AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0420750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0420750 MtrunA17_Chr3g0120521 84.270 89 14 0 1 89 506 594 1.40e-46 158
Msa0420750 MtrunA17_Chr7g0261701 62.366 93 35 0 1 93 29 121 1.20e-39 133
Msa0420750 MtrunA17_Chr4g0076611 61.290 93 36 0 1 93 433 525 1.43e-36 130
Msa0420750 MtrunA17_Chr4g0057531 61.290 93 36 0 1 93 510 602 2.49e-35 127
Msa0420750 MtrunA17_Chr4g0057511 60.215 93 37 0 1 93 349 441 9.76e-35 125
Msa0420750 MtrunA17_Chr4g0057481 61.290 93 36 0 1 93 423 515 2.56e-34 124
Msa0420750 MtrunA17_Chr5g0447171 66.667 90 21 1 1 90 207 287 4.40e-34 122
Msa0420750 MtrunA17_Chr4g0057491 58.065 93 39 0 1 93 355 447 1.65e-33 121
Msa0420750 MtrunA17_Chr5g0439271 64.045 89 32 0 1 89 337 425 4.12e-33 120
Msa0420750 MtrunA17_Chr4g0057461 57.778 90 38 0 1 90 361 450 5.97e-33 120
Msa0420750 MtrunA17_Chr4g0057471 58.065 93 39 0 1 93 442 534 1.44e-32 119
Msa0420750 MtrunA17_Chr8g0344331 61.798 89 34 0 1 89 502 590 2.40e-32 118
Msa0420750 MtrunA17_Chr7g0250481 62.222 90 33 1 1 89 531 620 3.62e-32 118
Msa0420750 MtrunA17_Chr4g0053781 53.933 89 41 0 1 89 484 572 9.93e-32 116
Msa0420750 MtrunA17_Chr7g0250441 57.447 94 39 1 1 93 16 109 1.11e-31 113
Msa0420750 MtrunA17_Chr5g0401511 55.056 89 40 0 1 89 490 578 4.86e-31 114
Msa0420750 MtrunA17_Chr7g0250371 60.000 90 35 1 1 89 518 607 7.13e-31 114
Msa0420750 MtrunA17_Chr5g0423261 55.914 93 41 0 1 93 355 447 1.63e-30 113
Msa0420750 MtrunA17_Chr7g0250331 60.000 90 35 1 1 89 539 628 2.02e-30 113
Msa0420750 MtrunA17_Chr4g0020651 52.874 87 41 0 1 87 486 572 2.41e-30 112
Msa0420750 MtrunA17_Chr7g0250411 60.000 90 35 1 1 89 517 606 7.87e-30 111
Msa0420750 MtrunA17_Chr4g0037221 52.809 89 42 0 1 89 492 580 8.31e-30 111
Msa0420750 MtrunA17_Chr1g0152771 53.933 89 41 0 1 89 482 570 8.38e-30 111
Msa0420750 MtrunA17_Chr1g0204321 57.303 89 38 0 1 89 507 595 8.40e-30 111
Msa0420750 MtrunA17_Chr7g0250391 57.778 90 37 1 1 89 516 605 1.24e-29 110
Msa0420750 MtrunA17_Chr2g0300751 54.444 90 41 0 1 90 502 591 2.88e-29 109
Msa0420750 MtrunA17_Chr7g0227991 52.747 91 43 0 1 91 471 561 2.89e-29 109
Msa0420750 MtrunA17_Chr6g0470851 50.549 91 43 1 1 89 466 556 6.79e-29 108
Msa0420750 MtrunA17_Chr2g0285111 53.261 92 42 1 1 91 519 610 6.92e-29 108
Msa0420750 MtrunA17_Chr3g0134621 52.809 89 42 0 1 89 459 547 7.28e-29 108
Msa0420750 MtrunA17_Chr1g0146251 52.326 86 41 0 4 89 476 561 8.18e-29 108
Msa0420750 MtrunA17_Chr1g0170081 53.488 86 40 0 4 89 472 557 9.37e-29 108
Msa0420750 MtrunA17_Chr4g0070391 54.444 90 40 1 1 90 481 569 9.40e-29 108
Msa0420750 MtrunA17_Chr2g0285121 54.348 92 41 1 1 91 516 607 1.14e-28 108
Msa0420750 MtrunA17_Chr8g0376841 53.261 92 43 0 1 92 493 584 1.87e-28 107
Msa0420750 MtrunA17_Chr8g0344971 52.874 87 41 0 3 89 485 571 2.46e-28 107
Msa0420750 MtrunA17_Chr1g0146271 51.163 86 42 0 4 89 474 559 3.65e-28 106
Msa0420750 MtrunA17_Chr6g0470881 52.273 88 40 1 4 89 469 556 6.80e-28 105
Msa0420750 MtrunA17_Chr7g0262161 55.056 89 40 0 1 89 485 573 6.90e-28 105
Msa0420750 MtrunA17_Chr8g0334521 48.352 91 47 0 1 91 471 561 1.21e-27 105
Msa0420750 MtrunA17_Chr7g0246461 50.562 89 44 0 1 89 467 555 2.55e-27 104
Msa0420750 MtrunA17_Chr4g0035881 46.602 103 51 1 1 99 225 327 9.79e-27 100
Msa0420750 MtrunA17_Chr5g0407701 54.545 88 32 1 2 89 494 573 1.06e-26 102
Msa0420750 MtrunA17_Chr6g0480721 50.538 93 44 2 1 92 340 431 1.16e-26 102
Msa0420750 MtrunA17_Chr2g0301011 49.438 89 45 0 1 89 335 423 2.42e-26 100
Msa0420750 MtrunA17_Chr8g0346361 48.352 91 47 0 1 91 234 324 2.80e-26 100
Msa0420750 MtrunA17_Chr8g0351011 50.549 91 45 0 1 91 489 579 4.12e-26 100
Msa0420750 MtrunA17_Chr8g0334491 52.273 88 32 1 4 91 476 553 9.20e-26 99.8
Msa0420750 MtrunA17_Chr8g0369891 53.933 89 31 2 1 89 498 576 6.38e-25 97.4
Msa0420750 MtrunA17_Chr4g0026641 51.136 88 41 1 2 89 491 576 8.29e-25 97.1
Msa0420750 MtrunA17_Chr5g0398331 53.933 89 34 1 1 89 489 570 3.49e-24 95.1
Msa0420750 MtrunA17_Chr4g0057061 53.933 89 37 2 1 89 512 596 7.99e-24 94.4
Msa0420750 MtrunA17_Chr8g0377961 48.913 92 40 2 1 92 515 599 1.05e-23 94.0
Msa0420750 MtrunA17_Chr5g0398401 53.409 88 34 1 4 91 495 575 1.11e-23 94.0
Msa0420750 MtrunA17_Chr5g0398241 53.409 88 34 1 4 91 495 575 1.18e-23 94.0
Msa0420750 MtrunA17_Chr5g0398351 53.488 86 33 1 4 89 475 553 1.73e-23 93.2
Msa0420750 MtrunA17_Chr5g0398351 52.326 86 34 1 4 89 1246 1324 6.41e-23 91.7
Msa0420750 MtrunA17_Chr3g0143231 47.778 90 46 1 1 90 2 90 3.89e-23 85.1
Msa0420750 MtrunA17_Chr7g0246451 44.444 90 50 0 2 91 483 572 6.19e-23 91.7
Msa0420750 MtrunA17_Chr3g0079971 47.126 87 44 2 5 91 473 557 6.31e-23 91.7
Msa0420750 MtrunA17_Chr5g0398311 51.685 89 36 1 1 89 485 566 7.82e-23 91.3
Msa0420750 MtrunA17_Chr1g0189251 50.549 91 35 3 4 89 486 571 1.06e-22 90.9
Msa0420750 MtrunA17_Chr2g0312861 48.000 75 39 0 17 91 1 75 6.88e-22 87.0
Msa0420750 MtrunA17_Chr2g0303881 48.352 91 38 2 3 89 458 543 9.31e-22 88.2
Msa0420750 MtrunA17_Chr7g0266051 47.191 89 47 0 1 89 442 530 1.17e-21 88.2
Msa0420750 MtrunA17_Chr2g0301031 48.649 74 38 0 17 90 1 74 1.31e-21 85.5
Msa0420750 MtrunA17_Chr8g0345991 47.826 92 38 3 3 89 484 570 1.62e-21 87.8
Msa0420750 MtrunA17_Chr3g0136151 46.739 92 48 1 1 92 490 580 1.72e-21 87.4
Msa0420750 MtrunA17_Chr1g0189561 51.064 94 39 2 2 91 487 577 2.13e-21 87.4
Msa0420750 MtrunA17_Chr8g0354741 47.191 89 45 1 1 89 368 454 2.27e-21 87.4
