chr3_4:39503636-39503655 Msa0499950:three_prime_UTR 35.0%
ATGTTCATGGGAAGACAATA+GGG - chr3_4:39503649-39503668 Msa0499950:three_prime_UTR 35.0%
!! AGTGGTTGTGATTTGTTTCA+AGG - chr3_4:39503681-39503700 Msa0499950:three_prime_UTR 35.0%
!! GTGGTTGTGATTTGTTTCAA+GGG - chr3_4:39503682-39503701 Msa0499950:three_prime_UTR 35.0%
!! GTGATTTGTTTCAAGGGAAA+TGG - chr3_4:39503688-39503707 Msa0499950:three_prime_UTR 35.0%
CCTTGGTAAGTTACAATTTG+TGG + chr3_4:39503832-39503851 None:intergenic 35.0%
CCACAAATTGTAACTTACCA+AGG - chr3_4:39503829-39503848 Msa0499950:three_prime_UTR 35.0%
CTTGATATTGCAGAGAATTG+TGG - chr3_4:39503964-39503983 Msa0499950:three_prime_UTR 35.0%
AAGTTAGGGAGTAAAAAAGG+CGG + chr3_4:39504544-39504563 None:intergenic 35.0%
!!! TTTTTTGTCAAGTAGCCTAG+TGG - chr3_4:39504948-39504967 Msa0499950:three_prime_UTR 35.0%
CACAATTAAATGTGGAGAAG+TGG - chr3_4:39504981-39505000 Msa0499950:CDS 35.0%
CAATTAAATGTGGAGAAGTG+GGG - chr3_4:39504983-39505002 Msa0499950:CDS 35.0%
TTCATATTATATGCAAGGGC+CGG + chr3_4:39505026-39505045 None:intergenic 35.0%
! TGAGTTTAACTCAGTTGGTA+TGG + chr3_4:39505053-39505072 None:intergenic 35.0%
TACCAACTGAGTTAAACTCA+CGG - chr3_4:39505053-39505072 Msa0499950:CDS 35.0%
ATTAAGGTTCCCTGGAATAA+TGG + chr3_4:39505226-39505245 None:intergenic 35.0%
TATGATTGATTAAGGTTCCC+TGG + chr3_4:39505234-39505253 None:intergenic 35.0%
TGTATGGAAAGAACTACTCT+TGG - chr3_4:39505355-39505374 Msa0499950:intron 35.0%
GAAAGAACTACTCTTGGTAA+TGG - chr3_4:39505361-39505380 Msa0499950:intron 35.0%
ACAACTTCTAACTTGGGAAA+AGG - chr3_4:39505710-39505729 Msa0499950:intron 35.0%
GGTAAGATGAAAAACGAGTT+AGG + chr3_4:39505805-39505824 None:intergenic 35.0%
! AGTGTCTTGATGATAATTGG+AGG + chr3_4:39505865-39505884 None:intergenic 35.0%
! TTTGCTAGGCTGTCTTTTTA+AGG - chr3_4:39505922-39505941 Msa0499950:intron 35.0%
TGGCACCCAATATCAATTAA+AGG - chr3_4:39506202-39506221 Msa0499950:CDS 35.0%
ATTCATACTTGAACCACCAT+AGG - chr3_4:39506266-39506285 Msa0499950:CDS 35.0%
TTATCCACACATACATCCTA+TGG + chr3_4:39506285-39506304 None:intergenic 35.0%
! GATTTCTTAAGAAGGCATAG+AGG - chr3_4:39506522-39506541 Msa0499950:intron 35.0%
TCTAACAGTGAAGGTGAAAA+TGG + chr3_4:39506667-39506686 None:intergenic 35.0%
GATAATGAGACAGAATTGGA+AGG + chr3_4:39506724-39506743 None:intergenic 35.0%
GAGCTAGCTCAAATAATTGA+GGG + chr3_4:39506793-39506812 None:intergenic 35.0%
TGAGCTAGCTCAAATAATTG+AGG + chr3_4:39506794-39506813 None:intergenic 35.0%
CAATTATTTGAGCTAGCTCA+TGG - chr3_4:39506794-39506813 Msa0499950:intron 35.0%
TAGGATAACAAGTTGTCTCA+TGG + chr3_4:39506983-39507002 None:intergenic 35.0%
