AlfalfaGEDB | Msa0618770

M. sativa cultivar zhongmu-4 / Msa0618770

KEGG: K07052
GO: -
PFAM: PF02517.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0618770	RHN43423.1	90.407	344	8	1	1	319	1	344	0.0	612

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0618770	A0A396GSC2	90.407	344	8	1	1	319	1	344	0.0	612

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa0618770	0.808746	2.661247e-50	-8.615850e-47
Msa0002110	Msa0618770	0.834764	2.481439e-56	-8.615850e-47
Msa0003340	Msa0618770	0.836384	9.658704e-57	-8.615850e-47
Msa0007810	Msa0618770	0.857996	1.115041e-62	-8.615850e-47
Msa0009560	Msa0618770	0.809491	1.843646e-50	-8.615850e-47
Msa0011060	Msa0618770	0.844366	7.903642e-59	-8.615850e-47
Msa0011100	Msa0618770	0.841579	4.362606e-58	-8.615850e-47
Msa0011440	Msa0618770	0.805676	1.189976e-49	-8.615850e-47
Msa0014970	Msa0618770	0.850320	1.833122e-60	-8.615850e-47
Msa0016380	Msa0618770	0.849358	3.404742e-60	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0618770	MtrunA17_Chr8g0387651	90.407	344	8	1	1	319	1	344	0.0	612
Msa0618770	MtrunA17_Chr1g0202351	53.503	314	94	4	29	319	30	314	1.61e-108	318
Msa0618770	MtrunA17_Chr8g0361751	26.230	305	184	7	28	315	40	320	7.54e-18	82.8
Msa0618770	MtrunA17_Chr4g0021461	29.932	147	91	2	165	311	151	285	1.55e-12	67.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0618770	AT5G60750.1	60.997	341	94	6	6	319	19	347	6.06e-131	377
Msa0618770	AT1G14270.4	45.882	85	46	0	231	315	124	208	2.56e-17	80.1
Msa0618770	AT1G14270.3	45.882	85	46	0	231	315	124	208	2.56e-17	80.1
Msa0618770	AT1G14270.2	45.882	85	46	0	231	315	144	228	4.56e-17	79.7
Msa0618770	AT1G14270.1	30.682	176	111	4	149	315	161	334	1.15e-16	80.1
Msa0618770	AT2G20725.1	26.531	294	167	10	53	315	24	299	1.06e-13	70.9

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Find 125 sgRNAs with CRISPR-Local

Find 329 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTATTTCCTCCCACAC+AGG	0.659992	4_2:+69699132	None:intergenic
AAACACAAAATCTACATAAC+AGG	0.379877	4_2:-69702989	Msa0618770:five_prime_UTR
AAACAGTGGTCTATAATTTC+CGG	0.342884	4_2:+69701969	None:intergenic
AAGGATTCTTTGACATTTAG+AGG	0.293855	4_2:-69699468	Msa0618770:CDS
AAGGATTGAAACGAGAATTT+GGG	0.367301	4_2:+69702609	None:intergenic
AATAAAAGCAAGGGATCCTG+TGG	0.652461	4_2:-69699193	Msa0618770:CDS
AATTGAGTATTCTGTGCTTT+AGG	0.300509	4_2:-69702025	Msa0618770:CDS
AATTGGTGCAATCTGAATCA+AGG	0.508090	4_2:-69701938	Msa0618770:CDS
ACACCACACAGGCATGTATT+TGG	0.285573	4_2:+69699083	None:intergenic
ACCAAATAGAGTGAATGTCC+TGG	0.576963	4_2:-69703064	Msa0618770:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATATCATGAACAAGACAAT+TGG	+	chr4_2:69698759-69698778	None:intergenic	30.0%
ATTTCTAACCCAGAAAATAG+AGG	+	chr4_2:69698842-69698861	None:intergenic	30.0%
CATCAAGGATATGGATTTAA+AGG	+	chr4_2:69699070-69699089	None:intergenic	30.0%
AAGGATTGAAACGAGAATTT+GGG	+	chr4_2:69699247-69699266	None:intergenic	30.0%
GATAAGCATTTGTGAAAGTT+TGG	+	chr4_2:69699730-69699749	None:intergenic	30.0%
TATGAAACTGTTTATCTACC+AGG	+	chr4_2:69699752-69699771	None:intergenic	30.0%
AATTGAGTATTCTGTGCTTT+AGG	-	chr4_2:69699828-69699847	Msa0618770:intron	30.0%
AAACAGTGGTCTATAATTTC+CGG	+	chr4_2:69699887-69699906	None:intergenic	30.0%
ATAATGTTGTCAAAAGAGAC+TGG	-	chr4_2:69699942-69699961	Msa0618770:intron	30.0%
TTTATCACCATCAAAACTGA+TGG	+	chr4_2:69700116-69700135	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	69698669	69703206	69698669	ID=Msa0618770;Name=Msa0618770
chr4_2	mRNA	69698669	69703206	69698669	ID=Msa0618770-mRNA-1;Parent=Msa0618770;Name=Msa0618770-mRNA-1;_AED=0.24;_eAED=0.24;_QI=471\|0.5\|1\|1\|1\|1\|3\|234\|319
chr4_2	exon	69698669	69699571	69698669	ID=Msa0618770-mRNA-1:exon:20208;Parent=Msa0618770-mRNA-1
chr4_2	exon	69701893	69702048	69701893	ID=Msa0618770-mRNA-1:exon:20207;Parent=Msa0618770-mRNA-1
chr4_2	exon	69702601	69703206	69702601	ID=Msa0618770-mRNA-1:exon:20206;Parent=Msa0618770-mRNA-1
chr4_2	five_prime_UTR	69702736	69703206	69702736	ID=Msa0618770-mRNA-1:five_prime_utr;Parent=Msa0618770-mRNA-1
chr4_2	CDS	69702601	69702735	69702601	ID=Msa0618770-mRNA-1:cds;Parent=Msa0618770-mRNA-1
chr4_2	CDS	69701893	69702048	69701893	ID=Msa0618770-mRNA-1:cds;Parent=Msa0618770-mRNA-1
chr4_2	CDS	69698903	69699571	69698903	ID=Msa0618770-mRNA-1:cds;Parent=Msa0618770-mRNA-1
chr4_2	three_prime_UTR	69698669	69698902	69698669	ID=Msa0618770-mRNA-1:three_prime_utr;Parent=Msa0618770-mRNA-1

