AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1356230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1356230 MtrunA17_Chr3g0120521 84.270 89 14 0 1 89 506 594 1.71e-46 158
Msa1356230 MtrunA17_Chr7g0261701 62.366 93 35 0 1 93 29 121 1.36e-39 133
Msa1356230 MtrunA17_Chr4g0076611 61.290 93 36 0 1 93 433 525 1.42e-36 130
Msa1356230 MtrunA17_Chr4g0057531 61.290 93 36 0 1 93 510 602 1.57e-35 127
Msa1356230 MtrunA17_Chr4g0057511 60.215 93 37 0 1 93 349 441 1.07e-34 124
Msa1356230 MtrunA17_Chr4g0057481 61.290 93 36 0 1 93 423 515 2.97e-34 124
Msa1356230 MtrunA17_Chr5g0447171 66.667 90 21 1 1 90 207 287 4.58e-34 122
Msa1356230 MtrunA17_Chr4g0057491 58.065 93 39 0 1 93 355 447 1.81e-33 121
Msa1356230 MtrunA17_Chr5g0439271 64.045 89 32 0 1 89 337 425 4.73e-33 120
Msa1356230 MtrunA17_Chr4g0057461 58.427 89 37 0 1 89 361 449 5.31e-33 120
Msa1356230 MtrunA17_Chr4g0057471 58.065 93 39 0 1 93 442 534 1.89e-32 119
Msa1356230 MtrunA17_Chr8g0344331 61.798 89 34 0 1 89 502 590 2.67e-32 118
Msa1356230 MtrunA17_Chr7g0250481 62.222 90 33 1 1 89 531 620 5.13e-32 117
Msa1356230 MtrunA17_Chr4g0053781 53.933 89 41 0 1 89 484 572 1.23e-31 116
Msa1356230 MtrunA17_Chr7g0250441 57.447 94 39 1 1 93 16 109 1.41e-31 112
Msa1356230 MtrunA17_Chr5g0401511 55.056 89 40 0 1 89 490 578 6.50e-31 114
Msa1356230 MtrunA17_Chr7g0250371 60.000 90 35 1 1 89 518 607 8.49e-31 114
Msa1356230 MtrunA17_Chr5g0423261 55.914 93 41 0 1 93 355 447 1.80e-30 113
Msa1356230 MtrunA17_Chr7g0250331 60.000 90 35 1 1 89 539 628 2.23e-30 112
Msa1356230 MtrunA17_Chr4g0020651 52.874 87 41 0 1 87 486 572 3.14e-30 112
Msa1356230 MtrunA17_Chr4g0037221 52.809 89 42 0 1 89 492 580 5.47e-30 111
Msa1356230 MtrunA17_Chr7g0250411 60.000 90 35 1 1 89 517 606 7.94e-30 111
Msa1356230 MtrunA17_Chr1g0204321 57.303 89 38 0 1 89 507 595 9.53e-30 111
Msa1356230 MtrunA17_Chr1g0152771 53.933 89 41 0 1 89 482 570 1.03e-29 110
Msa1356230 MtrunA17_Chr7g0250391 57.778 90 37 1 1 89 516 605 1.55e-29 110
Msa1356230 MtrunA17_Chr2g0300751 55.056 89 40 0 1 89 502 590 2.18e-29 110
Msa1356230 MtrunA17_Chr7g0227991 52.747 91 43 0 1 91 471 561 3.28e-29 109
Msa1356230 MtrunA17_Chr2g0285111 53.261 92 42 1 1 91 519 610 7.34e-29 108
Msa1356230 MtrunA17_Chr6g0470851 50.549 91 43 1 1 89 466 556 7.34e-29 108
Msa1356230 MtrunA17_Chr3g0134621 52.809 89 42 0 1 89 459 547 8.02e-29 108
Msa1356230 MtrunA17_Chr1g0146251 52.326 86 41 0 4 89 476 561 9.47e-29 108
Msa1356230 MtrunA17_Chr1g0170081 53.488 86 40 0 4 89 472 557 1.03e-28 108
Msa1356230 MtrunA17_Chr2g0285121 54.348 92 41 1 1 91 516 607 1.16e-28 108
Msa1356230 MtrunA17_Chr4g0070391 54.444 90 40 1 1 90 481 569 1.25e-28 107
Msa1356230 MtrunA17_Chr8g0376841 53.261 92 43 0 1 92 493 584 2.30e-28 107
Msa1356230 MtrunA17_Chr8g0344971 52.874 87 41 0 3 89 485 571 2.82e-28 107
Msa1356230 MtrunA17_Chr1g0146271 51.163 86 42 0 4 89 474 559 4.27e-28 106
Msa1356230 MtrunA17_Chr8g0334521 48.352 91 47 0 1 91 471 561 7.21e-28 105
Msa1356230 MtrunA17_Chr6g0470881 52.273 88 40 1 4 89 469 556 7.64e-28 105
Msa1356230 MtrunA17_Chr7g0262161 55.056 89 40 0 1 89 485 573 7.75e-28 105
Msa1356230 MtrunA17_Chr7g0246461 50.562 89 44 0 1 89 467 555 2.79e-27 103
Msa1356230 MtrunA17_Chr4g0035881 46.602 103 51 1 1 99 225 327 1.16e-26 100
Msa1356230 MtrunA17_Chr5g0407701 54.545 88 32 1 2 89 494 573 1.35e-26 102
Msa1356230 MtrunA17_Chr6g0480721 50.538 93 44 2 1 92 340 431 1.52e-26 102
Msa1356230 MtrunA17_Chr2g0301011 49.438 89 45 0 1 89 335 423 2.70e-26 100
Msa1356230 MtrunA17_Chr8g0346361 48.352 91 47 0 1 91 234 324 3.03e-26 100
Msa1356230 MtrunA17_Chr8g0351011 50.549 91 45 0 1 91 489 579 4.54e-26 100
Msa1356230 MtrunA17_Chr8g0334491 52.273 88 32 1 4 91 476 553 1.00e-25 99.8
Msa1356230 MtrunA17_Chr8g0369891 53.933 89 31 2 1 89 498 576 8.45e-25 97.1
Msa1356230 MtrunA17_Chr4g0026641 51.136 88 41 1 2 89 491 576 9.32e-25 97.1
Msa1356230 MtrunA17_Chr5g0398331 53.933 89 34 1 1 89 489 570 3.89e-24 95.1
Msa1356230 MtrunA17_Chr4g0057061 53.933 89 37 2 1 89 512 596 1.14e-23 94.0
Msa1356230 MtrunA17_Chr8g0377961 48.913 92 40 2 1 92 515 599 1.34e-23 93.6
Msa1356230 MtrunA17_Chr5g0398241 53.409 88 34 1 4 91 495 575 1.37e-23 93.6
Msa1356230 MtrunA17_Chr5g0398401 53.409 88 34 1 4 91 495 575 1.40e-23 93.6
Msa1356230 MtrunA17_Chr5g0398351 53.488 86 33 1 4 89 475 553 2.12e-23 93.2
Msa1356230 MtrunA17_Chr5g0398351 52.326 86 34 1 4 89 1246 1324 7.86e-23 91.7
Msa1356230 MtrunA17_Chr3g0079971 47.126 87 44 2 5 91 473 557 5.45e-23 92.0
Msa1356230 MtrunA17_Chr3g0143231 47.778 90 46 1 1 90 2 90 5.69e-23 84.7
Msa1356230 MtrunA17_Chr7g0246451 44.444 90 50 0 2 91 483 572 7.24e-23 91.7
Msa1356230 MtrunA17_Chr5g0398311 51.685 89 36 1 1 89 485 566 9.50e-23 91.3
Msa1356230 MtrunA17_Chr1g0189251 50.549 91 35 3 4 89 486 571 1.17e-22 90.9
Msa1356230 MtrunA17_Chr8g0345991 48.913 92 37 3 3 89 484 570 2.54e-22 90.1
Msa1356230 MtrunA17_Chr2g0312861 48.000 75 39 0 17 91 1 75 7.56e-22 87.0
Msa1356230 MtrunA17_Chr2g0303881 48.352 91 38 2 3 89 458 543 1.10e-21 88.2
Msa1356230 MtrunA17_Chr7g0266051 47.191 89 47 0 1 89 442 530 1.28e-21 87.8
Msa1356230 MtrunA17_Chr2g0301031 48.649 74 38 0 17 90 1 74 1.66e-21 85.1
Msa1356230 MtrunA17_Chr3g0136151 46.739 92 48 1 1 92 490 580 2.42e-21 87.0
Msa1356230 MtrunA17_Chr1g0189561 51.064 94 39 2 2 91 487 577 2.75e-21 87.0
