AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0550280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550280 MtrunA17_Chr4g0035101 90.083 363 16 2 1 362 1 344 0.0 664
Msa0550280 MtrunA17_Chr3g0144641 53.444 421 114 18 1 362 1 398 1.28e-122 359
Msa0550280 MtrunA17_Chr7g0234761 51.659 211 78 7 72 264 220 424 7.95e-53 182
Msa0550280 MtrunA17_Chr5g0421111 46.332 259 102 13 122 362 319 558 2.24e-49 174
Msa0550280 MtrunA17_Chr8g0339091 53.005 183 71 6 79 251 205 382 2.72e-46 164
Msa0550280 MtrunA17_Chr8g0365941 54.088 159 68 3 91 248 238 392 4.22e-46 163
Msa0550280 MtrunA17_Chr1g0152131 45.972 211 72 6 141 337 226 408 5.81e-45 159
Msa0550280 MtrunA17_Chr8g0363921 75.258 97 24 0 158 254 148 244 3.54e-43 151
Msa0550280 MtrunA17_Chr5g0415291 59.701 134 42 2 117 250 79 200 1.13e-42 148
Msa0550280 MtrunA17_Chr7g0258161 74.194 93 24 0 154 246 166 258 5.20e-42 149
Msa0550280 MtrunA17_Chr1g0174161 62.712 118 35 3 140 253 143 255 1.84e-41 147
Msa0550280 MtrunA17_Chr8g0385911 82.143 84 14 1 158 240 142 225 1.14e-39 143
Msa0550280 MtrunA17_Chr3g0131771 75.000 92 20 1 158 246 144 235 1.55e-39 142
Msa0550280 MtrunA17_Chr6g0484271 48.701 154 61 3 85 238 97 232 1.36e-33 125
Msa0550280 MtrunA17_Chr4g0074161 71.212 66 18 1 161 226 206 270 1.28e-21 95.1
Msa0550280 MtrunA17_Chr1g0190731 68.182 66 20 1 161 226 193 257 1.37e-20 91.7
Msa0550280 MtrunA17_Chr4g0032951 56.923 65 28 0 161 225 355 419 3.85e-15 76.3
Msa0550280 MtrunA17_Chr1g0182791 52.174 69 32 1 163 231 319 386 6.93e-15 75.9
Msa0550280 MtrunA17_Chr4g0014771 53.846 65 30 0 161 225 118 182 1.28e-14 71.6
Msa0550280 MtrunA17_Chr2g0329041 52.308 65 31 0 159 223 330 394 2.08e-14 73.9
Msa0550280 MtrunA17_Chr1g0193321 45.205 73 39 1 163 235 464 535 2.21e-14 74.7
Msa0550280 MtrunA17_Chr1g0174271 48.718 78 38 1 154 229 289 366 2.39e-14 73.6
Msa0550280 MtrunA17_Chr1g0152651 45.455 88 43 2 138 225 255 337 6.91e-14 72.0
Msa0550280 MtrunA17_Chr7g0258181 45.783 83 43 1 154 234 234 316 7.22e-14 71.6
Msa0550280 MtrunA17_Chr1g0184841 63.636 66 23 1 161 226 120 184 7.99e-14 71.2
Msa0550280 MtrunA17_Chr1g0153241 40.909 88 50 2 141 227 64 150 1.20e-12 67.4
Msa0550280 MtrunA17_Chr3g0087771 49.231 65 33 0 161 225 322 386 2.39e-12 67.8
Msa0550280 MtrunA17_Chr7g0271251 34.706 170 99 7 127 289 396 560 3.26e-12 67.8
Msa0550280 MtrunA17_Chr8g0351881 47.692 65 34 0 161 225 316 380 4.58e-12 66.6
Msa0550280 MtrunA17_Chr7g0262691 37.097 124 57 5 111 226 206 316 1.16e-11 65.9
Msa0550280 MtrunA17_Chr3g0095391 36.893 103 60 3 163 262 311 411 1.94e-11 65.1
Msa0550280 MtrunA17_Chr5g0393721 34.146 123 63 3 103 225 183 287 3.08e-11 63.9
Msa0550280 MtrunA17_Chr3g0134741 51.562 64 30 1 162 225 230 292 3.69e-11 63.5
Msa0550280 MtrunA17_Chr7g0270871 43.939 66 36 1 172 237 213 277 4.62e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550280 AT4G34530.1 38.798 366 139 11 12 332 5 330 1.98e-58 192
Msa0550280 AT2G18300.1 59.627 161 55 5 92 251 124 275 4.04e-52 176
Msa0550280 AT1G68920.3 42.105 285 117 7 90 361 235 484 4.01e-51 177
Msa0550280 AT2G18300.2 60.897 156 51 5 92 246 124 270 5.00e-51 173
Msa0550280 AT1G68920.1 40.956 293 125 6 82 361 228 485 5.36e-51 177
Msa0550280 AT1G68920.4 40.956 293 125 6 82 361 228 485 5.36e-51 177
Msa0550280 AT1G68920.2 40.956 293 125 6 82 361 228 485 5.36e-51 177
Msa0550280 AT2G18300.3 60.897 156 51 5 92 246 124 270 1.23e-50 173
Msa0550280 AT3G07340.1 53.093 194 70 4 81 274 195 367 1.67e-50 175
Msa0550280 AT3G07340.2 53.093 194 70 4 81 274 195 367 1.67e-50 175
Msa0550280 AT3G23690.1 48.636 220 93 5 77 284 98 309 4.07e-49 169
Msa0550280 AT5G48560.2 50.000 198 93 3 57 248 192 389 5.50e-49 171
Msa0550280 AT5G48560.1 50.000 198 93 3 57 248 192 389 1.29e-48 171
