AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1192760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1192760 MtrunA17_Chr8g0371771 99.026 308 3 0 1 308 1 308 0.0 622
Msa1192760 MtrunA17_Chr5g0405611 62.461 317 101 5 1 304 66 377 8.82e-141 402
Msa1192760 MtrunA17_Chr4g0037331 68.644 236 70 2 11 243 5 239 4.77e-116 338
Msa1192760 MtrunA17_Chr1g0156711 62.249 249 84 5 14 252 6 254 2.14e-100 297
Msa1192760 MtrunA17_Chr3g0142781 57.795 263 99 6 8 258 3 265 6.97e-97 288
Msa1192760 MtrunA17_Chr8g0352471 55.682 264 104 7 10 263 4 264 5.42e-93 278
Msa1192760 MtrunA17_Chr4g0037351 52.830 265 109 6 13 262 3 266 5.74e-88 266
Msa1192760 MtrunA17_Chr5g0405581 55.598 259 98 8 10 252 3 260 1.40e-87 264
Msa1192760 MtrunA17_Chr8g0371781 57.917 240 85 6 28 252 25 263 4.19e-87 263
Msa1192760 MtrunA17_Chr6g0482741 54.357 241 98 6 13 244 3 240 1.49e-86 259
Msa1192760 MtrunA17_Chr7g0265011 48.800 250 119 4 5 246 41 289 1.38e-83 253
Msa1192760 MtrunA17_Chr8g0351851 56.466 232 92 5 19 243 8 237 4.14e-83 250
Msa1192760 MtrunA17_Chr1g0154591 57.265 234 88 6 23 247 5 235 9.72e-82 247
Msa1192760 MtrunA17_Chr8g0357311 52.016 248 108 6 15 253 11 256 3.47e-81 246
Msa1192760 MtrunA17_Chr5g0428801 52.964 253 99 8 8 247 8 253 6.46e-81 248
Msa1192760 MtrunA17_Chr3g0141341 56.118 237 92 7 20 247 1 234 7.28e-81 244
Msa1192760 MtrunA17_Chr8g0392961 54.812 239 97 5 14 244 10 245 3.88e-80 243
Msa1192760 MtrunA17_Chr1g0180121 50.847 236 107 4 17 244 8 242 7.02e-79 240
Msa1192760 MtrunA17_Chr1g0180161 48.729 236 112 4 19 246 10 244 1.83e-76 234
Msa1192760 MtrunA17_Chr1g0180231 50.420 238 109 4 17 246 8 244 4.37e-76 233
Msa1192760 MtrunA17_Chr1g0179871 50.420 238 109 4 17 246 8 244 4.37e-76 233
Msa1192760 MtrunA17_Chr5g0424221 46.442 267 133 6 15 276 13 274 4.77e-76 234
Msa1192760 MtrunA17_Chr2g0312181 48.536 239 112 4 17 244 5 243 8.58e-76 232
Msa1192760 MtrunA17_Chr1g0180221 49.383 243 114 4 12 246 43 284 8.65e-76 233
Msa1192760 MtrunA17_Chr1g0179891 50.427 234 107 4 21 246 7 239 1.27e-75 231
Msa1192760 MtrunA17_Chr1g0179851 48.760 242 113 5 15 246 1 241 4.11e-75 230
Msa1192760 MtrunA17_Chr2g0311521 46.538 260 117 7 27 274 20 269 3.95e-67 226
Msa1192760 MtrunA17_Chr3g0112091 46.862 239 116 7 15 244 1 237 1.32e-66 208
Msa1192760 MtrunA17_Chr2g0311551 48.052 231 109 6 33 253 25 254 3.28e-64 218
Msa1192760 MtrunA17_Chr2g0311561 45.532 235 118 6 21 246 1 234 2.37e-63 202
Msa1192760 MtrunA17_Chr7g0239131 43.697 238 124 5 15 244 7 242 6.04e-62 196
Msa1192760 MtrunA17_Chr6g0452331 44.309 246 120 9 15 245 10 253 2.95e-58 188
Msa1192760 MtrunA17_Chr4g0031221 40.664 241 125 8 15 243 17 251 3.94e-54 177
Msa1192760 MtrunA17_Chr5g0397821 42.795 229 107 9 29 251 22 232 4.39e-50 166
Msa1192760 MtrunA17_Chr5g0397831 41.350 237 120 8 16 244 11 236 7.08e-50 165
Msa1192760 MtrunA17_Chr8g0386341 41.880 234 115 11 14 244 33 248 1.59e-48 162
Msa1192760 MtrunA17_Chr2g0311531 41.176 221 108 8 28 246 24 224 2.35e-45 154
Msa1192760 MtrunA17_Chr5g0406771 48.571 175 77 6 19 182 3 175 1.45e-44 149
Msa1192760 MtrunA17_Chr2g0311501 40.642 187 101 6 69 247 1 185 1.40e-40 140
Msa1192760 MtrunA17_Chr2g0309611 32.653 245 142 8 14 242 12 249 1.10e-35 129
Msa1192760 MtrunA17_Chr1g0179911 33.884 242 141 7 13 245 8 239 5.91e-35 127
Msa1192760 MtrunA17_Chr8g0386351 42.073 164 83 8 6 167 39 192 5.92e-30 113