Msa0420750 MtrunA17_Chr3g0105451 45.652 92 37 1 1 92 19 97 5.22e-21 84.3
Msa0420750 MtrunA17_Chr8g0345971 47.872 94 33 3 4 89 490 575 6.85e-21 85.9
Msa0420750 MtrunA17_Chr5g0418921 46.739 92 39 2 3 89 18 104 3.57e-20 80.1
Msa0420750 MtrunA17_Chr3g0143251 45.055 91 49 1 2 92 495 584 3.90e-20 83.6
Msa0420750 MtrunA17_Chr8g0345981 43.269 104 44 4 3 96 488 586 4.97e-20 83.6
Msa0420750 MtrunA17_Chr8g0354771 42.222 90 51 1 1 89 495 584 1.24e-19 82.4
Msa0420750 MtrunA17_Chr7g0248871 46.739 92 46 2 2 91 493 583 1.90e-19 81.6
Msa0420750 MtrunA17_Chr4g0071191 42.222 90 50 2 4 92 63 151 6.74e-19 78.2
Msa0420750 MtrunA17_Chr7g0276151 45.455 88 45 2 4 89 486 572 1.04e-18 79.7
Msa0420750 MtrunA17_Chr4g0026661 45.205 73 38 1 17 89 12 82 1.49e-18 77.8
Msa0420750 MtrunA17_Chr7g0248861 46.067 89 47 1 2 89 495 583 4.06e-18 78.2
Msa0420750 MtrunA17_Chr4g0071871 45.745 94 43 2 1 89 542 632 7.18e-18 77.4
Msa0420750 MtrunA17_Chr3g0142971 42.353 85 48 1 1 85 2 85 1.83e-17 70.9
Msa0420750 MtrunA17_Chr4g0075331 43.750 96 42 3 4 92 471 561 2.14e-16 73.2
Msa0420750 MtrunA17_Chr8g0345961 48.052 77 39 1 13 89 498 573 7.76e-16 71.6
Msa0420750 MtrunA17_Chr2g0298601 42.391 92 41 3 1 85 542 628 1.71e-15 70.5
Msa0420750 MtrunA17_Chr8g0380961 36.842 95 46 3 4 90 576 664 5.70e-14 66.2
Msa0420750 MtrunA17_Chr2g0302951 36.082 97 48 3 4 92 568 658 3.62e-13 63.9
Msa0420750 MtrunA17_Chr3g0097061 43.396 53 30 0 39 91 321 373 2.63e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0420750 AT2G04160.1 74.157 89 23 0 1 89 505 593 4.69e-39 137
Msa0420750 AT5G59810.1 68.817 93 29 0 1 93 511 603 1.23e-37 134
Msa0420750 AT5G58840.3 51.000 100 49 0 1 100 443 542 6.36e-33 119
Msa0420750 AT5G67360.1 57.609 92 39 0 1 92 486 577 7.69e-33 120
Msa0420750 AT2G05920.1 58.889 90 37 0 1 90 480 569 1.21e-32 119
Msa0420750 AT3G14067.1 58.427 89 37 0 1 89 490 578 3.71e-31 115
Msa0420750 AT5G59100.1 53.933 89 41 0 1 89 472 560 5.32e-31 115
Msa0420750 AT5G59090.2 54.839 93 40 1 1 93 465 555 5.78e-31 115
Msa0420750 AT5G58840.1 53.933 89 41 0 1 89 443 531 8.55e-31 114
Msa0420750 AT5G58820.1 52.809 89 42 0 1 89 439 527 3.33e-30 112
Msa0420750 AT5G59090.3 56.180 89 37 1 1 89 463 549 3.40e-30 112
Msa0420750 AT5G59090.1 56.180 89 37 1 1 89 465 551 3.47e-30 112
Msa0420750 AT5G58830.1 51.685 89 43 0 1 89 442 530 1.45e-29 110
Msa0420750 AT1G04110.1 56.180 89 39 0 1 89 496 584 2.72e-29 110
Msa0420750 AT5G59120.1 55.056 89 38 1 1 89 464 550 3.62e-29 109
Msa0420750 AT5G51750.1 56.180 89 39 0 1 89 504 592 4.61e-29 109
Msa0420750 AT4G34980.1 55.056 89 40 0 1 89 482 570 9.16e-29 108
Msa0420750 AT3G14240.1 52.222 90 43 0 1 90 493 582 5.59e-28 106
Msa0420750 AT3G46840.1 51.685 89 41 1 1 89 473 559 4.62e-27 103
Msa0420750 AT5G45650.2 50.562 89 44 0 1 89 522 610 9.27e-27 103
Msa0420750 AT5G45650.1 50.562 89 44 0 1 89 522 610 9.27e-27 103
Msa0420750 AT1G01900.1 51.648 91 44 0 1 91 496 586 1.09e-26 102
Msa0420750 AT4G26330.1 48.515 101 51 1 1 100 464 564 6.76e-26 100
Msa0420750 AT4G26330.2 48.515 101 51 1 1 100 450 550 7.09e-26 100
Msa0420750 AT3G46850.1 48.315 89 44 1 1 89 473 559 2.28e-25 99.0
Msa0420750 AT5G03620.2 50.000 88 44 0 4 91 490 577 4.59e-25 98.2
Msa0420750 AT5G59190.1 44.792 96 49 2 4 96 430 524 9.53e-25 97.4
Msa0420750 AT4G00230.2 46.237 93 50 0 1 93 480 572 1.06e-24 97.1
Msa0420750 AT5G03620.1 50.000 86 43 0 4 89 490 575 3.50e-24 95.5
Msa0420750 AT4G00230.1 47.253 91 48 0 1 91 480 570 4.96e-24 95.1
Msa0420750 AT4G10540.1 56.180 89 32 2 1 89 504 585 5.32e-24 95.1
Msa0420750 AT4G10550.1 54.348 92 34 2 1 92 508 591 9.72e-24 94.4
Msa0420750 AT4G10550.4 54.348 92 34 2 1 92 452 535 1.15e-23 94.4
Msa0420750 AT4G10550.2 54.348 92 34 2 1 92 452 535 1.15e-23 94.4
Msa0420750 AT4G21650.1 55.056 89 33 2 1 89 500 581 1.16e-23 94.4
Msa0420750 AT4G10550.3 54.348 92 34 2 1 92 524 607 1.16e-23 94.4
Msa0420750 AT5G59130.1 51.163 86 39 1 4 89 464 546 3.56e-23 92.8
Msa0420750 AT5G59130.4 51.163 86 39 1 4 89 468 550 3.70e-23 92.8
Msa0420750 AT5G59130.2 51.163 86 39 1 4 89 458 540 3.81e-23 92.8
Msa0420750 AT5G59130.3 51.163 86 39 1 4 89 429 511 3.95e-23 92.8
Msa0420750 AT1G20160.2 48.315 89 45 1 1 89 452 539 1.82e-22 90.9
Msa0420750 AT1G20160.1 48.315 89 45 1 1 89 491 578 1.96e-22 90.9
Msa0420750 AT5G67090.1 49.438 89 45 0 1 89 473 561 2.37e-22 90.5
Msa0420750 AT1G32950.1 53.933 89 34 2 1 89 502 583 4.30e-22 89.7
Msa0420750 AT1G32950.2 53.933 89 34 2 1 89 451 532 4.58e-22 89.7
Msa0420750 AT4G10520.1 48.913 92 40 2 1 92 485 569 5.02e-22 89.4
Msa0420750 AT4G21630.1 52.809 89 35 2 1 89 493 574 6.65e-22 89.4
Msa0420750 AT1G32970.1 50.000 92 39 2 1 92 463 547 1.03e-21 88.6
Msa0420750 AT4G10510.2 55.056 89 33 2 1 89 435 516 2.06e-21 87.8
Msa0420750 AT4G10510.1 55.056 89 33 2 1 89 494 575 2.10e-21 87.8
Msa0420750 AT4G10510.3 55.056 89 33 2 1 89 506 587 2.10e-21 87.8
Msa0420750 AT4G10510.4 55.056 89 33 2 1 89 450 531 2.11e-21 87.8
Msa0420750 AT5G11940.1 48.315 89 39 1 1 89 491 572 3.00e-21 87.4
Msa0420750 AT1G20150.1 52.326 86 40 1 4 89 498 582 7.49e-21 86.3
Msa0420750 AT4G21640.1 52.809 89 35 2 1 89 467 548 1.02e-20 85.9
Msa0420750 AT1G32960.1 52.326 86 34 2 4 89 509 587 2.53e-20 84.7
Msa0420750 AT1G66220.1 47.191 89 33 1 4 92 495 569 2.78e-20 84.7
Msa0420750 AT4G21326.1 48.315 89 39 1 4 92 431 512 1.50e-19 82.4
Msa0420750 AT2G39850.1 43.434 99 45 4 1 92 491 585 2.12e-19 82.0
Msa0420750 AT5G45640.1 47.222 72 38 0 19 90 502 573 2.73e-19 81.6