Msa0550280 AT1G26260.3 57.325 157 56 1 104 260 126 271 1.30e-48 167
Msa0550280 AT1G26260.5 57.325 157 56 1 104 260 155 300 4.07e-48 167
Msa0550280 AT1G26260.4 57.325 157 56 1 104 260 177 322 6.93e-48 167
Msa0550280 AT1G26260.1 57.325 157 56 1 104 260 177 322 6.93e-48 167
Msa0550280 AT1G26260.6 57.325 157 56 1 104 260 177 322 6.93e-48 167
Msa0550280 AT1G26260.2 57.325 157 56 1 104 260 177 322 6.93e-48 167
Msa0550280 AT4G36540.2 53.254 169 58 4 92 260 93 240 1.82e-47 163
Msa0550280 AT4G36540.1 56.129 155 47 4 92 246 93 226 1.36e-46 161
Msa0550280 AT1G10120.2 46.544 217 78 5 126 342 174 352 4.34e-46 161
Msa0550280 AT1G10120.1 46.544 217 78 5 126 342 174 352 4.34e-46 161
Msa0550280 AT1G59640.1 63.636 132 41 2 127 252 97 227 7.37e-45 155
Msa0550280 AT2G42300.4 71.134 97 28 0 151 247 175 271 8.91e-44 152
Msa0550280 AT3G57800.2 63.415 123 40 2 152 274 195 312 2.66e-43 154
Msa0550280 AT1G59640.2 65.354 127 37 2 127 247 97 222 3.08e-43 153
Msa0550280 AT5G50915.3 54.777 157 53 6 112 262 85 229 5.99e-43 150
Msa0550280 AT5G50915.4 54.777 157 53 6 112 262 85 229 5.99e-43 150
Msa0550280 AT5G50915.1 54.777 157 53 6 112 262 85 229 2.18e-42 149
Msa0550280 AT5G50915.2 54.777 157 53 6 112 262 85 229 2.18e-42 149
Msa0550280 AT2G42300.1 70.833 96 28 0 151 246 175 270 2.84e-42 150
Msa0550280 AT5G62610.2 77.647 85 15 1 152 232 140 224 1.20e-37 137
Msa0550280 AT5G62610.1 77.647 85 15 1 152 232 140 224 1.20e-37 137
Msa0550280 AT1G18400.2 76.250 80 19 0 159 238 143 222 2.29e-35 130
Msa0550280 AT1G18400.1 76.250 80 19 0 159 238 143 222 4.56e-35 129
Msa0550280 AT1G73830.2 77.215 79 18 0 160 238 146 224 6.57e-35 129
Msa0550280 AT1G73830.1 77.215 79 18 0 160 238 146 224 7.32e-35 129
Msa0550280 AT3G57800.1 45.882 170 40 3 152 274 195 359 1.93e-34 131
Msa0550280 AT1G25330.1 64.286 98 34 1 152 248 94 191 2.69e-34 126
Msa0550280 AT5G58010.1 58.696 92 30 3 149 236 90 177 5.36e-23 97.8
Msa0550280 AT2G24260.2 56.471 85 36 1 142 226 16 99 2.79e-22 94.7
Msa0550280 AT4G30980.1 71.212 66 18 1 161 226 130 194 7.63e-22 94.7
Msa0550280 AT4G30980.2 71.212 66 18 1 161 226 178 242 1.16e-21 95.1
Msa0550280 AT2G24260.1 56.471 85 36 1 142 226 119 202 2.02e-21 94.4
Msa0550280 AT2G42300.5 70.000 50 15 0 151 200 175 224 3.50e-18 82.8
Msa0550280 AT2G42300.3 70.000 50 15 0 151 200 175 224 3.50e-18 82.8
Msa0550280 AT2G42300.2 70.000 50 15 0 151 200 175 224 4.22e-18 82.8
Msa0550280 AT2G20180.7 53.968 63 28 1 164 226 210 271 2.03e-14 74.3
Msa0550280 AT2G20180.1 53.968 63 28 1 164 226 210 271 2.03e-14 74.3
Msa0550280 AT2G20180.5 53.968 63 28 1 164 226 210 271 2.03e-14 74.3
Msa0550280 AT2G20180.4 53.968 63 28 1 164 226 281 342 2.26e-14 74.7
Msa0550280 AT2G20180.6 53.968 63 28 1 164 226 281 342 2.26e-14 74.7
Msa0550280 AT2G20180.2 53.968 63 28 1 164 226 281 342 2.26e-14 74.7
Msa0550280 AT2G20180.3 53.968 63 28 1 164 226 281 342 2.26e-14 74.7
Msa0550280 AT1G09530.3 46.479 71 37 1 156 226 332 401 3.51e-14 73.9
Msa0550280 AT1G09530.2 46.479 71 37 1 156 226 332 401 3.51e-14 73.9
Msa0550280 AT1G09530.5 46.479 71 37 1 156 226 332 401 3.51e-14 73.9
Msa0550280 AT1G09530.1 46.479 71 37 1 156 226 332 401 3.51e-14 73.9
Msa0550280 AT1G09530.4 46.479 71 37 1 156 226 332 401 3.51e-14 73.9
Msa0550280 AT1G09530.6 46.479 71 37 1 156 226 332 401 3.51e-14 73.9
Msa0550280 AT4G09180.1 50.746 67 33 0 159 225 182 248 6.57e-14 71.6
Msa0550280 AT2G42280.1 36.667 120 72 2 105 224 227 342 7.21e-14 72.4
Msa0550280 AT2G42280.3 37.705 122 70 3 105 224 227 344 7.88e-14 72.4
Msa0550280 AT4G28790.1 35.043 117 67 3 163 278 273 381 8.93e-14 72.4
Msa0550280 AT1G51140.1 54.688 64 29 0 161 224 304 367 9.65e-14 72.4
Msa0550280 AT4G28800.1 29.258 229 129 8 50 270 147 350 1.51e-13 72.0