Msa1356230 MtrunA17_Chr8g0354741 47.191 89 45 1 1 89 368 454 2.92e-21 87.0
Msa1356230 MtrunA17_Chr3g0105451 45.652 92 37 1 1 92 19 97 8.02e-21 84.0
Msa1356230 MtrunA17_Chr8g0345971 47.872 94 33 3 4 89 490 575 8.07e-21 85.9
Msa1356230 MtrunA17_Chr5g0418921 46.739 92 39 2 3 89 18 104 3.64e-20 80.1
Msa1356230 MtrunA17_Chr3g0143251 45.055 91 49 1 2 92 495 584 5.64e-20 83.2
Msa1356230 MtrunA17_Chr8g0345981 43.269 104 44 4 3 96 488 586 6.40e-20 83.2
Msa1356230 MtrunA17_Chr8g0354771 42.222 90 51 1 1 89 495 584 1.38e-19 82.0
Msa1356230 MtrunA17_Chr7g0248871 46.739 92 46 2 2 91 493 583 2.43e-19 81.6
Msa1356230 MtrunA17_Chr4g0071191 42.222 90 50 2 4 92 63 151 6.46e-19 78.6
Msa1356230 MtrunA17_Chr7g0276151 45.455 88 45 2 4 89 486 572 1.19e-18 79.3
Msa1356230 MtrunA17_Chr4g0026661 45.205 73 38 1 17 89 12 82 1.44e-18 77.8
Msa1356230 MtrunA17_Chr4g0071871 45.745 94 43 2 1 89 542 632 3.82e-18 78.2
Msa1356230 MtrunA17_Chr7g0248861 46.067 89 47 1 2 89 495 583 5.54e-18 77.8
Msa1356230 MtrunA17_Chr3g0142971 42.353 85 48 1 1 85 2 85 2.74e-17 70.5
Msa1356230 MtrunA17_Chr4g0075331 43.750 96 42 3 4 92 471 561 1.27e-16 73.6
Msa1356230 MtrunA17_Chr8g0345961 48.052 77 39 1 13 89 498 573 7.25e-16 71.6
Msa1356230 MtrunA17_Chr2g0298601 42.391 92 41 3 1 85 542 628 9.38e-16 71.2
Msa1356230 MtrunA17_Chr8g0380961 36.842 95 46 3 4 90 576 664 3.94e-14 66.6
Msa1356230 MtrunA17_Chr2g0302951 36.082 97 48 3 4 92 568 658 2.27e-13 64.3
Msa1356230 MtrunA17_Chr3g0097061 43.396 53 30 0 39 91 321 373 2.85e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1356230 AT2G04160.1 74.157 89 23 0 1 89 505 593 5.82e-39 137
Msa1356230 AT5G59810.1 68.817 93 29 0 1 93 511 603 6.09e-38 134
Msa1356230 AT2G05920.1 59.551 89 36 0 1 89 480 568 1.82e-33 122
Msa1356230 AT5G58840.3 51.000 100 49 0 1 100 443 542 7.04e-33 119
Msa1356230 AT5G67360.1 57.609 92 39 0 1 92 486 577 8.73e-33 120
Msa1356230 AT3G14067.1 58.427 89 37 0 1 89 490 578 3.90e-31 115
Msa1356230 AT5G59090.2 54.839 93 40 1 1 93 465 555 6.44e-31 114
Msa1356230 AT5G59100.1 53.933 89 41 0 1 89 472 560 6.53e-31 114
Msa1356230 AT5G58840.1 53.933 89 41 0 1 89 443 531 1.04e-30 114
Msa1356230 AT5G58820.1 52.809 89 42 0 1 89 439 527 3.96e-30 112
Msa1356230 AT5G59090.3 56.180 89 37 1 1 89 463 549 4.22e-30 112
Msa1356230 AT5G59090.1 56.180 89 37 1 1 89 465 551 4.34e-30 112
Msa1356230 AT5G58830.1 51.685 89 43 0 1 89 442 530 1.70e-29 110
Msa1356230 AT1G04110.1 56.180 89 39 0 1 89 496 584 2.92e-29 110
Msa1356230 AT5G59120.1 55.056 89 38 1 1 89 464 550 4.75e-29 109
Msa1356230 AT5G51750.1 56.180 89 39 0 1 89 504 592 5.65e-29 109
Msa1356230 AT4G34980.1 55.056 89 40 0 1 89 482 570 1.25e-28 108
Msa1356230 AT3G14240.1 52.222 90 43 0 1 90 493 582 6.59e-28 106
Msa1356230 AT3G46840.1 51.685 89 41 1 1 89 473 559 5.51e-27 103
Msa1356230 AT5G45650.2 50.562 89 44 0 1 89 522 610 1.16e-26 102
Msa1356230 AT5G45650.1 50.562 89 44 0 1 89 522 610 1.16e-26 102
Msa1356230 AT1G01900.1 51.648 91 44 0 1 91 496 586 1.33e-26 102
Msa1356230 AT4G26330.2 48.515 101 51 1 1 100 450 550 7.23e-26 100
Msa1356230 AT4G26330.1 48.515 101 51 1 1 100 464 564 7.24e-26 100
Msa1356230 AT3G46850.1 48.315 89 44 1 1 89 473 559 2.87e-25 98.6
Msa1356230 AT5G03620.2 50.000 88 44 0 4 91 490 577 4.77e-25 98.2
Msa1356230 AT5G59190.1 44.792 96 49 2 4 96 430 524 1.25e-24 97.1
Msa1356230 AT4G00230.2 46.237 93 50 0 1 93 480 572 1.33e-24 96.7
Msa1356230 AT4G10540.1 56.180 89 32 2 1 89 504 585 2.78e-24 95.9
Msa1356230 AT5G03620.1 50.000 86 43 0 4 89 490 575 4.13e-24 95.5
Msa1356230 AT4G10550.1 54.348 92 34 2 1 92 508 591 4.93e-24 95.1
Msa1356230 AT4G00230.1 47.253 91 48 0 1 91 480 570 5.21e-24 95.1
Msa1356230 AT4G10550.4 54.348 92 34 2 1 92 452 535 6.49e-24 94.7
Msa1356230 AT4G10550.2 54.348 92 34 2 1 92 452 535 6.49e-24 94.7
Msa1356230 AT4G10550.3 54.348 92 34 2 1 92 524 607 6.67e-24 94.7
Msa1356230 AT4G21650.1 55.056 89 33 2 1 89 500 581 7.19e-24 94.7
Msa1356230 AT5G59130.4 51.163 86 39 1 4 89 468 550 4.16e-23 92.4
Msa1356230 AT5G59130.1 51.163 86 39 1 4 89 464 546 4.24e-23 92.4
Msa1356230 AT5G59130.2 51.163 86 39 1 4 89 458 540 4.76e-23 92.4
Msa1356230 AT5G59130.3 51.163 86 39 1 4 89 429 511 5.03e-23 92.4
Msa1356230 AT5G67090.1 49.438 89 45 0 1 89 473 561 1.14e-22 91.3
Msa1356230 AT1G20160.1 48.315 89 45 1 1 89 491 578 1.23e-22 91.3
Msa1356230 AT1G20160.2 48.315 89 45 1 1 89 452 539 1.25e-22 91.3
Msa1356230 AT1G32950.1 53.933 89 34 2 1 89 502 583 2.18e-22 90.5
Msa1356230 AT1G32950.2 53.933 89 34 2 1 89 451 532 2.53e-22 90.5
Msa1356230 AT4G10520.1 48.913 92 40 2 1 92 485 569 3.24e-22 90.1
Msa1356230 AT4G21630.1 52.809 89 35 2 1 89 493 574 4.47e-22 89.7
Msa1356230 AT1G32970.1 50.000 92 39 2 1 92 463 547 5.20e-22 89.4
Msa1356230 AT4G10510.1 55.056 89 33 2 1 89 494 575 1.03e-21 88.6
Msa1356230 AT4G10510.4 55.056 89 33 2 1 89 450 531 1.16e-21 88.6
Msa1356230 AT4G10510.3 55.056 89 33 2 1 89 506 587 1.21e-21 88.6
Msa1356230 AT4G10510.2 55.056 89 33 2 1 89 435 516 1.26e-21 88.6
Msa1356230 AT5G11940.1 48.315 89 39 1 1 89 491 572 1.79e-21 87.8
Msa1356230 AT4G21640.1 52.809 89 35 2 1 89 467 548 5.80e-21 86.7
Msa1356230 AT1G20150.1 52.326 86 40 1 4 89 498 582 8.93e-21 85.9
Msa1356230 AT1G32960.1 52.326 86 34 2 4 89 509 587 1.51e-20 85.5
Msa1356230 AT1G66220.1 47.191 89 33 1 4 92 495 569 1.55e-20 85.1