Msa1192760 MtrunA17_Chr1g0180181 54.762 84 34 1 167 246 18 101 5.36e-23 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1192760 AT4G38660.1 52.286 350 115 7 1 305 2 344 1.61e-113 332
Msa1192760 AT4G38660.2 55.000 320 94 6 31 305 8 322 1.15e-111 327
Msa1192760 AT4G24180.1 57.191 299 111 8 1 284 1 297 4.78e-109 320
Msa1192760 AT4G24180.2 57.576 297 108 9 1 284 1 292 2.17e-108 318
Msa1192760 AT4G24180.6 62.403 258 83 6 1 245 1 257 7.47e-107 312
Msa1192760 AT4G24180.5 62.891 256 80 7 1 245 1 252 4.96e-106 310
Msa1192760 AT4G24180.3 60.166 241 83 5 1 228 1 241 2.30e-96 288
Msa1192760 AT4G24180.4 60.669 239 80 6 1 228 1 236 1.51e-95 285
Msa1192760 AT1G20030.2 60.417 240 83 4 28 255 17 256 7.74e-93 278
Msa1192760 AT1G20030.1 61.441 236 79 4 32 255 4 239 9.17e-93 278
Msa1192760 AT1G75800.1 55.859 256 101 4 15 258 7 262 4.51e-92 277
Msa1192760 AT1G75030.1 55.020 249 98 7 8 247 3 246 1.52e-85 257
Msa1192760 AT1G75050.1 55.556 243 96 7 14 247 7 246 1.20e-84 255
Msa1192760 AT4G36010.1 57.083 240 87 6 28 252 20 258 1.40e-83 254
Msa1192760 AT4G36010.2 57.083 240 87 6 28 252 20 258 1.40e-83 254
Msa1192760 AT1G19320.1 53.755 253 100 7 1 243 1 246 1.81e-83 252
Msa1192760 AT2G17860.1 55.510 245 94 6 17 247 9 252 3.04e-83 251
Msa1192760 AT1G75040.1 54.852 237 97 6 14 244 7 239 7.66e-81 245
Msa1192760 AT4G38670.3 50.758 264 116 7 12 262 3 265 1.48e-80 246
Msa1192760 AT4G38670.1 50.758 264 116 7 12 262 3 265 1.48e-80 246
Msa1192760 AT1G73620.1 55.357 224 89 5 29 244 40 260 2.06e-78 239
Msa1192760 AT1G18250.1 54.464 224 91 5 29 244 19 239 3.90e-78 238
Msa1192760 AT1G77700.3 51.883 239 107 5 10 245 40 273 4.89e-78 241
Msa1192760 AT4G38670.2 51.626 246 108 5 28 262 4 249 5.89e-78 238
Msa1192760 AT1G18250.2 54.018 224 92 5 29 244 20 240 8.25e-78 238
Msa1192760 AT1G77700.2 49.801 251 118 5 10 257 40 285 1.04e-77 239
Msa1192760 AT5G24620.1 48.507 268 118 7 15 264 6 271 1.96e-76 240
Msa1192760 AT5G24620.4 48.507 268 118 7 15 264 61 326 3.35e-76 241
Msa1192760 AT5G24620.3 48.507 268 118 7 15 264 82 347 4.50e-76 241
Msa1192760 AT1G77700.1 54.091 220 93 5 29 245 87 301 9.20e-76 236
Msa1192760 AT5G38280.1 42.756 283 145 5 15 285 9 286 5.02e-74 240
Msa1192760 AT5G38280.2 42.756 283 145 5 15 285 9 286 5.85e-74 240
Msa1192760 AT4G36000.2 59.606 203 58 6 104 288 1 197 1.25e-67 210
Msa1192760 AT4G36000.1 59.606 203 58 6 104 288 1 197 1.25e-67 210
Msa1192760 AT5G40020.1 44.856 243 123 5 13 246 10 250 5.75e-65 205
Msa1192760 AT5G02140.1 41.892 296 150 8 9 289 3 291 7.92e-64 203
Msa1192760 AT5G02140.2 45.528 246 117 7 9 244 3 241 1.11e-62 199
Msa1192760 AT4G18253.1 42.500 240 124 5 15 246 7 240 1.33e-58 188
Msa1192760 AT1G70250.1 43.860 228 113 5 32 247 146 370 5.73e-55 191
Msa1192760 AT2G28790.1 41.841 239 125 7 17 243 12 248 3.38e-53 174
Msa1192760 AT2G28790.2 41.841 239 125 7 17 243 37 273 3.64e-53 175
Msa1192760 AT4G18250.1 41.667 240 131 4 17 249 40 277 2.31e-51 180
Msa1192760 AT4G11650.1 43.697 238 107 11 15 246 9 225 5.32e-50 166
Msa1192760 AT5G24620.2 43.275 171 81 4 134 289 15 184 1.88e-36 132
Msa1192760 AT2G24810.1 58.974 78 28 2 169 243 116 192 9.52e-24 96.3

Find 108 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAATCTTGGATAGCAAATT+TGG 0.164837 8_1:-38693390 Msa1192760:CDS
TGTACTACCTATACTATAAT+TGG 0.191819 8_1:-38695308 Msa1192760:five_prime_UTR
TTTCAGTTTGAAATCTTTAA+TGG 0.204453 8_1:-38693456 Msa1192760:intron
AGTATAGGTAGTACAAAATT+TGG 0.210723 8_1:+38695316 None:intergenic
TACGCATAGCTATAAGATTT+TGG 0.212867 8_1:+38694439 None:intergenic