Msa0550280 AT4G02590.3 51.724 87 33 2 148 226 62 147 1.57e-13 70.1
Msa0550280 AT4G02590.2 51.724 87 33 2 148 226 125 210 2.36e-13 70.5
Msa0550280 AT4G02590.1 51.724 87 33 2 148 226 125 210 2.36e-13 70.5
Msa0550280 AT1G03040.2 62.121 66 24 1 161 226 139 203 2.94e-13 70.1
Msa0550280 AT1G35460.1 50.769 65 32 0 161 225 181 245 3.07e-13 69.3
Msa0550280 AT1G03040.1 62.121 66 24 1 161 226 144 208 3.22e-13 70.1
Msa0550280 AT4G28815.2 55.172 58 25 1 164 221 71 127 3.70e-13 68.9
Msa0550280 AT2G20180.8 52.459 61 28 1 163 223 280 339 4.25e-13 70.1
Msa0550280 AT1G03040.3 62.121 66 24 1 161 226 144 208 5.69e-13 69.7
Msa0550280 AT4G28815.1 48.571 70 34 2 164 232 147 215 1.06e-12 68.6
Msa0550280 AT1G27740.1 38.938 113 68 1 113 225 119 230 2.11e-12 67.0
Msa0550280 AT4G28800.2 32.778 180 97 7 50 222 159 321 6.32e-12 67.0
Msa0550280 AT2G14760.1 39.655 116 56 4 120 225 189 300 1.12e-11 65.5
Msa0550280 AT2G46970.3 41.250 80 41 2 159 233 194 272 1.14e-11 65.9
Msa0550280 AT2G46970.2 41.250 80 41 2 159 233 185 263 1.15e-11 65.9
Msa0550280 AT2G46970.1 41.250 80 41 2 159 233 216 294 1.18e-11 66.2
Msa0550280 AT4G33880.1 43.011 93 49 3 136 225 238 329 3.99e-11 64.3
Msa0550280 AT4G00050.2 49.231 65 32 1 161 225 105 168 6.92e-11 63.2

Find 83 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGTTTGCTCAGTGATTT+TGG 0.069577 4_1:-45688598 None:intergenic
TTAAAGTACATAACATATTT+TGG 0.158845 4_1:+45690597 Msa0550280:three_prime_UTR
CATCTGATCCATTGCAAATT+AGG 0.160930 4_1:+45690749 Msa0550280:three_prime_UTR
CACTTTGATCCTCTTGTCTT+TGG 0.165749 4_1:-45688556 None:intergenic
GGGATAGTCCCTCCGGAAAT+TGG 0.186320 4_1:+45689902 Msa0550280:CDS
GTTGATCTTGTTGTTTGTTT+TGG 0.200209 4_1:-45688226 None:intergenic
TTACATTGTCTTAACACTTC+TGG 0.231856 4_1:+45688252 Msa0550280:CDS
CTGTTACATTCTCCTTCTTT+TGG 0.239090 4_1:+45690658 Msa0550280:three_prime_UTR
GACATTTCTGATTGCATCAT+TGG 0.242720 4_1:-45689806 None:intergenic
TTCCTTCGAACCCCAATTTC+CGG 0.243538 4_1:-45689914 None:intergenic
AATTTGTATCTACCTGTAAA+TGG 0.288243 4_1:-45690193 None:intergenic
TTTCCTTCTCAACCATTTAC+AGG 0.299320 4_1:+45690181 Msa0550280:CDS
GATGAGTCAAGAAAGATTTC+AGG 0.310824 4_1:-45689965 None:intergenic
TGCAGGTTTAATGTCCGTTT+TGG 0.317732 4_1:-45690818 None:intergenic
GTTGGTTGTGATTCTGCTCT+TGG 0.325858 4_1:+45688435 Msa0550280:CDS
CGCTATGCTCTTTGTAGAAA+TGG 0.333391 4_1:-45688121 None:intergenic
GCATTGTGTCCATACCTCTT+TGG 0.342272 4_1:-45689530 None:intergenic
TGGTGGATTGATAAACAATA+TGG 0.350380 4_1:+45689880 Msa0550280:CDS
GGATAGTCCCTCCGGAAATT+GGG 0.350458 4_1:+45689903 Msa0550280:CDS
AACAAAATTGCAGGCAAAGC+TGG 0.365496 4_1:+45689249 Msa0550280:CDS
CATTAAACCTGCAAGAAATT+CGG 0.370651 4_1:+45690828 Msa0550280:three_prime_UTR
CAACACTGTCATGTCGGAAA+TGG 0.383436 4_1:-45688292 None:intergenic
TCATGAACCGAATTTCTTGC+AGG 0.397044 4_1:-45690835 None:intergenic
ATCTGATCCATTGCAAATTA+GGG 0.401608 4_1:+45690750 Msa0550280:three_prime_UTR
CAATGTTGAAGTCAAGTCTT+GGG 0.403046 4_1:-45689495 None:intergenic
CAAAAGAAGGAGAATGTAAC+AGG 0.403302 4_1:-45690657 None:intergenic
CCAGGATGTAACAAAATTGC+AGG 0.430070 4_1:+45689240 Msa0550280:CDS
TGATGAATTGTTTGCCAAAG+AGG 0.438799 4_1:+45689516 Msa0550280:CDS
TTCAGAGTGTGCAATGTACT+AGG 0.439399 4_1:-45688190 None:intergenic
GCTCGAACATGAATGTAATC+AGG 0.466193 4_1:-45688714 None:intergenic
TCAATGTTGAAGTCAAGTCT+TGG 0.479932 4_1:-45689496 None:intergenic
GAATTGAATTGAAGATATGC+AGG 0.482069 4_1:-45689833 None:intergenic
AAAGAGAGAGATTTGTGAGC+AGG 0.484444 4_1:+45690726 Msa0550280:three_prime_UTR
GGTTCCAAGGAAAATTCAAA+AGG 0.493943 4_1:+45688669 Msa0550280:CDS
TCGAAGAAACCGTTAAGAAA+AGG 0.498481 4_1:+45688505 Msa0550280:CDS
ATCAAAGTGAGTGTTGAAGA+AGG 0.498660 4_1:+45688570 Msa0550280:CDS