Gene Sequence

>Msa0618770

ATGTTGTTTACTTCGTCTTCTTCTCATTCTCTATTTGCTCGTCCAATTAAGCCTTTTCCTCGTCTCCCGACACTTCGTCCTTTTCCTACCACCTCGTTTCGGATCCCAAATTCTCGTTTCAATCCTTCAAACAACAAATTGAGTATTCTGTGCTTTAGGCATGAAGAGCATAATTCTTCAGAAACCCCCAAACCGGAAATTATAGACCACTGTTTTCATGAGGAATTGGTGCAATCTGAATCAAGGGACCATAATGTTGTCAAAAGAGACTGGAAGTCAATCATTCCTAAGGTTATGCTTCTTTGGACATCGGCATTTTGGTTTATAGGATCTTGGATGATTCCTTTTGCAGCCCATATAACTGGCTTCAGCAAGGATTCTTTGACATTTAGAGGGCAGGCCCTATTTAGCCTTGTCACTGATGTAACTGAAGGACTAGCTGGAATTGCAATTCTTGTTCGTTGTCTTTCACGATTCCGTCCCCTTCCACCCGACTGGTTCAAGTTTAGCCTGGAAGGAAAATGGCATTTTGATGTTGTCATGGGTTGTCTCATGTTTCCTCTTGTCAATCGGCTCTCTCAGTTCAACCTTGACCTACTACCCATCCTGCCATCGACACAGGTCACCCTTTCAAGTGTTGAACAATCAATAAAAGCAAGGGATCCTGTGGCAATGTTGTTGTATGCAATTGTAGTATCGGTCTGCGCTCCTGTGTGGGAGGAAATAGTTTTCCGCGGTTTTCTACTCCCGTCACTTACCAAATACATGCCTGTGTGGTGTGCAATACTGGTAAGTTCAGTTGCGTTTGCGCTTGCACATTTTAACATACAGAGGATGCTGCCACTTATATTTCTTGGGATGGTGATGGGTGTGATATTTACACGGTCAAGGAATTTATTGCCATCAATGTTGTTGCATAGTCTTTGGAATGGCTTTGTCTTTTTAGATTTGATGAAATAG

Protein sequence

>Msa0618770

MLFTSSSSHSLFARPIKPFPRLPTLRPFPTTSFRIPNSRFNPSNNKLSILCFRHEEHNSSETPKPEIIDHCFHEELVQSESRDHNVVKRDWKSIIPKVMLLWTSAFWFIGSWMIPFAAHITGFSKDSLTFRGQALFSLVTDVTEGLAGIAILVRCLSRFRPLPPDWFKFSLEGKWHFDVVMGCLMFPLVNRLSQFNLDLLPILPSTQVTLSSVEQSIKARDPVAMLLYAIVVSVCAPVWEEIVFRGFLLPSLTKYMPVWCAILVSSVAFALAHFNIQRMLPLIFLGMVMGVIFTRSRNLLPSMLLHSLWNGFVFLDLMK*