AlfalfaGEDB | Msa1308000

M. sativa cultivar zhongmu-4 / Msa1308000

KEGG: -
GO: GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0046686 GO:0050896
PFAM: PF05922.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1308000	sp\|Q9FGU3\|SBT44_ARATH	49.653	721	334	9	26	720	24	741	0.0	689

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1308000	A0A396G9P4	94.414	734	27	1	1	720	4	737	0.0	1410

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001510	Msa1308000	0.828686	7.831594e-55	-8.615850e-47
Msa0002690	Msa1308000	0.816664	4.921406e-52	-8.615850e-47
Msa0002700	Msa1308000	0.808867	2.507267e-50	-8.615850e-47
Msa0003340	Msa1308000	0.813045	3.122492e-51	-8.615850e-47
Msa0004180	Msa1308000	0.839450	1.573431e-57	-8.615850e-47
Msa0007570	Msa1308000	0.858653	7.106741e-63	-8.615850e-47
Msa0007810	Msa1308000	0.800210	1.603637e-48	-8.615850e-47
Msa0011060	Msa1308000	0.809450	1.881362e-50	-8.615850e-47
Msa0011100	Msa1308000	0.822292	2.561472e-53	-8.615850e-47
Msa0011910	Msa1308000	0.817097	3.934765e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1308000	MtrunA17_Chr8g0334521	94.414	734	27	1	1	720	4	737	1410
Msa1308000	MtrunA17_Chr7g0227991	77.384	734	149	3	1	717	1	734	1155
Msa1308000	MtrunA17_Chr6g0470851	71.448	739	186	7	1	720	1	733	1051
Msa1308000	MtrunA17_Chr3g0079971	70.054	738	200	8	1	720	1	735	1028
Msa1308000	MtrunA17_Chr1g0170081	69.418	739	202	7	1	720	1	734	1017
Msa1308000	MtrunA17_Chr6g0470881	69.648	738	199	7	1	719	1	732	1012
Msa1308000	MtrunA17_Chr1g0146271	69.241	738	207	6	1	720	1	736	1001
Msa1308000	MtrunA17_Chr8g0334491	69.735	717	184	6	26	719	24	730	999
Msa1308000	MtrunA17_Chr1g0146251	68.784	740	209	5	1	720	1	738	999
Msa1308000	MtrunA17_Chr2g0301011	63.900	518	134	5	99	600	1	481	649

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1308000	AT5G59100.1	49.653	721	334	9	26	720	24	741	689
Msa1308000	AT3G46850.1	49.662	739	329	13	12	716	5	734	683
Msa1308000	AT3G46840.1	50.068	737	328	13	12	716	6	734	683
Msa1308000	AT5G59120.1	50.070	711	323	13	29	716	26	727	680
Msa1308000	AT5G58840.1	48.022	733	330	13	9	720	8	710	676
Msa1308000	AT5G59090.1	48.876	712	328	13	31	716	27	728	666
Msa1308000	AT5G58830.1	46.905	727	337	11	11	716	7	705	663
Msa1308000	AT5G59090.3	49.157	712	324	15	31	716	27	726	662
Msa1308000	AT5G59190.1	52.149	698	300	11	41	715	1	687	661
Msa1308000	AT5G59090.2	48.664	711	326	13	31	716	27	723	660