AACACTTCTGGAAATCTAGT+AGG 0.504501 4_1:+45688264 Msa0550280:CDS
TAACTTGAATGTCCAAAAGA+AGG 0.504694 4_1:-45690670 None:intergenic
TACGTGCTAAACTACGCACT+TGG 0.506535 4_1:-45688161 None:intergenic
ATATCCTACCATCCTCAACT+TGG 0.507057 4_1:+45690101 Msa0550280:CDS
AAAATCACCTTCCCAAGTTG+AGG 0.508180 4_1:-45690113 None:intergenic
CAATATGGGGATAGTCCCTC+CGG 0.510922 4_1:+45689895 Msa0550280:CDS
TATTTGCAAGATTTAGTACC+AGG 0.510967 4_1:+45689222 Msa0550280:CDS
GGTGGATTGATAAACAATAT+GGG 0.517497 4_1:+45689881 Msa0550280:CDS
TGAAGCTAGCAATCTGAAGA+TGG 0.529573 4_1:+45690515 Msa0550280:CDS
GTCTCTTCAAAGACAAGTTG+AGG 0.543289 4_1:+45689302 Msa0550280:CDS
CAACAACTAGTTTCATGTTG+TGG 0.545151 4_1:+45689860 Msa0550280:CDS
AGAGAAAATTGTGATGATGT+TGG 0.545309 4_1:+45688648 Msa0550280:CDS
AAACATTGAATCAAATGCAA+TGG 0.546511 4_1:-45690789 None:intergenic
ATTGAATCAAATGCAATGGA+AGG 0.547369 4_1:-45690785 None:intergenic
CTCCGGAAATTGGGGTTCGA+AGG 0.548238 4_1:+45689912 Msa0550280:CDS
CTACCTGTAAATGGTTGAGA+AGG 0.549856 4_1:-45690184 None:intergenic
CATTTCCGACATGACAGTGT+TGG 0.552355 4_1:+45688293 Msa0550280:CDS
TAACTCTATGAAACCTGACC+CGG 0.555893 4_1:+45688470 Msa0550280:CDS
CTTCCCAAGTTGAGGATGGT+AGG 0.556292 4_1:-45690105 None:intergenic
CAACTAGTTTCATGTTGTGG+TGG 0.563540 4_1:+45689863 Msa0550280:CDS
AAAATCACTGAGCAAACAAA+GGG 0.564488 4_1:+45688600 Msa0550280:CDS
GATAGTCCCTCCGGAAATTG+GGG 0.565816 4_1:+45689904 Msa0550280:CDS
ATTGCAAATTAGGGTGTTGG+TGG 0.566623 4_1:+45690759 Msa0550280:three_prime_UTR
TTGTGATGATGTTGGTTCCA+AGG 0.568124 4_1:+45688656 Msa0550280:CDS
TTGTGATTCTGCTCTTGGTG+AGG 0.569669 4_1:+45688440 Msa0550280:CDS
TCCATTGCAAATTAGGGTGT+TGG 0.573419 4_1:+45690756 Msa0550280:three_prime_UTR
ACGTGCTAAACTACGCACTT+GGG 0.576592 4_1:-45688160 None:intergenic
TCAGGTAAAGATGCAGATGC+CGG 0.578456 4_1:-45689947 None:intergenic
TCGAAGGAACATTAATGCAC+CGG 0.582886 4_1:+45689928 Msa0550280:CDS
CAAAATCACTGAGCAAACAA+AGG 0.583100 4_1:+45688599 Msa0550280:CDS
CTACCATCCTCAACTTGGGA+AGG 0.586675 4_1:+45690106 Msa0550280:CDS
ACCAACACCCTAATTTGCAA+TGG 0.594011 4_1:-45690757 None:intergenic
TATCCTACCATCCTCAACTT+GGG 0.602359 4_1:+45690102 Msa0550280:CDS
AATTGAATTGAAGATATGCA+GGG 0.606589 4_1:-45689832 None:intergenic
TCTTTCCAACACTGTCATGT+CGG 0.609762 4_1:-45688298 None:intergenic
ACTTCTGGAAATCTAGTAGG+AGG 0.609823 4_1:+45688267 Msa0550280:CDS
TCACCTTCCCAAGTTGAGGA+TGG 0.614865 4_1:-45690109 None:intergenic
CTTCGAACCCCAATTTCCGG+AGG 0.622595 4_1:-45689911 None:intergenic
TTCGAACCCCAATTTCCGGA+GGG 0.626102 4_1:-45689910 None:intergenic
TGTTGGAAAGACAAAGAGCA+AGG 0.628279 4_1:+45688310 Msa0550280:CDS
GAAAAGGAAAGCTGATAACA+AGG 0.638853 4_1:+45688521 Msa0550280:CDS
ATATAAAGTCCAAAGACAAG+AGG 0.657536 4_1:+45688547 Msa0550280:CDS
ATTCATGTTCGAGCACGTCG+CGG 0.664373 4_1:+45688723 Msa0550280:CDS
GTGGATTGATAAACAATATG+GGG 0.684555 4_1:+45689882 Msa0550280:CDS
AACTCTATGAAACCTGACCC+GGG 0.688237 4_1:+45688471 Msa0550280:CDS
CATGAACAAGATCAACAACA+CGG 0.698507 4_1:+45688363 Msa0550280:CDS
AAGGAAAGCTGATAACAAGG+TGG 0.715030 4_1:+45688524 Msa0550280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTACTAAATTGTTTAATT+TGG + chr4_1:45688984-45689003 Msa0550280:intron 10.0%
!!! TTAAGTTTAAGTTTATAATA+TGG + chr4_1:45689381-45689400 Msa0550280:intron 10.0%
!!! ATTTTATGGTTTTAGTAAAA+AGG + chr4_1:45689556-45689575 Msa0550280:intron 15.0%
!! TTAAATTATTTGATCTTTAC+AGG + chr4_1:45689766-45689785 Msa0550280:intron 15.0%
!! AATCATATTAATGATTGATT+AGG - chr4_1:45690042-45690061 None:intergenic 15.0%
!!! TTAAAGTACATAACATATTT+TGG + chr4_1:45690597-45690616 Msa0550280:three_prime_UTR 15.0%
!!! TCTCTTAATTTGTCATTATT+TGG + chr4_1:45688814-45688833 Msa0550280:intron 20.0%
!! TAGTAAAAAATGATCTAACA+CGG + chr4_1:45688885-45688904 Msa0550280:intron 20.0%