! CATTTTACAATTATGCCGAG+AGG - chr3_4:39507135-39507154 Msa0499950:intron 35.0%
TGTTATTGGACAAATCCTCT+CGG + chr3_4:39507153-39507172 None:intergenic 35.0%
! TGACATGATTTGCAGACTTA+TGG - chr3_4:39507692-39507711 Msa0499950:intron 35.0%
! TTTGCAGACTTATGGTGTTA+AGG - chr3_4:39507700-39507719 Msa0499950:intron 35.0%
CTATTCAATCTGCCAGAAAT+TGG - chr3_4:39507795-39507814 Msa0499950:intron 35.0%
! TATTTGATTCTTGGCATTGG+TGG - chr3_4:39507837-39507856 Msa0499950:intron 35.0%
TGTAATAATGTGGGAGTTAG+GGG + chr3_4:39507973-39507992 None:intergenic 35.0%
GGAAAAAATGTTGATGTGAG+AGG - chr3_4:39508021-39508040 Msa0499950:intron 35.0%
! TGTTTTCCATATTCTGCAAC+TGG + chr3_4:39508048-39508067 None:intergenic 35.0%
AGATGGGATTTCATTCAAGA+AGG - chr3_4:39508106-39508125 Msa0499950:intron 35.0%
GATGGGATTTCATTCAAGAA+GGG - chr3_4:39508107-39508126 Msa0499950:intron 35.0%
ATTGAAAACATGGGCCAAAT+GGG - chr3_4:39508177-39508196 Msa0499950:intron 35.0%
AACATTGTCATCAACCCATT+TGG + chr3_4:39508194-39508213 None:intergenic 35.0%
AAAGTAGAGCCCATATTGAA+AGG + chr3_4:39508534-39508553 None:intergenic 35.0%
!! CTTCACAAAAGTTTGCTGTT+GGG + chr3_4:39508820-39508839 None:intergenic 35.0%
GGACAAGAAAAACCATTATC+AGG - chr3_4:39508839-39508858 Msa0499950:intron 35.0%
!!! CAAGTATACAGGCTTTTTCA+TGG + chr3_4:39508924-39508943 None:intergenic 35.0%
GTAATGTCAAGCAAGTATAC+AGG + chr3_4:39508935-39508954 None:intergenic 35.0%
! TTTTCTCAGCTGTGATAAAG+TGG + chr3_4:39508957-39508976 None:intergenic 35.0%
TTATCACAGCTGAGAAAAGA+TGG - chr3_4:39508959-39508978 Msa0499950:intron 35.0%
! AAAAGCTGATTCCAAGTATC+AGG + chr3_4:39509055-39509074 None:intergenic 35.0%
!! ATGCCTTGTCAAATTTTTGG+TGG + chr3_4:39509387-39509406 None:intergenic 35.0%
CATGTTCATGGGAAGACAAT+AGG - chr3_4:39503648-39503667 Msa0499950:three_prime_UTR 40.0%
ACAATAGGGTTTGCAAAGAG+TGG - chr3_4:39503663-39503682 Msa0499950:three_prime_UTR 40.0%
CACTGTGAGGTTTGATAAAG+AGG + chr3_4:39503732-39503751 None:intergenic 40.0%
TTCTATAAAGGGACACTGTG+AGG + chr3_4:39503745-39503764 None:intergenic 40.0%
TGTCACCATCTAAATTGTGG+TGG - chr3_4:39504041-39504060 Msa0499950:three_prime_UTR 40.0%
CTTAGCCACCACAATTTAGA+TGG + chr3_4:39504049-39504068 None:intergenic 40.0%
AGTTAGGGAGTAAAAAAGGC+GGG + chr3_4:39504543-39504562 None:intergenic 40.0%
! TTGTGTGAGTTCTAACCACT+AGG + chr3_4:39504966-39504985 None:intergenic 40.0%
ACCAACTGAGTTAAACTCAC+GGG - chr3_4:39505054-39505073 Msa0499950:CDS 40.0%
CCTTCCCAAATTAAGAACGA+AGG + chr3_4:39505086-39505105 None:intergenic 40.0%
! CCTTCGTTCTTAATTTGGGA+AGG - chr3_4:39505083-39505102 Msa0499950:CDS 40.0%
