AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0703820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0703820 MtrunA17_Chr8g0382231 98.913 368 4 0 1 368 1 368 0.0 760
Msa0703820 MtrunA17_Chr4g0050021 44.327 379 161 7 27 360 18 391 2.94e-104 321
Msa0703820 MtrunA17_Chr2g0279481 43.000 400 174 7 13 362 3 398 1.24e-102 317
Msa0703820 MtrunA17_Chr1g0191711 37.685 337 185 6 24 356 29 344 1.03e-75 243
Msa0703820 MtrunA17_Chr5g0401451 33.880 366 195 7 19 363 10 349 2.87e-65 218
Msa0703820 MtrunA17_Chr2g0303541 33.429 350 197 5 37 359 10 350 1.21e-62 211
Msa0703820 MtrunA17_Chr4g0053841 35.843 332 166 7 52 359 13 321 2.90e-61 207
Msa0703820 MtrunA17_Chr8g0367891 35.463 313 169 4 55 356 29 319 1.44e-59 202
Msa0703820 MtrunA17_Chr2g0320691 31.105 344 203 5 44 361 15 350 1.54e-59 203
Msa0703820 MtrunA17_Chr1g0181181 33.229 319 200 5 41 357 15 322 1.78e-59 201
Msa0703820 MtrunA17_Chr4g0010091 31.198 359 215 5 20 363 2 343 1.19e-58 200
Msa0703820 MtrunA17_Chr8g0374011 34.167 360 174 8 47 359 15 358 7.79e-58 199
Msa0703820 MtrunA17_Chr2g0285511 33.735 332 179 7 52 357 26 342 3.40e-55 191
Msa0703820 MtrunA17_Chr1g0195911 33.033 333 183 7 52 368 36 344 6.04e-55 190
Msa0703820 MtrunA17_Chr3g0108331 34.169 319 181 7 52 364 24 319 6.27e-55 189
Msa0703820 MtrunA17_Chr4g0076521 33.134 335 194 6 45 365 19 337 1.02e-54 189
Msa0703820 MtrunA17_Chr1g0150191 31.124 347 181 8 46 362 21 339 1.86e-54 189
Msa0703820 MtrunA17_Chr5g0432861 34.286 315 194 4 52 364 25 328 5.34e-54 187
Msa0703820 MtrunA17_Chr7g0245201 33.731 335 194 6 42 362 51 371 1.78e-53 186
Msa0703820 MtrunA17_Chr1g0207461 32.849 344 173 11 42 356 18 332 1.81e-53 186
Msa0703820 MtrunA17_Chr2g0290741 32.743 339 188 8 35 354 8 325 2.64e-52 182
Msa0703820 MtrunA17_Chr7g0268781 33.333 327 170 7 52 357 36 335 3.26e-52 183
Msa0703820 MtrunA17_Chr5g0401341 30.946 349 183 8 38 356 11 331 6.69e-52 182
Msa0703820 MtrunA17_Chr5g0412591 32.059 340 169 7 42 357 16 317 8.16e-48 171
Msa0703820 MtrunA17_Chr1g0199631 31.410 312 171 5 70 357 8 300 1.14e-47 170
Msa0703820 MtrunA17_Chr1g0206621 37.324 142 78 2 59 194 75 211 3.50e-27 107
Msa0703820 MtrunA17_Chr4g0003891 31.973 147 86 1 215 361 46 178 1.69e-22 98.2
Msa0703820 MtrunA17_Chr4g0037661 23.585 318 161 7 95 362 49 334 3.35e-20 92.0
Msa0703820 MtrunA17_Chr2g0332281 33.333 147 86 2 215 361 24 158 2.77e-19 88.6
Msa0703820 MtrunA17_Chr1g0163311 22.928 362 188 13 55 362 6 330 1.06e-16 81.3
Msa0703820 MtrunA17_Chr4g0023801 21.875 288 166 7 97 357 50 305 2.07e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0703820 AT5G10250.1 58.286 350 144 2 22 369 19 368 1.60e-144 422
Msa0703820 AT5G10250.2 58.453 349 143 2 22 368 19 367 1.03e-143 421
Msa0703820 AT5G64330.2 44.828 261 97 4 44 262 44 299 1.13e-72 239
Msa0703820 AT5G64330.2 59.722 72 29 0 293 364 371 442 1.55e-19 90.9
Msa0703820 AT5G64330.1 44.828 261 97 4 44 262 44 299 3.31e-72 239
Msa0703820 AT5G64330.1 59.722 72 29 0 293 364 371 442 1.41e-19 90.9
Msa0703820 AT5G64330.3 45.763 236 81 4 69 262 10 240 3.24e-65 218
Msa0703820 AT5G64330.3 59.722 72 29 0 293 364 312 383 1.60e-19 90.5
Msa0703820 AT3G49970.1 35.922 309 174 5 60 368 2 286 8.85e-64 212
Msa0703820 AT3G49970.2 35.922 309 174 5 60 368 2 286 8.85e-64 212
Msa0703820 AT5G67385.1 35.045 331 182 5 52 367 25 337 9.83e-64 214
Msa0703820 AT5G67440.3 33.626 342 178 7 46 364 21 336 5.81e-61 206
Msa0703820 AT5G67440.1 33.626 342 178 7 46 364 21 336 5.81e-61 206
Msa0703820 AT5G48800.1 32.759 348 183 3 34 363 23 337 7.01e-60 204
Msa0703820 AT5G03250.1 34.328 335 173 6 54 357 28 346 1.35e-58 200
Msa0703820 AT1G30440.1 30.814 344 199 5 47 361 21 354 3.64e-58 200
Msa0703820 AT2G47860.4 33.750 320 174 5 52 357 37 332 1.96e-56 191
Msa0703820 AT2G30520.1 33.866 313 192 5 52 364 30 327 2.00e-56 194
Msa0703820 AT2G47860.1 33.750 320 174 5 52 357 37 332 7.92e-56 193
Msa0703820 AT5G67385.4 35.161 310 168 5 73 367 1 292 8.26e-56 192
Msa0703820 AT5G67385.5 35.161 310 168 5 73 367 1 292 8.26e-56 192
Msa0703820 AT5G67385.2 35.161 310 168 5 73 367 1 292 8.26e-56 192
Msa0703820 AT5G67385.3 35.161 310 168 5 73 367 1 292 8.26e-56 192
Msa0703820 AT5G67440.2 33.230 322 166 7 66 364 4 299 1.17e-54 188
Msa0703820 AT3G08570.1 33.639 327 180 7 52 362 34 339 2.67e-54 189
Msa0703820 AT1G03010.2 35.015 337 175 8 52 367 9 322 2.82e-54 189
Msa0703820 AT1G03010.1 34.234 333 183 6 52 367 36 349 5.96e-54 188
Msa0703820 AT1G03010.3 35.015 337 175 8 52 367 35 348 6.00e-54 188
Msa0703820 AT1G67900.5 34.018 341 178 6 42 357 16 334 2.83e-53 186
Msa0703820 AT1G67900.1 34.018 341 178 6 42 357 16 334 2.83e-53 186
Msa0703820 AT1G67900.3 34.018 341 178 6 42 357 16 334 2.83e-53 186
Msa0703820 AT1G67900.6 34.018 341 178 6 42 357 16 334 2.83e-53 186
Msa0703820 AT1G67900.2 34.018 341 178 6 42 357 16 334 2.83e-53 186
Msa0703820 AT1G67900.4 34.018 341 178 6 42 357 16 334 2.83e-53 186
Msa0703820 AT2G47860.6 33.038 339 174 7 52 357 37 355 3.82e-53 182
Msa0703820 AT3G26490.2 33.146 356 172 7 43 363 17 341 5.44e-53 183
Msa0703820 AT3G44820.1 30.548 347 204 6 47 361 18 359 1.34e-52 185
Msa0703820 AT3G26490.1 33.146 356 172 7 43 363 17 341 1.88e-52 183