Msa1356230 AT4G21326.1 48.315 89 39 1 4 92 431 512 9.49e-20 83.2
Msa1356230 AT1G66210.1 50.562 89 32 2 1 89 495 571 2.43e-19 82.0
Msa1356230 AT2G39850.1 43.434 99 45 4 1 92 491 585 2.55e-19 81.6
Msa1356230 AT5G45640.1 47.222 72 38 0 19 90 502 573 3.31e-19 81.6
Msa1356230 AT2G19170.1 47.368 95 40 3 1 89 538 628 6.26e-19 80.9
Msa1356230 AT2G19170.2 47.368 95 40 3 1 89 538 628 6.26e-19 80.9
Msa1356230 AT1G32940.3 51.111 90 37 1 1 90 503 585 6.36e-19 80.5
Msa1356230 AT4G30020.3 45.745 94 43 2 1 89 539 629 1.35e-18 79.7
Msa1356230 AT4G30020.1 45.745 94 43 2 1 89 539 629 1.35e-18 79.7
Msa1356230 AT4G30020.4 45.745 94 43 2 1 89 539 629 1.35e-18 79.7
Msa1356230 AT4G30020.2 45.745 94 43 2 1 89 539 629 1.35e-18 79.7
Msa1356230 AT1G32940.1 50.562 89 37 1 1 89 503 584 4.86e-18 78.2
Msa1356230 AT1G32940.4 50.562 89 37 1 1 89 452 533 5.43e-18 78.2
Msa1356230 AT1G32940.2 50.562 89 37 1 1 89 452 533 5.43e-18 78.2
Msa1356230 AT4G21323.1 43.182 88 41 1 5 92 537 615 3.95e-16 72.8
Msa1356230 AT1G62340.1 43.750 96 42 4 4 92 563 653 1.12e-14 68.6
Msa1356230 AT4G15040.2 37.931 87 53 1 4 89 426 512 1.32e-14 68.6
Msa1356230 AT4G15040.1 37.931 87 53 1 4 89 426 512 1.32e-14 68.6
Msa1356230 AT1G30600.1 38.462 91 48 2 4 89 554 641 1.09e-13 65.9
Msa1356230 AT5G44530.2 35.106 94 47 3 4 89 564 651 4.81e-13 63.9
Msa1356230 AT5G44530.1 35.106 94 47 3 4 89 564 651 5.24e-13 63.9
Msa1356230 AT4G10530.1 38.043 92 37 1 1 92 489 560 1.84e-12 62.4
Msa1356230 AT4G20430.3 35.165 91 51 2 4 89 579 666 7.47e-12 60.5
Msa1356230 AT4G20430.1 35.165 91 51 2 4 89 579 666 7.47e-12 60.5
Msa1356230 AT4G20430.2 35.165 91 51 2 4 89 555 642 7.83e-12 60.5

Find 103 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGCAAAATTTGATAATTT+TGG 0.086768 tig0015546:-3342 Msa1356230:five_prime_UTR
GCATCTGTTGCTTTAATAAT+TGG 0.089925 tig0015546:+2599 None:intergenic
TAAAGCAACAGATGCTAAAT+TGG 0.221704 tig0015546:-2591 Msa1356230:five_prime_UTR
TGAGAACCTTGTATCCTTCT+TGG 0.224032 tig0015546:-1758 Msa1356230:CDS
ATTGTTGGTTATTTGGAAAT+TGG 0.236564 tig0015546:-3120 Msa1356230:five_prime_UTR
TTCACTAGTTATGTTCTTCT+TGG 0.240053 tig0015546:-2851 Msa1356230:five_prime_UTR
GTTGTTGTTGCTTGCTCTGC+TGG 0.279959 tig0015546:-2956 Msa1356230:five_prime_UTR
ATCTCGGGTACAATGGTATT+TGG 0.284686 tig0015546:+2033 None:intergenic
CCTCACATTTCGGGAATTGC+AGG 0.302985 tig0015546:-1786 Msa1356230:CDS
TGACTCTCAATTATCTTGTT+TGG 0.307210 tig0015546:-2675 Msa1356230:five_prime_UTR
TGTGATCCAGAGTCCCATTC+TGG 0.311076 tig0015546:+2439 None:intergenic
AGTCTGAAGTTGAGATTTCT+TGG 0.315236 tig0015546:+1648 None:intergenic
GGTTATTTGGAAATTGGTTT+TGG 0.327450 tig0015546:-3114 Msa1356230:five_prime_UTR
TACAATGGTATTTGGCCCTT+TGG 0.331318 tig0015546:+2041 None:intergenic
AAATCATTTCATAAATATTC+CGG 0.336856 tig0015546:-2997 Msa1356230:five_prime_UTR
ATGTCAAGCCCTCACATTTC+GGG 0.340640 tig0015546:-1795 Msa1356230:CDS
CATGTCAAGCCCTCACATTT+CGG 0.341153 tig0015546:-1796 Msa1356230:CDS
ATTTCCACTATTGTTATCAT+TGG 0.342216 tig0015546:+2323 None:intergenic
GCTTGCTCTGCTGGCAATGA+TGG 0.343896 tig0015546:-2947 Msa1356230:five_prime_UTR
CCTTATCCACTCCTCCATTC+AGG 0.352074 tig0015546:+2388 None:intergenic
TTTCTATCAACCAATGTGAT+TGG 0.355031 tig0015546:+3170 None:intergenic
GGAAGAACAGAAAGCCAATT+GGG 0.382375 tig0015546:+3218 None:intergenic
CATCTGTTGCTTTAATAATT+GGG 0.387808 tig0015546:+2600 None:intergenic
TTACTGTAATTTCTATCAAT+AGG 0.388983 tig0015546:-2749 Msa1356230:five_prime_UTR
TTGTCGATCTCTTCATTAGT+TGG 0.405128 tig0015546:+1855 None:intergenic
ACACCTGGTGCTGTGATATC+AGG 0.406349 tig0015546:+1912 None:intergenic
ACCCAGAAAAGCTCAGTTCA+TGG 0.406753 tig0015546:-3361 Msa1356230:five_prime_UTR
TGGCAACGGTTTAAGCTTCA+AGG 0.407648 tig0015546:-2831 Msa1356230:five_prime_UTR
CTTGCCAATGATAACAATAG+TGG 0.413339 tig0015546:-2327 Msa1356230:five_prime_UTR
TTTGATGCAGTAGCCTCATT+TGG 0.413957 tig0015546:+2923 None:intergenic
TCTTGTTTGGTTCAATCAAC+GGG 0.414439 tig0015546:-2662 Msa1356230:five_prime_UTR
AATATATCATCAAAAGAAGT+TGG 0.416216 tig0015546:+2785 None:intergenic
TGATCAAGAATGAGATTTGT+GGG 0.416911 tig0015546:+3418 None:intergenic
TTCTATCAACCAATGTGATT+GGG 0.424736 tig0015546:+3171 None:intergenic
TCAAAGAGAAGTTGATCAAA+TGG 0.426603 tig0015546:-1626 Msa1356230:three_prime_UTR
CAACAACAATATTTGTAAAC+CGG 0.431164 tig0015546:+2978 None:intergenic
GAGAAATTTATGTTAGAAGC+AGG 0.435055 tig0015546:+2273 None:intergenic
TGCAGCGAGCTTTGCCAGAA+TGG 0.435817 tig0015546:-2453 Msa1356230:intron
CTTGTATCCTTCTTGGAGTC+CGG 0.441288 tig0015546:-1751 Msa1356230:CDS
GAATGAGATTTGTGGGATTG+AGG 0.442758 tig0015546:+3425 None:intergenic
TGAGTAAGACAAATTGAATT+CGG 0.461464 tig0015546:+1829 None:intergenic
GTCTGAAGTTGAGATTTCTT+GGG 0.461732 tig0015546:+1649 None:intergenic
GATTAACACCTTTAGAATCT+CGG 0.462819 tig0015546:+2017 None:intergenic
ATCTTGTTTGGTTCAATCAA+CGG 0.466097 tig0015546:-2663 Msa1356230:five_prime_UTR
AATTAGAATAGTTTGAAAAG+TGG 0.469091 tig0015546:+1592 None:intergenic
AGGAAGAACAGAAAGCCAAT+TGG 0.469166 tig0015546:+3217 None:intergenic
TTTGTGGGATTGAGGAATTG+AGG 0.470007 tig0015546:+3433 None:intergenic
CCTGAATGGAGGAGTGGATA+AGG 0.471847 tig0015546:-2388 Msa1356230:five_prime_UTR
GAATTGGTTGTTGGAGATGA+AGG 0.478589 tig0015546:-3092 Msa1356230:five_prime_UTR
TCGAAAAGAGAAGAATGAAA+AGG 0.479177 tig0015546:-3064 Msa1356230:five_prime_UTR
TTGATCAAGAATGAGATTTG+TGG 0.480475 tig0015546:+3417 None:intergenic