Msa0420750 AT1G66210.1 50.562 89 32 2 1 89 495 571 3.99e-19 81.3
Msa0420750 AT1G32940.3 51.111 90 37 1 1 90 503 585 6.18e-19 80.9
Msa0420750 AT2G19170.1 47.368 95 40 3 1 89 538 628 1.05e-18 80.1
Msa0420750 AT2G19170.2 47.368 95 40 3 1 89 538 628 1.05e-18 80.1
Msa0420750 AT4G30020.3 45.745 94 43 2 1 89 539 629 2.28e-18 79.0
Msa0420750 AT4G30020.1 45.745 94 43 2 1 89 539 629 2.28e-18 79.0
Msa0420750 AT4G30020.4 45.745 94 43 2 1 89 539 629 2.28e-18 79.0
Msa0420750 AT4G30020.2 45.745 94 43 2 1 89 539 629 2.28e-18 79.0
Msa0420750 AT1G32940.1 50.562 89 37 1 1 89 503 584 4.90e-18 78.2
Msa0420750 AT1G32940.4 50.562 89 37 1 1 89 452 533 5.22e-18 78.2
Msa0420750 AT1G32940.2 50.562 89 37 1 1 89 452 533 5.22e-18 78.2
Msa0420750 AT4G21323.1 43.182 88 41 1 5 92 537 615 7.21e-16 72.0
Msa0420750 AT4G15040.2 37.931 87 53 1 4 89 426 512 8.85e-15 68.9
Msa0420750 AT4G15040.1 37.931 87 53 1 4 89 426 512 8.85e-15 68.9
Msa0420750 AT1G62340.1 43.750 96 42 4 4 92 563 653 2.03e-14 67.8
Msa0420750 AT1G30600.1 38.462 91 48 2 4 89 554 641 2.16e-13 65.1
Msa0420750 AT5G44530.2 35.106 94 47 3 4 89 564 651 7.38e-13 63.5
Msa0420750 AT5G44530.1 35.106 94 47 3 4 89 564 651 7.96e-13 63.2
Msa0420750 AT4G20430.3 35.165 91 51 2 4 89 579 666 1.09e-11 60.1
Msa0420750 AT4G20430.1 35.165 91 51 2 4 89 579 666 1.09e-11 60.1
Msa0420750 AT4G20430.2 35.165 91 51 2 4 89 555 642 1.10e-11 60.1

Find 103 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGCAAAATTTGATAATTT+TGG 0.086768 3_2:-69475290 Msa0420750:five_prime_UTR
GCATCTGTTGCTTTAATAAT+TGG 0.089925 3_2:+69474548 None:intergenic
TAAAGCAACAGATGCTAAAT+TGG 0.221704 3_2:-69474540 Msa0420750:five_prime_UTR
TGAGAACCTTGTATCCTTCT+TGG 0.224032 3_2:-69473707 Msa0420750:CDS
ATTGTTGGTTATTTGGAAAT+TGG 0.236564 3_2:-69475068 Msa0420750:five_prime_UTR
TTCACTAGTTATGTTCTTCT+TGG 0.240053 3_2:-69474800 Msa0420750:five_prime_UTR
GTTGTTGTTGCTTGCTCTGC+TGG 0.279959 3_2:-69474905 Msa0420750:five_prime_UTR
ATCTCGGGTACAATGGTATT+TGG 0.284686 3_2:+69473982 None:intergenic
TGACTCTCAATTATCTTGTT+TGG 0.307210 3_2:-69474624 Msa0420750:five_prime_UTR
TGTGATCCAGAGTCCCATTC+TGG 0.311076 3_2:+69474388 None:intergenic
AGTCTGAAGTTGAGATTTCT+TGG 0.315236 3_2:+69473597 None:intergenic
CATGTCAATCCCTCACATTT+CGG 0.322605 3_2:-69473745 Msa0420750:CDS
CCTCACATTTCGGGAATTGC+AGG 0.324105 3_2:-69473735 Msa0420750:CDS
GGTTATTTGGAAATTGGTTT+TGG 0.327450 3_2:-69475062 Msa0420750:five_prime_UTR
TACAATGGTATTTGGCCCTT+TGG 0.331318 3_2:+69473990 None:intergenic
AAATCATTTCATAAATATTC+CGG 0.336856 3_2:-69474946 Msa0420750:five_prime_UTR
ATGTCAATCCCTCACATTTC+GGG 0.337975 3_2:-69473744 Msa0420750:CDS
ATTTCCACTATTGTTATCAT+TGG 0.342216 3_2:+69474272 None:intergenic
GCTTGCTCTGCTGGCAATGA+TGG 0.343896 3_2:-69474896 Msa0420750:five_prime_UTR
CCTTATCCACTCCTCCATTC+AGG 0.352074 3_2:+69474337 None:intergenic
TTTCTATCAACCAATGTGAT+TGG 0.355031 3_2:+69475118 None:intergenic
GGAAGAACAGAAAGCCAATT+GGG 0.382375 3_2:+69475166 None:intergenic
CATCTGTTGCTTTAATAATT+GGG 0.387808 3_2:+69474549 None:intergenic
TTACTGTAATTTCTATCAAT+AGG 0.388983 3_2:-69474698 Msa0420750:five_prime_UTR
TTGTCGATCTCTTCATTAGT+TGG 0.405128 3_2:+69473804 None:intergenic
ACACCTGGTGCTGTGATATC+AGG 0.406349 3_2:+69473861 None:intergenic
ACCCAGAAAAGCTCAGTTCA+TGG 0.406753 3_2:-69475309 Msa0420750:five_prime_UTR
TGGCAACGGTTTAAGCTTCA+AGG 0.407648 3_2:-69474780 Msa0420750:five_prime_UTR
CTTGCCAATGATAACAATAG+TGG 0.413339 3_2:-69474276 Msa0420750:five_prime_UTR
TTTGATGCAGTAGCCTCATT+TGG 0.413957 3_2:+69474872 None:intergenic
TCTTGTTTGGTTCAATCAAC+GGG 0.414439 3_2:-69474611 Msa0420750:five_prime_UTR
AATATATCATCAAAAGAAGT+TGG 0.416216 3_2:+69474734 None:intergenic
TGATCAAGAATGAGATTTGT+GGG 0.416911 3_2:+69475366 None:intergenic
TTCTATCAACCAATGTGATT+GGG 0.424736 3_2:+69475119 None:intergenic
TCAAAGAGAAGTTGATCAAA+TGG 0.426603 3_2:-69473575 Msa0420750:three_prime_UTR
CAACAACAATATTTGTAAAC+CGG 0.431164 3_2:+69474927 None:intergenic
GAGAAATTTATGTTAGAAGC+AGG 0.435055 3_2:+69474222 None:intergenic
TGCAGCGAGCTTTGCCAGAA+TGG 0.435817 3_2:-69474402 Msa0420750:intron
CTTGTATCCTTCTTGGAGTC+CGG 0.441288 3_2:-69473700 Msa0420750:CDS
GAATGAGATTTGTGGGATTG+AGG 0.442758 3_2:+69475373 None:intergenic
TGAGTAAGACAAATTGAATT+CGG 0.461464 3_2:+69473778 None:intergenic
GTCTGAAGTTGAGATTTCTT+GGG 0.461732 3_2:+69473598 None:intergenic
GATTAACACCTTTAGAATCT+CGG 0.462819 3_2:+69473966 None:intergenic
ATCTTGTTTGGTTCAATCAA+CGG 0.466097 3_2:-69474612 Msa0420750:five_prime_UTR
AATTAGAATAGTTTGAAAAG+TGG 0.469091 3_2:+69473541 None:intergenic
AGGAAGAACAGAAAGCCAAT+TGG 0.469166 3_2:+69475165 None:intergenic
TTTGTGGGATTGAGGAATTG+AGG 0.470007 3_2:+69475381 None:intergenic
CCTGAATGGAGGAGTGGATA+AGG 0.471847 3_2:-69474337 Msa0420750:five_prime_UTR
GAATTGGTTGTTGGAGATGA+AGG 0.478589 3_2:-69475040 Msa0420750:five_prime_UTR
TCGAAAAGAGAAGAATGAAA+AGG 0.479236 3_2:-69475013 Msa0420750:five_prime_UTR
TTGATCAAGAATGAGATTTG+TGG 0.480475 3_2:+69475365 None:intergenic
AGGAGTGGATAAGGGAGAAC+AGG 0.483171 3_2:-69474328 Msa0420750:five_prime_UTR
AAAATAGTAGTGTGCCTGAA+TGG 0.485193 3_2:-69474351 Msa0420750:five_prime_UTR
GCAGCGAGCTTTGCCAGAAT+GGG 0.489122 3_2:-69474401 Msa0420750:intron
ATTAACACCTTTAGAATCTC+GGG 0.499143 3_2:+69473967 None:intergenic
GGATGAAAATATTGCCATGA+TGG 0.505910 3_2:+69474011 None:intergenic
CACTATTGTTATCATTGGCA+AGG 0.506923 3_2:+69474277 None:intergenic
AAATTGAGATACCTACCAAA+TGG 0.508336 3_2:-69475433 Msa0420750:five_prime_UTR