AGAAGAAGGAGTAGGGGATT+AGG 0.223497 8_1:+38695407 None:intergenic
GGTTGCAAATTCGACGATTC+TGG 0.256766 8_1:-38694838 Msa1192760:CDS
GAAGAAGGAGTAGGGGATTA+GGG 0.269919 8_1:+38695408 None:intergenic
CCGGTTGGTCAGGTAGATTC+TGG 0.283059 8_1:-38694870 Msa1192760:CDS
TCAACCGGAGACTGTGGTTC+CGG 0.303738 8_1:-38694802 Msa1192760:CDS
ACAATATGGCCAGGAATTCT+AGG 0.311650 8_1:-38694961 Msa1192760:CDS
TTGTTCGCCTTCGGTTTATT+CGG 0.321676 8_1:-38694482 Msa1192760:CDS
ATCCTTGTCCTATGTCATAA+AGG 0.326258 8_1:+38693218 None:intergenic
CAACCGGAGACTGTGGTTCC+GGG 0.352374 8_1:-38694801 Msa1192760:CDS
GGCCACGGAACCTGCTCAAC+CGG 0.356065 8_1:-38694817 Msa1192760:CDS
GTTGAAACTAGTGGCGGTTC+AGG 0.356585 8_1:-38694655 Msa1192760:CDS
ACAAATGTGATCACACAATA+TGG 0.373841 8_1:-38694975 Msa1192760:CDS
GTAGAGGTGGAACTCTCTTT+AGG 0.376420 8_1:+38695362 None:intergenic
ATAGACATGGAAGTTGAAAA+TGG 0.377749 8_1:+38693338 None:intergenic
AACTCTCTTTAGGAGTGAAT+TGG 0.379146 8_1:+38695372 None:intergenic
AAACCGGTTGTTCCGAGGTC+TGG 0.386392 8_1:+38694934 None:intergenic
TATTGTTGTAGTGGCGCGTT+TGG 0.396855 8_1:-38694517 Msa1192760:CDS
AATAGCGCGTGTGGTGCCTT+TGG 0.406914 8_1:-38694550 Msa1192760:CDS
GTGGATTAGAAGAAGGAGTA+GGG 0.417664 8_1:+38695400 None:intergenic
CTGCAGTAGCTACTAAATCT+TGG 0.418679 8_1:-38693403 Msa1192760:CDS
TGTAGACGGTTACAATCTTC+CGG 0.423644 8_1:-38694683 Msa1192760:CDS
GAAATTAACTGCAACGGAAA+CGG 0.426268 8_1:-38694778 Msa1192760:CDS
CAAGCACCAGCCGGTTGGTC+AGG 0.426443 8_1:-38694880 Msa1192760:CDS
GTGAATTCTGCTAATGTTGC+CGG 0.441903 8_1:+38694745 None:intergenic
TATACATACCTTTGAAAGCT+AGG 0.444402 8_1:+38695211 None:intergenic
CGGAAACGGCGCAACACCAC+CGG 0.452277 8_1:-38694764 Msa1192760:CDS
ATTCCAACCAATTATAGTAT+AGG 0.453268 8_1:+38695301 None:intergenic
TTCCCCGGAACCACAGTCTC+CGG 0.457171 8_1:+38694798 None:intergenic
ATAGCGCGTGTGGTGCCTTT+GGG 0.460528 8_1:-38694549 Msa1192760:CDS
GCCATCGAGTTGTTCGCCTT+CGG 0.461378 8_1:-38694491 Msa1192760:CDS
TCCTTGTCCTATGTCATAAA+GGG 0.463219 8_1:+38693219 None:intergenic
AGACTAACATCGTAGTAATC+TGG 0.463483 8_1:+38694706 None:intergenic
TCATGTCAGCCCACTGGATG+TGG 0.463727 8_1:-38694631 Msa1192760:CDS
AAACCAGACCTCGGAACAAC+CGG 0.464546 8_1:-38694937 Msa1192760:CDS
TAGGGGATTAGGGAGAAAAT+TGG 0.464860 8_1:+38695418 None:intergenic
TCCGTTGCAGTTAATTTCCC+CGG 0.478576 8_1:+38694783 None:intergenic
GGAAGAGGTGGATTAGAAGA+AGG 0.482430 8_1:+38695393 None:intergenic
TCTCATACCCTTTATGACAT+AGG 0.484360 8_1:-38693226 Msa1192760:three_prime_UTR
TGGATTAGAAGAAGGAGTAG+GGG 0.484872 8_1:+38695401 None:intergenic
ATTTACCTTGGAGAAGAAGA+AGG 0.485184 8_1:+38694355 None:intergenic
CTTTGAAAGCTAGGATAAGT+AGG 0.489482 8_1:+38695220 None:intergenic
CGGTTACAATCTTCCGGTGA+TGG 0.491856 8_1:-38694677 Msa1192760:CDS
CTGACCAACCGGCTGGTGCT+TGG 0.493351 8_1:+38694881 None:intergenic
CGGTTGGTCAGGTAGATTCT+GGG 0.493549 8_1:-38694869 Msa1192760:CDS
CTACTTATCCTAGCTTTCAA+AGG 0.495706 8_1:-38695219 Msa1192760:intron
GGTGGATTAGAAGAAGGAGT+AGG 0.495979 8_1:+38695399 None:intergenic
GCTTGTTCTACTGATGATCC+TGG 0.496977 8_1:+38693425 None:intergenic
ACATGGAAGTTGAAAATGGT+TGG 0.500978 8_1:+38693342 None:intergenic
GGTAGATTCTGGGCAAGAAC+CGG 0.501018 8_1:-38694859 Msa1192760:CDS
ACCACAGTCTCCGGTTGAGC+AGG 0.501097 8_1:+38694807 None:intergenic
TAGGAGTGAATTGGAAGAGG+TGG 0.506212 8_1:+38695381 None:intergenic
CCAACCGGCTGGTGCTTGGA+AGG 0.506602 8_1:+38694885 None:intergenic
TAAGTAGGAAAATGAAGAAA+CGG 0.509403 8_1:+38695235 None:intergenic