!! TACTCTTGGTAATGGACCAA+TGG - chr3_4:39505369-39505388 Msa0499950:intron 40.0%
! TATGCTTTCATTTTCGGCTG+TGG - chr3_4:39505440-39505459 Msa0499950:intron 40.0%
! ATGCTTTCATTTTCGGCTGT+GGG - chr3_4:39505441-39505460 Msa0499950:intron 40.0%
CTTCTAACTTGGGAAAAGGT+TGG - chr3_4:39505714-39505733 Msa0499950:intron 40.0%
TAAGGCCATGCAGTTTGATT+TGG - chr3_4:39506450-39506469 Msa0499950:intron 40.0%
! ACTAAGGTGTAGTTAGCTTG+TGG + chr3_4:39506627-39506646 None:intergenic 40.0%
AAGCTAACTACACCTTAGTG+AGG - chr3_4:39506628-39506647 Msa0499950:intron 40.0%
AACTACACCTTAGTGAGGAT+TGG - chr3_4:39506633-39506652 Msa0499950:intron 40.0%
GGATAGAGATCTAACAGTGA+AGG + chr3_4:39506676-39506695 None:intergenic 40.0%
TTGTCTCATGGTTTGATGTG+TGG + chr3_4:39506971-39506990 None:intergenic 40.0%
CAATCTGCCAGAAATTGGAA+AGG - chr3_4:39507800-39507819 Msa0499950:intron 40.0%
CATTGAAAACATGGGCCAAA+TGG - chr3_4:39508176-39508195 Msa0499950:intron 40.0%
ATATTTCCAGCTCAAGGCAA+TGG - chr3_4:39508789-39508808 Msa0499950:intron 40.0%
TATTTCCAGCTCAAGGCAAT+GGG - chr3_4:39508790-39508809 Msa0499950:intron 40.0%
!! CCTTCACAAAAGTTTGCTGT+TGG + chr3_4:39508821-39508840 None:intergenic 40.0%
! CCAACAGCAAACTTTTGTGA+AGG - chr3_4:39508818-39508837 Msa0499950:intron 40.0%
GGATACATGGATCCTGATAA+TGG + chr3_4:39508854-39508873 None:intergenic 40.0%
TTATCAGGATCCATGTATCC+AGG - chr3_4:39508854-39508873 Msa0499950:intron 40.0%
CTCCATGTCCATAAACTGAT+GGG + chr3_4:39508988-39509007 None:intergenic 40.0%
TTTATGGACATGGAGGACAT+AGG - chr3_4:39508993-39509012 Msa0499950:intron 40.0%
TTATGGACATGGAGGACATA+GGG - chr3_4:39508994-39509013 Msa0499950:intron 40.0%
TGCTAGTGAGTCACATGAAC+AGG + chr3_4:39503495-39503514 None:intergenic 45.0%
TCGTGATTGAGGTTGTATCG+AGG + chr3_4:39503998-39504017 None:intergenic 45.0%
CGATACAACCTCAATCACGA+TGG - chr3_4:39503998-39504017 Msa0499950:three_prime_UTR 45.0%
TACAACCTCAATCACGATGG+TGG - chr3_4:39504001-39504020 Msa0499950:three_prime_UTR 45.0%
TTGCACACAACTCTGATGAG+AGG + chr3_4:39504819-39504838 None:intergenic 45.0%
CCAACTGAGTTAAACTCACG+GGG - chr3_4:39505055-39505074 Msa0499950:CDS 45.0%
TGTGGAGTTGCCATTATTCC+AGG - chr3_4:39505213-39505232 Msa0499950:intron 45.0%
GTGGAGTTGCCATTATTCCA+GGG - chr3_4:39505214-39505233 Msa0499950:intron 45.0%
! AGTGGCTATTGGCTAACCAT+TGG + chr3_4:39505388-39505407 None:intergenic 45.0%
! TTTCATTTTCGGCTGTGGGA+TGG - chr3_4:39505445-39505464 Msa0499950:intron 45.0%
ACCACCATAGGATGTATGTG+TGG - chr3_4:39506278-39506297 Msa0499950:CDS 45.0%
TCCACACATACATCCTATGG+TGG + chr3_4:39506282-39506301 None:intergenic 45.0%
GTGGACCAAATCAAACTGCA+TGG + chr3_4:39506458-39506477 None:intergenic 45.0%