CAGCCTGATATCACAGCACC+AGG 0.512049 3_2:-69473864 Msa0420750:CDS
TCTAACATAAATTTCTCTGA+TGG 0.516359 3_2:-69474216 Msa0420750:five_prime_UTR
CTGCATCAAATCTAGCTCCT+TGG 0.516497 3_2:-69474859 Msa0420750:five_prime_UTR
CTGAATGGAGGAGTGGATAA+GGG 0.525356 3_2:-69474336 Msa0420750:five_prime_UTR
GAACTCTCTATCCATTGTGC+TGG 0.525429 3_2:+69474820 None:intergenic
AGTTATGTTCTTCTTGGCAA+CGG 0.530911 3_2:-69474794 Msa0420750:five_prime_UTR
AAGGATACAAGGTTCTCAAA+AGG 0.532636 3_2:+69473712 None:intergenic
GGAGAACAGGCTCTTATAGC+TGG 0.534606 3_2:-69474315 Msa0420750:five_prime_UTR
ATTCAATTTGTCTTACTCAC+CGG 0.545243 3_2:-69473774 Msa0420750:CDS
GCTTTGCCAGAATGGGACTC+TGG 0.545631 3_2:-69474394 Msa0420750:five_prime_UTR
CATTGTACCCGAGATTCTAA+AGG 0.548172 3_2:-69473974 Msa0420750:intron
GCTCTTATAGCTGGTGCTGT+AGG 0.550029 3_2:-69474306 Msa0420750:five_prime_UTR
AGAATAGTTTGAAAAGTGGA+AGG 0.552146 3_2:+69473545 None:intergenic
TGGCTCTTCAGCTTCAGTGA+AGG 0.552957 3_2:+69473824 None:intergenic
GCTCCTTGGTATATCACAGT+TGG 0.557076 3_2:-69474845 Msa0420750:five_prime_UTR
GATGAAAATATTGCCATGAT+GGG 0.560543 3_2:+69474012 None:intergenic
ATTGTTATTCCCAATCACAT+TGG 0.561112 3_2:-69475128 Msa0420750:five_prime_UTR
TATAGCTGGTGCTGTAGGAG+TGG 0.566698 3_2:-69474301 Msa0420750:five_prime_UTR
ATAGCAGATTTAATAGCAGC+CGG 0.569981 3_2:+69473681 None:intergenic
CAAGAGTTTCTTCTTACCTG+TGG 0.573388 3_2:+69473653 None:intergenic
AGGGATTGACATGGATGTGC+CGG 0.584068 3_2:+69473755 None:intergenic
CGGACTCCAAGAAGGATACA+AGG 0.588458 3_2:+69473701 None:intergenic
CTTTAGAATCTCGGGTACAA+TGG 0.591821 3_2:+69473975 None:intergenic
AAATCTGCTATCATGACCAC+AGG 0.593204 3_2:-69473669 Msa0420750:CDS
CTGCAATTCCCGAAATGTGA+GGG 0.595072 3_2:+69473736 None:intergenic
TGGCAATGATGGACCAAATG+AGG 0.596925 3_2:-69474885 Msa0420750:five_prime_UTR
CACAGTTGGTGCCAGCACAA+TGG 0.602894 3_2:-69474831 Msa0420750:five_prime_UTR
ATAGCAGCCGGACTCCAAGA+AGG 0.603386 3_2:+69473693 None:intergenic
TCTGGATCACAAGAAGGTGA+AGG 0.614201 3_2:-69474376 Msa0420750:five_prime_UTR
TGGGACTCTGGATCACAAGA+AGG 0.633879 3_2:-69474382 Msa0420750:five_prime_UTR
CTGGATCACAAGAAGGTGAA+GGG 0.633941 3_2:-69474375 Msa0420750:five_prime_UTR
GCTGCTATAACCGACACACC+TGG 0.638678 3_2:+69473846 None:intergenic
CGAAATGTGAGGGATTGACA+TGG 0.639293 3_2:+69473746 None:intergenic
GAAGAACAGAAAGCCAATTG+GGG 0.645538 3_2:+69475167 None:intergenic
TTAGAAGCAGGAAGAACATG+AGG 0.651996 3_2:+69474234 None:intergenic
ATAGTAGTGTGCCTGAATGG+AGG 0.653182 3_2:-69474348 Msa0420750:five_prime_UTR
GGATGTGTAATATAAGCCAC+TGG 0.654484 3_2:+69474063 None:intergenic
CTTGTTTGGTTCAATCAACG+GGG 0.655523 3_2:-69474610 Msa0420750:five_prime_UTR
TATCACAGCACCAGGTGTGT+CGG 0.657844 3_2:-69473856 Msa0420750:CDS
CCTGCAATTCCCGAAATGTG+AGG 0.660279 3_2:+69473735 None:intergenic
TGAAAATATTGCCATGATGG+GGG 0.660947 3_2:+69474014 None:intergenic
ATGAAAATATTGCCATGATG+GGG 0.674957 3_2:+69474013 None:intergenic
AGTGTGCCTGAATGGAGGAG+TGG 0.692602 3_2:-69474343 Msa0420750:five_prime_UTR
GCACCAACTGTGATATACCA+AGG 0.694138 3_2:+69474842 None:intergenic
TTGTTTGGTTCAATCAACGG+GGG 0.709692 3_2:-69474609 Msa0420750:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGTTTTTCAATTAAAAAT+TGG - chr3_2:69473704-69473723 Msa0420750:CDS 10.0%
!!! ATTTTTAATTGAAAACTAAA+AGG + chr3_2:69473731-69473750 None:intergenic 10.0%
!!! TTTTAGTTTTCAATTAAAAA+TGG - chr3_2:69473730-69473749 Msa0420750:CDS 10.0%
!! TTATTCATTACTAAAAAATT+TGG + chr3_2:69473860-69473879 None:intergenic 10.0%
!!! ACTAATGATATTATATTTTT+TGG + chr3_2:69474467-69474486 None:intergenic 10.0%
!!! TTAGTTTTTCAATTAAAAAT+TGG - chr3_2:69473704-69473723 Msa0420750:CDS 10.0%
!!! ATTTTTAATTGAAAACTAAA+AGG + chr3_2:69473731-69473750 None:intergenic 10.0%
!!! TTTTAGTTTTCAATTAAAAA+TGG - chr3_2:69473730-69473749 Msa0420750:CDS 10.0%
!! TTATTCATTACTAAAAAATT+TGG + chr3_2:69473860-69473879 None:intergenic 10.0%
!!! ACTAATGATATTATATTTTT+TGG + chr3_2:69474467-69474486 None:intergenic 10.0%
!!! TTTTCAATTAAAAATGGATT+AGG - chr3_2:69473736-69473755 Msa0420750:CDS 15.0%
!! GTAATGAATAAAAAAATTGT+TGG - chr3_2:69473869-69473888 Msa0420750:intron 15.0%
!!! TAAAAAAATTGTTGGTTATT+TGG - chr3_2:69473877-69473896 Msa0420750:intron 15.0%
!! AAATCATTTCATAAATATTC+CGG - chr3_2:69474006-69474025 Msa0420750:CDS 15.0%
!!! TTTTCAATTAAAAATGGATT+AGG - chr3_2:69473736-69473755 Msa0420750:CDS 15.0%
!! GTAATGAATAAAAAAATTGT+TGG - chr3_2:69473869-69473888 Msa0420750:intron 15.0%
!!! TAAAAAAATTGTTGGTTATT+TGG - chr3_2:69473877-69473896 Msa0420750:intron 15.0%
!! AAATCATTTCATAAATATTC+CGG - chr3_2:69474006-69474025 Msa0420750:CDS 15.0%
!!! ATCTCAATTTTAATTTTTCC+CGG + chr3_2:69473509-69473528 None:intergenic 20.0%
!!! GAATTTTTATGTTATCCATT+TGG + chr3_2:69473537-69473556 None:intergenic 20.0%
!!! ATGGCAAAATTTGATAATTT+TGG - chr3_2:69473662-69473681 Msa0420750:CDS 20.0%
!!! TTGATAATTTTGGATATAGA+AGG - chr3_2:69473672-69473691 Msa0420750:CDS 20.0%
!! AACAATATGTAAGAGAAAAA+AGG + chr3_2:69473810-69473829 None:intergenic 20.0%
!! AATATATCATCAAAAGAAGT+TGG + chr3_2:69474221-69474240 None:intergenic 20.0%
!! TTACTGTAATTTCTATCAAT+AGG - chr3_2:69474254-69474273 Msa0420750:five_prime_UTR 20.0%
!! AATTAGAATAGTTTGAAAAG+TGG + chr3_2:69475414-69475433 None:intergenic 20.0%
!!! ATCTCAATTTTAATTTTTCC+CGG + chr3_2:69473509-69473528 None:intergenic 20.0%
!!! GAATTTTTATGTTATCCATT+TGG + chr3_2:69473537-69473556 None:intergenic 20.0%
!!! ATGGCAAAATTTGATAATTT+TGG - chr3_2:69473662-69473681 Msa0420750:CDS 20.0%