!! ATGCTAAAATTACAGTTAAT+AGG + chr4_1:45689026-45689045 Msa0550280:intron 20.0%
!!! TGATTTTTCTGTTTATTACT+CGG + chr4_1:45689131-45689150 Msa0550280:intron 20.0%
!! GTATGATAAATTAGTTACTT+AGG + chr4_1:45689336-45689355 Msa0550280:intron 20.0%
!!! AAATCTTCATTTTGATGAAA+AGG - chr4_1:45690344-45690363 None:intergenic 20.0%
!! ATGATTGAAAGTTGAAAATT+CGG + chr4_1:45690395-45690414 Msa0550280:intron 20.0%
!!! TTTCTTATTCTTTTTTCTAG+TGG - chr4_1:45690704-45690723 None:intergenic 20.0%
!!! TTGATCTTGTTGTTTGTTTT+GGG - chr4_1:45688228-45688247 None:intergenic 25.0%
! CTAAAATTACAGTTAATAGG+AGG + chr4_1:45689029-45689048 Msa0550280:intron 25.0%
!!! ACACTTATTTTGTGCAATAT+AGG + chr4_1:45689097-45689116 Msa0550280:intron 25.0%
! AATTGAATTGAAGATATGCA+GGG - chr4_1:45689835-45689854 None:intergenic 25.0%
!!! GATTAGGAACAATATTTTAG+AGG - chr4_1:45690026-45690045 None:intergenic 25.0%
!! TTTTCAAAATCTTCATAGTG+TGG + chr4_1:45690132-45690151 Msa0550280:CDS 25.0%
! AATTTGTATCTACCTGTAAA+TGG - chr4_1:45690196-45690215 None:intergenic 25.0%
!!! GAATTTTGATAAGAAGCATT+TGG - chr4_1:45690569-45690588 None:intergenic 25.0%
! AAACATTGAATCAAATGCAA+TGG - chr4_1:45690792-45690811 None:intergenic 25.0%
!!! CAATGTTTTGAAAACCAAAA+CGG + chr4_1:45690804-45690823 Msa0550280:three_prime_UTR 25.0%
!!! GTTGATCTTGTTGTTTGTTT+TGG - chr4_1:45688229-45688248 None:intergenic 30.0%
TTACATTGTCTTAACACTTC+TGG + chr4_1:45688252-45688271 Msa0550280:CDS 30.0%
! GTTCATTTTCATGTTCATGT+TGG - chr4_1:45688344-45688363 None:intergenic 30.0%
! TTCTTCACAACAGTTTTTGA+TGG + chr4_1:45688413-45688432 Msa0550280:CDS 30.0%
! ATCAGCTTTCCTTTTCTTAA+CGG - chr4_1:45688517-45688536 None:intergenic 30.0%
ATATAAAGTCCAAAGACAAG+AGG + chr4_1:45688547-45688566 Msa0550280:CDS 30.0%
AAAATCACTGAGCAAACAAA+GGG + chr4_1:45688600-45688619 Msa0550280:CDS 30.0%
! AGAGAAAATTGTGATGATGT+TGG + chr4_1:45688648-45688667 Msa0550280:CDS 30.0%
! TGAACCTTTTGAATTTTCCT+TGG - chr4_1:45688676-45688695 None:intergenic 30.0%
GTTTATTACTCGGAGTTTAA+AGG + chr4_1:45689141-45689160 Msa0550280:intron 30.0%
TATTTGCAAGATTTAGTACC+AGG + chr4_1:45689222-45689241 Msa0550280:CDS 30.0%
! GGACACAATGCAAAATTTTA+TGG + chr4_1:45689542-45689561 Msa0550280:intron 30.0%
TCTACATTTGACCGTAATTA+AGG + chr4_1:45689639-45689658 Msa0550280:intron 30.0%
! CAAAAAGCGCAAATCATTAA+TGG - chr4_1:45689721-45689740 None:intergenic 30.0%
GAATTGAATTGAAGATATGC+AGG - chr4_1:45689836-45689855 None:intergenic 30.0%
!! TGGTGGATTGATAAACAATA+TGG + chr4_1:45689880-45689899 Msa0550280:CDS 30.0%
!! GGTGGATTGATAAACAATAT+GGG + chr4_1:45689881-45689900 Msa0550280:CDS 30.0%
! GTGGATTGATAAACAATATG+GGG + chr4_1:45689882-45689901 Msa0550280:CDS 30.0%
!! AGGAACAATATTTTAGAGGA+CGG - chr4_1:45690022-45690041 None:intergenic 30.0%
AAGGACTTATCAATATCTGT+GGG - chr4_1:45690325-45690344 None:intergenic 30.0%
AAAGGACTTATCAATATCTG+TGG - chr4_1:45690326-45690345 None:intergenic 30.0%
!!! ATGTTTTGTTTTATGACTGC+AGG + chr4_1:45690486-45690505 Msa0550280:intron 30.0%
TAACTTGAATGTCCAAAAGA+AGG - chr4_1:45690673-45690692 None:intergenic 30.0%
ATCTGATCCATTGCAAATTA+GGG + chr4_1:45690750-45690769 Msa0550280:three_prime_UTR 30.0%
ATTGAATCAAATGCAATGGA+AGG - chr4_1:45690788-45690807 None:intergenic 30.0%
CATTAAACCTGCAAGAAATT+CGG + chr4_1:45690828-45690847 Msa0550280:three_prime_UTR 30.0%
! AACACTTCTGGAAATCTAGT+AGG + chr4_1:45688264-45688283 Msa0550280:CDS 35.0%
CATGAACAAGATCAACAACA+CGG + chr4_1:45688363-45688382 Msa0550280:CDS 35.0%
!! TCACAACAGTTTTTGATGGT+TGG + chr4_1:45688417-45688436 Msa0550280:CDS 35.0%
TCGAAGAAACCGTTAAGAAA+AGG + chr4_1:45688505-45688524 Msa0550280:CDS 35.0%
GAAAAGGAAAGCTGATAACA+AGG + chr4_1:45688521-45688540 Msa0550280:CDS 35.0%