! TAGCTTGTGGTACAGCTATG+TGG + chr3_4:39506614-39506633 None:intergenic 45.0%
GAGATCACCAATCCTCACTA+AGG + chr3_4:39506643-39506662 None:intergenic 45.0%
AGTTAGGGGACCAAATGTGA+GGG + chr3_4:39507959-39507978 None:intergenic 45.0%
AGAGGACCAGTTGCAGAATA+TGG - chr3_4:39508039-39508058 Msa0499950:intron 45.0%
GATGATCTGGAGAGATTCCT+TGG + chr3_4:39508243-39508262 None:intergenic 45.0%
ATTTCCAGCTCAAGGCAATG+GGG - chr3_4:39508791-39508810 Msa0499950:intron 45.0%
GATGGTCACCCATCAGTTTA+TGG - chr3_4:39508977-39508996 Msa0499950:intron 45.0%
CACCCATCAGTTTATGGACA+TGG - chr3_4:39508983-39509002 Msa0499950:intron 45.0%
CCTCCATGTCCATAAACTGA+TGG + chr3_4:39508989-39509008 None:intergenic 45.0%
CCATCAGTTTATGGACATGG+AGG - chr3_4:39508986-39509005 Msa0499950:intron 45.0%
TAGCTGGTGTTCCTGATACT+TGG - chr3_4:39509041-39509060 Msa0499950:CDS 45.0%
!!! AAAAATACTTATATATTTTT+TGG - chr3_4:39508000-39508019 Msa0499950:intron 5.0%
!!! CTATTTATTTTAAATTTTTT+GGG - chr3_4:39508498-39508517 Msa0499950:intron 5.0%
TGTCTCCACCATCGTGATTG+AGG + chr3_4:39504009-39504028 None:intergenic 50.0%
CCCCGTGAGTTTAACTCAGT+TGG + chr3_4:39505058-39505077 None:intergenic 50.0%
GAGTTAGGGGACCAAATGTG+AGG + chr3_4:39507960-39507979 None:intergenic 50.0%
TCAGGATCCATGTATCCAGG+AGG - chr3_4:39508857-39508876 Msa0499950:intron 50.0%
AACTGGACCTCCTGGATACA+TGG + chr3_4:39508867-39508886 None:intergenic 50.0%
AAAGCCAACTGTGCTGGAAC+TGG + chr3_4:39508884-39508903 None:intergenic 50.0%
!! CTCTCTAAAGCCAACTGTGC+TGG + chr3_4:39508890-39508909 None:intergenic 50.0%
ACATGGAGGACATAGGGACA+TGG - chr3_4:39509000-39509019 Msa0499950:intron 50.0%
GAACACCAGCTAGACACCAA+TGG + chr3_4:39509033-39509052 None:intergenic 50.0%
! ATGTGGAGAAGTGGGGTGTC+CGG - chr3_4:39504990-39505009 Msa0499950:CDS 55.0%
GTTCTCACGCAATGCGCTTC+TGG - chr3_4:39507727-39507746 Msa0499950:intron 55.0%
!! TGGCATTGGTGGCTTCACAC+AGG - chr3_4:39507848-39507867 Msa0499950:intron 55.0%
AGGTCCAGTTCCAGCACAGT+TGG - chr3_4:39508877-39508896 Msa0499950:intron 55.0%
! GGGACATGGATTGCAGCCAT+TGG - chr3_4:39509014-39509033 Msa0499950:CDS 55.0%
! TGCAGCCATTGGTGTCTAGC+TGG - chr3_4:39509025-39509044 Msa0499950:CDS 55.0%
! TGTGGAGAAGTGGGGTGTCC+GGG - chr3_4:39504991-39505010 Msa0499950:CDS 60.0%
GGTGTCCGGGGTTCAAACAC+CGG - chr3_4:39505004-39505023 Msa0499950:CDS 60.0%
AAGGGCCGGTGTTTGAACCC+CGG + chr3_4:39505012-39505031 None:intergenic 60.0%
CTCACGCAATGCGCTTCTGG+TGG - chr3_4:39507730-39507749 Msa0499950:intron 60.0%
GTCTCCCCATTGCCTTGAGC+TGG + chr3_4:39508798-39508817 None:intergenic 60.0%
TGTGCTGGAACTGGACCTCC+TGG + chr3_4:39508875-39508894 None:intergenic 60.0%