TATTCTTGTGGAAGCTCCTG+TGG 0.511782 8_1:+38693367 None:intergenic
TTTAATTTGTACTGTAGAGG+TGG 0.511893 8_1:+38695349 None:intergenic
CTTTAGGAGTGAATTGGAAG+AGG 0.512519 8_1:+38695378 None:intergenic
GATCAAACAACATAAGTAGT+TGG 0.514391 8_1:-38693198 Msa1192760:three_prime_UTR
GATGTGGAGAGGATCTTAAC+CGG 0.515133 8_1:-38694615 Msa1192760:CDS
AGAATCGTCGAATTTGCAAC+CGG 0.526091 8_1:+38694840 None:intergenic
CCGTCCACTCTCAGCTCCGA+TGG 0.526529 8_1:+38694586 None:intergenic
CCAGAATCTACCTGACCAAC+CGG 0.527173 8_1:+38694870 None:intergenic
TCTTTAATGGAGTCAAAGCC+AGG 0.528098 8_1:-38693443 Msa1192760:CDS
TCAGGTTCATGTCAGCCCAC+TGG 0.530267 8_1:-38694637 Msa1192760:CDS
CTACCTATACTATAATTGGT+TGG 0.532469 8_1:-38695304 Msa1192760:five_prime_UTR
TGTGATCACACAATATGGCC+AGG 0.532969 8_1:-38694970 Msa1192760:CDS
TTTGTCCTTCTTCTTCTCCA+AGG 0.536796 8_1:-38694360 Msa1192760:intron
TGGATAGCAAATTTGGCCAC+AGG 0.539560 8_1:-38693383 Msa1192760:CDS
GTGTGGTGCCTTTGGGACAC+CGG 0.551624 8_1:-38694542 Msa1192760:CDS
AAATTCGACGATTCTGGCCA+CGG 0.552629 8_1:-38694832 Msa1192760:CDS
AAATGGTTGGCTTATTCTTG+TGG 0.566106 8_1:+38693355 None:intergenic
AACATTTCCGAATAAACCGA+AGG 0.568030 8_1:+38694475 None:intergenic
TATGCAAATGAGATGTCACT+TGG 0.568469 8_1:+38693252 None:intergenic
CCATCGGAGCTGAGAGTGGA+CGG 0.581824 8_1:-38694586 Msa1192760:CDS
GTGTCCATCGGAGCTGAGAG+TGG 0.585045 8_1:-38694590 Msa1192760:CDS
CTCCGGTTGAGCAGGTTCCG+TGG 0.586582 8_1:+38694815 None:intergenic
ACACCGGAGTATTGTTGTAG+TGG 0.587807 8_1:-38694526 Msa1192760:CDS
CCACTAGTTTCAACCATCAC+CGG 0.588717 8_1:+38694664 None:intergenic
TCCGGGGAAATTAACTGCAA+CGG 0.590336 8_1:-38694784 Msa1192760:CDS
AATCTACCTGACCAACCGGC+TGG 0.593577 8_1:+38694874 None:intergenic
TACGATGTTAGTCTTGTAGA+CGG 0.595413 8_1:-38694697 Msa1192760:CDS
ACATTAGCAGAATTCACTCT+AGG 0.595724 8_1:-38694739 Msa1192760:CDS
ACCGAAGGCGAACAACTCGA+TGG 0.601385 8_1:+38694490 None:intergenic
AACCGGAGACTGTGGTTCCG+GGG 0.604183 8_1:-38694800 Msa1192760:CDS
AAGATCCTCTCCACATCCAG+TGG 0.606360 8_1:+38694621 None:intergenic
TCAGCTCCGATGGACACCTC+CGG 0.607057 8_1:+38694596 None:intergenic
ACCTGCTCAACCGGAGACTG+TGG 0.608729 8_1:-38694808 Msa1192760:CDS
ACAATACTCCGGTGTCCCAA+AGG 0.614212 8_1:+38694534 None:intergenic
TCTTAACCGGAGGTGTCCAT+CGG 0.615290 8_1:-38694602 Msa1192760:CDS
TAGTACAAAATTTGGAACAG+AGG 0.618607 8_1:+38695324 None:intergenic
CCTTCCAAGCACCAGCCGGT+TGG 0.621791 8_1:-38694885 Msa1192760:CDS
GTTCAAAACCGGTTGTTCCG+AGG 0.625163 8_1:+38694929 None:intergenic
CAAACCTTCCAAGCACCAGC+CGG 0.627518 8_1:-38694889 Msa1192760:CDS
CCGGTGATGGTTGAAACTAG+TGG 0.635688 8_1:-38694664 Msa1192760:CDS
AGATCCTCTCCACATCCAGT+GGG 0.647063 8_1:+38694622 None:intergenic
GACGCGTGTAATAGCGCGTG+TGG 0.652762 8_1:-38694559 Msa1192760:CDS
TCAGCCCACTGGATGTGGAG+AGG 0.658980 8_1:-38694626 Msa1192760:CDS
ACCCTTTATGACATAGGACA+AGG 0.663858 8_1:-38693220 Msa1192760:three_prime_UTR
ATTCTAGGAAAACCAGACCT+CGG 0.680245 8_1:-38694946 Msa1192760:CDS
AATTCTGCTAATGTTGCCGG+TGG 0.701173 8_1:+38694748 None:intergenic
GCGCCACTACAACAATACTC+CGG 0.703825 8_1:+38694523 None:intergenic
GTGGAGAGGATCTTAACCGG+AGG 0.704810 8_1:-38694612 Msa1192760:CDS
TCGGAGCTGAGAGTGGACGG+TGG 0.723814 8_1:-38694583 Msa1192760:CDS
GTGATGGTTGAAACTAGTGG+CGG 0.740161 8_1:-38694661 Msa1192760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTCATATATGTATTGA+TGG + chr8_1:38693451-38693470 None:intergenic 15.0%
!!! ACTTTTCACTTTTTTTTTTT+AGG - chr8_1:38694232-38694251 Msa1192760:intron 15.0%