AGGAGTGGATAAGGGAGAAC+AGG 0.483171 tig0015546:-2379 Msa1356230:five_prime_UTR
AAAATAGTAGTGTGCCTGAA+TGG 0.485193 tig0015546:-2402 Msa1356230:five_prime_UTR
GCAGCGAGCTTTGCCAGAAT+GGG 0.489122 tig0015546:-2452 Msa1356230:intron
ATTAACACCTTTAGAATCTC+GGG 0.499143 tig0015546:+2018 None:intergenic
GGATGAAAATATTGCCATGA+TGG 0.505910 tig0015546:+2062 None:intergenic
CACTATTGTTATCATTGGCA+AGG 0.506923 tig0015546:+2328 None:intergenic
AAATTGAGATACCTACCAAA+TGG 0.508336 tig0015546:-3485 Msa1356230:five_prime_UTR
CAGCCTGATATCACAGCACC+AGG 0.512049 tig0015546:-1915 Msa1356230:CDS
TCTAACATAAATTTCTCTGA+TGG 0.516359 tig0015546:-2267 Msa1356230:five_prime_UTR
CTGCATCAAATCTAGCTCCT+TGG 0.516497 tig0015546:-2910 Msa1356230:five_prime_UTR
CTGAATGGAGGAGTGGATAA+GGG 0.525356 tig0015546:-2387 Msa1356230:five_prime_UTR
GAACTCTCTATCCATTGTGC+TGG 0.525429 tig0015546:+2871 None:intergenic
AGTTATGTTCTTCTTGGCAA+CGG 0.530911 tig0015546:-2845 Msa1356230:five_prime_UTR
AAGGATACAAGGTTCTCAAA+AGG 0.532636 tig0015546:+1763 None:intergenic
GGAGAACAGGCTCTTATAGC+TGG 0.534606 tig0015546:-2366 Msa1356230:five_prime_UTR
ATTCAATTTGTCTTACTCAC+CGG 0.545243 tig0015546:-1825 Msa1356230:CDS
GCTTTGCCAGAATGGGACTC+TGG 0.545631 tig0015546:-2445 Msa1356230:five_prime_UTR
CATTGTACCCGAGATTCTAA+AGG 0.548172 tig0015546:-2025 Msa1356230:intron
GCTCTTATAGCTGGTGCTGT+AGG 0.550029 tig0015546:-2357 Msa1356230:five_prime_UTR
AGAATAGTTTGAAAAGTGGA+AGG 0.552146 tig0015546:+1596 None:intergenic
TGGCTCTTCAGCTTCAGTGA+AGG 0.552957 tig0015546:+1875 None:intergenic
GCTCCTTGGTATATCACAGT+TGG 0.557076 tig0015546:-2896 Msa1356230:five_prime_UTR
GATGAAAATATTGCCATGAT+GGG 0.560543 tig0015546:+2063 None:intergenic
ATTGTTATTCCCAATCACAT+TGG 0.561112 tig0015546:-3180 Msa1356230:five_prime_UTR
TATAGCTGGTGCTGTAGGAG+TGG 0.566698 tig0015546:-2352 Msa1356230:five_prime_UTR
ATAGCAGATTTAATAGCAGC+CGG 0.569981 tig0015546:+1732 None:intergenic
CAAGAGTTTCTTCTTACCTG+TGG 0.573388 tig0015546:+1704 None:intergenic
CGGACTCCAAGAAGGATACA+AGG 0.588458 tig0015546:+1752 None:intergenic
CTTTAGAATCTCGGGTACAA+TGG 0.591821 tig0015546:+2026 None:intergenic
AAATCTGCTATCATGACCAC+AGG 0.593204 tig0015546:-1720 Msa1356230:CDS
CGAAATGTGAGGGCTTGACA+TGG 0.595430 tig0015546:+1797 None:intergenic
AGGGCTTGACATGGATGTGC+CGG 0.596264 tig0015546:+1806 None:intergenic
CTGCAATTCCCGAAATGTGA+GGG 0.596326 tig0015546:+1787 None:intergenic
TGGCAATGATGGACCAAATG+AGG 0.596925 tig0015546:-2936 Msa1356230:five_prime_UTR
CACAGTTGGTGCCAGCACAA+TGG 0.602894 tig0015546:-2882 Msa1356230:five_prime_UTR
ATAGCAGCCGGACTCCAAGA+AGG 0.603386 tig0015546:+1744 None:intergenic
TCTGGATCACAAGAAGGTGA+AGG 0.614201 tig0015546:-2427 Msa1356230:five_prime_UTR
TGGGACTCTGGATCACAAGA+AGG 0.633879 tig0015546:-2433 Msa1356230:five_prime_UTR
CTGGATCACAAGAAGGTGAA+GGG 0.633941 tig0015546:-2426 Msa1356230:five_prime_UTR
GCTGCTATAACCGACACACC+TGG 0.638678 tig0015546:+1897 None:intergenic
GAAGAACAGAAAGCCAATTG+GGG 0.645538 tig0015546:+3219 None:intergenic
TTAGAAGCAGGAAGAACATG+AGG 0.651996 tig0015546:+2285 None:intergenic
ATAGTAGTGTGCCTGAATGG+AGG 0.653182 tig0015546:-2399 Msa1356230:five_prime_UTR
GGATGTGTAATATAAGCCAC+TGG 0.654484 tig0015546:+2114 None:intergenic
CTTGTTTGGTTCAATCAACG+GGG 0.655523 tig0015546:-2661 Msa1356230:five_prime_UTR
TATCACAGCACCAGGTGTGT+CGG 0.657844 tig0015546:-1907 Msa1356230:CDS
TGAAAATATTGCCATGATGG+GGG 0.660947 tig0015546:+2065 None:intergenic
CCTGCAATTCCCGAAATGTG+AGG 0.663023 tig0015546:+1786 None:intergenic
ATGAAAATATTGCCATGATG+GGG 0.674957 tig0015546:+2064 None:intergenic
AGTGTGCCTGAATGGAGGAG+TGG 0.692602 tig0015546:-2394 Msa1356230:five_prime_UTR
GCACCAACTGTGATATACCA+AGG 0.694138 tig0015546:+2893 None:intergenic
TTGTTTGGTTCAATCAACGG+GGG 0.709692 tig0015546:-2660 Msa1356230:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGTTTTTCAATTAAAAAT+TGG - tig0015546:1755-1774 Msa1356230:CDS 10.0%
!!! ATTTTTAATTGAAAACTAAA+AGG + tig0015546:1782-1801 None:intergenic 10.0%
!!! TTTTAGTTTTCAATTAAAAA+TGG - tig0015546:1781-1800 Msa1356230:CDS 10.0%
!! TTATTCATTACTAAAAAATT+TGG + tig0015546:1911-1930 None:intergenic 10.0%
!!! ACTAATGATATTATATTTTT+TGG + tig0015546:2519-2538 None:intergenic 10.0%
!!! TTAGTTTTTCAATTAAAAAT+TGG - tig0015546:1755-1774 Msa1356230:CDS 10.0%
!!! ATTTTTAATTGAAAACTAAA+AGG + tig0015546:1782-1801 None:intergenic 10.0%
!!! TTTTAGTTTTCAATTAAAAA+TGG - tig0015546:1781-1800 Msa1356230:CDS 10.0%
!! TTATTCATTACTAAAAAATT+TGG + tig0015546:1911-1930 None:intergenic 10.0%
!!! ACTAATGATATTATATTTTT+TGG + tig0015546:2519-2538 None:intergenic 10.0%
!!! TTTTCAATTAAAAATGGATT+AGG - tig0015546:1787-1806 Msa1356230:CDS 15.0%
!! GTAATGAATAAAAAAATTGT+TGG - tig0015546:1920-1939 Msa1356230:intron 15.0%
!!! TAAAAAAATTGTTGGTTATT+TGG - tig0015546:1928-1947 Msa1356230:intron 15.0%
!! AAATCATTTCATAAATATTC+CGG - tig0015546:2058-2077 Msa1356230:CDS 15.0%
!!! TTTTCAATTAAAAATGGATT+AGG - tig0015546:1787-1806 Msa1356230:CDS 15.0%
!! GTAATGAATAAAAAAATTGT+TGG - tig0015546:1920-1939 Msa1356230:intron 15.0%
!!! TAAAAAAATTGTTGGTTATT+TGG - tig0015546:1928-1947 Msa1356230:intron 15.0%
!! AAATCATTTCATAAATATTC+CGG - tig0015546:2058-2077 Msa1356230:CDS 15.0%
!!! ATCTCAATTTTAATTTTTCC+CGG + tig0015546:1560-1579 None:intergenic 20.0%
!!! GAATTTTTATGTTATCCATT+TGG + tig0015546:1588-1607 None:intergenic 20.0%