Msa0703820 AT3G26490.3 33.146 356 172 7 43 363 17 341 1.88e-52 183
Msa0703820 AT3G26490.4 33.146 356 172 7 43 363 17 341 1.88e-52 183
Msa0703820 AT2G47860.3 33.038 339 174 7 52 357 37 355 2.04e-52 184
Msa0703820 AT2G14820.2 31.624 351 185 8 38 361 11 333 3.47e-52 183
Msa0703820 AT2G14820.1 31.624 351 185 8 38 361 11 333 3.47e-52 183
Msa0703820 AT5G13600.1 31.549 355 188 9 44 364 18 351 8.48e-52 182
Msa0703820 AT3G50840.1 31.212 330 195 3 47 359 11 325 1.55e-50 178
Msa0703820 AT4G31820.1 30.769 338 181 8 46 356 21 332 4.37e-50 177
Msa0703820 AT5G47800.3 31.250 336 196 8 43 362 17 333 4.89e-50 176
Msa0703820 AT5G47800.1 31.250 336 196 8 43 362 17 333 4.89e-50 176
Msa0703820 AT5G47800.4 31.250 336 196 8 43 362 17 333 4.89e-50 176
Msa0703820 AT2G30520.2 33.447 293 180 5 72 364 2 279 3.07e-49 174
Msa0703820 AT2G30520.3 33.447 293 180 5 72 364 2 279 3.07e-49 174
Msa0703820 AT5G47800.2 31.250 336 196 8 43 362 130 446 3.59e-49 176
Msa0703820 AT4G37590.1 30.312 353 183 9 38 356 11 334 5.74e-49 174
Msa0703820 AT3G08660.2 30.938 320 180 6 52 361 34 322 2.46e-47 166
Msa0703820 AT3G08660.1 30.938 320 180 6 52 361 34 322 1.21e-46 167
Msa0703820 AT5G66560.2 28.827 392 181 8 47 359 14 386 5.70e-46 164
Msa0703820 AT5G66560.1 28.827 392 181 8 47 359 14 386 5.65e-45 164
Msa0703820 AT2G23050.1 28.783 337 191 7 41 356 16 324 1.02e-42 155
Msa0703820 AT4G31820.2 31.930 285 142 7 99 356 20 279 1.24e-42 155
Msa0703820 AT3G50840.2 31.915 282 160 3 95 359 23 289 2.45e-42 155
Msa0703820 AT3G50840.3 31.915 282 160 3 95 359 23 289 2.45e-42 155
Msa0703820 AT3G08660.3 31.338 284 156 4 88 361 8 262 4.04e-42 154
Msa0703820 AT5G66560.4 31.894 301 150 6 95 359 66 347 5.10e-42 155
Msa0703820 AT5G66560.3 31.894 301 150 6 95 359 76 357 5.75e-42 155
Msa0703820 AT5G48130.1 34.071 226 120 4 52 254 39 258 1.09e-35 137
Msa0703820 AT5G03250.2 31.200 250 126 5 138 357 1 234 3.10e-31 124
Msa0703820 AT2G47860.5 31.197 234 127 4 138 357 1 214 2.72e-28 115
Msa0703820 AT2G47860.2 31.197 234 127 4 138 357 1 214 2.72e-28 115
Msa0703820 AT3G19850.1 26.603 312 159 6 94 366 51 331 4.47e-26 109
Msa0703820 AT1G52770.1 31.361 169 102 2 197 361 10 168 4.72e-23 100
Msa0703820 AT3G15570.1 30.769 156 84 3 215 359 29 171 4.06e-19 89.0
Msa0703820 AT2G23050.2 25.847 236 130 6 138 356 1 208 1.22e-18 86.7
Msa0703820 AT1G50280.1 25.338 296 165 7 95 356 51 324 6.83e-17 82.4
Msa0703820 AT1G50280.2 25.338 296 165 7 95 356 18 291 7.14e-17 82.4
Msa0703820 AT3G22104.1 24.051 316 166 11 76 357 26 301 9.89e-16 79.0
Msa0703820 AT5G17580.2 24.556 338 196 9 42 356 10 311 1.72e-15 78.2
Msa0703820 AT5G17580.1 24.847 326 186 9 54 356 7 296 9.76e-15 75.9
Msa0703820 AT3G03510.1 24.603 252 150 7 107 356 8 221 4.44e-12 67.4

Find 143 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTGTCAGACTGACTTTC+AGG 0.132980 4_4:-75860614 None:intergenic
CACAGCATCTTCTAACAATT+TGG 0.179939 4_4:-75861832 None:intergenic
CTCCAGCCTGATAGATTCTT+TGG 0.216318 4_4:-75864158 None:intergenic
GAGGAGGAATTATGCTTATT+AGG 0.225056 4_4:-75862186 None:intergenic
TGATACCTTGTGCACATTAT+AGG 0.232562 4_4:-75860879 None:intergenic
ATTCAATATTAGTAGGCTTT+TGG 0.241298 4_4:+75862677 Msa0703820:three_prime_UTR
AAGATAGAAATTAGTGTCTT+TGG 0.247653 4_4:-75862502 None:intergenic
CATTTGTCGTGCTTTCTTCA+TGG 0.250887 4_4:+75861750 Msa0703820:CDS
CAGCCTCTAATCTCAAATTC+TGG 0.250919 4_4:+75861536 Msa0703820:CDS
TCTGGTTTCATATATCATTT+AGG 0.251520 4_4:+75860499 Msa0703820:five_prime_UTR
TTAGATATAATATAAAATTT+AGG 0.251980 4_4:-75864379 None:intergenic
TGCGGATAATTTCAGCAATT+AGG 0.253941 4_4:+75861966 Msa0703820:CDS
TGCAGAGTCTTCTACGTTCT+TGG 0.264760 4_4:-75862958 None:intergenic
AACATTGTAATTGAAAGATT+TGG 0.268935 4_4:-75862729 None:intergenic
TTCCCAGAATTTGAGATTAG+AGG 0.277466 4_4:-75861539 None:intergenic
AAGCTGGTGGTTCGATGATT+TGG 0.289085 4_4:+75861919 Msa0703820:CDS
GACTTTCAGGGTCTTGTGCT+TGG 0.289224 4_4:-75860601 None:intergenic
GCGGATAATTTCAGCAATTA+GGG 0.291903 4_4:+75861967 Msa0703820:CDS
TTAGAAGGGCTTAGAGGATA+TGG 0.292438 4_4:+75862070 Msa0703820:CDS
CTCAAGCTTGAAAACTTTCC+AGG 0.304064 4_4:+75861566 Msa0703820:CDS
GCACATTATAGGTGGTTTCC+TGG 0.315175 4_4:-75860868 None:intergenic
TTCTCCTGTCATATTGGAAT+TGG 0.317604 4_4:+75862412 Msa0703820:three_prime_UTR
TATCAGGCTGGAGTTTAAAT+TGG 0.321879 4_4:+75864168 Msa0703820:three_prime_UTR
TGTCTATAGTTTCAGATGTC+TGG 0.334509 4_4:-75864025 None:intergenic
ACCTGGACAACTGTTCTTAG+AGG 0.338185 4_4:-75863046 None:intergenic
ACTTTCAGGGTCTTGTGCTT+GGG 0.351122 4_4:-75860600 None:intergenic
TTCTACGTTCTTGGTCAGTT+AGG 0.355243 4_4:-75862949 None:intergenic
CCTACTAATATTGAATTTCC+CGG 0.364748 4_4:-75862670 None:intergenic
ACAGTACTCAGCATTAATTT+AGG 0.366320 4_4:+75860469 Msa0703820:five_prime_UTR
GAGGAGTTAGAAGGGCTTAG+AGG 0.370662 4_4:+75862064 Msa0703820:CDS
AAATTTAGGCTATATGCTTC+AGG 0.374622 4_4:-75864365 None:intergenic
ACAATTCTAAAATTCTGTTA+TGG 0.376842 4_4:+75861608 Msa0703820:CDS
CCGGGAAATTCAATATTAGT+AGG 0.395757 4_4:+75862670 Msa0703820:three_prime_UTR
TTTACCAATGATCTCTGGTT+TGG 0.398294 4_4:-75861999 None:intergenic
GATGCTGCATCCTCACTTGC+GGG 0.402710 4_4:-75861887 None:intergenic