!!! TTGATAATTTTGGATATAGA+AGG - chr3_2:69473672-69473691 Msa0420750:CDS 20.0%
!! AACAATATGTAAGAGAAAAA+AGG + chr3_2:69473810-69473829 None:intergenic 20.0%
!! AATATATCATCAAAAGAAGT+TGG + chr3_2:69474221-69474240 None:intergenic 20.0%
!! TTACTGTAATTTCTATCAAT+AGG - chr3_2:69474254-69474273 Msa0420750:five_prime_UTR 20.0%
!! AATTAGAATAGTTTGAAAAG+TGG + chr3_2:69475414-69475433 None:intergenic 20.0%
!! TTTTATGTTATCCATTTGGT+AGG + chr3_2:69473533-69473552 None:intergenic 25.0%
!! TTTTGGATATAGAAGGAAAA+AGG - chr3_2:69473679-69473698 Msa0420750:CDS 25.0%
! TTTGGATATAGAAGGAAAAA+GGG - chr3_2:69473680-69473699 Msa0420750:CDS 25.0%
!! ATTGTTGGTTATTTGGAAAT+TGG - chr3_2:69473884-69473903 Msa0420750:intron 25.0%
!! ATGCATTTTGTTAGAATTTC+TGG - chr3_2:69473983-69474002 Msa0420750:CDS 25.0%
! CAACAACAATATTTGTAAAC+CGG + chr3_2:69474028-69474047 None:intergenic 25.0%
!! TAATTGGGTAGAATTTTTGT+GGG + chr3_2:69474391-69474410 None:intergenic 25.0%
!! ATAATTGGGTAGAATTTTTG+TGG + chr3_2:69474392-69474411 None:intergenic 25.0%
!!! CATCTGTTGCTTTAATAATT+GGG + chr3_2:69474406-69474425 None:intergenic 25.0%
! TCAATTAGATCAAGTTACAT+TGG + chr3_2:69474526-69474545 None:intergenic 25.0%
! ATTTCCACTATTGTTATCAT+TGG + chr3_2:69474683-69474702 None:intergenic 25.0%
! TCTAACATAAATTTCTCTGA+TGG - chr3_2:69474736-69474755 Msa0420750:five_prime_UTR 25.0%
!!! TTAGTATGCAATTTTGTTGT+TGG + chr3_2:69474913-69474932 None:intergenic 25.0%
! ACATAACAATAGATTACAAG+AGG + chr3_2:69475036-69475055 None:intergenic 25.0%
! TGAGTAAGACAAATTGAATT+CGG + chr3_2:69475177-69475196 None:intergenic 25.0%
!! TTTTATGTTATCCATTTGGT+AGG + chr3_2:69473533-69473552 None:intergenic 25.0%
!! TTTTGGATATAGAAGGAAAA+AGG - chr3_2:69473679-69473698 Msa0420750:CDS 25.0%
! TTTGGATATAGAAGGAAAAA+GGG - chr3_2:69473680-69473699 Msa0420750:CDS 25.0%
!! ATTGTTGGTTATTTGGAAAT+TGG - chr3_2:69473884-69473903 Msa0420750:intron 25.0%
!! ATGCATTTTGTTAGAATTTC+TGG - chr3_2:69473983-69474002 Msa0420750:CDS 25.0%
! CAACAACAATATTTGTAAAC+CGG + chr3_2:69474028-69474047 None:intergenic 25.0%
!! TAATTGGGTAGAATTTTTGT+GGG + chr3_2:69474391-69474410 None:intergenic 25.0%
!! ATAATTGGGTAGAATTTTTG+TGG + chr3_2:69474392-69474411 None:intergenic 25.0%
!!! CATCTGTTGCTTTAATAATT+GGG + chr3_2:69474406-69474425 None:intergenic 25.0%
! TCAATTAGATCAAGTTACAT+TGG + chr3_2:69474526-69474545 None:intergenic 25.0%
! ATTTCCACTATTGTTATCAT+TGG + chr3_2:69474683-69474702 None:intergenic 25.0%
! TCTAACATAAATTTCTCTGA+TGG - chr3_2:69474736-69474755 Msa0420750:five_prime_UTR 25.0%
!!! TTAGTATGCAATTTTGTTGT+TGG + chr3_2:69474913-69474932 None:intergenic 25.0%
! ACATAACAATAGATTACAAG+AGG + chr3_2:69475036-69475055 None:intergenic 25.0%
! TGAGTAAGACAAATTGAATT+CGG + chr3_2:69475177-69475196 None:intergenic 25.0%
AAATTGAGATACCTACCAAA+TGG - chr3_2:69473519-69473538 Msa0420750:three_prime_UTR 30.0%
TGATCAAGAATGAGATTTGT+GGG + chr3_2:69473589-69473608 None:intergenic 30.0%
TTGATCAAGAATGAGATTTG+TGG + chr3_2:69473590-69473609 None:intergenic 30.0%
TTGGATATAGAAGGAAAAAG+GGG - chr3_2:69473681-69473700 Msa0420750:CDS 30.0%
!!! TTCTTTTTTAACACCCCAAT+TGG - chr3_2:69473772-69473791 Msa0420750:CDS 30.0%
! ATTGTTATTCCCAATCACAT+TGG - chr3_2:69473824-69473843 Msa0420750:CDS 30.0%
TTCTATCAACCAATGTGATT+GGG + chr3_2:69473836-69473855 None:intergenic 30.0%
TTTCTATCAACCAATGTGAT+TGG + chr3_2:69473837-69473856 None:intergenic 30.0%
!! GGTTATTTGGAAATTGGTTT+TGG - chr3_2:69473890-69473909 Msa0420750:intron 30.0%
!!! TTGGAAATTGGTTTTGGAAT+TGG - chr3_2:69473896-69473915 Msa0420750:intron 30.0%
TCGAAAAGAGAAGAATGAAA+AGG - chr3_2:69473939-69473958 Msa0420750:intron 30.0%
TTCACTAGTTATGTTCTTCT+TGG - chr3_2:69474152-69474171 Msa0420750:intron 30.0%
TGACTCTCAATTATCTTGTT+TGG - chr3_2:69474328-69474347 Msa0420750:five_prime_UTR 30.0%
! ATCTTGTTTGGTTCAATCAA+CGG - chr3_2:69474340-69474359 Msa0420750:five_prime_UTR 30.0%
!! GCATCTGTTGCTTTAATAAT+TGG + chr3_2:69474407-69474426 None:intergenic 30.0%
TAAAGCAACAGATGCTAAAT+TGG - chr3_2:69474412-69474431 Msa0420750:intron 30.0%
GAGAAATTTATGTTAGAAGC+AGG + chr3_2:69474733-69474752 None:intergenic 30.0%
ACACTTAAAACTTGATCGTT+TGG - chr3_2:69474849-69474868 Msa0420750:five_prime_UTR 30.0%
! ATCGTTTGGACTTAACTTTT+AGG - chr3_2:69474863-69474882 Msa0420750:five_prime_UTR 30.0%
ATGAAAATATTGCCATGATG+GGG + chr3_2:69474942-69474961 None:intergenic 30.0%
GATGAAAATATTGCCATGAT+GGG + chr3_2:69474943-69474962 None:intergenic 30.0%
! ATGGCAATATTTTCATCCAA+AGG - chr3_2:69474946-69474965 Msa0420750:five_prime_UTR 30.0%
! TGGCAATATTTTCATCCAAA+GGG - chr3_2:69474947-69474966 Msa0420750:five_prime_UTR 30.0%
!! ATTAACACCTTTAGAATCTC+GGG + chr3_2:69474988-69475007 None:intergenic 30.0%
!! GATTAACACCTTTAGAATCT+CGG + chr3_2:69474989-69475008 None:intergenic 30.0%
ATTCAATTTGTCTTACTCAC+CGG - chr3_2:69475178-69475197 Msa0420750:five_prime_UTR 30.0%
! TCAAAGAGAAGTTGATCAAA+TGG - chr3_2:69475377-69475396 Msa0420750:five_prime_UTR 30.0%
AGAATAGTTTGAAAAGTGGA+AGG + chr3_2:69475410-69475429 None:intergenic 30.0%
AAATTGAGATACCTACCAAA+TGG - chr3_2:69473519-69473538 Msa0420750:three_prime_UTR 30.0%
TGATCAAGAATGAGATTTGT+GGG + chr3_2:69473589-69473608 None:intergenic 30.0%
TTGATCAAGAATGAGATTTG+TGG + chr3_2:69473590-69473609 None:intergenic 30.0%
TTGGATATAGAAGGAAAAAG+GGG - chr3_2:69473681-69473700 Msa0420750:CDS 30.0%
!!! TTCTTTTTTAACACCCCAAT+TGG - chr3_2:69473772-69473791 Msa0420750:CDS 30.0%
