AlfalfaGEDB | Msa0833630

M. sativa cultivar zhongmu-4 / Msa0833630

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0833630	XP_003611256.1	97.222	252	6	1	1	252	1	251	1.77e-175	493

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0833630	G7KG86	97.222	252	6	1	1	252	1	251	8.44e-176	493

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa0833630	0.805990	1.022397e-49	-8.615850e-47
Msa0001420	Msa0833630	0.872765	2.473668e-67	-8.615850e-47
Msa0001510	Msa0833630	0.876772	1.070126e-68	-8.615850e-47
Msa0001540	Msa0833630	0.895018	1.427077e-75	-8.615850e-47
Msa0002060	Msa0833630	0.815941	7.143438e-52	-8.615850e-47
Msa0002690	Msa0833630	0.808168	3.535427e-50	-8.615850e-47
Msa0002700	Msa0833630	0.800128	1.666592e-48	-8.615850e-47
Msa0004180	Msa0833630	0.812744	3.634937e-51	-8.615850e-47
Msa0006050	Msa0833630	0.834042	3.768018e-56	-8.615850e-47
Msa0007570	Msa0833630	0.827491	1.519379e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0833630	MtrunA17_Chr5g0398861	97.222	252	6	1	1	252	1	251	1.62e-179	493

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0833630	AT1G62780.1	62.302	252	80	3	1	252	1	237	6.72e-106	306

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Find 72 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCGAAGCTGTCCTAACT+CGG	0.570557	5_4:-6746330	None:intergenic
AAAGTAAACTTACATCACGA+AGG	0.630395	5_4:-6745589	None:intergenic
AAATCCACTATTGTGTGTAT+TGG	0.451283	5_4:+6745310	Msa0833630:CDS
AACCAAAATCTTGACATCAA+AGG	0.566416	5_4:+6746948	Msa0833630:CDS
AAGGCCATACAAGAAGGAAC+AGG	0.513579	5_4:+6746492	Msa0833630:CDS
AAGTAAACTTACATCACGAA+GGG	0.626470	5_4:-6745588	None:intergenic
AAGTATTCAATGAGGTCTTG+TGG	0.566777	5_4:-6746123	None:intergenic
AAGTGCACATGGTGCCAACC+AGG	0.543322	5_4:+6747148	Msa0833630:CDS
AATACATGGAGAAGCTCCGT+GGG	0.575310	5_4:+6747463	Msa0833630:CDS
AATCCGAGTTAGGACAGCTT+CGG	0.500307	5_4:+6746327	Msa0833630:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCCACTATTGTGTGTAT+TGG	+	chr5_4:6745310-6745329	Msa0833630:CDS	30.0%
AAGTAAACTTACATCACGAA+GGG	-	chr5_4:6745591-6745610	None:intergenic	30.0%
AAAGTAAACTTACATCACGA+AGG	-	chr5_4:6745592-6745611	None:intergenic	30.0%
TGTCAAGTAAGTATTCAATG+AGG	-	chr5_4:6746134-6746153	None:intergenic	30.0%
CAAAATTTCACAATGATCTC+CGG	+	chr5_4:6746601-6746620	Msa0833630:intron	30.0%
AATTGATCAAATCGCCAATT+TGG	-	chr5_4:6746788-6746807	None:intergenic	30.0%
AAATTGGCGATTTGATCAAT+TGG	+	chr5_4:6746787-6746806	Msa0833630:intron	30.0%
AACCAAAATCTTGACATCAA+AGG	+	chr5_4:6746948-6746967	Msa0833630:CDS	30.0%
TTCAATTGATATTACCTGGT+TGG	-	chr5_4:6747165-6747184	None:intergenic	30.0%
TTTGAACATAACAGAACTTG+TGG	+	chr5_4:6747374-6747393	Msa0833630:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	6745140	6747732	6745140	ID=Msa0833630;Name=Msa0833630
chr5_4	mRNA	6745140	6747732	6745140	ID=Msa0833630-mRNA-1;Parent=Msa0833630;Name=Msa0833630-mRNA-1;_AED=0.02;_eAED=0.02;_QI=80\|1\|1\|1\|1\|1\|8\|220\|252
chr5_4	exon	6745140	6745360	6745140	ID=Msa0833630-mRNA-1:exon:2941;Parent=Msa0833630-mRNA-1
chr5_4	exon	6745452	6745598	6745452	ID=Msa0833630-mRNA-1:exon:2942;Parent=Msa0833630-mRNA-1
chr5_4	exon	6746102	6746199	6746102	ID=Msa0833630-mRNA-1:exon:2943;Parent=Msa0833630-mRNA-1
chr5_4	exon	6746298	6746370	6746298	ID=Msa0833630-mRNA-1:exon:2944;Parent=Msa0833630-mRNA-1
chr5_4	exon	6746453	6746513	6746453	ID=Msa0833630-mRNA-1:exon:2945;Parent=Msa0833630-mRNA-1
chr5_4	exon	6746899	6746984	6746899	ID=Msa0833630-mRNA-1:exon:2946;Parent=Msa0833630-mRNA-1
chr5_4	exon	6747080	6747169	6747080	ID=Msa0833630-mRNA-1:exon:2947;Parent=Msa0833630-mRNA-1
chr5_4	exon	6747450	6747732	6747450	ID=Msa0833630-mRNA-1:exon:2948;Parent=Msa0833630-mRNA-1
chr5_4	five_prime_UTR	6745140	6745219	6745140	ID=Msa0833630-mRNA-1:five_prime_utr;Parent=Msa0833630-mRNA-1
chr5_4	CDS	6745220	6745360	6745220	ID=Msa0833630-mRNA-1:cds;Parent=Msa0833630-mRNA-1
chr5_4	CDS	6745452	6745598	6745452	ID=Msa0833630-mRNA-1:cds;Parent=Msa0833630-mRNA-1
chr5_4	CDS	6746102	6746199	6746102	ID=Msa0833630-mRNA-1:cds;Parent=Msa0833630-mRNA-1
chr5_4	CDS	6746298	6746370	6746298	ID=Msa0833630-mRNA-1:cds;Parent=Msa0833630-mRNA-1
chr5_4	CDS	6746453	6746513	6746453	ID=Msa0833630-mRNA-1:cds;Parent=Msa0833630-mRNA-1
chr5_4	CDS	6746899	6746984	6746899	ID=Msa0833630-mRNA-1:cds;Parent=Msa0833630-mRNA-1
chr5_4	CDS	6747080	6747169	6747080	ID=Msa0833630-mRNA-1:cds;Parent=Msa0833630-mRNA-1
chr5_4	CDS	6747450	6747512	6747450	ID=Msa0833630-mRNA-1:cds;Parent=Msa0833630-mRNA-1
chr5_4	three_prime_UTR	6747513	6747732	6747513	ID=Msa0833630-mRNA-1:three_prime_utr;Parent=Msa0833630-mRNA-1