!!! CTTTTCACTTTTTTTTTTTA+GGG - chr8_1:38694233-38694252 Msa1192760:intron 15.0%
!!! TTTTAAGAGTAAAAAATGAA+AGG - chr8_1:38694697-38694716 Msa1192760:CDS 15.0%
!!! TGAATTTTACAAAAGAAAAT+TGG - chr8_1:38694802-38694821 Msa1192760:CDS 15.0%
!!! GAATCAGTTAAAAGTTTTAA+TGG - chr8_1:38694608-38694627 Msa1192760:CDS 20.0%
!!! CTTTTGTAAAATTCAGAATT+TGG + chr8_1:38694797-38694816 None:intergenic 20.0%
!! ACTATCAATTAAGATACAAT+AGG + chr8_1:38694988-38695007 None:intergenic 20.0%
!!! TTTCAGTTTGAAATCTTTAA+TGG - chr8_1:38695076-38695095 Msa1192760:intron 20.0%
!!! GATTTTAATTTGTACTGTAG+AGG + chr8_1:38693189-38693208 None:intergenic 25.0%
! AGTATAGGTAGTACAAAATT+TGG + chr8_1:38693219-38693238 None:intergenic 25.0%
! TGTACTACCTATACTATAAT+TGG - chr8_1:38693224-38693243 Msa1192760:three_prime_UTR 25.0%
! ATTCCAACCAATTATAGTAT+AGG + chr8_1:38693234-38693253 None:intergenic 25.0%
! TAAGTAGGAAAATGAAGAAA+CGG + chr8_1:38693300-38693319 None:intergenic 25.0%
!! ATATAACGACGATTTTTGAT+TGG + chr8_1:38693413-38693432 None:intergenic 25.0%
!!! GTGAAAAGTTTTATTTACCT+TGG + chr8_1:38694192-38694211 None:intergenic 25.0%
!! AACTTTTCACTCTTTAATCA+AGG - chr8_1:38694264-38694283 Msa1192760:intron 25.0%
!!! CCTATTTTTTGTTTTGTCAA+AGG + chr8_1:38694557-38694576 None:intergenic 25.0%
! CCTTTGACAAAACAAAAAAT+AGG - chr8_1:38694554-38694573 Msa1192760:CDS 25.0%
!!! TATTGATGATGAAATTGTTC+TGG - chr8_1:38694586-38694605 Msa1192760:CDS 25.0%
! ATCAATTAAGATACAATAGG+TGG + chr8_1:38694985-38695004 None:intergenic 25.0%
!! GTGATGTGAAAATCTTTTAA+TGG - chr8_1:38695008-38695027 Msa1192760:CDS 25.0%
! TAAATCTTGGATAGCAAATT+TGG - chr8_1:38695142-38695161 Msa1192760:intron 25.0%
! AGCAACAAAAAATATAGACA+TGG + chr8_1:38695210-38695229 None:intergenic 25.0%
!! TTTAATTTGTACTGTAGAGG+TGG + chr8_1:38693186-38693205 None:intergenic 30.0%
! TAGTACAAAATTTGGAACAG+AGG + chr8_1:38693211-38693230 None:intergenic 30.0%
CTACCTATACTATAATTGGT+TGG - chr8_1:38693228-38693247 Msa1192760:three_prime_UTR 30.0%
! TATACATACCTTTGAAAGCT+AGG + chr8_1:38693324-38693343 None:intergenic 30.0%
ACAAATGTGATCACACAATA+TGG - chr8_1:38693557-38693576 Msa1192760:intron 30.0%
!!! TCCTTTTTTGAGTTCAAAAC+CGG + chr8_1:38693617-38693636 None:intergenic 30.0%
! TACGCATAGCTATAAGATTT+TGG + chr8_1:38694096-38694115 None:intergenic 30.0%
TTAACCAACAACCTTTGATA+GGG + chr8_1:38694307-38694326 None:intergenic 30.0%
TTTAACCAACAACCTTTGAT+AGG + chr8_1:38694308-38694327 None:intergenic 30.0%
GTTGGTTAAACAAGTTGTTA+AGG - chr8_1:38694318-38694337 Msa1192760:intron 30.0%
! AAGTTGTTAAGGACATCTTT+TGG - chr8_1:38694329-38694348 Msa1192760:intron 30.0%
CTTACAACTATCAAAATCCA+AGG - chr8_1:38694456-38694475 Msa1192760:CDS 30.0%
GAATGTTGTGATGAAAGATT+AGG - chr8_1:38694647-38694666 Msa1192760:CDS 30.0%
AGAAATTGAGTGTATGTCAT+TGG - chr8_1:38694760-38694779 Msa1192760:CDS 30.0%
ATAGACATGGAAGTTGAAAA+TGG + chr8_1:38695197-38695216 None:intergenic 30.0%
GATCAAACAACATAAGTAGT+TGG - chr8_1:38695334-38695353 Msa1192760:five_prime_UTR 30.0%
! AACTCTCTTTAGGAGTGAAT+TGG + chr8_1:38693163-38693182 None:intergenic 35.0%
! CTTTGAAAGCTAGGATAAGT+AGG + chr8_1:38693315-38693334 None:intergenic 35.0%
! CTACTTATCCTAGCTTTCAA+AGG - chr8_1:38693313-38693332 Msa1192760:CDS 35.0%
ACAATTATTCCCATACATGC+AGG - chr8_1:38693499-38693518 Msa1192760:intron 35.0%
ATTGTTACTCCTGCATGTAT+GGG + chr8_1:38693511-38693530 None:intergenic 35.0%
AATTGTTACTCCTGCATGTA+TGG + chr8_1:38693512-38693531 None:intergenic 35.0%