!!! ATGGCAAAATTTGATAATTT+TGG - tig0015546:1713-1732 Msa1356230:CDS 20.0%
!!! TTGATAATTTTGGATATAGA+AGG - tig0015546:1723-1742 Msa1356230:CDS 20.0%
!! AACAATATGTAAGAGAAAAA+AGG + tig0015546:1861-1880 None:intergenic 20.0%
!! AATATATCATCAAAAGAAGT+TGG + tig0015546:2273-2292 None:intergenic 20.0%
!! TTACTGTAATTTCTATCAAT+AGG - tig0015546:2306-2325 Msa1356230:five_prime_UTR 20.0%
!! AATTAGAATAGTTTGAAAAG+TGG + tig0015546:3466-3485 None:intergenic 20.0%
!!! ATCTCAATTTTAATTTTTCC+CGG + tig0015546:1560-1579 None:intergenic 20.0%
!!! GAATTTTTATGTTATCCATT+TGG + tig0015546:1588-1607 None:intergenic 20.0%
!!! ATGGCAAAATTTGATAATTT+TGG - tig0015546:1713-1732 Msa1356230:CDS 20.0%
!!! TTGATAATTTTGGATATAGA+AGG - tig0015546:1723-1742 Msa1356230:CDS 20.0%
!! AACAATATGTAAGAGAAAAA+AGG + tig0015546:1861-1880 None:intergenic 20.0%
!! AATATATCATCAAAAGAAGT+TGG + tig0015546:2273-2292 None:intergenic 20.0%
!! TTACTGTAATTTCTATCAAT+AGG - tig0015546:2306-2325 Msa1356230:five_prime_UTR 20.0%
!! AATTAGAATAGTTTGAAAAG+TGG + tig0015546:3466-3485 None:intergenic 20.0%
!! TTTTATGTTATCCATTTGGT+AGG + tig0015546:1584-1603 None:intergenic 25.0%
!! TTTTGGATATAGAAGGAAAA+AGG - tig0015546:1730-1749 Msa1356230:CDS 25.0%
! TTTGGATATAGAAGGAAAAA+GGG - tig0015546:1731-1750 Msa1356230:CDS 25.0%
!! ATTGTTGGTTATTTGGAAAT+TGG - tig0015546:1935-1954 Msa1356230:intron 25.0%
!! ATGCATTTTGTTAGAATTTC+TGG - tig0015546:2035-2054 Msa1356230:CDS 25.0%
! CAACAACAATATTTGTAAAC+CGG + tig0015546:2080-2099 None:intergenic 25.0%
!! TAATTGGGTAGAATTTTTGT+GGG + tig0015546:2443-2462 None:intergenic 25.0%
!! ATAATTGGGTAGAATTTTTG+TGG + tig0015546:2444-2463 None:intergenic 25.0%
!!! CATCTGTTGCTTTAATAATT+GGG + tig0015546:2458-2477 None:intergenic 25.0%
! TCAATTAGATCAAGTTACAT+TGG + tig0015546:2578-2597 None:intergenic 25.0%
! ATTTCCACTATTGTTATCAT+TGG + tig0015546:2735-2754 None:intergenic 25.0%
! TCTAACATAAATTTCTCTGA+TGG - tig0015546:2788-2807 Msa1356230:five_prime_UTR 25.0%
!!! TTAGTATGCAATTTTGTTGT+TGG + tig0015546:2965-2984 None:intergenic 25.0%
! ACATAACAATAGATTACAAG+AGG + tig0015546:3088-3107 None:intergenic 25.0%
! TGAGTAAGACAAATTGAATT+CGG + tig0015546:3229-3248 None:intergenic 25.0%
!! TTTTATGTTATCCATTTGGT+AGG + tig0015546:1584-1603 None:intergenic 25.0%
!! TTTTGGATATAGAAGGAAAA+AGG - tig0015546:1730-1749 Msa1356230:CDS 25.0%
! TTTGGATATAGAAGGAAAAA+GGG - tig0015546:1731-1750 Msa1356230:CDS 25.0%
!! ATTGTTGGTTATTTGGAAAT+TGG - tig0015546:1935-1954 Msa1356230:intron 25.0%
!! ATGCATTTTGTTAGAATTTC+TGG - tig0015546:2035-2054 Msa1356230:CDS 25.0%
! CAACAACAATATTTGTAAAC+CGG + tig0015546:2080-2099 None:intergenic 25.0%
!! TAATTGGGTAGAATTTTTGT+GGG + tig0015546:2443-2462 None:intergenic 25.0%
!! ATAATTGGGTAGAATTTTTG+TGG + tig0015546:2444-2463 None:intergenic 25.0%
!!! CATCTGTTGCTTTAATAATT+GGG + tig0015546:2458-2477 None:intergenic 25.0%
! TCAATTAGATCAAGTTACAT+TGG + tig0015546:2578-2597 None:intergenic 25.0%
! ATTTCCACTATTGTTATCAT+TGG + tig0015546:2735-2754 None:intergenic 25.0%
! TCTAACATAAATTTCTCTGA+TGG - tig0015546:2788-2807 Msa1356230:five_prime_UTR 25.0%
!!! TTAGTATGCAATTTTGTTGT+TGG + tig0015546:2965-2984 None:intergenic 25.0%
! ACATAACAATAGATTACAAG+AGG + tig0015546:3088-3107 None:intergenic 25.0%
! TGAGTAAGACAAATTGAATT+CGG + tig0015546:3229-3248 None:intergenic 25.0%
AAATTGAGATACCTACCAAA+TGG - tig0015546:1570-1589 Msa1356230:three_prime_UTR 30.0%
TGATCAAGAATGAGATTTGT+GGG + tig0015546:1640-1659 None:intergenic 30.0%
TTGATCAAGAATGAGATTTG+TGG + tig0015546:1641-1660 None:intergenic 30.0%
TTGGATATAGAAGGAAAAAG+GGG - tig0015546:1732-1751 Msa1356230:CDS 30.0%
!!! TTCTTTTTTAACACCCCAAT+TGG - tig0015546:1823-1842 Msa1356230:CDS 30.0%
! ATTGTTATTCCCAATCACAT+TGG - tig0015546:1875-1894 Msa1356230:CDS 30.0%
TTCTATCAACCAATGTGATT+GGG + tig0015546:1887-1906 None:intergenic 30.0%
TTTCTATCAACCAATGTGAT+TGG + tig0015546:1888-1907 None:intergenic 30.0%
!! GGTTATTTGGAAATTGGTTT+TGG - tig0015546:1941-1960 Msa1356230:intron 30.0%
!!! TTGGAAATTGGTTTTGGAAT+TGG - tig0015546:1947-1966 Msa1356230:intron 30.0%
TCGAAAAGAGAAGAATGAAA+AGG - tig0015546:1991-2010 Msa1356230:intron 30.0%
TTCACTAGTTATGTTCTTCT+TGG - tig0015546:2204-2223 Msa1356230:intron 30.0%
TGACTCTCAATTATCTTGTT+TGG - tig0015546:2380-2399 Msa1356230:five_prime_UTR 30.0%
! ATCTTGTTTGGTTCAATCAA+CGG - tig0015546:2392-2411 Msa1356230:five_prime_UTR 30.0%
!! GCATCTGTTGCTTTAATAAT+TGG + tig0015546:2459-2478 None:intergenic 30.0%
TAAAGCAACAGATGCTAAAT+TGG - tig0015546:2464-2483 Msa1356230:intron 30.0%
GAGAAATTTATGTTAGAAGC+AGG + tig0015546:2785-2804 None:intergenic 30.0%
ACACTTAAAACTTGATCGTT+TGG - tig0015546:2901-2920 Msa1356230:five_prime_UTR 30.0%
! ATCGTTTGGACTTAACTTTT+AGG - tig0015546:2915-2934 Msa1356230:five_prime_UTR 30.0%
ATGAAAATATTGCCATGATG+GGG + tig0015546:2994-3013 None:intergenic 30.0%
GATGAAAATATTGCCATGAT+GGG + tig0015546:2995-3014 None:intergenic 30.0%
! ATGGCAATATTTTCATCCAA+AGG - tig0015546:2998-3017 Msa1356230:five_prime_UTR 30.0%
! TGGCAATATTTTCATCCAAA+GGG - tig0015546:2999-3018 Msa1356230:five_prime_UTR 30.0%
!! ATTAACACCTTTAGAATCTC+GGG + tig0015546:3040-3059 None:intergenic 30.0%
!! GATTAACACCTTTAGAATCT+CGG + tig0015546:3041-3060 None:intergenic 30.0%
ATTCAATTTGTCTTACTCAC+CGG - tig0015546:3230-3249 Msa1356230:five_prime_UTR 30.0%
! TCAAAGAGAAGTTGATCAAA+TGG - tig0015546:3429-3448 Msa1356230:five_prime_UTR 30.0%