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Find 154 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTACATAGTGTACAT+GGG	0.679461	8_4:+4135526	Msa1308000:CDS
AAACCAGATATAAGTGCCCC+AGG	0.571838	8_4:+4137830	Msa1308000:CDS
AAAGGAAATACTCACGGTGC+AGG	0.518394	8_4:+4137256	Msa1308000:CDS
AACAAAATTAAACATATTAG+TGG	0.410582	8_4:+4138202	Msa1308000:CDS
AACGGCAAGTGGAAGAGAGG+TGG	0.600282	8_4:+4136995	Msa1308000:CDS
AATGCTCAAGCTTGCCCAAG+AGG	0.586375	8_4:+4137467	Msa1308000:CDS
AATGTCAACTCCATCAGCAA+TGG	0.625390	8_4:-4137153	None:intergenic
AATGTGAGGACATGCCATAG+AGG	0.691673	8_4:-4137945	None:intergenic
ACAAGAAGATTATTGGAGCA+CGG	0.641586	8_4:+4136913	Msa1308000:CDS
ACAAGGTCGTGGGACTTCCT+TGG	0.455572	8_4:+4135769	Msa1308000:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGTTGCAACAAATCATTGA+TGG	+	chr8_4:4135598-4135617	Msa1308000:CDS	30.0%
CATACTTAATGACCAACAAA+GGG	+	chr8_4:4135681-4135700	Msa1308000:CDS	30.0%
CAACAAAGGGAAAATCTTAT+TGG	+	chr8_4:4135694-4135713	Msa1308000:CDS	30.0%
ACTTGCAACAAGAAGATTAT+TGG	+	chr8_4:4136906-4136925	Msa1308000:CDS	30.0%
TTCAAAGATTGCTGTATACA+GGG	+	chr8_4:4137073-4137092	Msa1308000:CDS	30.0%
GACATTATCACAATCTCAAT+TGG	+	chr8_4:4137170-4137189	Msa1308000:CDS	30.0%
GTAGCAATACTAAATAACCA+AGG	-	chr8_4:4137329-4137348	None:intergenic	30.0%
TTCATTGATAAGCTCATTCT+TGG	+	chr8_4:4137368-4137387	Msa1308000:CDS	30.0%
ATTAATATTGTCCCCTCAAA+TGG	+	chr8_4:4137419-4137438	Msa1308000:CDS	30.0%
TTTGTATCCAAGTTAAGTGT+AGG	-	chr8_4:4137662-4137681	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	4135424	4138582	4135424	ID=Msa1308000;Name=Msa1308000
chr8_4	mRNA	4135424	4138582	4135424	ID=Msa1308000-mRNA-1;Parent=Msa1308000;Name=Msa1308000-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|0\|0\|1\|1\|1\|2\|0\|720
chr8_4	exon	4135424	4135895	4135424	ID=Msa1308000-mRNA-1:exon:1187;Parent=Msa1308000-mRNA-1
chr8_4	exon	4136892	4138582	4136892	ID=Msa1308000-mRNA-1:exon:1188;Parent=Msa1308000-mRNA-1
chr8_4	CDS	4135424	4135895	4135424	ID=Msa1308000-mRNA-1:cds;Parent=Msa1308000-mRNA-1
chr8_4	CDS	4136892	4138582	4136892	ID=Msa1308000-mRNA-1:cds;Parent=Msa1308000-mRNA-1