! ATTGTTATTCCCAATCACAT+TGG - chr3_2:69473824-69473843 Msa0420750:CDS 30.0%
TTCTATCAACCAATGTGATT+GGG + chr3_2:69473836-69473855 None:intergenic 30.0%
TTTCTATCAACCAATGTGAT+TGG + chr3_2:69473837-69473856 None:intergenic 30.0%
!! GGTTATTTGGAAATTGGTTT+TGG - chr3_2:69473890-69473909 Msa0420750:intron 30.0%
!!! TTGGAAATTGGTTTTGGAAT+TGG - chr3_2:69473896-69473915 Msa0420750:intron 30.0%
TCGAAAAGAGAAGAATGAAA+AGG - chr3_2:69473939-69473958 Msa0420750:intron 30.0%
TTCACTAGTTATGTTCTTCT+TGG - chr3_2:69474152-69474171 Msa0420750:intron 30.0%
TGACTCTCAATTATCTTGTT+TGG - chr3_2:69474328-69474347 Msa0420750:five_prime_UTR 30.0%
! ATCTTGTTTGGTTCAATCAA+CGG - chr3_2:69474340-69474359 Msa0420750:five_prime_UTR 30.0%
!! GCATCTGTTGCTTTAATAAT+TGG + chr3_2:69474407-69474426 None:intergenic 30.0%
TAAAGCAACAGATGCTAAAT+TGG - chr3_2:69474412-69474431 Msa0420750:intron 30.0%
GAGAAATTTATGTTAGAAGC+AGG + chr3_2:69474733-69474752 None:intergenic 30.0%
ACACTTAAAACTTGATCGTT+TGG - chr3_2:69474849-69474868 Msa0420750:five_prime_UTR 30.0%
! ATCGTTTGGACTTAACTTTT+AGG - chr3_2:69474863-69474882 Msa0420750:five_prime_UTR 30.0%
ATGAAAATATTGCCATGATG+GGG + chr3_2:69474942-69474961 None:intergenic 30.0%
GATGAAAATATTGCCATGAT+GGG + chr3_2:69474943-69474962 None:intergenic 30.0%
! ATGGCAATATTTTCATCCAA+AGG - chr3_2:69474946-69474965 Msa0420750:five_prime_UTR 30.0%
! TGGCAATATTTTCATCCAAA+GGG - chr3_2:69474947-69474966 Msa0420750:five_prime_UTR 30.0%
!! ATTAACACCTTTAGAATCTC+GGG + chr3_2:69474988-69475007 None:intergenic 30.0%
!! GATTAACACCTTTAGAATCT+CGG + chr3_2:69474989-69475008 None:intergenic 30.0%
ATTCAATTTGTCTTACTCAC+CGG - chr3_2:69475178-69475197 Msa0420750:five_prime_UTR 30.0%
! TCAAAGAGAAGTTGATCAAA+TGG - chr3_2:69475377-69475396 Msa0420750:five_prime_UTR 30.0%
AGAATAGTTTGAAAAGTGGA+AGG + chr3_2:69475410-69475429 None:intergenic 30.0%
!!! TTGGTTTTGGAATTGGTTGT+TGG - chr3_2:69473903-69473922 Msa0420750:intron 35.0%
AGTTATGTTCTTCTTGGCAA+CGG - chr3_2:69474158-69474177 Msa0420750:intron 35.0%
! TCTTGTTTGGTTCAATCAAC+GGG - chr3_2:69474341-69474360 Msa0420750:five_prime_UTR 35.0%
AAAATAGTAGTGTGCCTGAA+TGG - chr3_2:69474601-69474620 Msa0420750:five_prime_UTR 35.0%
CACTATTGTTATCATTGGCA+AGG + chr3_2:69474678-69474697 None:intergenic 35.0%
CTTGCCAATGATAACAATAG+TGG - chr3_2:69474676-69474695 Msa0420750:five_prime_UTR 35.0%
AGAAATCTAGCAGTCTAGTT+TGG - chr3_2:69474795-69474814 Msa0420750:five_prime_UTR 35.0%
TGAAAATATTGCCATGATGG+GGG + chr3_2:69474941-69474960 None:intergenic 35.0%
GGATGAAAATATTGCCATGA+TGG + chr3_2:69474944-69474963 None:intergenic 35.0%
TTGTCGATCTCTTCATTAGT+TGG + chr3_2:69475151-69475170 None:intergenic 35.0%
AAGGATACAAGGTTCTCAAA+AGG + chr3_2:69475243-69475262 None:intergenic 35.0%
ATAGCAGATTTAATAGCAGC+CGG + chr3_2:69475274-69475293 None:intergenic 35.0%
! GTCTGAAGTTGAGATTTCTT+GGG + chr3_2:69475357-69475376 None:intergenic 35.0%
! AGTCTGAAGTTGAGATTTCT+TGG + chr3_2:69475358-69475377 None:intergenic 35.0%
!!! TTGGTTTTGGAATTGGTTGT+TGG - chr3_2:69473903-69473922 Msa0420750:intron 35.0%
AGTTATGTTCTTCTTGGCAA+CGG - chr3_2:69474158-69474177 Msa0420750:intron 35.0%
! TCTTGTTTGGTTCAATCAAC+GGG - chr3_2:69474341-69474360 Msa0420750:five_prime_UTR 35.0%
AAAATAGTAGTGTGCCTGAA+TGG - chr3_2:69474601-69474620 Msa0420750:five_prime_UTR 35.0%
CACTATTGTTATCATTGGCA+AGG + chr3_2:69474678-69474697 None:intergenic 35.0%
CTTGCCAATGATAACAATAG+TGG - chr3_2:69474676-69474695 Msa0420750:five_prime_UTR 35.0%
AGAAATCTAGCAGTCTAGTT+TGG - chr3_2:69474795-69474814 Msa0420750:five_prime_UTR 35.0%
TGAAAATATTGCCATGATGG+GGG + chr3_2:69474941-69474960 None:intergenic 35.0%
GGATGAAAATATTGCCATGA+TGG + chr3_2:69474944-69474963 None:intergenic 35.0%
TTGTCGATCTCTTCATTAGT+TGG + chr3_2:69475151-69475170 None:intergenic 35.0%
AAGGATACAAGGTTCTCAAA+AGG + chr3_2:69475243-69475262 None:intergenic 35.0%
ATAGCAGATTTAATAGCAGC+CGG + chr3_2:69475274-69475293 None:intergenic 35.0%
! GTCTGAAGTTGAGATTTCTT+GGG + chr3_2:69475357-69475376 None:intergenic 35.0%
! AGTCTGAAGTTGAGATTTCT+TGG + chr3_2:69475358-69475377 None:intergenic 35.0%
TTTGTGGGATTGAGGAATTG+AGG + chr3_2:69473574-69473593 None:intergenic 40.0%
GAATGAGATTTGTGGGATTG+AGG + chr3_2:69473582-69473601 None:intergenic 40.0%
GAAGAACAGAAAGCCAATTG+GGG + chr3_2:69473788-69473807 None:intergenic 40.0%
GGAAGAACAGAAAGCCAATT+GGG + chr3_2:69473789-69473808 None:intergenic 40.0%
AGGAAGAACAGAAAGCCAAT+TGG + chr3_2:69473790-69473809 None:intergenic 40.0%
GAATTGGTTGTTGGAGATGA+AGG - chr3_2:69473912-69473931 Msa0420750:intron 40.0%
TTTGATGCAGTAGCCTCATT+TGG + chr3_2:69474083-69474102 None:intergenic 40.0%
! CTTGTTTGGTTCAATCAACG+GGG - chr3_2:69474342-69474361 Msa0420750:five_prime_UTR 40.0%
! TTGTTTGGTTCAATCAACGG+GGG - chr3_2:69474343-69474362 Msa0420750:five_prime_UTR 40.0%
TTAGAAGCAGGAAGAACATG+AGG + chr3_2:69474721-69474740 None:intergenic 40.0%
!! CTTAACTTTTAGGTCTCCAG+TGG - chr3_2:69474873-69474892 Msa0420750:five_prime_UTR 40.0%
GGATGTGTAATATAAGCCAC+TGG + chr3_2:69474892-69474911 None:intergenic 40.0%
TACAATGGTATTTGGCCCTT+TGG + chr3_2:69474965-69474984 None:intergenic 40.0%
ATCTCGGGTACAATGGTATT+TGG + chr3_2:69474973-69474992 None:intergenic 40.0%
!! CTTTAGAATCTCGGGTACAA+TGG + chr3_2:69474980-69474999 None:intergenic 40.0%
CATTGTACCCGAGATTCTAA+AGG - chr3_2:69474978-69474997 Msa0420750:five_prime_UTR 40.0%
CATGTCAATCCCTCACATTT+CGG - chr3_2:69475207-69475226 Msa0420750:five_prime_UTR 40.0%
ATGTCAATCCCTCACATTTC+GGG - chr3_2:69475208-69475227 Msa0420750:five_prime_UTR 40.0%