GACAAACCAAAGAATCTATC+AGG 0.413587 4_4:+75864152 Msa0703820:three_prime_UTR
ACACGGAGAGCCTTCTTCTC+GGG 0.418325 4_4:-75864292 None:intergenic
GAGCTAATTGCTGCAAGCTT+GGG 0.418641 4_4:-75860548 None:intergenic
TCTAACTCCTCATACATTCC+TGG 0.422868 4_4:-75862052 None:intergenic
GACCTTTAGGTAGGTGTCAA+TGG 0.427446 4_4:-75862814 None:intergenic
TTAATTCCTAGATATCAAAA+TGG 0.429080 4_4:+75862346 Msa0703820:CDS
AGCCTCTAATCTCAAATTCT+GGG 0.431924 4_4:+75861537 Msa0703820:CDS
ATTAATTTAGGCAGCGTGTC+TGG 0.435511 4_4:+75860481 Msa0703820:five_prime_UTR
GACACGGAGAGCCTTCTTCT+CGG 0.443418 4_4:-75864293 None:intergenic
TTATCCGAGTTGAAGTCTAT+AGG 0.445995 4_4:-75861635 None:intergenic
TATCCGAGTTGAAGTCTATA+GGG 0.446756 4_4:-75861634 None:intergenic
CTTGAATTGAAAAGTCTGTT+GGG 0.449484 4_4:-75860844 None:intergenic
TTCTGTCAGACTGACTTTCA+GGG 0.449988 4_4:-75860613 None:intergenic
TCCTTCCATGTAAAACCAAC+AGG 0.451380 4_4:+75864206 Msa0703820:three_prime_UTR
TCTACGTTCTTGGTCAGTTA+GGG 0.451610 4_4:-75862948 None:intergenic
ACGCAACTGCAACAAAAGTC+CGG 0.452080 4_4:+75862651 Msa0703820:three_prime_UTR
ATACATTTACCAATGATCTC+TGG 0.452219 4_4:-75862004 None:intergenic
AAGTGGAATCAACTCTCAAC+AGG 0.453660 4_4:+75863995 Msa0703820:three_prime_UTR
TGAGCTAATTGCTGCAAGCT+TGG 0.454672 4_4:-75860549 None:intergenic
TCTCTAGTTTCACCTCCTGT+TGG 0.456759 4_4:-75864221 None:intergenic
GGAATGTATGAGGAGTTAGA+AGG 0.465676 4_4:+75862055 Msa0703820:CDS
AGCTATTGTGACATGTTTGT+TGG 0.475232 4_4:-75860669 None:intergenic
TGAAATGATCGAGTATGTTC+TGG 0.476508 4_4:-75862774 None:intergenic
ACTTGAATTGAAAAGTCTGT+TGG 0.476835 4_4:-75860845 None:intergenic
CTTGGTCAGTTAGGGAAGGA+TGG 0.477881 4_4:-75862940 None:intergenic
ATTTCAGCAATTAGGGCAAA+GGG 0.478066 4_4:+75861974 Msa0703820:CDS
ATATCAAAATGCGGCTACAT+AGG 0.478687 4_4:+75861500 Msa0703820:CDS
ATCTGCAACAAGAACTTACA+TGG 0.493501 4_4:-75862223 None:intergenic
GCCTCTAAGAACAGTTGTCC+AGG 0.494899 4_4:+75863045 Msa0703820:three_prime_UTR
GCTATTGTGACATGTTTGTT+GGG 0.495372 4_4:-75860668 None:intergenic
ACGAGTGTGGTTTGGTGATG+TGG 0.497567 4_4:-75864258 None:intergenic
TTGGTCAGTTAGGGAAGGAT+GGG 0.499514 4_4:-75862939 None:intergenic
GATGCTGTGATTCTATAGCT+TGG 0.503818 4_4:+75861846 Msa0703820:CDS
GGAACCAAACCAGAGATCAT+TGG 0.508422 4_4:+75861995 Msa0703820:CDS
CATTCCTGGTAGGCATCTCT+TGG 0.509319 4_4:-75862038 None:intergenic
GATCTACGACGAGTGTGGTT+TGG 0.513023 4_4:-75864266 None:intergenic
CTTGTTGCAGATGCTAAAGA+TGG 0.513993 4_4:+75862234 Msa0703820:CDS
GAAAAGTCTGTTGGGATTCG+AGG 0.514401 4_4:-75860836 None:intergenic
AGCAATGCAAGAGAAGGAAC+CGG 0.515645 4_4:+75863968 Msa0703820:three_prime_UTR
CTGTGATTCTATAGCTTGGA+AGG 0.517789 4_4:+75861850 Msa0703820:CDS
TCAGACCTTGAAAAGAGAGT+TGG 0.522254 4_4:+75862289 Msa0703820:CDS
GTGTAGAATTGTAGTTAAGA+TGG 0.523176 4_4:-75860436 None:intergenic
CAGGCTGGAGTTTAAATTGG+CGG 0.523456 4_4:+75864171 Msa0703820:three_prime_UTR
TGTGAGGAAAGCTTCCGTCT+TGG 0.523900 4_4:-75861729 None:intergenic
CGCAACTGCAACAAAAGTCC+GGG 0.523933 4_4:+75862652 Msa0703820:three_prime_UTR
TAGAAGGGCTTAGAGGATAT+GGG 0.526409 4_4:+75862071 Msa0703820:CDS
GTAGATCAGCCCCGAGAAGA+AGG 0.527618 4_4:+75864282 Msa0703820:three_prime_UTR
CTTGTGAAAATCTATCTCCA+TGG 0.528028 4_4:+75861798 Msa0703820:CDS
CCTTGAAAAGAGAGTTGGTA+TGG 0.528846 4_4:+75862294 Msa0703820:CDS
AACCAAAGAATCTATCAGGC+TGG 0.531250 4_4:+75864156 Msa0703820:three_prime_UTR
AGATATCAAAATGGAGGTCA+AGG 0.533332 4_4:+75862355 Msa0703820:CDS
AATTTCAGCAATTAGGGCAA+AGG 0.537477 4_4:+75861973 Msa0703820:CDS
AAAAGTCTGTTGGGATTCGA+GGG 0.537749 4_4:-75860835 None:intergenic
TATGCCAAGAGATGCCTACC+AGG 0.548221 4_4:+75862034 Msa0703820:CDS
AAACATGAAATCACTTCAAG+TGG 0.548709 4_4:+75860577 Msa0703820:exon
AGATGCTGCATCCTCACTTG+CGG 0.555801 4_4:-75861888 None:intergenic
AATGCCAATTCCAATATGAC+AGG 0.558807 4_4:-75862416 None:intergenic
TTGTGAAAATCTATCTCCAT+GGG 0.559597 4_4:+75861799 Msa0703820:CDS
TAAAAGGCTGACCTTTAGGT+AGG 0.562453 4_4:-75862823 None:intergenic
GAGAGCAGCAATGCAAGAGA+AGG 0.563979 4_4:+75863962 Msa0703820:three_prime_UTR
GGAATTGGCATTAATGCTGA+AGG 0.568875 4_4:+75862427 Msa0703820:three_prime_UTR
GCAGTAATTCAGCTAAGACT+TGG 0.571771 4_4:-75862752 None:intergenic
ATGACTGAAGAACTAGAAGA+TGG 0.572238 4_4:+75861695 Msa0703820:CDS
GATCTGTTGCAACTTCAGCT+AGG 0.573474 4_4:-75862707 None:intergenic
TAGAATTGTAGTTAAGATGG+TGG 0.574829 4_4:-75860433 None:intergenic
CAGCATCTAATCAAGAAAGC+TGG 0.575400 4_4:+75861903 Msa0703820:CDS
ATGCCTACCAGGAATGTATG+AGG 0.578553 4_4:+75862045 Msa0703820:CDS
GTTCTGCAGTTCTACCATGT+TGG 0.579053 4_4:-75864084 None:intergenic
GAGTTGATTCCACTTTGTGC+CGG 0.581941 4_4:-75863987 None:intergenic
CACGGAGAGCCTTCTTCTCG+GGG 0.584375 4_4:-75864291 None:intergenic
TCAAATGTTTCACATCCACC+TGG 0.585855 4_4:-75861584 None:intergenic
AGCCATTGACACCTACCTAA+AGG 0.587167 4_4:+75862812 Msa0703820:three_prime_UTR
CGTTCTTGGTCAGTTAGGGA+AGG 0.590168 4_4:-75862944 None:intergenic
TACCTTGTGCACATTATAGG+TGG 0.591674 4_4:-75860876 None:intergenic
CATGGGACAGCATCTTGTTG+AGG 0.601532 4_4:-75862205 None:intergenic
AGAGAGCACTAGACATAGCA+AGG 0.603854 4_4:+75862141 Msa0703820:CDS
CGAGTGTGGTTTGGTGATGT+GGG 0.605082 4_4:-75864257 None:intergenic