AGAATAGTTTGAAAAGTGGA+AGG + tig0015546:3462-3481 None:intergenic 30.0%
AAATTGAGATACCTACCAAA+TGG - tig0015546:1570-1589 Msa1356230:three_prime_UTR 30.0%
TGATCAAGAATGAGATTTGT+GGG + tig0015546:1640-1659 None:intergenic 30.0%
TTGATCAAGAATGAGATTTG+TGG + tig0015546:1641-1660 None:intergenic 30.0%
TTGGATATAGAAGGAAAAAG+GGG - tig0015546:1732-1751 Msa1356230:CDS 30.0%
!!! TTCTTTTTTAACACCCCAAT+TGG - tig0015546:1823-1842 Msa1356230:CDS 30.0%
! ATTGTTATTCCCAATCACAT+TGG - tig0015546:1875-1894 Msa1356230:CDS 30.0%
TTCTATCAACCAATGTGATT+GGG + tig0015546:1887-1906 None:intergenic 30.0%
TTTCTATCAACCAATGTGAT+TGG + tig0015546:1888-1907 None:intergenic 30.0%
!! GGTTATTTGGAAATTGGTTT+TGG - tig0015546:1941-1960 Msa1356230:intron 30.0%
!!! TTGGAAATTGGTTTTGGAAT+TGG - tig0015546:1947-1966 Msa1356230:intron 30.0%
TCGAAAAGAGAAGAATGAAA+AGG - tig0015546:1991-2010 Msa1356230:intron 30.0%
TTCACTAGTTATGTTCTTCT+TGG - tig0015546:2204-2223 Msa1356230:intron 30.0%
TGACTCTCAATTATCTTGTT+TGG - tig0015546:2380-2399 Msa1356230:five_prime_UTR 30.0%
! ATCTTGTTTGGTTCAATCAA+CGG - tig0015546:2392-2411 Msa1356230:five_prime_UTR 30.0%
!! GCATCTGTTGCTTTAATAAT+TGG + tig0015546:2459-2478 None:intergenic 30.0%
TAAAGCAACAGATGCTAAAT+TGG - tig0015546:2464-2483 Msa1356230:intron 30.0%
GAGAAATTTATGTTAGAAGC+AGG + tig0015546:2785-2804 None:intergenic 30.0%
ACACTTAAAACTTGATCGTT+TGG - tig0015546:2901-2920 Msa1356230:five_prime_UTR 30.0%
! ATCGTTTGGACTTAACTTTT+AGG - tig0015546:2915-2934 Msa1356230:five_prime_UTR 30.0%
ATGAAAATATTGCCATGATG+GGG + tig0015546:2994-3013 None:intergenic 30.0%
GATGAAAATATTGCCATGAT+GGG + tig0015546:2995-3014 None:intergenic 30.0%
! ATGGCAATATTTTCATCCAA+AGG - tig0015546:2998-3017 Msa1356230:five_prime_UTR 30.0%
! TGGCAATATTTTCATCCAAA+GGG - tig0015546:2999-3018 Msa1356230:five_prime_UTR 30.0%
!! ATTAACACCTTTAGAATCTC+GGG + tig0015546:3040-3059 None:intergenic 30.0%
!! GATTAACACCTTTAGAATCT+CGG + tig0015546:3041-3060 None:intergenic 30.0%
ATTCAATTTGTCTTACTCAC+CGG - tig0015546:3230-3249 Msa1356230:five_prime_UTR 30.0%
! TCAAAGAGAAGTTGATCAAA+TGG - tig0015546:3429-3448 Msa1356230:five_prime_UTR 30.0%
AGAATAGTTTGAAAAGTGGA+AGG + tig0015546:3462-3481 None:intergenic 30.0%
!!! TTGGTTTTGGAATTGGTTGT+TGG - tig0015546:1954-1973 Msa1356230:intron 35.0%
AGTTATGTTCTTCTTGGCAA+CGG - tig0015546:2210-2229 Msa1356230:intron 35.0%
! TCTTGTTTGGTTCAATCAAC+GGG - tig0015546:2393-2412 Msa1356230:five_prime_UTR 35.0%
AAAATAGTAGTGTGCCTGAA+TGG - tig0015546:2653-2672 Msa1356230:five_prime_UTR 35.0%
CACTATTGTTATCATTGGCA+AGG + tig0015546:2730-2749 None:intergenic 35.0%
CTTGCCAATGATAACAATAG+TGG - tig0015546:2728-2747 Msa1356230:five_prime_UTR 35.0%
AGAAATCTAGCAGTCTAGTT+TGG - tig0015546:2847-2866 Msa1356230:five_prime_UTR 35.0%
TGAAAATATTGCCATGATGG+GGG + tig0015546:2993-3012 None:intergenic 35.0%
GGATGAAAATATTGCCATGA+TGG + tig0015546:2996-3015 None:intergenic 35.0%
TTGTCGATCTCTTCATTAGT+TGG + tig0015546:3203-3222 None:intergenic 35.0%
AAGGATACAAGGTTCTCAAA+AGG + tig0015546:3295-3314 None:intergenic 35.0%
ATAGCAGATTTAATAGCAGC+CGG + tig0015546:3326-3345 None:intergenic 35.0%
! GTCTGAAGTTGAGATTTCTT+GGG + tig0015546:3409-3428 None:intergenic 35.0%
! AGTCTGAAGTTGAGATTTCT+TGG + tig0015546:3410-3429 None:intergenic 35.0%
!!! TTGGTTTTGGAATTGGTTGT+TGG - tig0015546:1954-1973 Msa1356230:intron 35.0%
AGTTATGTTCTTCTTGGCAA+CGG - tig0015546:2210-2229 Msa1356230:intron 35.0%
! TCTTGTTTGGTTCAATCAAC+GGG - tig0015546:2393-2412 Msa1356230:five_prime_UTR 35.0%
AAAATAGTAGTGTGCCTGAA+TGG - tig0015546:2653-2672 Msa1356230:five_prime_UTR 35.0%
CACTATTGTTATCATTGGCA+AGG + tig0015546:2730-2749 None:intergenic 35.0%
CTTGCCAATGATAACAATAG+TGG - tig0015546:2728-2747 Msa1356230:five_prime_UTR 35.0%
AGAAATCTAGCAGTCTAGTT+TGG - tig0015546:2847-2866 Msa1356230:five_prime_UTR 35.0%
TGAAAATATTGCCATGATGG+GGG + tig0015546:2993-3012 None:intergenic 35.0%
GGATGAAAATATTGCCATGA+TGG + tig0015546:2996-3015 None:intergenic 35.0%
TTGTCGATCTCTTCATTAGT+TGG + tig0015546:3203-3222 None:intergenic 35.0%
AAGGATACAAGGTTCTCAAA+AGG + tig0015546:3295-3314 None:intergenic 35.0%
ATAGCAGATTTAATAGCAGC+CGG + tig0015546:3326-3345 None:intergenic 35.0%
! GTCTGAAGTTGAGATTTCTT+GGG + tig0015546:3409-3428 None:intergenic 35.0%
! AGTCTGAAGTTGAGATTTCT+TGG + tig0015546:3410-3429 None:intergenic 35.0%
TTTGTGGGATTGAGGAATTG+AGG + tig0015546:1625-1644 None:intergenic 40.0%
GAATGAGATTTGTGGGATTG+AGG + tig0015546:1633-1652 None:intergenic 40.0%
GAAGAACAGAAAGCCAATTG+GGG + tig0015546:1839-1858 None:intergenic 40.0%
GGAAGAACAGAAAGCCAATT+GGG + tig0015546:1840-1859 None:intergenic 40.0%
AGGAAGAACAGAAAGCCAAT+TGG + tig0015546:1841-1860 None:intergenic 40.0%
GAATTGGTTGTTGGAGATGA+AGG - tig0015546:1963-1982 Msa1356230:intron 40.0%
TTTGATGCAGTAGCCTCATT+TGG + tig0015546:2135-2154 None:intergenic 40.0%
! CTTGTTTGGTTCAATCAACG+GGG - tig0015546:2394-2413 Msa1356230:five_prime_UTR 40.0%
! TTGTTTGGTTCAATCAACGG+GGG - tig0015546:2395-2414 Msa1356230:five_prime_UTR 40.0%
TTAGAAGCAGGAAGAACATG+AGG + tig0015546:2773-2792 None:intergenic 40.0%
!! CTTAACTTTTAGGTCTCCAG+TGG - tig0015546:2925-2944 Msa1356230:five_prime_UTR 40.0%
GGATGTGTAATATAAGCCAC+TGG + tig0015546:2944-2963 None:intergenic 40.0%
TACAATGGTATTTGGCCCTT+TGG + tig0015546:3017-3036 None:intergenic 40.0%
ATCTCGGGTACAATGGTATT+TGG + tig0015546:3025-3044 None:intergenic 40.0%