TGAGAACCTTGTATCCTTCT+TGG - chr3_2:69475245-69475264 Msa0420750:five_prime_UTR 40.0%
AAATCTGCTATCATGACCAC+AGG - chr3_2:69475283-69475302 Msa0420750:five_prime_UTR 40.0%
CAAGAGTTTCTTCTTACCTG+TGG + chr3_2:69475302-69475321 None:intergenic 40.0%
TTTGTGGGATTGAGGAATTG+AGG + chr3_2:69473574-69473593 None:intergenic 40.0%
GAATGAGATTTGTGGGATTG+AGG + chr3_2:69473582-69473601 None:intergenic 40.0%
GAAGAACAGAAAGCCAATTG+GGG + chr3_2:69473788-69473807 None:intergenic 40.0%
GGAAGAACAGAAAGCCAATT+GGG + chr3_2:69473789-69473808 None:intergenic 40.0%
AGGAAGAACAGAAAGCCAAT+TGG + chr3_2:69473790-69473809 None:intergenic 40.0%
GAATTGGTTGTTGGAGATGA+AGG - chr3_2:69473912-69473931 Msa0420750:intron 40.0%
TTTGATGCAGTAGCCTCATT+TGG + chr3_2:69474083-69474102 None:intergenic 40.0%
! CTTGTTTGGTTCAATCAACG+GGG - chr3_2:69474342-69474361 Msa0420750:five_prime_UTR 40.0%
! TTGTTTGGTTCAATCAACGG+GGG - chr3_2:69474343-69474362 Msa0420750:five_prime_UTR 40.0%
TTAGAAGCAGGAAGAACATG+AGG + chr3_2:69474721-69474740 None:intergenic 40.0%
!! CTTAACTTTTAGGTCTCCAG+TGG - chr3_2:69474873-69474892 Msa0420750:five_prime_UTR 40.0%
GGATGTGTAATATAAGCCAC+TGG + chr3_2:69474892-69474911 None:intergenic 40.0%
TACAATGGTATTTGGCCCTT+TGG + chr3_2:69474965-69474984 None:intergenic 40.0%
ATCTCGGGTACAATGGTATT+TGG + chr3_2:69474973-69474992 None:intergenic 40.0%
!! CTTTAGAATCTCGGGTACAA+TGG + chr3_2:69474980-69474999 None:intergenic 40.0%
CATTGTACCCGAGATTCTAA+AGG - chr3_2:69474978-69474997 Msa0420750:five_prime_UTR 40.0%
CATGTCAATCCCTCACATTT+CGG - chr3_2:69475207-69475226 Msa0420750:five_prime_UTR 40.0%
ATGTCAATCCCTCACATTTC+GGG - chr3_2:69475208-69475227 Msa0420750:five_prime_UTR 40.0%
TGAGAACCTTGTATCCTTCT+TGG - chr3_2:69475245-69475264 Msa0420750:five_prime_UTR 40.0%
AAATCTGCTATCATGACCAC+AGG - chr3_2:69475283-69475302 Msa0420750:five_prime_UTR 40.0%
CAAGAGTTTCTTCTTACCTG+TGG + chr3_2:69475302-69475321 None:intergenic 40.0%
ACCCAGAAAAGCTCAGTTCA+TGG - chr3_2:69473643-69473662 Msa0420750:CDS 45.0%
! GCCATGAACTGAGCTTTTCT+GGG + chr3_2:69473647-69473666 None:intergenic 45.0%
! TGCCATGAACTGAGCTTTTC+TGG + chr3_2:69473648-69473667 None:intergenic 45.0%
!! TGGCAATGATGGACCAAATG+AGG - chr3_2:69474067-69474086 Msa0420750:five_prime_UTR 45.0%
! CTGCATCAAATCTAGCTCCT+TGG - chr3_2:69474093-69474112 Msa0420750:intron 45.0%
GCTCCTTGGTATATCACAGT+TGG - chr3_2:69474107-69474126 Msa0420750:intron 45.0%
GCACCAACTGTGATATACCA+AGG + chr3_2:69474113-69474132 None:intergenic 45.0%
GAACTCTCTATCCATTGTGC+TGG + chr3_2:69474135-69474154 None:intergenic 45.0%
TGGCAACGGTTTAAGCTTCA+AGG - chr3_2:69474172-69474191 Msa0420750:intron 45.0%
TCTGGATCACAAGAAGGTGA+AGG - chr3_2:69474576-69474595 Msa0420750:five_prime_UTR 45.0%
CTGGATCACAAGAAGGTGAA+GGG - chr3_2:69474577-69474596 Msa0420750:five_prime_UTR 45.0%
ATAGTAGTGTGCCTGAATGG+AGG - chr3_2:69474604-69474623 Msa0420750:five_prime_UTR 45.0%
CTGAATGGAGGAGTGGATAA+GGG - chr3_2:69474616-69474635 Msa0420750:five_prime_UTR 45.0%
! TCTAGCAGTCTAGTTTGGTG+TGG - chr3_2:69474800-69474819 Msa0420750:five_prime_UTR 45.0%
CGAAATGTGAGGGATTGACA+TGG + chr3_2:69475209-69475228 None:intergenic 45.0%
CTGCAATTCCCGAAATGTGA+GGG + chr3_2:69475219-69475238 None:intergenic 45.0%
CTTGTATCCTTCTTGGAGTC+CGG - chr3_2:69475252-69475271 Msa0420750:five_prime_UTR 45.0%
ACCCAGAAAAGCTCAGTTCA+TGG - chr3_2:69473643-69473662 Msa0420750:CDS 45.0%
! GCCATGAACTGAGCTTTTCT+GGG + chr3_2:69473647-69473666 None:intergenic 45.0%
! TGCCATGAACTGAGCTTTTC+TGG + chr3_2:69473648-69473667 None:intergenic 45.0%
!! TGGCAATGATGGACCAAATG+AGG - chr3_2:69474067-69474086 Msa0420750:five_prime_UTR 45.0%
! CTGCATCAAATCTAGCTCCT+TGG - chr3_2:69474093-69474112 Msa0420750:intron 45.0%
GCTCCTTGGTATATCACAGT+TGG - chr3_2:69474107-69474126 Msa0420750:intron 45.0%
GCACCAACTGTGATATACCA+AGG + chr3_2:69474113-69474132 None:intergenic 45.0%
GAACTCTCTATCCATTGTGC+TGG + chr3_2:69474135-69474154 None:intergenic 45.0%
TGGCAACGGTTTAAGCTTCA+AGG - chr3_2:69474172-69474191 Msa0420750:intron 45.0%
TCTGGATCACAAGAAGGTGA+AGG - chr3_2:69474576-69474595 Msa0420750:five_prime_UTR 45.0%
CTGGATCACAAGAAGGTGAA+GGG - chr3_2:69474577-69474596 Msa0420750:five_prime_UTR 45.0%
ATAGTAGTGTGCCTGAATGG+AGG - chr3_2:69474604-69474623 Msa0420750:five_prime_UTR 45.0%
CTGAATGGAGGAGTGGATAA+GGG - chr3_2:69474616-69474635 Msa0420750:five_prime_UTR 45.0%
! TCTAGCAGTCTAGTTTGGTG+TGG - chr3_2:69474800-69474819 Msa0420750:five_prime_UTR 45.0%
CGAAATGTGAGGGATTGACA+TGG + chr3_2:69475209-69475228 None:intergenic 45.0%
CTGCAATTCCCGAAATGTGA+GGG + chr3_2:69475219-69475238 None:intergenic 45.0%
CTTGTATCCTTCTTGGAGTC+CGG - chr3_2:69475252-69475271 Msa0420750:five_prime_UTR 45.0%
! GTTGTTGTTGCTTGCTCTGC+TGG - chr3_2:69474047-69474066 Msa0420750:five_prime_UTR 50.0%
TGTGATCCAGAGTCCCATTC+TGG + chr3_2:69474567-69474586 None:intergenic 50.0%
TGGGACTCTGGATCACAAGA+AGG - chr3_2:69474570-69474589 Msa0420750:five_prime_UTR 50.0%
CCTTATCCACTCCTCCATTC+AGG + chr3_2:69474618-69474637 None:intergenic 50.0%
CCTGAATGGAGGAGTGGATA+AGG - chr3_2:69474615-69474634 Msa0420750:five_prime_UTR 50.0%
AGGAGTGGATAAGGGAGAAC+AGG - chr3_2:69474624-69474643 Msa0420750:five_prime_UTR 50.0%
GGAGAACAGGCTCTTATAGC+TGG - chr3_2:69474637-69474656 Msa0420750:five_prime_UTR 50.0%
!! GCTCTTATAGCTGGTGCTGT+AGG - chr3_2:69474646-69474665 Msa0420750:five_prime_UTR 50.0%
!! TATAGCTGGTGCTGTAGGAG+TGG - chr3_2:69474651-69474670 Msa0420750:five_prime_UTR 50.0%