GGGCTTAGAGGATATGGGCA+TGG 0.605115 4_4:+75862076 Msa0703820:CDS
GAATGTATGAGGAGTTAGAA+GGG 0.605950 4_4:+75862056 Msa0703820:CDS
CGAGAATGTAAAGTCCAACA+TGG 0.615865 4_4:+75864070 Msa0703820:three_prime_UTR
CATAAAATGATCAATGCGGA+GGG 0.617020 4_4:-75861945 None:intergenic
GGGACAGCATCTTGTTGAGG+AGG 0.618679 4_4:-75862202 None:intergenic
GAGAAGGAACCGGCACAAAG+TGG 0.620698 4_4:+75863978 Msa0703820:three_prime_UTR
ATTCCTAGATATCAAAATGG+AGG 0.624406 4_4:+75862349 Msa0703820:CDS
AAAATGATCAATGCGGAGGG+TGG 0.627548 4_4:-75861942 None:intergenic
CAAAATGCGGCTACATAGGA+CGG 0.632260 4_4:+75861504 Msa0703820:CDS
TGGCAATCTCATTTCCAAGA+CGG 0.634001 4_4:+75861715 Msa0703820:CDS
AGAATTGTAGTTAAGATGGT+GGG 0.638014 4_4:-75860432 None:intergenic
ATGCGGCTACATAGGACGGT+CGG 0.639956 4_4:+75861508 Msa0703820:CDS
GCATAAAATGATCAATGCGG+AGG 0.642568 4_4:-75861946 None:intergenic
ACAGGAGGTGAAACTAGAGA+TGG 0.647018 4_4:+75864224 Msa0703820:three_prime_UTR
ACTCCTCATACATTCCTGGT+AGG 0.653906 4_4:-75862048 None:intergenic
AAGAAAGCACGACAAATGTG+AGG 0.659530 4_4:-75861745 None:intergenic
AAGCTTGAAAACTTTCCAGG+TGG 0.660259 4_4:+75861569 Msa0703820:CDS
CATCTAATCAAGAAAGCTGG+TGG 0.660897 4_4:+75861906 Msa0703820:CDS
AACCACCTATAATGTGCACA+AGG 0.667560 4_4:+75860874 Msa0703820:CDS
CCTCCCTATAGACTTCAACT+CGG 0.675286 4_4:+75861631 Msa0703820:CDS
GGGCTGATCTACGACGAGTG+TGG 0.675575 4_4:-75864271 None:intergenic
TAATGCTGAGTACTGTTCTG+TGG 0.676472 4_4:-75860462 None:intergenic
CCGAGTTGAAGTCTATAGGG+AGG 0.687521 4_4:-75861631 None:intergenic
TCTGCAACAAGAACTTACAT+GGG 0.692050 4_4:-75862222 None:intergenic
AAAGTCTGTTGGGATTCGAG+GGG 0.692715 4_4:-75860834 None:intergenic
TTCCATGTAAAACCAACAGG+AGG 0.698959 4_4:+75864209 Msa0703820:three_prime_UTR
TACCCCTTGATATCAAAATG+CGG 0.703247 4_4:+75861491 Msa0703820:CDS
TCCGCATAAAATGATCAATG+CGG 0.719094 4_4:-75861949 None:intergenic
TATGGTGTTAGAAGATGCTG+AGG 0.726855 4_4:+75862312 Msa0703820:CDS
CATACTCGATCATTTCACGA+TGG 0.744904 4_4:+75862780 Msa0703820:three_prime_UTR
AAGACATAGATGTAGTACAA+CGG 0.746121 4_4:+75862592 Msa0703820:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAATTATCTTATTAAAAA+TGG - chr4_4:75863739-75863758 None:intergenic 10.0%
!! TTTAGTTGCATAAAATATAA+AGG - chr4_4:75860740-75860759 None:intergenic 15.0%
!!! AACTTAATCTTTTTATATGT+TGG + chr4_4:75861450-75861469 Msa0703820:intron 15.0%
!!! TTTATATTTTTCCATTTGTA+AGG - chr4_4:75863435-75863454 None:intergenic 15.0%
!!! ATATAAAGGATTCTATTTCT+GGG - chr4_4:75860726-75860745 None:intergenic 20.0%
!! AATATAAAGGATTCTATTTC+TGG - chr4_4:75860727-75860746 None:intergenic 20.0%
!!! AATGATTTTCTGCATTTATA+AGG - chr4_4:75860923-75860942 None:intergenic 20.0%
!!! TGGTTTTCAAAAAAATTCTA+AGG - chr4_4:75861360-75861379 None:intergenic 20.0%
!!! ACAATTCTAAAATTCTGTTA+TGG + chr4_4:75861608-75861627 Msa0703820:CDS 20.0%
!! TTAATTCCTAGATATCAAAA+TGG + chr4_4:75862346-75862365 Msa0703820:CDS 20.0%
!! TGCGAAAAATAAAAAATAAC+CGG + chr4_4:75862463-75862482 Msa0703820:three_prime_UTR 20.0%
!! AACATTGTAATTGAAAGATT+TGG - chr4_4:75862732-75862751 None:intergenic 20.0%
!! ACAAGAAAATAGGAATTAAA+AGG - chr4_4:75862842-75862861 None:intergenic 20.0%
!!! GATTTCTTTTTCAAAGTTAA+AGG - chr4_4:75863308-75863327 None:intergenic 20.0%
!!! TTTTATTTCTAAATCACAGT+AGG - chr4_4:75863593-75863612 None:intergenic 20.0%
!! TATCTTATTAAAAATGGAGT+TGG - chr4_4:75863733-75863752 None:intergenic 20.0%
!!! GATGAAATAATTTTAAGACA+CGG - chr4_4:75864312-75864331 None:intergenic 20.0%
!! TCTGGTTTCATATATCATTT+AGG + chr4_4:75860499-75860518 Msa0703820:five_prime_UTR 25.0%
! GCATAAAAAATGAACATTCA+AGG + chr4_4:75860696-75860715 Msa0703820:CDS 25.0%
!!! TATTTTCTCCGTTTGTTTTT+TGG + chr4_4:75860788-75860807 Msa0703820:intron 25.0%
!!! CCATGTTTTTATTCTAATTC+TGG - chr4_4:75861380-75861399 None:intergenic 25.0%
! CCAGAATTAGAATAAAAACA+TGG + chr4_4:75861377-75861396 Msa0703820:intron 25.0%
!!! TTTTTTATTTTTCGCAGAGT+TGG - chr4_4:75862459-75862478 None:intergenic 25.0%
! GAAAAATAAAAAATAACCGG+AGG + chr4_4:75862466-75862485 Msa0703820:three_prime_UTR 25.0%
! AAGATAGAAATTAGTGTCTT+TGG - chr4_4:75862505-75862524 None:intergenic 25.0%
!! ATTCAATATTAGTAGGCTTT+TGG + chr4_4:75862677-75862696 Msa0703820:three_prime_UTR 25.0%
! TGAATTGAACACAAGAAAAT+AGG - chr4_4:75862852-75862871 None:intergenic 25.0%
!! TATTTTCTTGTGTTCAATTC+AGG + chr4_4:75862851-75862870 Msa0703820:intron 25.0%
! GGAATTCTTAAAACTCTATT+TGG - chr4_4:75863128-75863147 None:intergenic 25.0%
!!! GTCATCATTTTTTTTCTTCA+TGG - chr4_4:75863149-75863168 None:intergenic 25.0%
! AAAGAAGAAACTTCTTTAGA+AGG + chr4_4:75863371-75863390 Msa0703820:intron 25.0%
AGAATTGTAGTTAAGATGGT+GGG - chr4_4:75860435-75860454 None:intergenic 30.0%
TAGAATTGTAGTTAAGATGG+TGG - chr4_4:75860436-75860455 None:intergenic 30.0%
GTGTAGAATTGTAGTTAAGA+TGG - chr4_4:75860439-75860458 None:intergenic 30.0%
ACAGTACTCAGCATTAATTT+AGG + chr4_4:75860469-75860488 Msa0703820:five_prime_UTR 30.0%
AAACATGAAATCACTTCAAG+TGG + chr4_4:75860577-75860596 Msa0703820:exon 30.0%
CTTGAATTGAAAAGTCTGTT+GGG - chr4_4:75860847-75860866 None:intergenic 30.0%
ACTTGAATTGAAAAGTCTGT+TGG - chr4_4:75860848-75860867 None:intergenic 30.0%