!! ATCAAAGTGAGTGTTGAAGA+AGG + chr4_1:45688570-45688589 Msa0550280:CDS 35.0%
!! CTTTGTTTGCTCAGTGATTT+TGG - chr4_1:45688601-45688620 None:intergenic 35.0%
CAAAATCACTGAGCAAACAA+AGG + chr4_1:45688599-45688618 Msa0550280:CDS 35.0%
GGTTCCAAGGAAAATTCAAA+AGG + chr4_1:45688669-45688688 Msa0550280:CDS 35.0%
TATCCATCAATCATGATCGT+CGG - chr4_1:45688924-45688943 None:intergenic 35.0%
! TTGTGCAATATAGGTTAGCT+CGG + chr4_1:45689106-45689125 Msa0550280:intron 35.0%
GAAGGGAAAAAATAAGCGAA+AGG + chr4_1:45689193-45689212 Msa0550280:CDS 35.0%
TCATTGACAAGAACTGCATT+TGG - chr4_1:45689459-45689478 None:intergenic 35.0%
CAATGTTGAAGTCAAGTCTT+GGG - chr4_1:45689498-45689517 None:intergenic 35.0%
TCAATGTTGAAGTCAAGTCT+TGG - chr4_1:45689499-45689518 None:intergenic 35.0%
TGATGAATTGTTTGCCAAAG+AGG + chr4_1:45689516-45689535 Msa0550280:CDS 35.0%
AATTGTTTGCCAAAGAGGTA+TGG + chr4_1:45689521-45689540 Msa0550280:intron 35.0%
AGATGATACAGCCTTAATCA+CGG - chr4_1:45689622-45689641 None:intergenic 35.0%
GACTCATAACGCCTTAATTA+CGG - chr4_1:45689653-45689672 None:intergenic 35.0%
GACATTTCTGATTGCATCAT+TGG - chr4_1:45689809-45689828 None:intergenic 35.0%
! CAACAACTAGTTTCATGTTG+TGG + chr4_1:45689860-45689879 Msa0550280:CDS 35.0%
GATGAGTCAAGAAAGATTTC+AGG - chr4_1:45689968-45689987 None:intergenic 35.0%
!! CATAGTGTGGATTTTGATCA+AGG + chr4_1:45690145-45690164 Msa0550280:CDS 35.0%
TTTCCTTCTCAACCATTTAC+AGG + chr4_1:45690181-45690200 Msa0550280:CDS 35.0%
!! CTTTCTACTTCTAAGATTGC+AGG - chr4_1:45690249-45690268 None:intergenic 35.0%
AGGACTTATCAATATCTGTG+GGG - chr4_1:45690324-45690343 None:intergenic 35.0%
CCTTGCAAACCTTTATATTC+CGG + chr4_1:45690427-45690446 Msa0550280:intron 35.0%
CTTTATATTCCGGAAAAACG+CGG + chr4_1:45690437-45690456 Msa0550280:intron 35.0%
CAAAAGAAGGAGAATGTAAC+AGG - chr4_1:45690660-45690679 None:intergenic 35.0%
! CTGTTACATTCTCCTTCTTT+TGG + chr4_1:45690658-45690677 Msa0550280:three_prime_UTR 35.0%
CATCTGATCCATTGCAAATT+AGG + chr4_1:45690749-45690768 Msa0550280:three_prime_UTR 35.0%
CGCTATGCTCTTTGTAGAAA+TGG - chr4_1:45688124-45688143 None:intergenic 40.0%
TTCAGAGTGTGCAATGTACT+AGG - chr4_1:45688193-45688212 None:intergenic 40.0%
! ACTTCTGGAAATCTAGTAGG+AGG + chr4_1:45688267-45688286 Msa0550280:CDS 40.0%
TCTTTCCAACACTGTCATGT+CGG - chr4_1:45688301-45688320 None:intergenic 40.0%
TGTTGGAAAGACAAAGAGCA+AGG + chr4_1:45688310-45688329 Msa0550280:CDS 40.0%
!!! ACAACACGGACGTTACTTTT+CGG + chr4_1:45688377-45688396 Msa0550280:CDS 40.0%
TAACTCTATGAAACCTGACC+CGG + chr4_1:45688470-45688489 Msa0550280:CDS 40.0%
!!! TCTTCGACTTTTTCGAAACC+CGG - chr4_1:45688492-45688511 None:intergenic 40.0%
AAGGAAAGCTGATAACAAGG+TGG + chr4_1:45688524-45688543 Msa0550280:CDS 40.0%
CACTTTGATCCTCTTGTCTT+TGG - chr4_1:45688559-45688578 None:intergenic 40.0%
!! TTGTGATGATGTTGGTTCCA+AGG + chr4_1:45688656-45688675 Msa0550280:CDS 40.0%
GCTCGAACATGAATGTAATC+AGG - chr4_1:45688717-45688736 None:intergenic 40.0%
ATTCCGACGATCATGATTGA+TGG + chr4_1:45688918-45688937 Msa0550280:intron 40.0%
GCACAAAACAGAACAAACGT+TGG - chr4_1:45689167-45689186 None:intergenic 40.0%
! ACGTTTGTTCTGTTTTGTGC+AGG + chr4_1:45689167-45689186 Msa0550280:intron 40.0%
! TCTGTTTTGTGCAGGTTAGA+AGG + chr4_1:45689175-45689194 Msa0550280:intron 40.0%
! CTGTTTTGTGCAGGTTAGAA+GGG + chr4_1:45689176-45689195 Msa0550280:intron 40.0%
! CCTGCAATTTTGTTACATCC+TGG - chr4_1:45689243-45689262 None:intergenic 40.0%
CCAGGATGTAACAAAATTGC+AGG + chr4_1:45689240-45689259 Msa0550280:CDS 40.0%
AACAAAATTGCAGGCAAAGC+TGG + chr4_1:45689249-45689268 Msa0550280:CDS 40.0%
GTCTCTTCAAAGACAAGTTG+AGG + chr4_1:45689302-45689321 Msa0550280:CDS 40.0%
!! GGTTTTAGTAAAAAGGTCCG+CGG + chr4_1:45689563-45689582 Msa0550280:intron 40.0%
GGCATCAAAAATCATGATGC+TGG - chr4_1:45689589-45689608 None:intergenic 40.0%
