Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0074910 | sp|P50053|KHK_HUMAN | 26.163 | 344 | 189 | 11 | 49 | 383 | 2 | 289 | 4.57e-18 | 87.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0074910 | A0A396JZJ8 | 92.421 | 409 | 21 | 3 | 1 | 405 | 64 | 466 | 0.0 | 741 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000220 | Msa0074910 | 0.819548 | 1.096135e-52 | -8.615850e-47 |
| Msa0001510 | Msa0074910 | 0.893224 | 7.667104e-75 | -8.615850e-47 |
| Msa0001540 | Msa0074910 | 0.853239 | 2.725077e-61 | -8.615850e-47 |
| Msa0002110 | Msa0074910 | 0.809989 | 1.440291e-50 | -8.615850e-47 |
| Msa0002690 | Msa0074910 | 0.868160 | 8.026344e-66 | -8.615850e-47 |
| Msa0002700 | Msa0074910 | 0.858400 | 8.453802e-63 | -8.615850e-47 |
| Msa0003340 | Msa0074910 | 0.814813 | 1.272551e-51 | -8.615850e-47 |
| Msa0004180 | Msa0074910 | 0.873964 | 9.776782e-68 | -8.615850e-47 |
| Msa0007570 | Msa0074910 | 0.864588 | 1.091444e-64 | -8.615850e-47 |
| Msa0009210 | Msa0074910 | 0.818606 | 1.795908e-52 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0074910 | MtrunA17_Chr1g0183021 | 92.421 | 409 | 21 | 3 | 1 | 405 | 64 | 466 | 0.0 | 741 |
| Msa0074910 | MtrunA17_Chr7g0262551 | 73.889 | 360 | 94 | 0 | 46 | 405 | 5 | 364 | 0.0 | 565 |
| Msa0074910 | MtrunA17_Chr7g0262571 | 62.532 | 395 | 148 | 0 | 10 | 404 | 24 | 418 | 0.0 | 517 |
| Msa0074910 | MtrunA17_Chr1g0183031 | 65.833 | 360 | 123 | 0 | 46 | 405 | 9 | 368 | 1.80e-176 | 496 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0074910 | AT4G28706.3 | 67.042 | 355 | 117 | 0 | 50 | 404 | 48 | 402 | 0.0 | 512 |
| Msa0074910 | AT4G28706.1 | 67.042 | 355 | 117 | 0 | 50 | 404 | 46 | 400 | 0.0 | 512 |
| Msa0074910 | AT4G28706.4 | 67.135 | 356 | 116 | 1 | 50 | 404 | 48 | 403 | 0.0 | 511 |
| Msa0074910 | AT4G28706.2 | 67.135 | 356 | 116 | 1 | 50 | 404 | 48 | 403 | 0.0 | 511 |
| Msa0074910 | AT5G43910.2 | 54.722 | 360 | 158 | 2 | 46 | 404 | 8 | 363 | 5.99e-140 | 404 |
| Msa0074910 | AT5G43910.4 | 55.031 | 318 | 138 | 2 | 46 | 362 | 8 | 321 | 1.12e-121 | 356 |
| Msa0074910 | AT5G43910.1 | 54.015 | 274 | 125 | 1 | 46 | 318 | 8 | 281 | 5.61e-104 | 309 |
| Msa0074910 | AT5G43910.5 | 54.815 | 270 | 121 | 1 | 46 | 314 | 8 | 277 | 6.30e-104 | 310 |
| Msa0074910 | AT5G43910.3 | 54.815 | 270 | 121 | 1 | 46 | 314 | 8 | 277 | 1.15e-103 | 308 |
Find 123 sgRNAs with CRISPR-Local
Find 386 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTGCAGAGATTCGACA+TGG | 0.688208 | 1_2:+49965221 | None:intergenic |
| AAAATTTGTGGCTGTAACTT+TGG | 0.249227 | 1_2:-49961137 | Msa0074910:CDS |
| AAACACAAGGCTCGTTTCTA+AGG | 0.332845 | 1_2:-49964421 | Msa0074910:intron |
| AAAGGAAGCAACCTTCAAAG+TGG | 0.648978 | 1_2:+49964797 | None:intergenic |
| AAAGGCAGAAGGAATAGGAA+CGG | 0.658683 | 1_2:-49960436 | Msa0074910:CDS |
| AAATGCATCCCCGGCACCAG+TGG | 0.595424 | 1_2:+49960353 | None:intergenic |
| AAATTAAAGGCAGAAGGAAT+AGG | 0.458558 | 1_2:-49960441 | Msa0074910:CDS |