Gene Sequence

>Msa0833630

ATGGCTACGCTTAACTTTGGCATAGCCACAACAAGAAGTTTGAATGCTACTTCTGGTTTTCGAACTAGAACCAAATCATGGAGCAGAGGCAAATCCACTATTGTGTGTATTGGTCCAGGTCCTATTGGTCCTGTTGATTGGGACCCAGAAGGTGTACTAGGCCGGCCCAATACTGGCCACCTTGCTAGGCTTGAGTTTAAGAGACGTCTTGAGAAAGATTCTGAAGCTCGAGAAGCCTTTGAGCGTCAAGTCCGTGAAGAGAAAGAACGTCGTCGAGCCCTTCGTGATTCTCGTGATGTCCCTGATACTCCACAAGACCTCATTGAATACTTACTTGACACTGAAGCTCAGGAGATTGAATTTGAGATTGCAAGAATGAGACCACGATTAAACGACGAATTCTTTTTGGCATTAAAATCCGAGTTAGGACAGCTTCGGTTTGCTGTTAACAAAACACAGCTTACGGAGGATAGATTGACGGAGCTGGAGGCTTTAGAGAAGGCCATACAAGAAGGAACAGATGCCTATGATAAGATGCAAGCTCAGCTCATAAAAGCCAAGGAAAACCTAACCAAAATCTTGACATCAAAGGATGTCAAAGCAACTTTGCTGGAGTTGGTTGAGCGCAACGAGATTAATCGATCATTGTTGACCCTTCTCGATGAAAACATATCAAGTGCACATGGTGCCAACCAGAAGCAAGCTGCAGAATACATGGAGAAGCTCCGTGGGGCTGTTCTCAAATACATTACAGTTTAG

Protein sequence

>Msa0833630

MATLNFGIATTRSLNATSGFRTRTKSWSRGKSTIVCIGPGPIGPVDWDPEGVLGRPNTGHLARLEFKRRLEKDSEAREAFERQVREEKERRRALRDSRDVPDTPQDLIEYLLDTEAQEIEFEIARMRPRLNDEFFLALKSELGQLRFAVNKTQLTEDRLTELEALEKAIQEGTDAYDKMQAQLIKAKENLTKILTSKDVKATLLELVERNEINRSLLTLLDENISSAHGANQKQAAEYMEKLRGAVLKYITV*