!!! ACCGGTTTTGAACTCAAAAA+AGG - chr8_1:38693613-38693632 Msa1192760:intron 35.0%
GAAATTAACTGCAACGGAAA+CGG - chr8_1:38693754-38693773 Msa1192760:intron 35.0%
ACATTAGCAGAATTCACTCT+AGG - chr8_1:38693793-38693812 Msa1192760:intron 35.0%
AGACTAACATCGTAGTAATC+TGG + chr8_1:38693829-38693848 None:intergenic 35.0%
!! TACGATGTTAGTCTTGTAGA+CGG - chr8_1:38693835-38693854 Msa1192760:intron 35.0%
AACATTTCCGAATAAACCGA+AGG + chr8_1:38694060-38694079 None:intergenic 35.0%
! GCTATAAGATTTTGGACAAG+CGG + chr8_1:38694088-38694107 None:intergenic 35.0%
! TACTTTTACTTGTACTGCTG+CGG - chr8_1:38694131-38694150 Msa1192760:intron 35.0%
ATTTACCTTGGAGAAGAAGA+AGG + chr8_1:38694180-38694199 None:intergenic 35.0%
TAACCAACAACCTTTGATAG+GGG + chr8_1:38694306-38694325 None:intergenic 35.0%
! TTGTAAGATCGATTTAGTGC+AGG + chr8_1:38694443-38694462 None:intergenic 35.0%
ACAACTATCAAAATCCAAGG+TGG - chr8_1:38694459-38694478 Msa1192760:CDS 35.0%
!! GATGCAATCTTGATTTGTTG+TGG - chr8_1:38694484-38694503 Msa1192760:CDS 35.0%
ACAATAGGTGGAAAGAAGAA+AGG + chr8_1:38694973-38694992 None:intergenic 35.0%
CAACATCTTCAATTATGTCG+TGG + chr8_1:38695035-38695054 None:intergenic 35.0%
!! AAATGGTTGGCTTATTCTTG+TGG + chr8_1:38695180-38695199 None:intergenic 35.0%
ACATGGAAGTTGAAAATGGT+TGG + chr8_1:38695193-38695212 None:intergenic 35.0%
TATGCAAATGAGATGTCACT+TGG + chr8_1:38695283-38695302 None:intergenic 35.0%
TCTCATACCCTTTATGACAT+AGG - chr8_1:38695306-38695325 Msa1192760:five_prime_UTR 35.0%
TCCTTGTCCTATGTCATAAA+GGG + chr8_1:38695316-38695335 None:intergenic 35.0%
ATCCTTGTCCTATGTCATAA+AGG + chr8_1:38695317-38695336 None:intergenic 35.0%
! TGGATTAGAAGAAGGAGTAG+GGG + chr8_1:38693134-38693153 None:intergenic 40.0%
!! GTGGATTAGAAGAAGGAGTA+GGG + chr8_1:38693135-38693154 None:intergenic 40.0%
! CTTTAGGAGTGAATTGGAAG+AGG + chr8_1:38693157-38693176 None:intergenic 40.0%
ACAATATGGCCAGGAATTCT+AGG - chr8_1:38693571-38693590 Msa1192760:intron 40.0%
!!! TCTGGTTTTCCTAGAATTCC+TGG + chr8_1:38693583-38693602 None:intergenic 40.0%
! ATTCTAGGAAAACCAGACCT+CGG - chr8_1:38693586-38693605 Msa1192760:intron 40.0%
AGAATCGTCGAATTTGCAAC+CGG + chr8_1:38693695-38693714 None:intergenic 40.0%
GTGAATTCTGCTAATGTTGC+CGG + chr8_1:38693790-38693809 None:intergenic 40.0%
TGTAGACGGTTACAATCTTC+CGG - chr8_1:38693849-38693868 Msa1192760:intron 40.0%
TTGTTCGCCTTCGGTTTATT+CGG - chr8_1:38694050-38694069 Msa1192760:intron 40.0%
TTTGTCCTTCTTCTTCTCCA+AGG - chr8_1:38694172-38694191 Msa1192760:intron 40.0%
CTCAATTTCACCCCTATCAA+AGG - chr8_1:38694293-38694312 Msa1192760:intron 40.0%
TCTTTAATGGAGTCAAAGCC+AGG - chr8_1:38695089-38695108 Msa1192760:intron 40.0%
CTGCAGTAGCTACTAAATCT+TGG - chr8_1:38695129-38695148 Msa1192760:intron 40.0%
ACCCTTTATGACATAGGACA+AGG - chr8_1:38695312-38695331 Msa1192760:five_prime_UTR 40.0%
GAAGAAGGAGTAGGGGATTA+GGG + chr8_1:38693127-38693146 None:intergenic 45.0%
AGAAGAAGGAGTAGGGGATT+AGG + chr8_1:38693128-38693147 None:intergenic 45.0%
!! GGTGGATTAGAAGAAGGAGT+AGG + chr8_1:38693136-38693155 None:intergenic 45.0%
!! GGAAGAGGTGGATTAGAAGA+AGG + chr8_1:38693142-38693161 None:intergenic 45.0%
TAGGAGTGAATTGGAAGAGG+TGG + chr8_1:38693154-38693173 None:intergenic 45.0%
GTAGAGGTGGAACTCTCTTT+AGG + chr8_1:38693173-38693192 None:intergenic 45.0%
TGTGATCACACAATATGGCC+AGG - chr8_1:38693562-38693581 Msa1192760:intron 45.0%
GGTTGCAAATTCGACGATTC+TGG - chr8_1:38693694-38693713 Msa1192760:intron 45.0%