| AAATTTGTGGCTGTAACTTT+GGG | 0.365260 | 1_2:-49961136 | Msa0074910:CDS |
| AACGGTAGCCAAGAAATCGA+CGG | 0.675256 | 1_2:+49964857 | None:intergenic |
| AACGGTGGCGCTTTGAGGAA+CGG | 0.543705 | 1_2:+49964993 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAACCTCAAAATTTGGC+GGG | + | chr1_2:49959916-49959935 | None:intergenic | 30.0% | |
| AAGCTAGTATAGTGCATATT+TGG | - | chr1_2:49960093-49960112 | Msa0074910:intron | 30.0% | |
| TTAAATAAGCAATGTCGAGA+GGG | + | chr1_2:49960472-49960491 | None:intergenic | 30.0% | |
| ATTAAATAAGCAATGTCGAG+AGG | + | chr1_2:49960473-49960492 | None:intergenic | 30.0% | |
| CACTTAAACAAGTGGTTTAT+CGG | + | chr1_2:49960546-49960565 | None:intergenic | 30.0% | |
| CTTCATGAAAGCTTGAAATT+TGG | - | chr1_2:49960977-49960996 | Msa0074910:CDS | 30.0% | |
| TATTGTTGCCCATAACTAAA+AGG | + | chr1_2:49961051-49961070 | None:intergenic | 30.0% | |
| AAAATACTGAGATTTGAGAG+TGG | + | chr1_2:49961149-49961168 | None:intergenic | 30.0% | |
| ATTCGAAGTATAATTTCCCA+AGG | - | chr1_2:49961190-49961209 | Msa0074910:CDS | 30.0% | |
| TTCGAAGTATAATTTCCCAA+GGG | - | chr1_2:49961191-49961210 | Msa0074910:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 49959513 | 49965358 | 49959513 | ID=Msa0074910;Name=Msa0074910 |
| chr1_2 | mRNA | 49959513 | 49965358 | 49959513 | ID=Msa0074910-mRNA-1;Parent=Msa0074910;Name=Msa0074910-mRNA-1;_AED=0.33;_eAED=0.33;_QI=117|1|1|1|1|1|13|302|405 |
| chr1_2 | exon | 49959513 | 49959898 | 49959513 | ID=Msa0074910-mRNA-1:exon:15332;Parent=Msa0074910-mRNA-1 |
| chr1_2 | exon | 49960145 | 49960197 | 49960145 | ID=Msa0074910-mRNA-1:exon:15331;Parent=Msa0074910-mRNA-1 |
| chr1_2 | exon | 49960334 | 49960472 | 49960334 | ID=Msa0074910-mRNA-1:exon:15330;Parent=Msa0074910-mRNA-1 |
| chr1_2 | exon | 49960909 | 49961006 | 49960909 | ID=Msa0074910-mRNA-1:exon:15329;Parent=Msa0074910-mRNA-1 |
| chr1_2 | exon | 49961098 | 49961224 | 49961098 | ID=Msa0074910-mRNA-1:exon:15328;Parent=Msa0074910-mRNA-1 |
| chr1_2 | exon | 49961713 | 49961823 | 49961713 | ID=Msa0074910-mRNA-1:exon:15327;Parent=Msa0074910-mRNA-1 |
| chr1_2 | exon | 49962518 | 49962668 | 49962518 | ID=Msa0074910-mRNA-1:exon:15326;Parent=Msa0074910-mRNA-1 |
| chr1_2 | exon | 49963430 | 49963482 | 49963430 | ID=Msa0074910-mRNA-1:exon:15325;Parent=Msa0074910-mRNA-1 |
| chr1_2 | exon | 49963938 | 49964012 | 49963938 | ID=Msa0074910-mRNA-1:exon:15324;Parent=Msa0074910-mRNA-1 |
| chr1_2 | exon | 49964422 | 49964499 | 49964422 | ID=Msa0074910-mRNA-1:exon:15323;Parent=Msa0074910-mRNA-1 |
| chr1_2 | exon | 49964809 | 49964898 | 49964809 | ID=Msa0074910-mRNA-1:exon:15322;Parent=Msa0074910-mRNA-1 |
| chr1_2 | exon | 49964993 | 49965064 | 49964993 | ID=Msa0074910-mRNA-1:exon:15321;Parent=Msa0074910-mRNA-1 |
| chr1_2 | exon | 49965155 | 49965358 | 49965155 | ID=Msa0074910-mRNA-1:exon:15320;Parent=Msa0074910-mRNA-1 |