! GCATCATGATTTTTGATGCC+AGG + chr4_1:45689589-45689608 Msa0550280:intron 40.0%
TGTTGTGATTCGCGATATTG+TGG - chr4_1:45689695-45689714 None:intergenic 40.0%
! CAACTAGTTTCATGTTGTGG+TGG + chr4_1:45689863-45689882 Msa0550280:CDS 40.0%
TCGAAGGAACATTAATGCAC+CGG + chr4_1:45689928-45689947 Msa0550280:CDS 40.0%
ATATCCTACCATCCTCAACT+TGG + chr4_1:45690101-45690120 Msa0550280:CDS 40.0%
TATCCTACCATCCTCAACTT+GGG + chr4_1:45690102-45690121 Msa0550280:CDS 40.0%
AAAATCACCTTCCCAAGTTG+AGG - chr4_1:45690116-45690135 None:intergenic 40.0%
CTACCTGTAAATGGTTGAGA+AGG - chr4_1:45690187-45690206 None:intergenic 40.0%
!! ACTTCTAAGATTGCAGGCTA+AGG - chr4_1:45690243-45690262 None:intergenic 40.0%
AAAGGTTTGCAAGGTAACCA+CGG - chr4_1:45690421-45690440 None:intergenic 40.0%
CCGGAATATAAAGGTTTGCA+AGG - chr4_1:45690430-45690449 None:intergenic 40.0%
! CGCGTTTTTCCGGAATATAA+AGG - chr4_1:45690439-45690458 None:intergenic 40.0%
TGAAGCTAGCAATCTGAAGA+TGG + chr4_1:45690515-45690534 Msa0550280:CDS 40.0%
TTGGAGTGAGCAAAAAGTTC+AGG - chr4_1:45690550-45690569 None:intergenic 40.0%
AAAGAGAGAGATTTGTGAGC+AGG + chr4_1:45690726-45690745 Msa0550280:three_prime_UTR 40.0%
TCCATTGCAAATTAGGGTGT+TGG + chr4_1:45690756-45690775 Msa0550280:three_prime_UTR 40.0%
! ACCAACACCCTAATTTGCAA+TGG - chr4_1:45690760-45690779 None:intergenic 40.0%
!! ATTGCAAATTAGGGTGTTGG+TGG + chr4_1:45690759-45690778 Msa0550280:three_prime_UTR 40.0%
! TGCAGGTTTAATGTCCGTTT+TGG - chr4_1:45690821-45690840 None:intergenic 40.0%
TCATGAACCGAATTTCTTGC+AGG - chr4_1:45690838-45690857 None:intergenic 40.0%
ACGTGCTAAACTACGCACTT+GGG - chr4_1:45688163-45688182 None:intergenic 45.0%
TACGTGCTAAACTACGCACT+TGG - chr4_1:45688164-45688183 None:intergenic 45.0%
CAACACTGTCATGTCGGAAA+TGG - chr4_1:45688295-45688314 None:intergenic 45.0%
CATTTCCGACATGACAGTGT+TGG + chr4_1:45688293-45688312 Msa0550280:CDS 45.0%
!!! CAACACGGACGTTACTTTTC+GGG + chr4_1:45688378-45688397 Msa0550280:CDS 45.0%
!!! GGACGTTACTTTTCGGGAAA+TGG + chr4_1:45688384-45688403 Msa0550280:CDS 45.0%
GTTGGTTGTGATTCTGCTCT+TGG + chr4_1:45688435-45688454 Msa0550280:CDS 45.0%
TTGTGATTCTGCTCTTGGTG+AGG + chr4_1:45688440-45688459 Msa0550280:CDS 45.0%
AACTCTATGAAACCTGACCC+GGG + chr4_1:45688471-45688490 Msa0550280:CDS 45.0%
!!! CTTCGACTTTTTCGAAACCC+GGG - chr4_1:45688491-45688510 None:intergenic 45.0%
GCATTGTGTCCATACCTCTT+TGG - chr4_1:45689533-45689552 None:intergenic 45.0%
AAAAATCATGATGCTGGCCG+CGG - chr4_1:45689583-45689602 None:intergenic 45.0%
CGCGATATTGTGGATAATCG+CGG - chr4_1:45689685-45689704 None:intergenic 45.0%
TTCCTTCGAACCCCAATTTC+CGG - chr4_1:45689917-45689936 None:intergenic 45.0%
TCAGGTAAAGATGCAGATGC+CGG - chr4_1:45689950-45689969 None:intergenic 45.0%
GAAAGTTGAAAATTCGGCCG+TGG + chr4_1:45690401-45690420 Msa0550280:intron 45.0%
! TCGAATCTACCGCGTTTTTC+CGG - chr4_1:45690449-45690468 None:intergenic 45.0%
AAAACGCGGTAGATTCGATG+CGG + chr4_1:45690451-45690470 Msa0550280:intron 45.0%
!!! ACTTTTTCGAAACCCGGGTC+AGG - chr4_1:45688486-45688505 None:intergenic 50.0%
ATTCATGTTCGAGCACGTCG+CGG + chr4_1:45688723-45688742 Msa0550280:CDS 50.0%
TACAGCCTTAATCACGGTCG+TGG - chr4_1:45689616-45689635 None:intergenic 50.0%
CAATATGGGGATAGTCCCTC+CGG + chr4_1:45689895-45689914 Msa0550280:CDS 50.0%
GGATAGTCCCTCCGGAAATT+GGG + chr4_1:45689903-45689922 Msa0550280:CDS 50.0%
GATAGTCCCTCCGGAAATTG+GGG + chr4_1:45689904-45689923 Msa0550280:CDS 50.0%
TTCGAACCCCAATTTCCGGA+GGG - chr4_1:45689913-45689932 None:intergenic 50.0%
TAGAGGACGGATACACAGAG+AGG - chr4_1:45690009-45690028 None:intergenic 50.0%
! CTTCCCAAGTTGAGGATGGT+AGG - chr4_1:45690108-45690127 None:intergenic 50.0%
CTACCATCCTCAACTTGGGA+AGG + chr4_1:45690106-45690125 Msa0550280:CDS 50.0%