GAATTTAACTGAGCTAAACA+AGG - chr4_4:75860948-75860967 None:intergenic 30.0%
TATCAATGATTGATACTGAC+TGG + chr4_4:75861050-75861069 Msa0703820:intron 30.0%
GATTTATACTCCCTATTAAC+AGG - chr4_4:75861089-75861108 None:intergenic 30.0%
GTCACACTGTTGTAATTAAA+AGG + chr4_4:75861399-75861418 Msa0703820:intron 30.0%
TTGTGAAAATCTATCTCCAT+GGG + chr4_4:75861799-75861818 Msa0703820:CDS 30.0%
ATACATTTACCAATGATCTC+TGG - chr4_4:75862007-75862026 None:intergenic 30.0%
ATTCCTAGATATCAAAATGG+AGG + chr4_4:75862349-75862368 Msa0703820:CDS 30.0%
!! TCATTTTTCTCCTGTCATAT+TGG + chr4_4:75862406-75862425 Msa0703820:three_prime_UTR 30.0%
AAGACATAGATGTAGTACAA+CGG + chr4_4:75862592-75862611 Msa0703820:three_prime_UTR 30.0%
CCTACTAATATTGAATTTCC+CGG - chr4_4:75862673-75862692 None:intergenic 30.0%
!! TGTGTTGATGTGTTTTTCAA+AGG - chr4_4:75863217-75863236 None:intergenic 30.0%
ATTGAAGATCTGAACCAATT+TGG + chr4_4:75863237-75863256 Msa0703820:intron 30.0%
TTGAAGATCTGAACCAATTT+GGG + chr4_4:75863238-75863257 Msa0703820:intron 30.0%
! AAACTTCTTTAGAAGGAAAG+AGG + chr4_4:75863378-75863397 Msa0703820:intron 30.0%
! AACTTCTTTAGAAGGAAAGA+GGG + chr4_4:75863379-75863398 Msa0703820:intron 30.0%
GTACAAATGATCCTTACAAA+TGG + chr4_4:75863421-75863440 Msa0703820:intron 30.0%
! ACTCTATGAGACATGATTTT+GGG - chr4_4:75863499-75863518 None:intergenic 30.0%
TGTATAGAGGAAATACTTTG+CGG - chr4_4:75863548-75863567 None:intergenic 30.0%
! ACACAAACTTTTGTGTATAG+AGG - chr4_4:75863561-75863580 None:intergenic 30.0%
AATTCCACAAAACAACATTG+TGG + chr4_4:75863757-75863776 Msa0703820:intron 30.0%
AAATTTAGGCTATATGCTTC+AGG - chr4_4:75864368-75864387 None:intergenic 30.0%
GCTATTGTGACATGTTTGTT+GGG - chr4_4:75860671-75860690 None:intergenic 35.0%
AGCTATTGTGACATGTTTGT+TGG - chr4_4:75860672-75860691 None:intergenic 35.0%
!!! TCTCCGTTTGTTTTTTGGTT+TGG + chr4_4:75860793-75860812 Msa0703820:intron 35.0%
!!! CTCCGTTTGTTTTTTGGTTT+GGG + chr4_4:75860794-75860813 Msa0703820:intron 35.0%
AGCCCAAACCAAAAAACAAA+CGG - chr4_4:75860799-75860818 None:intergenic 35.0%
! AGACTTTTCAATTCAAGTCC+AGG + chr4_4:75860850-75860869 Msa0703820:CDS 35.0%
TGATACCTTGTGCACATTAT+AGG - chr4_4:75860882-75860901 None:intergenic 35.0%
! AAGAGCACAATCCTGTTAAT+AGG + chr4_4:75861075-75861094 Msa0703820:intron 35.0%
AGAGCACAATCCTGTTAATA+GGG + chr4_4:75861076-75861095 Msa0703820:intron 35.0%
GGGAAAAATTATCTCAAGAG+AGG + chr4_4:75861192-75861211 Msa0703820:intron 35.0%
!! TGTTTTGCATGCTTTTAAGG+AGG - chr4_4:75861250-75861269 None:intergenic 35.0%
!!! TGCTGTTTTGCATGCTTTTA+AGG - chr4_4:75861253-75861272 None:intergenic 35.0%
TACCCCTTGATATCAAAATG+CGG + chr4_4:75861491-75861510 Msa0703820:CDS 35.0%
!! TAGCCGCATTTTGATATCAA+GGG - chr4_4:75861497-75861516 None:intergenic 35.0%
ATATCAAAATGCGGCTACAT+AGG + chr4_4:75861500-75861519 Msa0703820:CDS 35.0%
AGCCTCTAATCTCAAATTCT+GGG + chr4_4:75861537-75861556 Msa0703820:CDS 35.0%
! TTCCCAGAATTTGAGATTAG+AGG - chr4_4:75861542-75861561 None:intergenic 35.0%
! TATCCGAGTTGAAGTCTATA+GGG - chr4_4:75861637-75861656 None:intergenic 35.0%
! TTATCCGAGTTGAAGTCTAT+AGG - chr4_4:75861638-75861657 None:intergenic 35.0%
ATGACTGAAGAACTAGAAGA+TGG + chr4_4:75861695-75861714 Msa0703820:CDS 35.0%
CTTGTGAAAATCTATCTCCA+TGG + chr4_4:75861798-75861817 Msa0703820:CDS 35.0%
! ATTTGGATATTTTCAGCCCA+TGG - chr4_4:75861818-75861837 None:intergenic 35.0%
CACAGCATCTTCTAACAATT+TGG - chr4_4:75861835-75861854 None:intergenic 35.0%
CATAAAATGATCAATGCGGA+GGG - chr4_4:75861948-75861967 None:intergenic 35.0%
!! TCCGCATTGATCATTTTATG+CGG + chr4_4:75861948-75861967 Msa0703820:CDS 35.0%
TCCGCATAAAATGATCAATG+CGG - chr4_4:75861952-75861971 None:intergenic 35.0%
TGCGGATAATTTCAGCAATT+AGG + chr4_4:75861966-75861985 Msa0703820:CDS 35.0%
GCGGATAATTTCAGCAATTA+GGG + chr4_4:75861967-75861986 Msa0703820:CDS 35.0%
AATTTCAGCAATTAGGGCAA+AGG + chr4_4:75861973-75861992 Msa0703820:CDS 35.0%
ATTTCAGCAATTAGGGCAAA+GGG + chr4_4:75861974-75861993 Msa0703820:CDS 35.0%
TTTACCAATGATCTCTGGTT+TGG - chr4_4:75862002-75862021 None:intergenic 35.0%
GAATGTATGAGGAGTTAGAA+GGG + chr4_4:75862056-75862075 Msa0703820:CDS 35.0%
GAGGAGGAATTATGCTTATT+AGG - chr4_4:75862189-75862208 None:intergenic 35.0%
TCTGCAACAAGAACTTACAT+GGG - chr4_4:75862225-75862244 None:intergenic 35.0%
ATCTGCAACAAGAACTTACA+TGG - chr4_4:75862226-75862245 None:intergenic 35.0%
!! TGACCTCCATTTTGATATCT+AGG - chr4_4:75862355-75862374 None:intergenic 35.0%
AGATATCAAAATGGAGGTCA+AGG + chr4_4:75862355-75862374 Msa0703820:CDS 35.0%
AAATGGAGGTCAAGGAAAAA+TGG + chr4_4:75862363-75862382 Msa0703820:CDS 35.0%
TTCTCCTGTCATATTGGAAT+TGG + chr4_4:75862412-75862431 Msa0703820:three_prime_UTR 35.0%
AATGCCAATTCCAATATGAC+AGG - chr4_4:75862419-75862438 None:intergenic 35.0%
CCGGGAAATTCAATATTAGT+AGG + chr4_4:75862670-75862689 Msa0703820:three_prime_UTR 35.0%
TGAAATGATCGAGTATGTTC+TGG - chr4_4:75862777-75862796 None:intergenic 35.0%
GAATTAAAAGGCTGACCTTT+AGG - chr4_4:75862830-75862849 None:intergenic 35.0%
!! GATCTTTGGAACTTAGTCTT+CGG + chr4_4:75863080-75863099 Msa0703820:intron 35.0%
TGAAGATCTGAACCAATTTG+GGG + chr4_4:75863239-75863258 Msa0703820:intron 35.0%
AACCAAACAGCAAGACTAAT+TGG + chr4_4:75863453-75863472 Msa0703820:intron 35.0%