Gene Sequence

>Msa1308000

ATGGTCAAATATAATGCTATTTTATTCATATTTGTATCTGTTGTTTTGTTATCAATAATTTTCTTGGTGTGCGATGCCATAGAAAGTGATGATGAAAGCAGTAAAACTTACATAGTGTACATGGGCTCACTTCCTAAAAGAACATCATATTATCCAACCTCTCATCATCTCAGCATGTTGCAACAAATCATTGATGGTAGCAACGCAGAAAATCGCTTAGTTCGAAGTTACAACAGGAGCTTCAATGGTTTTGCTGCCATACTTAATGACCAACAAAGGGAAAATCTTATTGGCATGAGAGGTGTGGTCTCAGTTTTTCAATGCCAAAATTATCATCTAAAAACTACAAGGTCGTGGGACTTCCTTGGATTTCCTCAATCAATCAAAAGAGATCAATTCATTGAGAGTGGTTTGGTGGTTGGAGTCATAGATGGCGGAATATGGCCAGAATCTGAAAGTTTTACGGATAAAGGCGGTGGTAATTTCACTTGCAACAAGAAGATTATTGGAGCACGGTCGTACGACGGTGATCAGAGTGCAAGAGACTATGGTGGTCATGGAACCCACACGGCATCAACGGCAAGTGGAAGAGAGGTGGAGGGAGTGAGCTTTTATGATCTTGCAAAAGGCACCGCAAGAGGTGGAGTCCCGTCTTCAAAGATTGCTGTATACAGGGTATGCGACAAAGATGGTAACTGCAGCGGAAAGGATATTTTAGCTGCATTTGATGATGCCATTGCTGATGGAGTTGACATTATCACAATCTCAATTGGAAGCCAAATTGCTGTAGAATTTCTAAAAGATCCTATTGCCATTGGTTCTTTTCATGCCATGGAAAAGGAAATACTCACGGTGCAGGCCGCAGGAAACAGCGGTCCTAAACCAAGTAGTGTTTCTAGCGTAGCACCTTGGTTATTTAGTATTGCTACAACTACGGTAGATCATCAATTCATTGATAAGCTCATTCTTGGAAATGGAAAGACTTTTATTGGTAAGTCAATTAATATTGTCCCCTCAAATGGCACAAAATTCCCAATAGCTGTCCGTAATGCTCAAGCTTGCCCAAGAGGATATGGATCACCTGAAATGTGTGAGTGTATAGACAAAAATATGGTGAACGGTAAGCTTGTTTTGTGTGGAACACCAGGGGGTGAAGTATTGGCTTATGCGAATGGTGCAATTGGTTCAATCCTTAATGTTACACATTCTAAAAATGATGCTCCTCAGGTCTCTCTAAAGCCTACACTTAACTTGGATACAAATGACTATGTTCTTGTTCAATCTTACTCAAATTCCACAAAATACCCTGTAGCTGAAATTTTGAAGAGTGAAATCTTCCATGACAACAATGCTCCTACAGTTGCTTCTTTCTCTTCTCGTGGCCCAAATCCGTTGGTTCTAGAAATTATGAAACCAGATATAAGTGCCCCAGGAGTGGATATCTTGGCCGCATATTCACCTCTAGCGCCACCCTCAGACGATATAAATGACAAAAGACAGGTCAAATACAGCATAGAATCTGGAACCTCTATGGCATGTCCTCACATTGCTGGAGTTGCTGCATATGTGAAATCATTTCATCCAGATTGGTCGCCAGCATCTATCAAATCTGCCATCATGACCACGGCAAAACCGGTTAATGGTACTTATAATGATTTGGCAGGTGAGTTTGCTTATGGATCTGGGAATGTCAATCCAAAACAAGCTGTTGACCCTGGACTTGTTTATGACATCACTAAGGAAGATTATGTTTGGATGCTTTGTAATTATGGTTATGATACTAACAAAATTAAACATATTAGTGGAGAAAACTCAAGTTGCCATGGAGCTTCCAATAGATCCTTTGTGAAAGATATAAACTATCCAGCACTGGTGATTCCTGTTGAGTCTCATAAAAATTTCAATGTCAAGATTCATAGAACAGTTACAAATGTTGGCTCTCCCAACTCAAGCTACACTGCTACTGTCATCCCAATTCAAAATATAAAGATTAGTGTGGAGCCAAAAATTCTCTCATTCAGATCATTGAATGAGAAACAATCATTTGTTGTCAATGTTGTTGGGGGAGCAGAATCAAAACAAATGGTGTCTTCCTCCTCACTTGTTTGGTCAGATGGCACCCACAGGGTGAAGAGCCCAATCATCGTGCAAAGACTATCCTAA

Protein sequence

>Msa1308000

MVKYNAILFIFVSVVLLSIIFLVCDAIESDDESSKTYIVYMGSLPKRTSYYPTSHHLSMLQQIIDGSNAENRLVRSYNRSFNGFAAILNDQQRENLIGMRGVVSVFQCQNYHLKTTRSWDFLGFPQSIKRDQFIESGLVVGVIDGGIWPESESFTDKGGGNFTCNKKIIGARSYDGDQSARDYGGHGTHTASTASGREVEGVSFYDLAKGTARGGVPSSKIAVYRVCDKDGNCSGKDILAAFDDAIADGVDIITISIGSQIAVEFLKDPIAIGSFHAMEKEILTVQAAGNSGPKPSSVSSVAPWLFSIATTTVDHQFIDKLILGNGKTFIGKSINIVPSNGTKFPIAVRNAQACPRGYGSPEMCECIDKNMVNGKLVLCGTPGGEVLAYANGAIGSILNVTHSKNDAPQVSLKPTLNLDTNDYVLVQSYSNSTKYPVAEILKSEIFHDNNAPTVASFSSRGPNPLVLEIMKPDISAPGVDILAAYSPLAPPSDDINDKRQVKYSIESGTSMACPHIAGVAAYVKSFHPDWSPASIKSAIMTTAKPVNGTYNDLAGEFAYGSGNVNPKQAVDPGLVYDITKEDYVWMLCNYGYDTNKIKHISGENSSCHGASNRSFVKDINYPALVIPVESHKNFNVKIHRTVTNVGSPNSSYTATVIPIQNIKISVEPKILSFRSLNEKQSFVVNVVGGAESKQMVSSSSLVWSDGTHRVKSPIIVQRLS*