!! CTTTAGAATCTCGGGTACAA+TGG + tig0015546:3032-3051 None:intergenic 40.0%
CATTGTACCCGAGATTCTAA+AGG - tig0015546:3030-3049 Msa1356230:five_prime_UTR 40.0%
TGAGAACCTTGTATCCTTCT+TGG - tig0015546:3297-3316 Msa1356230:five_prime_UTR 40.0%
AAATCTGCTATCATGACCAC+AGG - tig0015546:3335-3354 Msa1356230:five_prime_UTR 40.0%
CAAGAGTTTCTTCTTACCTG+TGG + tig0015546:3354-3373 None:intergenic 40.0%
TTTGTGGGATTGAGGAATTG+AGG + tig0015546:1625-1644 None:intergenic 40.0%
GAATGAGATTTGTGGGATTG+AGG + tig0015546:1633-1652 None:intergenic 40.0%
GAAGAACAGAAAGCCAATTG+GGG + tig0015546:1839-1858 None:intergenic 40.0%
GGAAGAACAGAAAGCCAATT+GGG + tig0015546:1840-1859 None:intergenic 40.0%
AGGAAGAACAGAAAGCCAAT+TGG + tig0015546:1841-1860 None:intergenic 40.0%
GAATTGGTTGTTGGAGATGA+AGG - tig0015546:1963-1982 Msa1356230:intron 40.0%
TTTGATGCAGTAGCCTCATT+TGG + tig0015546:2135-2154 None:intergenic 40.0%
! CTTGTTTGGTTCAATCAACG+GGG - tig0015546:2394-2413 Msa1356230:five_prime_UTR 40.0%
! TTGTTTGGTTCAATCAACGG+GGG - tig0015546:2395-2414 Msa1356230:five_prime_UTR 40.0%
TTAGAAGCAGGAAGAACATG+AGG + tig0015546:2773-2792 None:intergenic 40.0%
!! CTTAACTTTTAGGTCTCCAG+TGG - tig0015546:2925-2944 Msa1356230:five_prime_UTR 40.0%
GGATGTGTAATATAAGCCAC+TGG + tig0015546:2944-2963 None:intergenic 40.0%
TACAATGGTATTTGGCCCTT+TGG + tig0015546:3017-3036 None:intergenic 40.0%
ATCTCGGGTACAATGGTATT+TGG + tig0015546:3025-3044 None:intergenic 40.0%
!! CTTTAGAATCTCGGGTACAA+TGG + tig0015546:3032-3051 None:intergenic 40.0%
CATTGTACCCGAGATTCTAA+AGG - tig0015546:3030-3049 Msa1356230:five_prime_UTR 40.0%
TGAGAACCTTGTATCCTTCT+TGG - tig0015546:3297-3316 Msa1356230:five_prime_UTR 40.0%
AAATCTGCTATCATGACCAC+AGG - tig0015546:3335-3354 Msa1356230:five_prime_UTR 40.0%
CAAGAGTTTCTTCTTACCTG+TGG + tig0015546:3354-3373 None:intergenic 40.0%
ACCCAGAAAAGCTCAGTTCA+TGG - tig0015546:1694-1713 Msa1356230:CDS 45.0%
! GCCATGAACTGAGCTTTTCT+GGG + tig0015546:1698-1717 None:intergenic 45.0%
! TGCCATGAACTGAGCTTTTC+TGG + tig0015546:1699-1718 None:intergenic 45.0%
!! TGGCAATGATGGACCAAATG+AGG - tig0015546:2119-2138 Msa1356230:five_prime_UTR 45.0%
! CTGCATCAAATCTAGCTCCT+TGG - tig0015546:2145-2164 Msa1356230:intron 45.0%
GCTCCTTGGTATATCACAGT+TGG - tig0015546:2159-2178 Msa1356230:intron 45.0%
GCACCAACTGTGATATACCA+AGG + tig0015546:2165-2184 None:intergenic 45.0%
GAACTCTCTATCCATTGTGC+TGG + tig0015546:2187-2206 None:intergenic 45.0%
TGGCAACGGTTTAAGCTTCA+AGG - tig0015546:2224-2243 Msa1356230:intron 45.0%
TCTGGATCACAAGAAGGTGA+AGG - tig0015546:2628-2647 Msa1356230:five_prime_UTR 45.0%
CTGGATCACAAGAAGGTGAA+GGG - tig0015546:2629-2648 Msa1356230:five_prime_UTR 45.0%
ATAGTAGTGTGCCTGAATGG+AGG - tig0015546:2656-2675 Msa1356230:five_prime_UTR 45.0%
CTGAATGGAGGAGTGGATAA+GGG - tig0015546:2668-2687 Msa1356230:five_prime_UTR 45.0%
! TCTAGCAGTCTAGTTTGGTG+TGG - tig0015546:2852-2871 Msa1356230:five_prime_UTR 45.0%
CATGTCAAGCCCTCACATTT+CGG - tig0015546:3259-3278 Msa1356230:five_prime_UTR 45.0%
ATGTCAAGCCCTCACATTTC+GGG - tig0015546:3260-3279 Msa1356230:five_prime_UTR 45.0%
CTGCAATTCCCGAAATGTGA+GGG + tig0015546:3271-3290 None:intergenic 45.0%
CTTGTATCCTTCTTGGAGTC+CGG - tig0015546:3304-3323 Msa1356230:five_prime_UTR 45.0%
ACCCAGAAAAGCTCAGTTCA+TGG - tig0015546:1694-1713 Msa1356230:CDS 45.0%
! GCCATGAACTGAGCTTTTCT+GGG + tig0015546:1698-1717 None:intergenic 45.0%
! TGCCATGAACTGAGCTTTTC+TGG + tig0015546:1699-1718 None:intergenic 45.0%
!! TGGCAATGATGGACCAAATG+AGG - tig0015546:2119-2138 Msa1356230:five_prime_UTR 45.0%
! CTGCATCAAATCTAGCTCCT+TGG - tig0015546:2145-2164 Msa1356230:intron 45.0%
GCTCCTTGGTATATCACAGT+TGG - tig0015546:2159-2178 Msa1356230:intron 45.0%
GCACCAACTGTGATATACCA+AGG + tig0015546:2165-2184 None:intergenic 45.0%
GAACTCTCTATCCATTGTGC+TGG + tig0015546:2187-2206 None:intergenic 45.0%
TGGCAACGGTTTAAGCTTCA+AGG - tig0015546:2224-2243 Msa1356230:intron 45.0%
TCTGGATCACAAGAAGGTGA+AGG - tig0015546:2628-2647 Msa1356230:five_prime_UTR 45.0%
CTGGATCACAAGAAGGTGAA+GGG - tig0015546:2629-2648 Msa1356230:five_prime_UTR 45.0%
ATAGTAGTGTGCCTGAATGG+AGG - tig0015546:2656-2675 Msa1356230:five_prime_UTR 45.0%
CTGAATGGAGGAGTGGATAA+GGG - tig0015546:2668-2687 Msa1356230:five_prime_UTR 45.0%
! TCTAGCAGTCTAGTTTGGTG+TGG - tig0015546:2852-2871 Msa1356230:five_prime_UTR 45.0%
CATGTCAAGCCCTCACATTT+CGG - tig0015546:3259-3278 Msa1356230:five_prime_UTR 45.0%
ATGTCAAGCCCTCACATTTC+GGG - tig0015546:3260-3279 Msa1356230:five_prime_UTR 45.0%
CTGCAATTCCCGAAATGTGA+GGG + tig0015546:3271-3290 None:intergenic 45.0%
CTTGTATCCTTCTTGGAGTC+CGG - tig0015546:3304-3323 Msa1356230:five_prime_UTR 45.0%
! GTTGTTGTTGCTTGCTCTGC+TGG - tig0015546:2099-2118 Msa1356230:five_prime_UTR 50.0%
TGTGATCCAGAGTCCCATTC+TGG + tig0015546:2619-2638 None:intergenic 50.0%
TGGGACTCTGGATCACAAGA+AGG - tig0015546:2622-2641 Msa1356230:five_prime_UTR 50.0%
CCTTATCCACTCCTCCATTC+AGG + tig0015546:2670-2689 None:intergenic 50.0%
CCTGAATGGAGGAGTGGATA+AGG - tig0015546:2667-2686 Msa1356230:five_prime_UTR 50.0%
AGGAGTGGATAAGGGAGAAC+AGG - tig0015546:2676-2695 Msa1356230:five_prime_UTR 50.0%
GGAGAACAGGCTCTTATAGC+TGG - tig0015546:2689-2708 Msa1356230:five_prime_UTR 50.0%
!! GCTCTTATAGCTGGTGCTGT+AGG - tig0015546:2698-2717 Msa1356230:five_prime_UTR 50.0%
!! TATAGCTGGTGCTGTAGGAG+TGG - tig0015546:2703-2722 Msa1356230:five_prime_UTR 50.0%