!! TTCCAATTAGTCTTGCTGTT+TGG - chr4_4:75863458-75863477 None:intergenic 35.0%
! CTCTATGAGACATGATTTTG+GGG - chr4_4:75863498-75863517 None:intergenic 35.0%
! CACTCTATGAGACATGATTT+TGG - chr4_4:75863500-75863519 None:intergenic 35.0%
ATCATGTCTCATAGAGTGAA+AGG + chr4_4:75863502-75863521 Msa0703820:intron 35.0%
TCATGTCTCATAGAGTGAAA+GGG + chr4_4:75863503-75863522 Msa0703820:intron 35.0%
!!! AGATCCACAATGTTGTTTTG+TGG - chr4_4:75863764-75863783 None:intergenic 35.0%
ACAAAACAACATTGTGGATC+TGG + chr4_4:75863763-75863782 Msa0703820:intron 35.0%
! ACATAGCATCTAAGATAGTG+TGG + chr4_4:75863798-75863817 Msa0703820:intron 35.0%
!! TGGACATATTAGCTCTGAAA+AGG - chr4_4:75863864-75863883 None:intergenic 35.0%
CAGAGCTAATATGTCCATAA+TGG + chr4_4:75863867-75863886 Msa0703820:intron 35.0%
TGTCTATAGTTTCAGATGTC+TGG - chr4_4:75864028-75864047 None:intergenic 35.0%
GACAAACCAAAGAATCTATC+AGG + chr4_4:75864152-75864171 Msa0703820:three_prime_UTR 35.0%
TATCAGGCTGGAGTTTAAAT+TGG + chr4_4:75864168-75864187 Msa0703820:three_prime_UTR 35.0%
! TAATGCTGAGTACTGTTCTG+TGG - chr4_4:75860465-75860484 None:intergenic 40.0%
ATTAATTTAGGCAGCGTGTC+TGG + chr4_4:75860481-75860500 Msa0703820:five_prime_UTR 40.0%
! TTCTGTCAGACTGACTTTCA+GGG - chr4_4:75860616-75860635 None:intergenic 40.0%
!!! TTTTTGGTTTGGGCTTATGC+AGG + chr4_4:75860804-75860823 Msa0703820:intron 40.0%
AAAAGTCTGTTGGGATTCGA+GGG - chr4_4:75860838-75860857 None:intergenic 40.0%
AACCACCTATAATGTGCACA+AGG + chr4_4:75860874-75860893 Msa0703820:CDS 40.0%
TACCTTGTGCACATTATAGG+TGG - chr4_4:75860879-75860898 None:intergenic 40.0%
TTCCAAACAGTATCATCAGC+AGG + chr4_4:75860968-75860987 Msa0703820:intron 40.0%
TCCAAACAGTATCATCAGCA+GGG + chr4_4:75860969-75860988 Msa0703820:intron 40.0%
TATCATCAGCAGGGTATATG+AGG + chr4_4:75860978-75860997 Msa0703820:intron 40.0%
ATCATCAGCAGGGTATATGA+GGG + chr4_4:75860979-75860998 Msa0703820:intron 40.0%
CACAAACACACTAGGAAGAA+AGG - chr4_4:75861119-75861138 None:intergenic 40.0%
AACACACACACAAACACACT+AGG - chr4_4:75861127-75861146 None:intergenic 40.0%
CCTCTTTCTCTATCCAAAAG+GGG - chr4_4:75861172-75861191 None:intergenic 40.0%
CCCTCTTTCTCTATCCAAAA+GGG - chr4_4:75861173-75861192 None:intergenic 40.0%
! CCCTTTTGGATAGAGAAAGA+GGG + chr4_4:75861170-75861189 Msa0703820:intron 40.0%
! CCTTTTGGATAGAGAAAGAG+GGG + chr4_4:75861171-75861190 Msa0703820:intron 40.0%
! CTTTTGGATAGAGAAAGAGG+GGG + chr4_4:75861172-75861191 Msa0703820:intron 40.0%
TAGACAGAGAACATCAGACA+TGG + chr4_4:75861215-75861234 Msa0703820:intron 40.0%
AGAGAACATCAGACATGGAT+AGG + chr4_4:75861220-75861239 Msa0703820:intron 40.0%
GAGAACATCAGACATGGATA+GGG + chr4_4:75861221-75861240 Msa0703820:intron 40.0%
!! AGCCGCATTTTGATATCAAG+GGG - chr4_4:75861496-75861515 None:intergenic 40.0%
!! GTAGCCGCATTTTGATATCA+AGG - chr4_4:75861498-75861517 None:intergenic 40.0%
CAGCCTCTAATCTCAAATTC+TGG + chr4_4:75861536-75861555 Msa0703820:CDS 40.0%
CTCAAGCTTGAAAACTTTCC+AGG + chr4_4:75861566-75861585 Msa0703820:CDS 40.0%
AAGCTTGAAAACTTTCCAGG+TGG + chr4_4:75861569-75861588 Msa0703820:CDS 40.0%
TCAAATGTTTCACATCCACC+TGG - chr4_4:75861587-75861606 None:intergenic 40.0%
TGGCAATCTCATTTCCAAGA+CGG + chr4_4:75861715-75861734 Msa0703820:CDS 40.0%
! AAGAAAGCACGACAAATGTG+AGG - chr4_4:75861748-75861767 None:intergenic 40.0%
CATTTGTCGTGCTTTCTTCA+TGG + chr4_4:75861750-75861769 Msa0703820:CDS 40.0%
GATGCTGTGATTCTATAGCT+TGG + chr4_4:75861846-75861865 Msa0703820:CDS 40.0%
CTGTGATTCTATAGCTTGGA+AGG + chr4_4:75861850-75861869 Msa0703820:CDS 40.0%
CAGCATCTAATCAAGAAAGC+TGG + chr4_4:75861903-75861922 Msa0703820:CDS 40.0%
CATCTAATCAAGAAAGCTGG+TGG + chr4_4:75861906-75861925 Msa0703820:CDS 40.0%
GCATAAAATGATCAATGCGG+AGG - chr4_4:75861949-75861968 None:intergenic 40.0%
TCTAACTCCTCATACATTCC+TGG - chr4_4:75862055-75862074 None:intergenic 40.0%
GGAATGTATGAGGAGTTAGA+AGG + chr4_4:75862055-75862074 Msa0703820:CDS 40.0%
TTAGAAGGGCTTAGAGGATA+TGG + chr4_4:75862070-75862089 Msa0703820:CDS 40.0%
TAGAAGGGCTTAGAGGATAT+GGG + chr4_4:75862071-75862090 Msa0703820:CDS 40.0%
CTTGTTGCAGATGCTAAAGA+TGG + chr4_4:75862234-75862253 Msa0703820:CDS 40.0%
TCAGACCTTGAAAAGAGAGT+TGG + chr4_4:75862289-75862308 Msa0703820:CDS 40.0%
! CCATACCAACTCTCTTTTCA+AGG - chr4_4:75862297-75862316 None:intergenic 40.0%
!! CCTTGAAAAGAGAGTTGGTA+TGG + chr4_4:75862294-75862313 Msa0703820:CDS 40.0%
! TATGGTGTTAGAAGATGCTG+AGG + chr4_4:75862312-75862331 Msa0703820:CDS 40.0%
GGAATTGGCATTAATGCTGA+AGG + chr4_4:75862427-75862446 Msa0703820:three_prime_UTR 40.0%
!! TGGTCATTTTCATAAGCCTC+CGG - chr4_4:75862485-75862504 None:intergenic 40.0%
! TCTTGCATAGTGCAGTTTTC+GGG - chr4_4:75862576-75862595 None:intergenic 40.0%
! GTCTTGCATAGTGCAGTTTT+CGG - chr4_4:75862577-75862596 None:intergenic 40.0%
GCAGTAATTCAGCTAAGACT+TGG - chr4_4:75862755-75862774 None:intergenic 40.0%
CATACTCGATCATTTCACGA+TGG + chr4_4:75862780-75862799 Msa0703820:three_prime_UTR 40.0%
TAAAAGGCTGACCTTTAGGT+AGG - chr4_4:75862826-75862845 None:intergenic 40.0%
TCTACGTTCTTGGTCAGTTA+GGG - chr4_4:75862951-75862970 None:intergenic 40.0%
TTCTACGTTCTTGGTCAGTT+AGG - chr4_4:75862952-75862971 None:intergenic 40.0%
GACTAAGTTCCAAAGATCCA+GGG - chr4_4:75863078-75863097 None:intergenic 40.0%