AAATTCGACGATTCTGGCCA+CGG - chr8_1:38693700-38693719 Msa1192760:intron 45.0%
TCCGGGGAAATTAACTGCAA+CGG - chr8_1:38693748-38693767 Msa1192760:intron 45.0%
TCCGTTGCAGTTAATTTCCC+CGG + chr8_1:38693752-38693771 None:intergenic 45.0%
AATTCTGCTAATGTTGCCGG+TGG + chr8_1:38693787-38693806 None:intergenic 45.0%
! CCACTAGTTTCAACCATCAC+CGG + chr8_1:38693871-38693890 None:intergenic 45.0%
GTGATGGTTGAAACTAGTGG+CGG - chr8_1:38693871-38693890 Msa1192760:intron 45.0%
GATGTGGAGAGGATCTTAAC+CGG - chr8_1:38693917-38693936 Msa1192760:intron 45.0%
!! ACACCGGAGTATTGTTGTAG+TGG - chr8_1:38694006-38694025 Msa1192760:intron 45.0%
TATTGTTGTAGTGGCGCGTT+TGG - chr8_1:38694015-38694034 Msa1192760:intron 45.0%
TCACCCCTATCAAAGGTTGT+TGG - chr8_1:38694300-38694319 Msa1192760:intron 45.0%
GCTTGTTCTACTGATGATCC+TGG + chr8_1:38695110-38695129 None:intergenic 45.0%
TGGATAGCAAATTTGGCCAC+AGG - chr8_1:38695149-38695168 Msa1192760:intron 45.0%
TATTCTTGTGGAAGCTCCTG+TGG + chr8_1:38695168-38695187 None:intergenic 45.0%
AAACCAGACCTCGGAACAAC+CGG - chr8_1:38693595-38693614 Msa1192760:intron 50.0%
GTTCAAAACCGGTTGTTCCG+AGG + chr8_1:38693606-38693625 None:intergenic 50.0%
CCAGAATCTACCTGACCAAC+CGG + chr8_1:38693665-38693684 None:intergenic 50.0%
! CGGTTGGTCAGGTAGATTCT+GGG - chr8_1:38693663-38693682 Msa1192760:intron 50.0%
GGTAGATTCTGGGCAAGAAC+CGG - chr8_1:38693673-38693692 Msa1192760:intron 50.0%
CGGTTACAATCTTCCGGTGA+TGG - chr8_1:38693855-38693874 Msa1192760:intron 50.0%
! CCGGTGATGGTTGAAACTAG+TGG - chr8_1:38693868-38693887 Msa1192760:intron 50.0%
GTTGAAACTAGTGGCGGTTC+AGG - chr8_1:38693877-38693896 Msa1192760:intron 50.0%
AGATCCTCTCCACATCCAGT+GGG + chr8_1:38693913-38693932 None:intergenic 50.0%
AAGATCCTCTCCACATCCAG+TGG + chr8_1:38693914-38693933 None:intergenic 50.0%
!! TCTTAACCGGAGGTGTCCAT+CGG - chr8_1:38693930-38693949 Msa1192760:intron 50.0%
ACAATACTCCGGTGTCCCAA+AGG + chr8_1:38694001-38694020 None:intergenic 50.0%
GCGCCACTACAACAATACTC+CGG + chr8_1:38694012-38694031 None:intergenic 50.0%
! CAAGATTGCATCGACCACCT+TGG + chr8_1:38694476-38694495 None:intergenic 50.0%
AAACCGGTTGTTCCGAGGTC+TGG + chr8_1:38693601-38693620 None:intergenic 55.0%
CAAACCTTCCAAGCACCAGC+CGG - chr8_1:38693643-38693662 Msa1192760:intron 55.0%
AATCTACCTGACCAACCGGC+TGG + chr8_1:38693661-38693680 None:intergenic 55.0%
! CCGGTTGGTCAGGTAGATTC+TGG - chr8_1:38693662-38693681 Msa1192760:intron 55.0%
TCAACCGGAGACTGTGGTTC+CGG - chr8_1:38693730-38693749 Msa1192760:intron 55.0%
TCAGGTTCATGTCAGCCCAC+TGG - chr8_1:38693895-38693914 Msa1192760:intron 55.0%
TCATGTCAGCCCACTGGATG+TGG - chr8_1:38693901-38693920 Msa1192760:intron 55.0%
GTGGAGAGGATCTTAACCGG+AGG - chr8_1:38693920-38693939 Msa1192760:intron 55.0%
AATAGCGCGTGTGGTGCCTT+TGG - chr8_1:38693982-38694001 Msa1192760:intron 55.0%
ATAGCGCGTGTGGTGCCTTT+GGG - chr8_1:38693983-38694002 Msa1192760:intron 55.0%
! GCCATCGAGTTGTTCGCCTT+CGG - chr8_1:38694041-38694060 Msa1192760:intron 55.0%
ACCGAAGGCGAACAACTCGA+TGG + chr8_1:38694045-38694064 None:intergenic 55.0%
ACCTGCTCAACCGGAGACTG+TGG - chr8_1:38693724-38693743 Msa1192760:intron 60.0%
ACCACAGTCTCCGGTTGAGC+AGG + chr8_1:38693728-38693747 None:intergenic 60.0%
CAACCGGAGACTGTGGTTCC+GGG - chr8_1:38693731-38693750 Msa1192760:intron 60.0%
AACCGGAGACTGTGGTTCCG+GGG - chr8_1:38693732-38693751 Msa1192760:intron 60.0%
TTCCCCGGAACCACAGTCTC+CGG + chr8_1:38693737-38693756 None:intergenic 60.0%
TCAGCCCACTGGATGTGGAG+AGG - chr8_1:38693906-38693925 Msa1192760:intron 60.0%