| chr1_2 | five_prime_UTR | 49965242 | 49965358 | 49965242 | ID=Msa0074910-mRNA-1:five_prime_utr;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49965155 | 49965241 | 49965155 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49964993 | 49965064 | 49964993 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49964809 | 49964898 | 49964809 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49964422 | 49964499 | 49964422 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49963938 | 49964012 | 49963938 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49963430 | 49963482 | 49963430 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49962518 | 49962668 | 49962518 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49961713 | 49961823 | 49961713 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49961098 | 49961224 | 49961098 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49960909 | 49961006 | 49960909 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49960334 | 49960472 | 49960334 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49960145 | 49960197 | 49960145 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | CDS | 49959815 | 49959898 | 49959815 | ID=Msa0074910-mRNA-1:cds;Parent=Msa0074910-mRNA-1 |
| chr1_2 | three_prime_UTR | 49959513 | 49959814 | 49959513 | ID=Msa0074910-mRNA-1:three_prime_utr;Parent=Msa0074910-mRNA-1 |
| Gene Sequence |
>Msa0074910 ATGTCGAATCTCTGCACTTTTTCTTCTAATATTTCTTCTTCTTCAACTTTCATCTTCAACAATATTCAACATCTCAAATTCTACAATTTAACAAGGAATCATCGCAGTAGAAATTTGAAGGTCAAAATGTGCTCCTCCGTTCCTCAAAGCGCCACCGTTGTCGGTTGTGGATCAGTCACCGTCGATTTCTTGGCTACCGTTGCTGCTTATCCTAAGCCAGATGATAAAATCAGAACAACCACTTTGAAGGTTCAAGGTGGTGGTAATGCCGGCAATGCTTTAACCTGCCTTGCTCGTTTAGGTCTAAACACAAGGCTCGTTTCTAAGATTGCGGATGACAGCCCAGGCAGGGGCATATTAGATGAGCTACGGACTGATGGCGTGGATACATCTTTTATTGTGGTATCTAAAGAGGGAACTTCACCGTTTACGTACATTATTGTGGACAATGAAACGAAGACTCGAACTTGTATACATACCCCTGGATATCCTCCAATGATACCCCAAGATCTTTCAGAATCGAGTTTGTTATCTGCATTGGATGGAGCAAGCATTGTATATTTTGACGGGAGATTGTATGAAACTGCTCTAGTTGTTGCCCACGAGGCAGCTAGGAAGAATATACCCATCTTAATTGATGCAGAAAGGCCAAGGGAAGGGTTAGATGATCTCCTGAAATTAGCTGATTATGTTGTATGCTCAGCTAAGTTTCCAAAGGCATGGACAGAGGCATCAACTGTTCCGCGAGCGCTTGTTTCTATCCTTTTAAGGTTACCCAACGTAAAATTTGTGGCTGTAACTTTGGGAAAAGATGGTTGTATACTGCTCGAGAGAAGTGTCAGTGCAAGTCCTACCACAGAAGAGGTGGATGTAGACAACTTATTGGAATCGCTGGAGTTAAGAAAGGATAAGAGTGCATCAATTCCAACCTGCATATCATCGTCAGTGGCAAAATTAAAGGCAGAAGGAATAGGAACGGTTATCGGGAAACTATACATCGGTACAGCTGAGAGCATTCCGCCCTCGGAGCTTGTTGATACCACTGGTGCCGGGGATGCATTTATTGGATCTGTGATCTATGCTATTTGTTCGAAGTTCTCACCAGAGACAATGCTACCCTTTGCTGCTAATGTGGCAGGTGCCAAGTGCAGAGATCTAGGAGCACGAAGCGGTCTTCCATACCGCGCAGACCCACGCCTAGCGTCCTTTATTCAGTAG |
| Protein sequence |
>Msa0074910 MSNLCTFSSNISSSSTFIFNNIQHLKFYNLTRNHRSRNLKVKMCSSVPQSATVVGCGSVTVDFLATVAAYPKPDDKIRTTTLKVQGGGNAGNALTCLARLGLNTRLVSKIADDSPGRGILDELRTDGVDTSFIVVSKEGTSPFTYIIVDNETKTRTCIHTPGYPPMIPQDLSESSLLSALDGASIVYFDGRLYETALVVAHEAARKNIPILIDAERPREGLDDLLKLADYVVCSAKFPKAWTEASTVPRALVSILLRLPNVKFVAVTLGKDGCILLERSVSASPTTEEVDVDNLLESLELRKDKSASIPTCISSSVAKLKAEGIGTVIGKLYIGTAESIPPSELVDTTGAGDAFIGSVIYAICSKFSPETMLPFAANVAGAKCRDLGARSGLPYRADPRLASFIQ* |