AGACTAAGTTCCAAAGATCC+AGG - chr4_4:75863079-75863098 None:intergenic 40.0%
GGTATTTAAGCAGATGCTAG+TGG - chr4_4:75863196-75863215 None:intergenic 40.0%
GAAAACATCTGACCCCAAAT+TGG - chr4_4:75863254-75863273 None:intergenic 40.0%
! AGTTTTCCATCTGCATGGAA+GGG - chr4_4:75863330-75863349 None:intergenic 40.0%
! TAGTTTTCCATCTGCATGGA+AGG - chr4_4:75863331-75863350 None:intergenic 40.0%
!!! TCACTAGTTTTCCATCTGCA+TGG - chr4_4:75863335-75863354 None:intergenic 40.0%
!! ATGAGACATGATTTTGGGGA+GGG - chr4_4:75863494-75863513 None:intergenic 40.0%
!! TATGAGACATGATTTTGGGG+AGG - chr4_4:75863495-75863514 None:intergenic 40.0%
CCTAAGCTGAATCACCATTA+TGG - chr4_4:75863884-75863903 None:intergenic 40.0%
CCATAATGGTGATTCAGCTT+AGG + chr4_4:75863881-75863900 Msa0703820:intron 40.0%
! TGCGTTGTATACTTTGGTTC+AGG - chr4_4:75863915-75863934 None:intergenic 40.0%
TGAACCAAAGTATACAACGC+AGG + chr4_4:75863914-75863933 Msa0703820:intron 40.0%
AGAACCTGCGTTGTATACTT+TGG - chr4_4:75863921-75863940 None:intergenic 40.0%
AAGTGGAATCAACTCTCAAC+AGG + chr4_4:75863995-75864014 Msa0703820:three_prime_UTR 40.0%
CGAGAATGTAAAGTCCAACA+TGG + chr4_4:75864070-75864089 Msa0703820:three_prime_UTR 40.0%
AACCAAAGAATCTATCAGGC+TGG + chr4_4:75864156-75864175 Msa0703820:three_prime_UTR 40.0%
TCCTTCCATGTAAAACCAAC+AGG + chr4_4:75864206-75864225 Msa0703820:three_prime_UTR 40.0%
!!! TCCTGTTGGTTTTACATGGA+AGG - chr4_4:75864210-75864229 None:intergenic 40.0%
TTCCATGTAAAACCAACAGG+AGG + chr4_4:75864209-75864228 Msa0703820:three_prime_UTR 40.0%
GAGCTAATTGCTGCAAGCTT+GGG - chr4_4:75860551-75860570 None:intergenic 45.0%
TGAGCTAATTGCTGCAAGCT+TGG - chr4_4:75860552-75860571 None:intergenic 45.0%
! ACTTTCAGGGTCTTGTGCTT+GGG - chr4_4:75860603-75860622 None:intergenic 45.0%
! GTTCTGTCAGACTGACTTTC+AGG - chr4_4:75860617-75860636 None:intergenic 45.0%
AAAGTCTGTTGGGATTCGAG+GGG - chr4_4:75860837-75860856 None:intergenic 45.0%
GAAAAGTCTGTTGGGATTCG+AGG - chr4_4:75860839-75860858 None:intergenic 45.0%
GCACATTATAGGTGGTTTCC+TGG - chr4_4:75860871-75860890 None:intergenic 45.0%
ACCCTGCTGATGATACTGTT+TGG - chr4_4:75860973-75860992 None:intergenic 45.0%
CTTTCTCTATCCAAAAGGGG+TGG - chr4_4:75861169-75861188 None:intergenic 45.0%
! CCCCTTTTGGATAGAGAAAG+AGG + chr4_4:75861169-75861188 Msa0703820:intron 45.0%
CCCCTCTTTCTCTATCCAAA+AGG - chr4_4:75861174-75861193 None:intergenic 45.0%
CAAAATGCGGCTACATAGGA+CGG + chr4_4:75861504-75861523 Msa0703820:CDS 45.0%
CCTCCCTATAGACTTCAACT+CGG + chr4_4:75861631-75861650 Msa0703820:CDS 45.0%
TAAAAATGAGCCCGCAAGTG+AGG + chr4_4:75861877-75861896 Msa0703820:CDS 45.0%
AAGCTGGTGGTTCGATGATT+TGG + chr4_4:75861919-75861938 Msa0703820:CDS 45.0%
AAAATGATCAATGCGGAGGG+TGG - chr4_4:75861945-75861964 None:intergenic 45.0%
GGAACCAAACCAGAGATCAT+TGG + chr4_4:75861995-75862014 Msa0703820:CDS 45.0%
ATGCCTACCAGGAATGTATG+AGG + chr4_4:75862045-75862064 Msa0703820:CDS 45.0%
ACTCCTCATACATTCCTGGT+AGG - chr4_4:75862051-75862070 None:intergenic 45.0%
!! AGAGAGCACTAGACATAGCA+AGG + chr4_4:75862141-75862160 Msa0703820:CDS 45.0%
!!! GTGCAGTTTTCGGGTGAATT+AGG - chr4_4:75862567-75862586 None:intergenic 45.0%
ACGCAACTGCAACAAAAGTC+CGG + chr4_4:75862651-75862670 Msa0703820:three_prime_UTR 45.0%
GATCTGTTGCAACTTCAGCT+AGG - chr4_4:75862710-75862729 None:intergenic 45.0%
AGCCATTGACACCTACCTAA+AGG + chr4_4:75862812-75862831 Msa0703820:three_prime_UTR 45.0%
GACCTTTAGGTAGGTGTCAA+TGG - chr4_4:75862817-75862836 None:intergenic 45.0%
TTGGTCAGTTAGGGAAGGAT+GGG - chr4_4:75862942-75862961 None:intergenic 45.0%
TGCAGAGTCTTCTACGTTCT+TGG - chr4_4:75862961-75862980 None:intergenic 45.0%
ACCTGGACAACTGTTCTTAG+AGG - chr4_4:75863049-75863068 None:intergenic 45.0%
AAGATCCAGGGAACTTTACC+TGG - chr4_4:75863066-75863085 None:intergenic 45.0%
GGTAAAGTTCCCTGGATCTT+TGG + chr4_4:75863066-75863085 Msa0703820:intron 45.0%
AGAAATCCCTTCCATGCAGA+TGG + chr4_4:75863321-75863340 Msa0703820:intron 45.0%
AGCAATGCAAGAGAAGGAAC+CGG + chr4_4:75863968-75863987 Msa0703820:three_prime_UTR 45.0%
! GAGTTGATTCCACTTTGTGC+CGG - chr4_4:75863990-75864009 None:intergenic 45.0%
GTTCTGCAGTTCTACCATGT+TGG - chr4_4:75864087-75864106 None:intergenic 45.0%
CTCCAGCCTGATAGATTCTT+TGG - chr4_4:75864161-75864180 None:intergenic 45.0%
CAGGCTGGAGTTTAAATTGG+CGG + chr4_4:75864171-75864190 Msa0703820:three_prime_UTR 45.0%
!!! CACCTCCTGTTGGTTTTACA+TGG - chr4_4:75864214-75864233 None:intergenic 45.0%
! TCTCTAGTTTCACCTCCTGT+TGG - chr4_4:75864224-75864243 None:intergenic 45.0%
ACAGGAGGTGAAACTAGAGA+TGG + chr4_4:75864224-75864243 Msa0703820:three_prime_UTR 45.0%
!! TTAGATATAATATAAAATTT+AGG - chr4_4:75864382-75864401 None:intergenic 5.0%
! GACTTTCAGGGTCTTGTGCT+TGG - chr4_4:75860604-75860623 None:intergenic 50.0%
TCTATCCAAAAGGGGTGGTG+GGG - chr4_4:75861164-75861183 None:intergenic 50.0%
CTCTATCCAAAAGGGGTGGT+GGG - chr4_4:75861165-75861184 None:intergenic 50.0%
TCTCTATCCAAAAGGGGTGG+TGG - chr4_4:75861166-75861185 None:intergenic 50.0%
! CCGAGTTGAAGTCTATAGGG+AGG - chr4_4:75861634-75861653 None:intergenic 50.0%
TGTGAGGAAAGCTTCCGTCT+TGG - chr4_4:75861732-75861751 None:intergenic 50.0%
AGATGCTGCATCCTCACTTG+CGG - chr4_4:75861891-75861910 None:intergenic 50.0%