TACTAAACCAGCCCCCATCA+TGG - tig0015546:2979-2998 Msa1356230:five_prime_UTR 50.0%
! AATATTGCCATGATGGGGGC+TGG + tig0015546:2989-3008 None:intergenic 50.0%
!! ACACCTGGTGCTGTGATATC+AGG + tig0015546:3146-3165 None:intergenic 50.0%
TATCACAGCACCAGGTGTGT+CGG - tig0015546:3148-3167 Msa1356230:five_prime_UTR 50.0%
TGGCTCTTCAGCTTCAGTGA+AGG + tig0015546:3183-3202 None:intergenic 50.0%
CGAAATGTGAGGGCTTGACA+TGG + tig0015546:3261-3280 None:intergenic 50.0%
CCTGCAATTCCCGAAATGTG+AGG + tig0015546:3272-3291 None:intergenic 50.0%
CCTCACATTTCGGGAATTGC+AGG - tig0015546:3269-3288 Msa1356230:five_prime_UTR 50.0%
CGGACTCCAAGAAGGATACA+AGG + tig0015546:3306-3325 None:intergenic 50.0%
! GTTGTTGTTGCTTGCTCTGC+TGG - tig0015546:2099-2118 Msa1356230:five_prime_UTR 50.0%
TGTGATCCAGAGTCCCATTC+TGG + tig0015546:2619-2638 None:intergenic 50.0%
TGGGACTCTGGATCACAAGA+AGG - tig0015546:2622-2641 Msa1356230:five_prime_UTR 50.0%
CCTTATCCACTCCTCCATTC+AGG + tig0015546:2670-2689 None:intergenic 50.0%
CCTGAATGGAGGAGTGGATA+AGG - tig0015546:2667-2686 Msa1356230:five_prime_UTR 50.0%
AGGAGTGGATAAGGGAGAAC+AGG - tig0015546:2676-2695 Msa1356230:five_prime_UTR 50.0%
GGAGAACAGGCTCTTATAGC+TGG - tig0015546:2689-2708 Msa1356230:five_prime_UTR 50.0%
!! GCTCTTATAGCTGGTGCTGT+AGG - tig0015546:2698-2717 Msa1356230:five_prime_UTR 50.0%
!! TATAGCTGGTGCTGTAGGAG+TGG - tig0015546:2703-2722 Msa1356230:five_prime_UTR 50.0%
TACTAAACCAGCCCCCATCA+TGG - tig0015546:2979-2998 Msa1356230:five_prime_UTR 50.0%
! AATATTGCCATGATGGGGGC+TGG + tig0015546:2989-3008 None:intergenic 50.0%
!! ACACCTGGTGCTGTGATATC+AGG + tig0015546:3146-3165 None:intergenic 50.0%
TATCACAGCACCAGGTGTGT+CGG - tig0015546:3148-3167 Msa1356230:five_prime_UTR 50.0%
TGGCTCTTCAGCTTCAGTGA+AGG + tig0015546:3183-3202 None:intergenic 50.0%
CGAAATGTGAGGGCTTGACA+TGG + tig0015546:3261-3280 None:intergenic 50.0%
CCTGCAATTCCCGAAATGTG+AGG + tig0015546:3272-3291 None:intergenic 50.0%
CCTCACATTTCGGGAATTGC+AGG - tig0015546:3269-3288 Msa1356230:five_prime_UTR 50.0%
CGGACTCCAAGAAGGATACA+AGG + tig0015546:3306-3325 None:intergenic 50.0%
GCTTGCTCTGCTGGCAATGA+TGG - tig0015546:2108-2127 Msa1356230:five_prime_UTR 55.0%
!! CACAGTTGGTGCCAGCACAA+TGG - tig0015546:2173-2192 Msa1356230:intron 55.0%
TGCAGCGAGCTTTGCCAGAA+TGG - tig0015546:2602-2621 Msa1356230:five_prime_UTR 55.0%
GCAGCGAGCTTTGCCAGAAT+GGG - tig0015546:2603-2622 Msa1356230:five_prime_UTR 55.0%
GCTTTGCCAGAATGGGACTC+TGG - tig0015546:2610-2629 Msa1356230:five_prime_UTR 55.0%
AGTGTGCCTGAATGGAGGAG+TGG - tig0015546:2661-2680 Msa1356230:five_prime_UTR 55.0%
CAGCCTGATATCACAGCACC+AGG - tig0015546:3140-3159 Msa1356230:five_prime_UTR 55.0%
GCTGCTATAACCGACACACC+TGG + tig0015546:3161-3180 None:intergenic 55.0%
AGGGCTTGACATGGATGTGC+CGG + tig0015546:3252-3271 None:intergenic 55.0%
ATAGCAGCCGGACTCCAAGA+AGG + tig0015546:3314-3333 None:intergenic 55.0%
GCTTGCTCTGCTGGCAATGA+TGG - tig0015546:2108-2127 Msa1356230:five_prime_UTR 55.0%
!! CACAGTTGGTGCCAGCACAA+TGG - tig0015546:2173-2192 Msa1356230:intron 55.0%
TGCAGCGAGCTTTGCCAGAA+TGG - tig0015546:2602-2621 Msa1356230:five_prime_UTR 55.0%
GCAGCGAGCTTTGCCAGAAT+GGG - tig0015546:2603-2622 Msa1356230:five_prime_UTR 55.0%
GCTTTGCCAGAATGGGACTC+TGG - tig0015546:2610-2629 Msa1356230:five_prime_UTR 55.0%
AGTGTGCCTGAATGGAGGAG+TGG - tig0015546:2661-2680 Msa1356230:five_prime_UTR 55.0%
CAGCCTGATATCACAGCACC+AGG - tig0015546:3140-3159 Msa1356230:five_prime_UTR 55.0%
GCTGCTATAACCGACACACC+TGG + tig0015546:3161-3180 None:intergenic 55.0%
AGGGCTTGACATGGATGTGC+CGG + tig0015546:3252-3271 None:intergenic 55.0%
ATAGCAGCCGGACTCCAAGA+AGG + tig0015546:3314-3333 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0015546 gene 1546 3531 1546 ID=Msa1356230;Name=Msa1356230
tig0015546 mRNA 1546 3531 1546 ID=Msa1356230-mRNA-1;Parent=Msa1356230;Name=Msa1356230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=1261|1|1|1|1|1|4|128|104
tig0015546 exon 1546 1934 1546 ID=Msa1356230-mRNA-1:exon:9814;Parent=Msa1356230-mRNA-1
tig0015546 exon 2026 2140 2026 ID=Msa1356230-mRNA-1:exon:9813;Parent=Msa1356230-mRNA-1
tig0015546 exon 2234 2470 2234 ID=Msa1356230-mRNA-1:exon:9812;Parent=Msa1356230-mRNA-1
tig0015546 exon 2569 3531 2569 ID=Msa1356230-mRNA-1:exon:9811;Parent=Msa1356230-mRNA-1
tig0015546 five_prime_UTR 2569 3531 2569 ID=Msa1356230-mRNA-1:five_prime_utr;Parent=Msa1356230-mRNA-1
tig0015546 five_prime_UTR 2234 2470 2234 ID=Msa1356230-mRNA-1:five_prime_utr;Parent=Msa1356230-mRNA-1
tig0015546 five_prime_UTR 2080 2140 2080 ID=Msa1356230-mRNA-1:five_prime_utr;Parent=Msa1356230-mRNA-1
tig0015546 CDS 2026 2079 2026 ID=Msa1356230-mRNA-1:cds;Parent=Msa1356230-mRNA-1
tig0015546 CDS 1674 1934 1674 ID=Msa1356230-mRNA-1:cds;Parent=Msa1356230-mRNA-1
tig0015546 three_prime_UTR 1546 1673 1546 ID=Msa1356230-mRNA-1:three_prime_utr;Parent=Msa1356230-mRNA-1
Gene Sequence

>Msa1356230

ATGGCAATATTTTCATCCAAAGGGCCAAATACCATTGTACCCGAGATTCTAAAGCCTGATATCACAGCACCAGGTGTGTCGGTTATAGCAGCCTTCACTGAAGCTGAAGAGCCAACTAATGAAGAGATCGACAATCGCCGAATTCAATTTGTCTTACTCACCGGCACATCCATGTCAAGCCCTCACATTTCGGGAATTGCAGGCCTTTTGAGAACCTTGTATCCTTCTTGGAGTCCGGCTGCTATTAAATCTGCTATCATGACCACAGGTAAGAAGAAACTCTTGAAGTTTCTCATACTTCATGTCATTTCTTGA
Protein sequence

>Msa1356230

MAIFSSKGPNTIVPEILKPDITAPGVSVIAAFTEAEEPTNEEIDNRRIQFVLLTGTSMSSPHISGIAGLLRTLYPSWSPAAIKSAIMTTGKKKLLKFLILHVIS*