TCAGCTCCGATGGACACCTC+CGG + chr8_1:38693939-38693958 None:intergenic 60.0%
GTGTCCATCGGAGCTGAGAG+TGG - chr8_1:38693942-38693961 Msa1192760:intron 60.0%
CCATCGGAGCTGAGAGTGGA+CGG - chr8_1:38693946-38693965 Msa1192760:intron 60.0%
GACGCGTGTAATAGCGCGTG+TGG - chr8_1:38693973-38693992 Msa1192760:intron 60.0%
!! GTGTGGTGCCTTTGGGACAC+CGG - chr8_1:38693990-38694009 Msa1192760:intron 60.0%
CCAACCGGCTGGTGCTTGGA+AGG + chr8_1:38693650-38693669 None:intergenic 65.0%
CCTTCCAAGCACCAGCCGGT+TGG - chr8_1:38693647-38693666 Msa1192760:intron 65.0%
CTGACCAACCGGCTGGTGCT+TGG + chr8_1:38693654-38693673 None:intergenic 65.0%
! CAAGCACCAGCCGGTTGGTC+AGG - chr8_1:38693652-38693671 Msa1192760:intron 65.0%
GGCCACGGAACCTGCTCAAC+CGG - chr8_1:38693715-38693734 Msa1192760:intron 65.0%
CTCCGGTTGAGCAGGTTCCG+TGG + chr8_1:38693720-38693739 None:intergenic 65.0%
CGGAAACGGCGCAACACCAC+CGG - chr8_1:38693768-38693787 Msa1192760:intron 65.0%
CCGTCCACTCTCAGCTCCGA+TGG + chr8_1:38693949-38693968 None:intergenic 65.0%
TCGGAGCTGAGAGTGGACGG+TGG - chr8_1:38693949-38693968 Msa1192760:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 38693115 38695439 38693115 ID=Msa1192760;Name=Msa1192760
chr8_1 mRNA 38693115 38695439 38693115 ID=Msa1192760-mRNA-1;Parent=Msa1192760;Name=Msa1192760-mRNA-1;_AED=0.04;_eAED=0.04;_QI=147|1|1|1|1|1|3|177|308
chr8_1 exon 38693115 38693472 38693115 ID=Msa1192760-mRNA-1:exon:12667;Parent=Msa1192760-mRNA-1
chr8_1 exon 38694361 38695033 38694361 ID=Msa1192760-mRNA-1:exon:12666;Parent=Msa1192760-mRNA-1
chr8_1 exon 38695220 38695439 38695220 ID=Msa1192760-mRNA-1:exon:12665;Parent=Msa1192760-mRNA-1
chr8_1 five_prime_UTR 38695293 38695439 38695293 ID=Msa1192760-mRNA-1:five_prime_utr;Parent=Msa1192760-mRNA-1
chr8_1 CDS 38695220 38695292 38695220 ID=Msa1192760-mRNA-1:cds;Parent=Msa1192760-mRNA-1
chr8_1 CDS 38694361 38695033 38694361 ID=Msa1192760-mRNA-1:cds;Parent=Msa1192760-mRNA-1
chr8_1 CDS 38693292 38693472 38693292 ID=Msa1192760-mRNA-1:cds;Parent=Msa1192760-mRNA-1
chr8_1 three_prime_UTR 38693115 38693291 38693115 ID=Msa1192760-mRNA-1:three_prime_utr;Parent=Msa1192760-mRNA-1
Gene Sequence

>Msa1192760

ATGTCTATGTTCTCATATCACAAACATTCTTCTAACCGTTTCTTCATTTTCCTACTTATCCTAGCTTTCAAAGGAGTAACAATTTCAGCAACAACATTCACGTTCGTGAACAAATGTGATCACACAATATGGCCAGGAATTCTAGGAAAACCAGACCTCGGAACAACCGGTTTTGAACTCAAAAAAGGAAACACACAAACCTTCCAAGCACCAGCCGGTTGGTCAGGTAGATTCTGGGCAAGAACCGGTTGCAAATTCGACGATTCTGGCCACGGAACCTGCTCAACCGGAGACTGTGGTTCCGGGGAAATTAACTGCAACGGAAACGGCGCAACACCACCGGCAACATTAGCAGAATTCACTCTAGGAAATAGTTCGCCAGATTACTACGATGTTAGTCTTGTAGACGGTTACAATCTTCCGGTGATGGTTGAAACTAGTGGCGGTTCAGGTTCATGTCAGCCCACTGGATGTGGAGAGGATCTTAACCGGAGGTGTCCATCGGAGCTGAGAGTGGACGGTGGTGACGCGTGTAATAGCGCGTGTGGTGCCTTTGGGACACCGGAGTATTGTTGTAGTGGCGCGTTTGGTTCGCCATCGAGTTGTTCGCCTTCGGTTTATTCGGAAATGTTTAAATCCGCTTGTCCAAAATCTTATAGCTATGCGTATGATGATGCTACGAGTACTTTTACTTGTACTGCTGCGGATGATTATACTATCACATTTTGTCCTTCTTCTTCTCCAAGTTTGAAATCTTTAATGGAGTCAAAGCCAGGATCATCAGTAGAACAAGCTGCAGTAGCTACTAAATCTTGGATAGCAAATTTGGCCACAGGAGCTTCCACAAGAATAAGCCAACCATTTTCAACTTCCATGTCTATATTTTTTGTTGCTATCACTTTCATATTTTCATATTTAGTCAATTAG
Protein sequence

>Msa1192760

MSMFSYHKHSSNRFFIFLLILAFKGVTISATTFTFVNKCDHTIWPGILGKPDLGTTGFELKKGNTQTFQAPAGWSGRFWARTGCKFDDSGHGTCSTGDCGSGEINCNGNGATPPATLAEFTLGNSSPDYYDVSLVDGYNLPVMVETSGGSGSCQPTGCGEDLNRRCPSELRVDGGDACNSACGAFGTPEYCCSGAFGSPSSCSPSVYSEMFKSACPKSYSYAYDDATSTFTCTAADDYTITFCPSSSPSLKSLMESKPGSSVEQAAVATKSWIANLATGASTRISQPFSTSMSIFFVAITFIFSYLVN*