! TCACCTTCCCAAGTTGAGGA+TGG - chr4_1:45690112-45690131 None:intergenic 50.0%
AAACGCGGTAGATTCGATGC+GGG + chr4_1:45690452-45690471 Msa0550280:intron 50.0%
CTTAATCACGGTCGTGGACC+TGG - chr4_1:45689610-45689629 None:intergenic 55.0%
CAGGTCCACGACCGTGATTA+AGG + chr4_1:45689608-45689627 Msa0550280:intron 55.0%
GGGATAGTCCCTCCGGAAAT+TGG + chr4_1:45689902-45689921 Msa0550280:CDS 55.0%
CTTCGAACCCCAATTTCCGG+AGG - chr4_1:45689914-45689933 None:intergenic 55.0%
CTCCGGAAATTGGGGTTCGA+AGG + chr4_1:45689912-45689931 Msa0550280:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 45688079 45690862 45688079 ID=Msa0550280;Name=Msa0550280
chr4_1 mRNA 45688079 45690862 45688079 ID=Msa0550280-mRNA-1;Parent=Msa0550280;Name=Msa0550280-mRNA-1;_AED=0.11;_eAED=0.11;_QI=170|1|1|1|1|1|6|317|362
chr4_1 exon 45688079 45688779 45688079 ID=Msa0550280-mRNA-1:exon:12091;Parent=Msa0550280-mRNA-1
chr4_1 exon 45689189 45689323 45689189 ID=Msa0550280-mRNA-1:exon:12092;Parent=Msa0550280-mRNA-1
chr4_1 exon 45689466 45689537 45689466 ID=Msa0550280-mRNA-1:exon:12093;Parent=Msa0550280-mRNA-1
chr4_1 exon 45689788 45689997 45689788 ID=Msa0550280-mRNA-1:exon:12094;Parent=Msa0550280-mRNA-1
chr4_1 exon 45690100 45690202 45690100 ID=Msa0550280-mRNA-1:exon:12095;Parent=Msa0550280-mRNA-1
chr4_1 exon 45690508 45690862 45690508 ID=Msa0550280-mRNA-1:exon:12096;Parent=Msa0550280-mRNA-1
chr4_1 five_prime_UTR 45688079 45688248 45688079 ID=Msa0550280-mRNA-1:five_prime_utr;Parent=Msa0550280-mRNA-1
chr4_1 CDS 45688249 45688779 45688249 ID=Msa0550280-mRNA-1:cds;Parent=Msa0550280-mRNA-1
chr4_1 CDS 45689189 45689323 45689189 ID=Msa0550280-mRNA-1:cds;Parent=Msa0550280-mRNA-1
chr4_1 CDS 45689466 45689537 45689466 ID=Msa0550280-mRNA-1:cds;Parent=Msa0550280-mRNA-1
chr4_1 CDS 45689788 45689997 45689788 ID=Msa0550280-mRNA-1:cds;Parent=Msa0550280-mRNA-1
chr4_1 CDS 45690100 45690202 45690100 ID=Msa0550280-mRNA-1:cds;Parent=Msa0550280-mRNA-1
chr4_1 CDS 45690508 45690545 45690508 ID=Msa0550280-mRNA-1:cds;Parent=Msa0550280-mRNA-1
chr4_1 three_prime_UTR 45690546 45690862 45690546 ID=Msa0550280-mRNA-1:three_prime_utr;Parent=Msa0550280-mRNA-1
Gene Sequence

>Msa0550280

ATGTTACATTGTCTTAACACTTCTGGAAATCTAGTAGGAGGAACCATTTCCGACATGACAGTGTTGGAAAGACAAAGAGCAAGGAAGAAGTTCCAACATGAACATGAAAATGAACATGAACAAGATCAACAACACGGACGTTACTTTTCGGGAAATGGTTTCTCTTCTTCACAACAGTTTTTGATGGTTGGTTGTGATTCTGCTCTTGGTGAGGTTGTAGCTAACTCTATGAAACCTGACCCGGGTTTCGAAAAAGTCGAAGAAACCGTTAAGAAAAGGAAAGCTGATAACAAGGTGGATATAAAGTCCAAAGACAAGAGGATCAAAGTGAGTGTTGAAGAAGGTGAATCCAAAATCACTGAGCAAACAAAGGGACAGAAGAATACAAAGTTGAAAAATAGAGAAAATTGTGATGATGTTGGTTCCAAGGAAAATTCAAAAGGTTCAGAGATTCAAAATCAAAAGCCTGATTACATTCATGTTCGAGCACGTCGCGGTCAAGCTACTGATAGTCATAGCTTAGCTGAAAGAGTTAGAAGGGAAAAAATAAGCGAAAGGATGAAGTATTTGCAAGATTTAGTACCAGGATGTAACAAAATTGCAGGCAAAGCTGGAATGCTTGATGAAATTATTAACTATGTTCAGTCTCTTCAAAGACAAGTTGAGTTCTTGTCAATGAAATTAGCAGCTGTAAACCCAAGACTTGACTTCAACATTGATGAATTGTTTGCCAAAGAGGTGTTTACTCAAAATTTTCCAATGATGCAATCAGAAATGTCTAACCCTGCATATCTTCAATTCAATTCAACACAACAACTAGTTTCATGTTGTGGTGGATTGATAAACAATATGGGGATAGTCCCTCCGGAAATTGGGGTTCGAAGGAACATTAATGCACCGGCATCTGCATCTTTACCTGAAATCTTTCTTGACTCATCATGTTTCACTCATATCCTACCATCCTCAACTTGGGAAGGTGATTTTCAAAATCTTCATAGTGTGGATTTTGATCAAGGAAGATCAACATCATTTCCTTCTCAACCATTTACAGGCATGATTGAAGCTAGCAATCTGAAGATGGAGATGTAA
Protein sequence

>Msa0550280

MLHCLNTSGNLVGGTISDMTVLERQRARKKFQHEHENEHEQDQQHGRYFSGNGFSSSQQFLMVGCDSALGEVVANSMKPDPGFEKVEETVKKRKADNKVDIKSKDKRIKVSVEEGESKITEQTKGQKNTKLKNRENCDDVGSKENSKGSEIQNQKPDYIHVRARRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKIAGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRLDFNIDELFAKEVFTQNFPMMQSEMSNPAYLQFNSTQQLVSCCGGLINNMGIVPPEIGVRRNINAPASASLPEIFLDSSCFTHILPSSTWEGDFQNLHSVDFDQGRSTSFPSQPFTGMIEASNLKMEM*