TACTAAACCAGCCCCCATCA+TGG - chr3_2:69474927-69474946 Msa0420750:five_prime_UTR 50.0%
! AATATTGCCATGATGGGGGC+TGG + chr3_2:69474937-69474956 None:intergenic 50.0%
!! ACACCTGGTGCTGTGATATC+AGG + chr3_2:69475094-69475113 None:intergenic 50.0%
TATCACAGCACCAGGTGTGT+CGG - chr3_2:69475096-69475115 Msa0420750:five_prime_UTR 50.0%
TGGCTCTTCAGCTTCAGTGA+AGG + chr3_2:69475131-69475150 None:intergenic 50.0%
AGGGATTGACATGGATGTGC+CGG + chr3_2:69475200-69475219 None:intergenic 50.0%
CCTGCAATTCCCGAAATGTG+AGG + chr3_2:69475220-69475239 None:intergenic 50.0%
CCTCACATTTCGGGAATTGC+AGG - chr3_2:69475217-69475236 Msa0420750:five_prime_UTR 50.0%
CGGACTCCAAGAAGGATACA+AGG + chr3_2:69475254-69475273 None:intergenic 50.0%
! GTTGTTGTTGCTTGCTCTGC+TGG - chr3_2:69474047-69474066 Msa0420750:five_prime_UTR 50.0%
TGTGATCCAGAGTCCCATTC+TGG + chr3_2:69474567-69474586 None:intergenic 50.0%
TGGGACTCTGGATCACAAGA+AGG - chr3_2:69474570-69474589 Msa0420750:five_prime_UTR 50.0%
CCTTATCCACTCCTCCATTC+AGG + chr3_2:69474618-69474637 None:intergenic 50.0%
CCTGAATGGAGGAGTGGATA+AGG - chr3_2:69474615-69474634 Msa0420750:five_prime_UTR 50.0%
AGGAGTGGATAAGGGAGAAC+AGG - chr3_2:69474624-69474643 Msa0420750:five_prime_UTR 50.0%
GGAGAACAGGCTCTTATAGC+TGG - chr3_2:69474637-69474656 Msa0420750:five_prime_UTR 50.0%
!! GCTCTTATAGCTGGTGCTGT+AGG - chr3_2:69474646-69474665 Msa0420750:five_prime_UTR 50.0%
!! TATAGCTGGTGCTGTAGGAG+TGG - chr3_2:69474651-69474670 Msa0420750:five_prime_UTR 50.0%
TACTAAACCAGCCCCCATCA+TGG - chr3_2:69474927-69474946 Msa0420750:five_prime_UTR 50.0%
! AATATTGCCATGATGGGGGC+TGG + chr3_2:69474937-69474956 None:intergenic 50.0%
!! ACACCTGGTGCTGTGATATC+AGG + chr3_2:69475094-69475113 None:intergenic 50.0%
TATCACAGCACCAGGTGTGT+CGG - chr3_2:69475096-69475115 Msa0420750:five_prime_UTR 50.0%
TGGCTCTTCAGCTTCAGTGA+AGG + chr3_2:69475131-69475150 None:intergenic 50.0%
AGGGATTGACATGGATGTGC+CGG + chr3_2:69475200-69475219 None:intergenic 50.0%
CCTGCAATTCCCGAAATGTG+AGG + chr3_2:69475220-69475239 None:intergenic 50.0%
CCTCACATTTCGGGAATTGC+AGG - chr3_2:69475217-69475236 Msa0420750:five_prime_UTR 50.0%
CGGACTCCAAGAAGGATACA+AGG + chr3_2:69475254-69475273 None:intergenic 50.0%
GCTTGCTCTGCTGGCAATGA+TGG - chr3_2:69474056-69474075 Msa0420750:five_prime_UTR 55.0%
!! CACAGTTGGTGCCAGCACAA+TGG - chr3_2:69474121-69474140 Msa0420750:intron 55.0%
TGCAGCGAGCTTTGCCAGAA+TGG - chr3_2:69474550-69474569 Msa0420750:five_prime_UTR 55.0%
GCAGCGAGCTTTGCCAGAAT+GGG - chr3_2:69474551-69474570 Msa0420750:five_prime_UTR 55.0%
GCTTTGCCAGAATGGGACTC+TGG - chr3_2:69474558-69474577 Msa0420750:five_prime_UTR 55.0%
AGTGTGCCTGAATGGAGGAG+TGG - chr3_2:69474609-69474628 Msa0420750:five_prime_UTR 55.0%
CAGCCTGATATCACAGCACC+AGG - chr3_2:69475088-69475107 Msa0420750:five_prime_UTR 55.0%
GCTGCTATAACCGACACACC+TGG + chr3_2:69475109-69475128 None:intergenic 55.0%
ATAGCAGCCGGACTCCAAGA+AGG + chr3_2:69475262-69475281 None:intergenic 55.0%
GCTTGCTCTGCTGGCAATGA+TGG - chr3_2:69474056-69474075 Msa0420750:five_prime_UTR 55.0%
!! CACAGTTGGTGCCAGCACAA+TGG - chr3_2:69474121-69474140 Msa0420750:intron 55.0%
TGCAGCGAGCTTTGCCAGAA+TGG - chr3_2:69474550-69474569 Msa0420750:five_prime_UTR 55.0%
GCAGCGAGCTTTGCCAGAAT+GGG - chr3_2:69474551-69474570 Msa0420750:five_prime_UTR 55.0%
GCTTTGCCAGAATGGGACTC+TGG - chr3_2:69474558-69474577 Msa0420750:five_prime_UTR 55.0%
AGTGTGCCTGAATGGAGGAG+TGG - chr3_2:69474609-69474628 Msa0420750:five_prime_UTR 55.0%
CAGCCTGATATCACAGCACC+AGG - chr3_2:69475088-69475107 Msa0420750:five_prime_UTR 55.0%
GCTGCTATAACCGACACACC+TGG + chr3_2:69475109-69475128 None:intergenic 55.0%
ATAGCAGCCGGACTCCAAGA+AGG + chr3_2:69475262-69475281 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 69473495 69475479 69473495 ID=Msa0420750;Name=Msa0420750
chr3_2 mRNA 69473495 69475479 69473495 ID=Msa0420750-mRNA-1;Parent=Msa0420750;Name=Msa0420750-mRNA-1;_AED=0.36;_eAED=0.36;_QI=1260|1|1|1|0.66|0.75|4|128|104
chr3_2 exon 69473495 69473883 69473495 ID=Msa0420750-mRNA-1:exon:16525;Parent=Msa0420750-mRNA-1
chr3_2 exon 69473975 69474089 69473975 ID=Msa0420750-mRNA-1:exon:16524;Parent=Msa0420750-mRNA-1
chr3_2 exon 69474183 69474419 69474183 ID=Msa0420750-mRNA-1:exon:16523;Parent=Msa0420750-mRNA-1
chr3_2 exon 69474518 69475479 69474518 ID=Msa0420750-mRNA-1:exon:16522;Parent=Msa0420750-mRNA-1
chr3_2 five_prime_UTR 69474518 69475479 69474518 ID=Msa0420750-mRNA-1:five_prime_utr;Parent=Msa0420750-mRNA-1
chr3_2 five_prime_UTR 69474183 69474419 69474183 ID=Msa0420750-mRNA-1:five_prime_utr;Parent=Msa0420750-mRNA-1
chr3_2 five_prime_UTR 69474029 69474089 69474029 ID=Msa0420750-mRNA-1:five_prime_utr;Parent=Msa0420750-mRNA-1
chr3_2 CDS 69473975 69474028 69473975 ID=Msa0420750-mRNA-1:cds;Parent=Msa0420750-mRNA-1
chr3_2 CDS 69473623 69473883 69473623 ID=Msa0420750-mRNA-1:cds;Parent=Msa0420750-mRNA-1
chr3_2 three_prime_UTR 69473495 69473622 69473495 ID=Msa0420750-mRNA-1:three_prime_utr;Parent=Msa0420750-mRNA-1
Gene Sequence

>Msa0420750

ATGGCAATATTTTCATCCAAAGGGCCAAATACCATTGTACCCGAGATTCTAAAGCCTGATATCACAGCACCAGGTGTGTCGGTTATAGCAGCCTTCACTGAAGCTGAAGAGCCAACTAATGAAGAGATCGACAATCGCCGAATTCAATTTGTCTTACTCACCGGCACATCCATGTCAATCCCTCACATTTCGGGAATTGCAGGCCTTTTGAGAACCTTGTATCCTTCTTGGAGTCCGGCTGCTATTAAATCTGCTATCATGACCACAGGTAAGAAGAAACTCTTGAAGTTTCTCATACTTCATGTCATTTCTTGA
Protein sequence

>Msa0420750

MAIFSSKGPNTIVPEILKPDITAPGVSVIAAFTEAEEPTNEEIDNRRIQFVLLTGTSMSIPHISGIAGLLRTLYPSWSPAAIKSAIMTTGKKKLLKFLILHVIS*