TATGCCAAGAGATGCCTACC+AGG + chr4_4:75862034-75862053 Msa0703820:CDS 50.0%
CATTCCTGGTAGGCATCTCT+TGG - chr4_4:75862041-75862060 None:intergenic 50.0%
GAGGAGTTAGAAGGGCTTAG+AGG + chr4_4:75862064-75862083 Msa0703820:CDS 50.0%
CATGGGACAGCATCTTGTTG+AGG - chr4_4:75862208-75862227 None:intergenic 50.0%
CGCAACTGCAACAAAAGTCC+GGG + chr4_4:75862652-75862671 Msa0703820:three_prime_UTR 50.0%
CTTGGTCAGTTAGGGAAGGA+TGG - chr4_4:75862943-75862962 None:intergenic 50.0%
CGTTCTTGGTCAGTTAGGGA+AGG - chr4_4:75862947-75862966 None:intergenic 50.0%
! GCCTCTAAGAACAGTTGTCC+AGG + chr4_4:75863045-75863064 Msa0703820:three_prime_UTR 50.0%
GTTGTCCAGGTAAAGTTCCC+TGG + chr4_4:75863058-75863077 Msa0703820:intron 50.0%
GAGAGCAGCAATGCAAGAGA+AGG + chr4_4:75863962-75863981 Msa0703820:three_prime_UTR 50.0%
!! CGAGTGTGGTTTGGTGATGT+GGG - chr4_4:75864260-75864279 None:intergenic 50.0%
!! ACGAGTGTGGTTTGGTGATG+TGG - chr4_4:75864261-75864280 None:intergenic 50.0%
GATCTACGACGAGTGTGGTT+TGG - chr4_4:75864269-75864288 None:intergenic 50.0%
ATGCGGCTACATAGGACGGT+CGG + chr4_4:75861508-75861527 Msa0703820:CDS 55.0%
GATGCTGCATCCTCACTTGC+GGG - chr4_4:75861890-75861909 None:intergenic 55.0%
GGGCTTAGAGGATATGGGCA+TGG + chr4_4:75862076-75862095 Msa0703820:CDS 55.0%
GGGACAGCATCTTGTTGAGG+AGG - chr4_4:75862205-75862224 None:intergenic 55.0%
GAGAAGGAACCGGCACAAAG+TGG + chr4_4:75863978-75863997 Msa0703820:three_prime_UTR 55.0%
GTAGATCAGCCCCGAGAAGA+AGG + chr4_4:75864282-75864301 Msa0703820:three_prime_UTR 55.0%
ACACGGAGAGCCTTCTTCTC+GGG - chr4_4:75864295-75864314 None:intergenic 55.0%
GACACGGAGAGCCTTCTTCT+CGG - chr4_4:75864296-75864315 None:intergenic 55.0%
!! TCCAAAAGGGGTGGTGGGGT+GGG - chr4_4:75861160-75861179 None:intergenic 60.0%
!! ATCCAAAAGGGGTGGTGGGG+TGG - chr4_4:75861161-75861180 None:intergenic 60.0%
GGGCTGATCTACGACGAGTG+TGG - chr4_4:75864274-75864293 None:intergenic 60.0%
CACGGAGAGCCTTCTTCTCG+GGG - chr4_4:75864294-75864313 None:intergenic 60.0%
!! CCAAAAGGGGTGGTGGGGTG+GGG - chr4_4:75861159-75861178 None:intergenic 65.0%
! CCCCACCCCACCACCCCTTT+TGG + chr4_4:75861156-75861175 Msa0703820:intron 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 75860420 75864412 75860420 ID=Msa0703820;Name=Msa0703820
chr4_4 mRNA 75860420 75864412 75860420 ID=Msa0703820-mRNA-1;Parent=Msa0703820;Name=Msa0703820-mRNA-1;_AED=0.05;_eAED=0.05;_QI=161|1|1|1|0.75|0.6|5|1046|369
chr4_4 exon 75860420 75860717 75860420 ID=Msa0703820-mRNA-1:exon:23870;Parent=Msa0703820-mRNA-1
chr4_4 exon 75860826 75860895 75860826 ID=Msa0703820-mRNA-1:exon:23871;Parent=Msa0703820-mRNA-1
chr4_4 exon 75861491 75862833 75861491 ID=Msa0703820-mRNA-1:exon:23872;Parent=Msa0703820-mRNA-1
chr4_4 exon 75862938 75863066 75862938 ID=Msa0703820-mRNA-1:exon:23873;Parent=Msa0703820-mRNA-1
chr4_4 exon 75863936 75864412 75863936 ID=Msa0703820-mRNA-1:exon:23874;Parent=Msa0703820-mRNA-1
chr4_4 five_prime_UTR 75860420 75860580 75860420 ID=Msa0703820-mRNA-1:five_prime_utr;Parent=Msa0703820-mRNA-1
chr4_4 CDS 75860581 75860717 75860581 ID=Msa0703820-mRNA-1:cds;Parent=Msa0703820-mRNA-1
chr4_4 CDS 75860826 75860895 75860826 ID=Msa0703820-mRNA-1:cds;Parent=Msa0703820-mRNA-1
chr4_4 CDS 75861491 75862393 75861491 ID=Msa0703820-mRNA-1:cds;Parent=Msa0703820-mRNA-1
chr4_4 three_prime_UTR 75862394 75862833 75862394 ID=Msa0703820-mRNA-1:three_prime_utr;Parent=Msa0703820-mRNA-1
chr4_4 three_prime_UTR 75862938 75863066 75862938 ID=Msa0703820-mRNA-1:three_prime_utr;Parent=Msa0703820-mRNA-1
chr4_4 three_prime_UTR 75863936 75864412 75863936 ID=Msa0703820-mRNA-1:three_prime_utr;Parent=Msa0703820-mRNA-1
Gene Sequence

>Msa0703820

ATGAAATCACTTCAAGTGGCCCAAGCACAAGACCCTGAAAGTCAGTCTGACAGAACTGAACAAGTTTCAGATAAAAGCATAGTTATTCCCAACAAACATGTCACAATAGCTGATAGCATAAAAAATGAACATTCAAGGTTCGTTGCCCCTCGAATCCCAACAGACTTTTCAATTCAAGTCCAGGAAACCACCTATAATGTGCACAAGTACCCCTTGATATCAAAATGCGGCTACATAGGACGGTCGGAAGTACAGCCTCTAATCTCAAATTCTGGGAATGTGCTCAAGCTTGAAAACTTTCCAGGTGGATGTGAAACATTTGAAACAATTCTAAAATTCTGTTATGGCCTCCCTATAGACTTCAACTCGGATAATGTAGCTGCACTAAGATGTGCATCAGAATTTCTAGAGATGACTGAAGAACTAGAAGATGGCAATCTCATTTCCAAGACGGAAGCTTTCCTCACATTTGTCGTGCTTTCTTCATGGAAAGACACTGTTACTGTTCTGAAATCTTGTGAAAATCTATCTCCATGGGCTGAAAATATCCAAATTGTTAGAAGATGCTGTGATTCTATAGCTTGGAAGGCTACTAAAAATGAGCCCGCAAGTGAGGATGCAGCATCTAATCAAGAAAGCTGGTGGTTCGATGATTTGGCCACCCTCCGCATTGATCATTTTATGCGGATAATTTCAGCAATTAGGGCAAAGGGAACCAAACCAGAGATCATTGGTAAATGTATCATGCAATATGCCAAGAGATGCCTACCAGGAATGTATGAGGAGTTAGAAGGGCTTAGAGGATATGGGCATGGAAAAAGTAATCTACAGTTCACTATATTTAGCATGAAGAAAGAAGAGAGCACTAGACATAGCAAGGAGCAAACGACTATTATTGAAAGCCTAATAAGCATAATTCCTCCTCAACAAGATGCTGTCCCATGTAAGTTCTTGTTGCAGATGCTAAAGATGGCAATGATGTATTCTGTATCATCAGCTCTCATTTCAGACCTTGAAAAGAGAGTTGGTATGGTGTTAGAAGATGCTGAGGTGAATGATCTCTTAATTCCTAGATATCAAAATGGAGGTCAAGGAAAAATGGTCAAGTGA
Protein sequence

>Msa0703820

MKSLQVAQAQDPESQSDRTEQVSDKSIVIPNKHVTIADSIKNEHSRFVAPRIPTDFSIQVQETTYNVHKYPLISKCGYIGRSEVQPLISNSGNVLKLENFPGGCETFETILKFCYGLPIDFNSDNVAALRCASEFLEMTEELEDGNLISKTEAFLTFVVLSSWKDTVTVLKSCENLSPWAENIQIVRRCCDSIAWKATKNEPASEDAASNQESWWFDDLATLRIDHFMRIISAIRAKGTKPEIIGKCIMQYAKRCLPGMYEELEGLRGYGHGKSNLQFTIFSMKKEESTRHSKEQTTIIESLISIIPPQQDAVPCKFLLQMLKMAMMYSVSSALISDLEKRVGMVLEDAEVNDLLIPRYQNGGQGKMVK*