! GTGGAGAAGTGGGGTGTCCG+GGG - chr3_4:39504992-39505011 Msa0499950:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 39503434 39509428 39503434 ID=Msa0499950;Name=Msa0499950
chr3_4 mRNA 39503434 39509428 39503434 ID=Msa0499950-mRNA-1;Parent=Msa0499950;Name=Msa0499950-mRNA-1;_AED=0.07;_eAED=0.07;_QI=139|0.5|0.8|0.8|1|0.8|5|1010|238
chr3_4 exon 39503434 39504063 39503434 ID=Msa0499950-mRNA-1:exon:8119;Parent=Msa0499950-mRNA-1
chr3_4 exon 39504596 39505155 39504596 ID=Msa0499950-mRNA-1:exon:8118;Parent=Msa0499950-mRNA-1
chr3_4 exon 39506182 39506353 39506182 ID=Msa0499950-mRNA-1:exon:8117;Parent=Msa0499950-mRNA-1
chr3_4 exon 39507424 39507510 39507424 ID=Msa0499950-mRNA-1:exon:8116;Parent=Msa0499950-mRNA-1
chr3_4 exon 39509012 39509428 39509012 ID=Msa0499950-mRNA-1:exon:8115;Parent=Msa0499950-mRNA-1
chr3_4 five_prime_UTR 39509290 39509428 39509290 ID=Msa0499950-mRNA-1:five_prime_utr;Parent=Msa0499950-mRNA-1
chr3_4 CDS 39509012 39509289 39509012 ID=Msa0499950-mRNA-1:cds;Parent=Msa0499950-mRNA-1
chr3_4 CDS 39507424 39507510 39507424 ID=Msa0499950-mRNA-1:cds;Parent=Msa0499950-mRNA-1
chr3_4 CDS 39506182 39506353 39506182 ID=Msa0499950-mRNA-1:cds;Parent=Msa0499950-mRNA-1
chr3_4 CDS 39504976 39505155 39504976 ID=Msa0499950-mRNA-1:cds;Parent=Msa0499950-mRNA-1
chr3_4 three_prime_UTR 39504596 39504975 39504596 ID=Msa0499950-mRNA-1:three_prime_utr;Parent=Msa0499950-mRNA-1
chr3_4 three_prime_UTR 39503434 39504063 39503434 ID=Msa0499950-mRNA-1:three_prime_utr;Parent=Msa0499950-mRNA-1
Gene Sequence

>Msa0499950

ATGCTTATTGTGAGCAAAACCAGCATGAAATCTATCATCATATTTGTTTCTCTAATTCATGTTCTTCTCATGATTCATGTTCATGGGAAGACAATAGGGTTTGCAAAGAGTGGTTGTGATTTGTTTCAAGGGAAATGGGTATATGATGAATCATATCCTCTTTATCAAACCTCACAGTGTCCCTTTATAGAAAAAGAATTTGATTGTCAGAATAATGGTAGACCAGATAAATTCTATCTTAAGTATAGATGGCAGCCCACAAATTGTAACTTACCAAGACGTGTATGGAAAGAACTACTCTTGGTAATGGACCAATGGTTAGCCAATAGCCACTTAAATAAATCTCTATTATCTCAGAGAGATAAATTCAATGGTGAAGATTTCTTAAGAAGGCATAGAGGGAAAAGTATTTTGTTTGTTGGGGACTCTTTGAGTTTGAATCAATGGCAATCACTTACTTGCATGCTCCACATAGCTGTACCACAAGCTAACTACACCTTAGTGAGGATTGGTGATCTCTCCATTTTCACCTTCACTACTTATGGTGTTAAGGTGATGTTCTCACGCAATGCGCTTCTGGTGGACATTGTTAGTGAAAATATTGGTAGGGTCCTGAAATTAGACTCTATTCAATCTGCCAGAAATTGGAAAGGAATTGATGTGTTGATATTTGATTCTTGGCATTGGTGGCTTCACACAGGAAGAAAACAACCGTAA
Protein sequence

>Msa0499950

MLIVSKTSMKSIIIFVSLIHVLLMIHVHGKTIGFAKSGCDLFQGKWVYDESYPLYQTSQCPFIEKEFDCQNNGRPDKFYLKYRWQPTNCNLPRRVWKELLLVMDQWLANSHLNKSLLSQRDKFNGEDFLRRHRGKSILFVGDSLSLNQWQSLTCMLHIAVPQANYTLVRIGDLSIFTFTTYGVKVMFSRNALLVDIVSENIGRVLKLDSIQSARNWKGIDVLIFDSWHWWLHTGRKQP*